pUC118-mCtnnb1-FLIP-FlpE-Puro
(Plasmid
#84541)
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PurposeCRISPR - FLIP-FlpE targeting vector for mouse beta-catenin
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Depositing Lab
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Depositing OrganizationUniversity of Cambridge
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84541 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 84541-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 84541-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC118
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Backbone manufacturerTAKARA
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Vector typeMouse Targeting, Cre/Lox
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionssuggested bacterial strain for expression is Stellar (HST08)
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemouse beta-catenin FLIP-FlpE - Puro targeting vector
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SpeciesSynthetic
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Insert Size (bp)3903
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 84541-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 84541-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC118-mCtnnb1-FLIP-FlpE-Puro was a gift from Bon-Kyoung Koo (Addgene plasmid # 84541 ; http://n2t.net/addgene:84541 ; RRID:Addgene_84541) -
For your References section:
One-step generation of conditional and reversible gene knockouts. Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK. Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156. 10.1038/nmeth.4156 PubMed 28135257