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pCas9-HE
(Plasmid #109400)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109400 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 109400-D50 50 μg of DNA in Tris buffer $495
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DNA 109400-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    hCas9 Addgene ID 41815
  • Backbone manufacturer
    Church lab A
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HDR-Enhancer domain of human CtIP protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    888
  • Entrez Gene
    RBBP8 (a.k.a. COM1, CTIP, JAWAD, JWDS, RIM, SAE2, SCKL2)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note from depositor: The sequence used for the HDR enhancer domain of CtIP has several differences compared to the reference human CtIP cDNA sequence including T176A. These differences were present in the CtIP expression vector used to make the constructs and do not affect activity.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas9-HE was a gift from Jean-Paul Concordet (Addgene plasmid # 109400 ; http://n2t.net/addgene:109400 ; RRID:Addgene_109400)

  • For your References section:

    CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Charpentier M, Khedher AHY, Menoret S, Brion A, Lamribet K, Dardillac E, Boix C, Perrouault L, Tesson L, Geny S, De Cian A, Itier JM, Anegon I, Lopez B, Giovannangeli C, Concordet JP. Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7. 10.1038/s41467-018-03475-7 PubMed 29556040
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