pCY_gRNA_hPAX7-Pro_C5
(Plasmid #160456)

• Purpose: sgRNA targeting human PAX7 promoter
• Depositing Lab: April Pyle
• Publication: Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi: 10.1016/j.xpro.2020.100158. eCollection 2020 Dec 18.

Backbone

• Vector backbone: gRNA_Cloning Vector
• Backbone manufacturer: 41824
• Backbone size w/o insert (bp): 3910
• Total vector size (bp): 3973
• Modifications to backbone: The AflII site was destroyed during cloning which results in a loss of 4 bp from the original backbone (3914 bp)
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gRNA_hPAX7-Promoter_C5
• gRNA/shRNA sequence: GGGTCCGGAGAAAGAAGGCG
• Species: H. sapiens (human)
• GenBank ID: NM_001135254
• Promoter: U6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7: TAATACGACTCACTATAGGG

Resource Information

• Supplemental Documents:
  • pCY_gRNA_hPAX7-Pro_C5.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry