pCY_gRNA_hPAX7-Pro_C3
(Plasmid
#160454)
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PurposesgRNA targeting human PAX7 promoter
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Depositing Lab
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Depositing OrganizationUniversity of California, Los Angeles (UCLA)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160454 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 160454-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 160454-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonegRNA_Cloning Vector
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Backbone manufacturer41824
- Backbone size w/o insert (bp) 3910
- Total vector size (bp) 3973
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Modifications to backboneThe AflII site was destroyed during cloning which results in a loss of 4 bp from the original backbone (3914 bp)
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegRNA_hPAX7-Promoter_C3
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gRNA/shRNA sequenceGTCAAACGCGTCCAGAAGCT
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SpeciesH. sapiens (human)
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GenBank IDNM_001135254
- Promoter U6
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7: TAATACGACTCACTATAGGG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 160454-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 160454-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCY_gRNA_hPAX7-Pro_C3 was a gift from April Pyle (Addgene plasmid # 160454 ; http://n2t.net/addgene:160454 ; RRID:Addgene_160454) -
For your References section:
Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells. Xi H, Young CS, Pyle AD. STAR Protoc. 2020 Nov 5;1(3):100158. doi: 10.1016/j.xpro.2020.100158. eCollection 2020 Dec 18. 10.1016/j.xpro.2020.100158 PubMed 33377052
Map uploaded by the depositor.
