161,202 results

1. psPAX2

   Plasmid

   #12260

   Purpose

   2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Lentiviral; Packaging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMD2.G

   Plasmid

   #12259

   Purpose

   VSV-G envelope expressing plasmid

   Depositor

   Didier Trono

   Insert

   VSV G
   

   Use

   Lentiviral; Envelope

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2/9n

   Plasmid

   #112865

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Truncated P5

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2/8

   Plasmid

   #112864

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap8
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Truncated P5

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-GFP (PX458)

   Plasmid

   #48138

   Purpose

   Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tet-pLKO-puro

   Plasmid

   #21915

   Purpose

   3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

   Depositor

   Dmitri Wiederschain

   Article

   Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-VSV-G

   Plasmid

   #8454

   Purpose

   Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAdDeltaF6

   Plasmid

   #112867

   Purpose

   AAV helper plasmid

   Depositor

   James M. Wilson

   Insert

   E4, E2a and VA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry

    Plasmid

    #176016

    Purpose

    Mammalian expression of mCherry. Parton lab clone DFQ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PE6d

    Plasmid

    #207854

    Purpose

    Mammalian expression of PE6d prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6d
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid

    #91796

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Puro (PX459) V2.0

    Plasmid

    #62988

    Purpose

    Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9-2A-Puro V2.0
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUCmini-iCAP-AAV.CAP-B22

    Plasmid

    #175005

    Purpose

    non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

    Depositor

    Viviana Gradinaru

    Article

    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

    Insert

    Synthetic construct isolate AAV.CAP-B22 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    7 amino acid substitution between VP1 452 and VP1…

    Promoter

    p41

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-a and hU6

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. phCMV-229E-D15

    Plasmid

    #188908

    Purpose

    express truncated 229E spike for pseudovirus production

    Depositor

    David Nemazee

    Insert

    229E spike (S 229E)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c-terminal 15aa deletion

    Promoter

    CMV

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-puro-shNT

    Plasmid

    #109012

    Purpose

    short-hairpin knockdown of targeted gene

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. HDM_SARS2_Spike_del21_D614G

    Plasmid

    #158762

    Purpose

    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS2-Spike_del21_D614G (S Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 1257-1278, changed aspartic a…

    Promoter

    CMV

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.3_CoV1_D28

    Plasmid

    #170447

    Purpose

    Express SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    SARS-CoV-1 spike D28 (S SARS-CoV-1)
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    last 28aa deletion in c-terminal tail

    Promoter

    CMV

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.3_MERS_D12

    Plasmid

    #170448

    Purpose

    Express MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    MERS spike D12 (S MERS)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    last 12aa deletion in c-terminal tail

    Promoter

    CMV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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