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pX330-U6-Chimeric_BB-CBh-hSpCas9 Citations (802)

Plasmid Article: Multiplex Genome Engineering Using CRISPR/Cas Systems.
Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F Science. 2013 Jan 3.
PubMed Journal

Articles Citing pX330-U6-Chimeric_BB-CBh-hSpCas9

Articles
AP-1/IRF-3 Targeted Anti-Inflammatory Activity of Andrographolide Isolated from Andrographis paniculata. Shen T, Yang WS, Yi YS, Sung GH, Rhee MH, Poo H, Kim MY, Kim KW, Kim JH, Cho JY. Evid Based Complement Alternat Med. 2013;2013:210736. doi: 10.1155/2013/210736. Epub 2013 Jun 6. PubMed
CRISPR/Cas9 systems targeting beta-globin and CCR5 genes have substantial off-target activity. Cradick TJ, Fine EJ, Antico CJ, Bao G. Nucleic Acids Res. 2013 Nov;41(20):9584-92. doi: 10.1093/nar/gkt714. Epub 2013 Aug 11. PubMed
CRISPR/Cas-induced double-strand breaks boost the frequency of gene replacements for humanizing the mouse Cnr2 gene. Gennequin B, Otte DM, Zimmer A. Biochem Biophys Res Commun. 2013 Nov 29;441(4):815-9. doi: 10.1016/j.bbrc.2013.10.138. Epub 2013 Nov 6. PubMed
Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M. Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355. PubMed

Associated Plasmids

Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Bassett AR, Tibbit C, Ponting CP, Liu JL. Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi: 10.1242/bio.20137120. PubMed

Associated Plasmids

Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes. Mashiko D, Young SA, Muto M, Kato H, Nozawa K, Ogawa M, Noda T, Kim YJ, Satouh Y, Fujihara Y, Ikawa M. Dev Growth Differ. 2014 Jan;56(1):122-9. doi: 10.1111/dgd.12113. Epub 2013 Dec 26. PubMed
A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. Kranz D, Boutros M. EMBO J. 2014 Feb 3;33(3):181-97. doi: 10.1002/embj.201385686. Epub 2014 Jan 17. PubMed
IgH class switching exploits a general property of two DNA breaks to be joined in cis over long chromosomal distances. Gostissa M, Schwer B, Chang A, Dong J, Meyers RM, Marecki GT, Choi VW, Chiarle R, Zarrin AA, Alt FW. Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2644-9. doi: 10.1073/pnas.1324176111. Epub 2014 Feb 3. PubMed
EFCAB7 and IQCE regulate hedgehog signaling by tethering the EVC-EVC2 complex to the base of primary cilia. Pusapati GV, Hughes CE, Dorn KV, Zhang D, Sugianto P, Aravind L, Rohatgi R. Dev Cell. 2014 Mar 10;28(5):483-96. doi: 10.1016/j.devcel.2014.01.021. Epub 2014 Feb 27. PubMed
Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. Hendel A, Kildebeck EJ, Fine EJ, Clark JT, Punjya N, Sebastiano V, Bao G, Porteus MH. Cell Rep. 2014 Apr 10;7(1):293-305. doi: 10.1016/j.celrep.2014.02.040. Epub 2014 Mar 27. PubMed
Targeted genome editing of sweet orange using Cas9/sgRNA. Jia H, Wang N. PLoS One. 2014 Apr 7;9(4):e93806. doi: 10.1371/journal.pone.0093806. eCollection 2014. PubMed
Targeted mutagenesis using CRISPR/Cas system in medaka. Ansai S, Kinoshita M. Biol Open. 2014 Apr 11;3(5):362-71. doi: 10.1242/bio.20148177. PubMed
CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function. Park A, Won ST, Pentecost M, Bartkowski W, Lee B. PLoS One. 2014 Apr 17;9(4):e95101. doi: 10.1371/journal.pone.0095101. eCollection 2014. PubMed

Associated Plasmids

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Wu X, Scott DA, Kriz AJ, Chiu AC, Hsu PD, Dadon DB, Cheng AW, Trevino AE, Konermann S, Chen S, Jaenisch R, Zhang F, Sharp PA. Nat Biotechnol. 2014 Jul;32(7):670-6. doi: 10.1038/nbt.2889. Epub 2014 Apr 20. PubMed
Targeted genomic rearrangements using CRISPR/Cas technology. Choi PS, Meyerson M. Nat Commun. 2014 Apr 24;5:3728. doi: 10.1038/ncomms4728. PubMed
An RNF168 fragment defective for focal accumulation at DNA damage is proficient for inhibition of homologous recombination in BRCA1 deficient cells. Munoz MC, Yanez DA, Stark JM. Nucleic Acids Res. 2014 Jul;42(12):7720-33. doi: 10.1093/nar/gku421. Epub 2014 May 14. PubMed
Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mizuno S, Dinh TT, Kato K, Mizuno-Iijima S, Tanimoto Y, Daitoku Y, Hoshino Y, Ikawa M, Takahashi S, Sugiyama F, Yagami K. Mamm Genome. 2014 Aug;25(7-8):327-34. doi: 10.1007/s00335-014-9524-0. Epub 2014 May 31. PubMed
LKB1 loss at transcriptional level promotes tumor malignancy and poor patient outcomes in colorectal cancer. He TY, Tsai LH, Huang CC, Chou MC, Lee H. Ann Surg Oncol. 2014 Dec;21 Suppl 4:S703-10. doi: 10.1245/s10434-014-3824-1. Epub 2014 May 31. PubMed
Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system. Ghorbal M, Gorman M, Macpherson CR, Martins RM, Scherf A, Lopez-Rubio JJ. Nat Biotechnol. 2014 Aug;32(8):819-21. doi: 10.1038/nbt.2925. Epub 2014 Jun 1. PubMed
Characterization of genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells. Canver MC, Bauer DE, Dass A, Yien YY, Chung J, Masuda T, Maeda T, Paw BH, Orkin SH. J Biol Chem. 2014 Aug 1;289(31):21312-24. doi: 10.1074/jbc.M114.564625. Epub 2014 Jun 6. PubMed
An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells. Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D. Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. PubMed

Associated Plasmids

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. PubMed

Associated Plasmids

Efficient editing of malaria parasite genome using the CRISPR/Cas9 system. Zhang C, Xiao B, Jiang Y, Zhao Y, Li Z, Gao H, Ling Y, Wei J, Li S, Lu M, Su XZ, Cui H, Yuan J. MBio. 2014 Jul 1;5(4):e01414-14. doi: 10.1128/mBio.01414-14. PubMed
Generating genetically modified mice using CRISPR/Cas-mediated genome engineering. Yang H, Wang H, Jaenisch R. Nat Protoc. 2014 Aug;9(8):1956-68. doi: 10.1038/nprot.2014.134. Epub 2014 Jul 24. PubMed
DNAVaxDB: the first web-based DNA vaccine database and its data analysis. Racz R, Li X, Patel M, Xiang Z, He Y. BMC Bioinformatics. 2014;15 Suppl 4:S2. doi: 10.1186/1471-2105-15-S4-S2. Epub 2014 Mar 19. PubMed
RIPK1 maintains epithelial homeostasis by inhibiting apoptosis and necroptosis. Dannappel M, Vlantis K, Kumari S, Polykratis A, Kim C, Wachsmuth L, Eftychi C, Lin J, Corona T, Hermance N, Zelic M, Kirsch P, Basic M, Bleich A, Kelliher M, Pasparakis M. Nature. 2014 Sep 4;513(7516):90-4. doi: 10.1038/nature13608. Epub 2014 Aug 17. PubMed
Understanding functional miRNA-target interactions in vivo by site-specific genome engineering. Bassett AR, Azzam G, Wheatley L, Tibbit C, Rajakumar T, McGowan S, Stanger N, Ewels PA, Taylor S, Ponting CP, Liu JL, Sauka-Spengler T, Fulga TA. Nat Commun. 2014 Aug 19;5:4640. doi: 10.1038/ncomms5640. PubMed
CTCF controls HOXA cluster silencing and mediates PRC2-repressive higher-order chromatin structure in NT2/D1 cells. Xu M, Zhao GN, Lv X, Liu G, Wang LY, Hao DL, Wang J, Liu DP, Liang CC. Mol Cell Biol. 2014 Oct;34(20):3867-79. doi: 10.1128/MCB.00567-14. Epub 2014 Aug 18. PubMed
CRISPR/Cas9-mediated gene knock-down in post-mitotic neurons. Straub C, Granger AJ, Saulnier JL, Sabatini BL. PLoS One. 2014 Aug 20;9(8):e105584. doi: 10.1371/journal.pone.0105584. eCollection 2014. PubMed
Saturation editing of genomic regions by multiplex homology-directed repair. Findlay GM, Boyle EA, Hause RJ, Klein JC, Shendure J. Nature. 2014 Sep 4;513(7516):120-3. doi: 10.1038/nature13695. PubMed
Heat-stable molecule derived from Streptococcus cristatus induces APOBEC3 expression and inhibits HIV-1 replication. Wang Z, Luo Y, Shao Q, Kinlock BL, Wang C, Hildreth JE, Xie H, Liu B. PLoS One. 2014 Aug 28;9(8):e106078. doi: 10.1371/journal.pone.0106078. eCollection 2014. PubMed
Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single-guide RNA and carbohydrate selection. Li P, Estrada JL, Burlak C, Montgomery J, Butler JR, Santos RM, Wang ZY, Paris LL, Blankenship RL, Downey SM, Tector M, Tector AJ. Xenotransplantation. 2015 Jan-Feb;22(1):20-31. doi: 10.1111/xen.12131. Epub 2014 Sep 2. PubMed
Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter. Zhang JH, Pandey M, Kahler JF, Loshakov A, Harris B, Dagur PK, Mo Y, Simonds WF. J Biotechnol. 2014 Sep 2. pii: S0168-1656(14)00838-4. doi: 10.1016/j.jbiotec.2014.08.033. PubMed

Associated Plasmids

Single-step generation of rabbits carrying a targeted allele of the tyrosinase gene using CRISPR/Cas9. Honda A, Hirose M, Sankai T, Yasmin L, Yuzawa K, Honsho K, Izu H, Iguchi A, Ikawa M, Ogura A. Exp Anim. 2015;64(1):31-7. doi: 10.1538/expanim.14-0034. Epub 2014 Sep 8. PubMed
CRISPR/Cas9 mediated generation of stable chondrocyte cell lines with targeted gene knockouts; analysis of an aggrecan knockout cell line. Yang M, Zhang L, Stevens J, Gibson G. Bone. 2014 Dec;69:118-25. doi: 10.1016/j.bone.2014.09.005. Epub 2014 Sep 26. PubMed
Mouse Genome Editing Using the CRISPR/Cas System. Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB. Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83. PubMed

Associated Plasmids

Efficient gene targeting in golden Syrian hamsters by the CRISPR/Cas9 system. Fan Z, Li W, Lee SR, Meng Q, Shi B, Bunch TD, White KL, Kong IK, Wang Z. PLoS One. 2014 Oct 9;9(10):e109755. doi: 10.1371/journal.pone.0109755. eCollection 2014. PubMed
Easy quantitative assessment of genome editing by sequence trace decomposition. Brinkman EK, Chen T, Amendola M, van Steensel B. Nucleic Acids Res. 2014 Dec 16;42(22):e168. doi: 10.1093/nar/gku936. Epub 2014 Oct 9. PubMed
Control of cell identity genes occurs in insulated neighborhoods in mammalian chromosomes. Dowen JM, Fan ZP, Hnisz D, Ren G, Abraham BJ, Zhang LN, Weintraub AS, Schuijers J, Lee TI, Zhao K, Young RA. Cell. 2014 Oct 9;159(2):374-87. doi: 10.1016/j.cell.2014.09.030. PubMed
In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura A. Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22. PubMed

Associated Plasmids

Creating class I MHC-null pigs using guide RNA and the Cas9 endonuclease. Reyes LM, Estrada JL, Wang ZY, Blosser RJ, Smith RF, Sidner RA, Paris LL, Blankenship RL, Ray CN, Miner AC, Tector M, Tector AJ. J Immunol. 2014 Dec 1;193(11):5751-7. doi: 10.4049/jimmunol.1402059. Epub 2014 Oct 22. PubMed
Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks. Renouf B, Piganeau M, Ghezraoui H, Jasin M, Brunet E. Methods Enzymol. 2014;546:251-71. doi: 10.1016/B978-0-12-801185-0.00012-X. PubMed
CRISPR/Cas9-based genome editing in mice by single plasmid injection. Fujihara Y, Ikawa M. Methods Enzymol. 2014;546:319-36. doi: 10.1016/B978-0-12-801185-0.00015-5. PubMed
Genome engineering using CRISPR-Cas9 system. Cong L, Zhang F. Methods Mol Biol. 2015;1239:197-217. doi: 10.1007/978-1-4939-1862-1_10. PubMed
Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9. Nakade S, Tsubota T, Sakane Y, Kume S, Sakamoto N, Obara M, Daimon T, Sezutsu H, Yamamoto T, Sakuma T, Suzuki KT. Nat Commun. 2014 Nov 20;5:5560. doi: 10.1038/ncomms6560. PubMed
The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3. Liew GM, Ye F, Nager AR, Murphy JP, Lee JS, Aguiar M, Breslow DK, Gygi SP, Nachury MV. Dev Cell. 2014 Nov 10;31(3):265-78. doi: 10.1016/j.devcel.2014.09.004. Epub 2014 Oct 30. PubMed
Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Wu Y, Zhou H, Fan X, Zhang Y, Zhang M, Wang Y, Xie Z, Bai M, Yin Q, Liang D, Tang W, Liao J, Zhou C, Liu W, Zhu P, Guo H, Pan H, Wu C, Shi H, Wu L, Tang F, Li J. Cell Res. 2015 Jan;25(1):67-79. doi: 10.1038/cr.2014.160. Epub 2014 Dec 5. PubMed
Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing. Roy A, Goodman JH, Begum G, Donnelly BF, Pittman G, Weinman EJ, Sun D, Subramanya AR. Am J Physiol Renal Physiol. 2015 Feb 15;308(4):F366-76. doi: 10.1152/ajprenal.00612.2014. Epub 2014 Dec 4. PubMed
Engineering herpes simplex viruses by infection-transfection methods including recombination site targeting by CRISPR/Cas9 nucleases. Russell TA, Stefanovic T, Tscharke DC. J Virol Methods. 2015 Mar;213:18-25. doi: 10.1016/j.jviromet.2014.11.009. Epub 2014 Dec 3. PubMed
ST3Gal-4 is the primary sialyltransferase regulating the synthesis of E-, P-, and L-selectin ligands on human myeloid leukocytes. Mondal N, Buffone A Jr, Stolfa G, Antonopoulos A, Lau JT, Haslam SM, Dell A, Neelamegham S. Blood. 2015 Jan 22;125(4):687-96. doi: 10.1182/blood-2014-07-588590. Epub 2014 Dec 10. PubMed
Amphipathic alpha-helices in apolipoproteins are crucial to the formation of infectious hepatitis C virus particles. Fukuhara T, Wada M, Nakamura S, Ono C, Shiokawa M, Yamamoto S, Motomura T, Okamoto T, Okuzaki D, Yamamoto M, Saito I, Wakita T, Koike K, Matsuura Y. PLoS Pathog. 2014 Dec 11;10(12):e1004534. doi: 10.1371/journal.ppat.1004534. eCollection 2014 Dec. PubMed
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. Frock RL, Hu J, Meyers RM, Ho YJ, Kii E, Alt FW. Nat Biotechnol. 2015 Feb;33(2):179-86. doi: 10.1038/nbt.3101. Epub 2014 Dec 15. PubMed
Generation of genomic deletions in mammalian cell lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J Vis Exp. 2014 Jan 3;(95). doi: 10.3791/52118. PubMed
Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing. Parikh BA, Beckman DL, Patel SJ, White JM, Yokoyama WM. PLoS One. 2015 Jan 14;10(1):e0116484. doi: 10.1371/journal.pone.0116484. eCollection 2015. PubMed
Improving gene targeting efficiency on pig IGF2 mediated by ZFNs and CRISPR/Cas9 by using SSA reporter system. Jinqing W, Gui M, Zhiguo L, Yaosheng C, Peiqing C, Zuyong H. Yi Chuan. 2015 Jan;37(1):55-62. doi: 10.16288/j.yczz.2015.01.008. PubMed
Site- and allele-specific polycomb dysregulation in T-cell leukaemia. Navarro JM, Touzart A, Pradel LC, Loosveld M, Koubi M, Fenouil R, Le Noir S, Maqbool MA, Morgado E, Gregoire C, Jaeger S, Mamessier E, Pignon C, Hacein-Bey-Abina S, Malissen B, Gut M, Gut IG, Dombret H, Macintyre EA, Howe SJ, Gaspar HB, Thrasher AJ, Ifrah N, Payet-Bornet D, Duprez E, Andrau JC, Asnafi V, Nadel B. Nat Commun. 2015 Jan 23;6:6094. doi: 10.1038/ncomms7094. PubMed
DNA damage response factors from diverse pathways, including DNA crosslink repair, mediate alternative end joining. Howard SM, Yanez DA, Stark JM. PLoS Genet. 2015 Jan 28;11(1):e1004943. doi: 10.1371/journal.pgen.1004943. eCollection 2015 Jan. PubMed
Mammalian polymerase theta promotes alternative NHEJ and suppresses recombination. Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E, Sfeir A. Nature. 2015 Feb 12;518(7538):254-7. doi: 10.1038/nature14157. Epub 2015 Feb 2. PubMed

Associated Plasmids

Modeling of the human alveolar rhabdomyosarcoma Pax3-Foxo1 chromosome translocation in mouse myoblasts using CRISPR-Cas9 nuclease. Lagutina IV, Valentine V, Picchione F, Harwood F, Valentine MB, Villarejo-Balcells B, Carvajal JJ, Grosveld GC. PLoS Genet. 2015 Feb 6;11(2):e1004951. doi: 10.1371/journal.pgen.1004951. eCollection 2015. PubMed
Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast. Lee NC, Larionov V, Kouprina N. Nucleic Acids Res. 2015 Apr 30;43(8):e55. doi: 10.1093/nar/gkv112. Epub 2015 Feb 17. PubMed
Evaluation of human and non-human primate antibody binding to pig cells lacking GGTA1/CMAH/beta4GalNT2 genes. Estrada JL, Martens G, Li P, Adams A, Newell KA, Ford ML, Butler JR, Sidner R, Tector M, Tector J. Xenotransplantation. 2015 May;22(3):194-202. doi: 10.1111/xen.12161. Epub 2015 Mar 1. PubMed
Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Hisano Y, Sakuma T, Nakade S, Ohga R, Ota S, Okamoto H, Yamamoto T, Kawahara A. Sci Rep. 2015 Mar 5;5:8841. doi: 10.1038/srep08841. PubMed
Hepatitis C virus RNA functionally sequesters miR-122. Luna JM, Scheel TK, Danino T, Shaw KS, Mele A, Fak JJ, Nishiuchi E, Takacs CN, Catanese MT, de Jong YP, Jacobson IM, Rice CM, Darnell RB. Cell. 2015 Mar 12;160(6):1099-110. doi: 10.1016/j.cell.2015.02.025. PubMed
Efficient human immunodeficiency virus (HIV-1) infection of cells lacking PDZD8. Zhang S, Sodroski J. Virology. 2015 Jul;481:73-8. doi: 10.1016/j.virol.2015.01.034. Epub 2015 Mar 11. PubMed
Selective Distal Enhancer Control of the Mmp13 Gene Identified through Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Genomic Deletions. Meyer MB, Benkusky NA, Pike JW. J Biol Chem. 2015 Apr 24;290(17):11093-107. doi: 10.1074/jbc.M115.648394. Epub 2015 Mar 15. PubMed
Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Maruyama T, Dougan SK, Truttmann MC, Bilate AM, Ingram JR, Ploegh HL. Nat Biotechnol. 2015 May;33(5):538-42. doi: 10.1038/nbt.3190. Epub 2015 Mar 23. PubMed
Convergence of developmental and oncogenic signaling pathways at transcriptional super-enhancers. Hnisz D, Schuijers J, Lin CY, Weintraub AS, Abraham BJ, Lee TI, Bradner JE, Young RA. Mol Cell. 2015 Apr 16;58(2):362-70. doi: 10.1016/j.molcel.2015.02.014. Epub 2015 Mar 19. PubMed
Large genomic fragment deletions and insertions in mouse using CRISPR/Cas9. Zhang L, Jia R, Palange NJ, Satheka AC, Togo J, An Y, Humphrey M, Ban L, Ji Y, Jin H, Feng X, Zheng Y. PLoS One. 2015 Mar 24;10(3):e0120396. doi: 10.1371/journal.pone.0120396. eCollection 2015. PubMed
CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. Biotechniques. 2015 Apr 1;58(4):161-70. doi: 10.2144/000114261. eCollection 2015 Apr. PubMed
TRAF Family Member-Associated NF-kappaB Activator Inhibits Genotoxic NF-kappaB Activation by Facilitating USP10-Dependent Deubiquitination of TRAF6. Wang W, Huang X, Xin HB, Fu M, Xue A, Wu ZH. J Biol Chem. 2015 Apr 10. pii: jbc.M115.643767. PubMed
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Liang P, Xu Y, Zhang X, Ding C, Huang R, Zhang Z, Lv J, Xie X, Chen Y, Li Y, Sun Y, Bai Y, Songyang Z, Ma W, Zhou C, Huang J. Protein Cell. 2015 May;6(5):363-72. doi: 10.1007/s13238-015-0153-5. Epub 2015 Apr 18. PubMed
Integrative Analysis of CRISPR/Cas9 Target Sites in the Human HBB Gene. Luo Y, Zhu D, Zhang Z, Chen Y, Sun X. Biomed Res Int. 2015;2015:514709. doi: 10.1155/2015/514709. Epub 2015 Mar 31. PubMed
Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice. Aida T, Chiyo K, Usami T, Ishikubo H, Imahashi R, Wada Y, Tanaka KF, Sakuma T, Yamamoto T, Tanaka K. Genome Biol. 2015 Apr 29;16:87. doi: 10.1186/s13059-015-0653-x. PubMed
A Rapid Embryonic Stem Cell-Based Mouse Model for B-cell Lymphomas Driven by Epstein-Barr Virus Protein LMP1. Ba Z, Meng FL, Gostissa M, Huang PY, Ke Q, Wang Z, Dao MN, Fujiwara Y, Rajewsky K, Zhang B, Alt FW. Cancer Immunol Res. 2015 Jun;3(6):641-9. doi: 10.1158/2326-6066.CIR-15-0058. Epub 2015 May 1. PubMed
Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus. Merkle FT, Neuhausser WM, Santos D, Valen E, Gagnon JA, Maas K, Sandoe J, Schier AF, Eggan K. Cell Rep. 2015 May 12;11(6):875-83. doi: 10.1016/j.celrep.2015.04.007. Epub 2015 Apr 30. PubMed
DNA Sequence-Specific Binding of CENP-B Enhances the Fidelity of Human Centromere Function. Fachinetti D, Han JS, McMahon MA, Ly P, Abdullah A, Wong AJ, Cleveland DW. Dev Cell. 2015 May 4;33(3):314-27. doi: 10.1016/j.devcel.2015.03.020. PubMed
Advantages of using the CRISPR/Cas9 system of genome editing to investigate male reproductive mechanisms using mouse models. Young SA, Aitken RJ, Ikawa M. Asian J Androl. 2015 Jul-Aug;17(4):623-7. doi: 10.4103/1008-682X.153851. PubMed
Foxp1 regulates cortical radial migration and neuronal morphogenesis in developing cerebral cortex. Li X, Xiao J, Frohlich H, Tu X, Li L, Xu Y, Cao H, Qu J, Rappold GA, Chen JG. PLoS One. 2015 May 26;10(5):e0127671. doi: 10.1371/journal.pone.0127671. eCollection 2015. PubMed
CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells. Kimura Y, Oda M, Nakatani T, Sekita Y, Monfort A, Wutz A, Mochizuki H, Nakano T. Sci Rep. 2015 Jun 3;5:10710. doi: 10.1038/srep10710. PubMed
Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems. Bialk P, Rivera-Torres N, Strouse B, Kmiec EB. PLoS One. 2015 Jun 8;10(6):e0129308. doi: 10.1371/journal.pone.0129308. eCollection 2015. PubMed
Photoactivatable CRISPR-Cas9 for optogenetic genome editing. Nihongaki Y, Kawano F, Nakajima T, Sato M. Nat Biotechnol. 2015 Jul;33(7):755-60. doi: 10.1038/nbt.3245. Epub 2015 Jun 15. PubMed
Biological Roles of Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1), HSD11B2, and Glucocorticoid Receptor (NR3C1) in Sheep Conceptus Elongation. Brooks K, Burns G, Spencer TE. Biol Reprod. 2015 Aug;93(2):38. doi: 10.1095/biolreprod.115.130757. Epub 2015 Jun 17. PubMed
Transcription Factor ATF4 Induces NLRP1 Inflammasome Expression during Endoplasmic Reticulum Stress. D'Osualdo A, Anania VG, Yu K, Lill JR, Kaufman RJ, Matsuzawa S, Reed JC. PLoS One. 2015 Jun 18;10(6):e0130635. doi: 10.1371/journal.pone.0130635. eCollection 2015. PubMed
CRISPR-mediated genotypic and phenotypic correction of a chronic granulomatous disease mutation in human iPS cells. Flynn R, Grundmann A, Renz P, Hanseler W, James WS, Cowley SA, Moore MD. Exp Hematol. 2015 Oct;43(10):838-848.e3. doi: 10.1016/j.exphem.2015.06.002. Epub 2015 Jun 19. PubMed
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Hendel A, Bak RO, Clark JT, Kennedy AB, Ryan DE, Roy S, Steinfeld I, Lunstad BD, Kaiser RJ, Wilkens AB, Bacchetta R, Tsalenko A, Dellinger D, Bruhn L, Porteus MH. Nat Biotechnol. 2015 Sep;33(9):985-9. doi: 10.1038/nbt.3290. Epub 2015 Jun 29. PubMed
An autoinhibitory mechanism modulates MAVS activity in antiviral innate immune response. Shi Y, Yuan B, Qi N, Zhu W, Su J, Li X, Qi P, Zhang D, Hou F. Nat Commun. 2015 Jul 17;6:7811. doi: 10.1038/ncomms8811. PubMed
Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells. Monfort A, Di Minin G, Postlmayr A, Freimann R, Arieti F, Thore S, Wutz A. Cell Rep. 2015 Jul 28;12(4):554-61. doi: 10.1016/j.celrep.2015.06.067. Epub 2015 Jul 16. PubMed
A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death. Ma H, Dang Y, Wu Y, Jia G, Anaya E, Zhang J, Abraham S, Choi JG, Shi G, Qi L, Manjunath N, Wu H. Cell Rep. 2015 Jul 28;12(4):673-83. doi: 10.1016/j.celrep.2015.06.049. Epub 2015 Jul 16. PubMed

Associated Plasmids

Pathogenesis of ELANE-mutant severe neutropenia revealed by induced pluripotent stem cells. Nayak RC, Trump LR, Aronow BJ, Myers K, Mehta P, Kalfa T, Wellendorf AM, Valencia CA, Paddison PJ, Horwitz MS, Grimes HL, Lutzko C, Cancelas JA. J Clin Invest. 2015 Aug 3;125(8):3103-16. doi: 10.1172/JCI80924. Epub 2015 Jul 20. PubMed
Double strand break repair by capture of retrotransposon sequences and reverse-transcribed spliced mRNA sequences in mouse zygotes. Ono R, Ishii M, Fujihara Y, Kitazawa M, Usami T, Kaneko-Ishino T, Kanno J, Ikawa M, Ishino F. Sci Rep. 2015 Jul 28;5:12281. doi: 10.1038/srep12281. PubMed
CFTR inactivation by lentiviral vector-mediated RNA interference and CRISPR-Cas9 genome editing in human airway epithelial cells. Bellec J, Bacchetta M, Losa D, Anegon I, Chanson M, Nguyen TH. Curr Gene Ther. 2015;15(5):447-59. PubMed
Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs. Wang X, Zhou J, Cao C, Huang J, Hai T, Wang Y, Zheng Q, Zhang H, Qin G, Miao X, Wang H, Cao S, Zhou Q, Zhao J. Sci Rep. 2015 Aug 21;5:13348. doi: 10.1038/srep13348. PubMed

Associated Plasmids

Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes. Crispo M, Mulet AP, Tesson L, Barrera N, Cuadro F, dos Santos-Neto PC, Nguyen TH, Creneguy A, Brusselle L, Anegon I, Menchaca A. PLoS One. 2015 Aug 25;10(8):e0136690. doi: 10.1371/journal.pone.0136690. eCollection 2015. PubMed
Orientation-specific joining of AID-initiated DNA breaks promotes antibody class switching. Dong J, Panchakshari RA, Zhang T, Zhang Y, Hu J, Volpi SA, Meyers RM, Ho YJ, Du Z, Robbiani DF, Meng F, Gostissa M, Nussenzweig MC, Manis JP, Alt FW. Nature. 2015 Sep 3;525(7567):134-9. doi: 10.1038/nature14970. Epub 2015 Aug 26. PubMed
Novel reporter system to monitor early stages of the hepatitis B virus life cycle. Nishitsuji H, Ujino S, Shimizu Y, Harada K, Zhang J, Sugiyama M, Mizokami M, Shimotohno K. Cancer Sci. 2015 Nov;106(11):1616-24. doi: 10.1111/cas.12799. Epub 2015 Oct 16. PubMed
EHMT1 and EHMT2 inhibition induces fetal hemoglobin expression. Renneville A, Van Galen P, Canver MC, McConkey M, Krill-Burger JM, Dorfman DM, Holson EB, Bernstein BE, Orkin SH, Bauer DE, Ebert BL. Blood. 2015 Oct 15;126(16):1930-9. doi: 10.1182/blood-2015-06-649087. Epub 2015 Aug 28. PubMed
Efficient genetic engineering of human intestinal organoids using electroporation. Fujii M, Matano M, Nanki K, Sato T. Nat Protoc. 2015 Oct;10(10):1474-85. doi: 10.1038/nprot.2015.088. Epub 2015 Sep 3. PubMed
Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation. Mizuno S, Takami K, Daitoku Y, Tanimoto Y, Dinh TT, Mizuno-Iijima S, Hasegawa Y, Takahashi S, Sugiyama F, Yagami K. Sci Rep. 2015 Sep 8;5:13632. doi: 10.1038/srep13632. PubMed
RORalpha Regulates Multiple Aspects of Dendrite Development in Cerebellar Purkinje Cells In Vivo. Takeo YH, Kakegawa W, Miura E, Yuzaki M. J Neurosci. 2015 Sep 9;35(36):12518-34. doi: 10.1523/JNEUROSCI.0075-15.2015. PubMed
Molecular basis underlying resistance to Mps1/TTK inhibitors. Koch A, Maia A, Janssen A, Medema RH. Oncogene. 2015 Sep 14. doi: 10.1038/onc.2015.319. PubMed
BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE. Nature. 2015 Sep 16. doi: 10.1038/nature15521. PubMed

Associated Plasmids

Tracking and transforming neocortical progenitors by CRISPR/Cas9 gene targeting and piggyBac transposase lineage labeling. Chen F, Rosiene J, Che A, Becker A, LoTurco J. Development. 2015 Oct 15;142(20):3601-11. doi: 10.1242/dev.118836. Epub 2015 Sep 23. PubMed
HIV-1 Nef promotes infection by excluding SERINC5 from virion incorporation. Rosa A, Chande A, Ziglio S, De Sanctis V, Bertorelli R, Goh SL, McCauley SM, Nowosielska A, Antonarakis SE, Luban J, Santoni FA, Pizzato M. Nature. 2015 Oct 8;526(7572):212-7. doi: 10.1038/nature15399. Epub 2015 Sep 30. PubMed
The lethal response to Cdk1 inhibition depends on sister chromatid alignment errors generated by KIF4 and isoform 1 of PRC1. Voets E, Marsman J, Demmers J, Beijersbergen R, Wolthuis R. Sci Rep. 2015 Oct 1;5:14798. doi: 10.1038/srep14798. PubMed
gp78 functions downstream of Hrd1 to promote degradation of misfolded proteins of the endoplasmic reticulum. Zhang T, Xu Y, Liu Y, Ye Y. Mol Biol Cell. 2015 Dec 1;26(24):4438-50. doi: 10.1091/mbc.E15-06-0354. Epub 2015 Sep 30. PubMed
Nuclear domain 'knock-in' screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing. Pinder J, Salsman J, Dellaire G. Nucleic Acids Res. 2015 Oct 30;43(19):9379-92. doi: 10.1093/nar/gkv993. Epub 2015 Oct 1. PubMed

Associated Plasmids

Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease. Dahlman JE, Abudayyeh OO, Joung J, Gootenberg JS, Zhang F, Konermann S. Nat Biotechnol. 2015 Oct 5;33(11):1159-1161. doi: 10.1038/nbt.3390. PubMed
CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis. Young SA, Miyata H, Satouh Y, Kato H, Nozawa K, Isotani A, Aitken RJ, Baker MA, Ikawa M. Int J Mol Sci. 2015 Oct 16;16(10):24732-50. doi: 10.3390/ijms161024732. PubMed
Identification of a novel cis-regulatory element essential for immune tolerance. LaFlam TN, Seumois G, Miller CN, Lwin W, Fasano KJ, Waterfield M, Proekt I, Vijayanand P, Anderson MS. J Exp Med. 2015 Nov 16;212(12):1993-2002. doi: 10.1084/jem.20151069. Epub 2015 Nov 2. PubMed
MITF and c-Jun antagonism interconnects melanoma dedifferentiation with pro-inflammatory cytokine responsiveness and myeloid cell recruitment. Riesenberg S, Groetchen A, Siddaway R, Bald T, Reinhardt J, Smorra D, Kohlmeyer J, Renn M, Phung B, Aymans P, Schmidt T, Hornung V, Davidson I, Goding CR, Jonsson G, Landsberg J, Tuting T, Holzel M. Nat Commun. 2015 Nov 4;6:8755. doi: 10.1038/ncomms9755. PubMed
IRE1alpha is an endogenous substrate of endoplasmic-reticulum-associated degradation. Sun S, Shi G, Sha H, Ji Y, Han X, Shu X, Ma H, Inoue T, Gao B, Kim H, Bu P, Guber RD, Shen X, Lee AH, Iwawaki T, Paton AW, Paton JC, Fang D, Tsai B, Yates JR 3rd, Wu H, Kersten S, Long Q, Duhamel GE, Simpson KW, Qi L. Nat Cell Biol. 2015 Dec;17(12):1546-55. doi: 10.1038/ncb3266. Epub 2015 Nov 9. PubMed
Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System. Wang K, Ouyang H, Xie Z, Yao C, Guo N, Li M, Jiao H, Pang D. Sci Rep. 2015 Nov 13;5:16623. doi: 10.1038/srep16623. PubMed
A forward genetic screen reveals novel independent regulators of ULBP1, an activating ligand for natural killer cells. Gowen BG, Chim B, Marceau CD, Greene TT, Burr P, Gonzalez JR, Hesser CR, Dietzen PA, Russell T, Iannello A, Coscoy L, Sentman CL, Carette JE, Muljo SA, Raulet DH. Elife. 2015 Nov 13;4. pii: e08474. doi: 10.7554/eLife.08474. PubMed
Involvement of FKBP6 in hepatitis C virus replication. Kasai H, Kawakami K, Yokoe H, Yoshimura K, Matsuda M, Yasumoto J, Maekawa S, Yamashita A, Tanaka T, Ikeda M, Kato N, Okamoto T, Matsuura Y, Sakamoto N, Enomoto N, Takeda S, Fujii H, Tsubuki M, Kusunoki M, Moriishi K. Sci Rep. 2015 Nov 16;5:16699. doi: 10.1038/srep16699. PubMed
Definitive localization of intracellular proteins: Novel approach using CRISPR-Cas9 genome editing, with glucose 6-phosphate dehydrogenase as a model. Spencer NY, Yan Z, Cong L, Zhang Y, Engelhardt JF, Stanton RC. Anal Biochem. 2016 Feb 1;494:55-67. doi: 10.1016/j.ab.2015.11.002. Epub 2015 Nov 11. PubMed
Depletion of elongation initiation factor 4E binding proteins by CRISPR/Cas9 enhances the antiviral response in porcine cells. Ramirez-Carvajal L, Singh N, de Los Santos T, Rodriguez LL, Long CR. Antiviral Res. 2016 Jan;125:8-13. doi: 10.1016/j.antiviral.2015.11.002. Epub 2015 Nov 22. PubMed
CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. Li M, Huang R, Jiang X, Chen Y, Zhang Z, Zhang X, Liang P, Zhan S, Cao S, Songyang Z, Huang J. PLoS One. 2015 Nov 24;10(11):e0143148. doi: 10.1371/journal.pone.0143148. eCollection 2015. PubMed
Large genomic fragment deletion and functional gene cassette knock-in via Cas9 protein mediated genome editing in one-cell rodent embryos. Wang L, Shao Y, Guan Y, Li L, Wu L, Chen F, Liu M, Chen H, Ma Y, Ma X, Liu M, Li D. Sci Rep. 2015 Dec 1;5:17517. doi: 10.1038/srep17517. PubMed
Production of functional small interfering RNAs by an amino-terminal deletion mutant of human Dicer. Kennedy EM, Whisnant AW, Kornepati AV, Marshall JB, Bogerd HP, Cullen BR. Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):E6945-54. doi: 10.1073/pnas.1513421112. Epub 2015 Nov 30. PubMed
The experimental power of FR900359 to study Gq-regulated biological processes. Schrage R, Schmitz AL, Gaffal E, Annala S, Kehraus S, Wenzel D, Bullesbach KM, Bald T, Inoue A, Shinjo Y, Galandrin S, Shridhar N, Hesse M, Grundmann M, Merten N, Charpentier TH, Martz M, Butcher AJ, Slodczyk T, Armando S, Effern M, Namkung Y, Jenkins L, Horn V, Stossel A, Dargatz H, Tietze D, Imhof D, Gales C, Drewke C, Muller CE, Holzel M, Milligan G, Tobin AB, Gomeza J, Dohlman HG, Sondek J, Harden TK, Bouvier M, Laporte SA, Aoki J, Fleischmann BK, Mohr K, Konig GM, Tuting T, Kostenis E. Nat Commun. 2015 Dec 14;6:10156. doi: 10.1038/ncomms10156. PubMed
Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Dang Y, Jia G, Choi J, Ma H, Anaya E, Ye C, Shankar P, Wu H. Genome Biol. 2015 Dec 15;16:280. doi: 10.1186/s13059-015-0846-3. PubMed
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Sakuma T, Nakade S, Sakane Y, Suzuki KT, Yamamoto T. Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17. PubMed

Associated Plasmids

Quantification of Protein Levels in Single Living Cells. Lo CA, Kays I, Emran F, Lin TJ, Cvetkovska V, Chen BE. Cell Rep. 2015 Dec 22;13(11):2634-44. doi: 10.1016/j.celrep.2015.11.048. Epub 2015 Dec 10. PubMed

Associated Plasmids

The bimodally expressed microRNA miR-142 gates exit from pluripotency. Sladitschek HL, Neveu PA. Mol Syst Biol. 2015 Dec 21;11(12):850. doi: 10.15252/msb.20156525. PubMed
Hepatitis C virus utilizes VLDLR as a novel entry pathway. Ujino S, Nishitsuji H, Hishiki T, Sugiyama K, Takaku H, Shimotohno K. Proc Natl Acad Sci U S A. 2016 Jan 5;113(1):188-93. doi: 10.1073/pnas.1506524113. Epub 2015 Dec 23. PubMed
The splicing regulator PTBP1 controls the activity of the transcription factor Pbx1 during neuronal differentiation. Linares AJ, Lin CH, Damianov A, Adams KL, Novitch BG, Black DL. Elife. 2015 Dec 24;4. pii: e09268. doi: 10.7554/eLife.09268. PubMed
A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes. Nakao H, Harada T, Nakao K, Kiyonari H, Inoue K, Furuta Y, Aiba A. Genesis. 2015 Dec 29. doi: 10.1002/dvg.22914. PubMed
A mouse model for adult cardiac-specific gene deletion with CRISPR/Cas9. Carroll KJ, Makarewich CA, McAnally J, Anderson DM, Zentilin L, Liu N, Giacca M, Bassel-Duby R, Olson EN. Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):338-43. doi: 10.1073/pnas.1523918113. Epub 2015 Dec 30. PubMed
Efficient CRISPR/Cas9-Based Genome Engineering in Human Pluripotent Stem Cells. Kime C, Mandegar MA, Srivastava D, Yamanaka S, Conklin BR, Rand TA. Curr Protoc Hum Genet. 2016 Jan 1;88:21.4.1-21.4.23. doi: 10.1002/0471142905.hg2104s88. PubMed
Characterization of vitamin K-dependent carboxylase mutations that cause bleeding and non-bleeding disorders. Tie JK, Carneiro JD, Jin DY, Martinhago CD, Vermeer C, Stafford DW. Blood. 2016 Jan 12. pii: blood-2015-10-677633. PubMed
In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining. Geisinger JM, Turan S, Hernandez S, Spector LP, Calos MP. Nucleic Acids Res. 2016 Jan 13. pii: gkv1542. PubMed

Associated Plasmids

Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes. Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P, Rajewsky K, Kuhn R. BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4. PubMed

Associated Plasmids

Comparison of surrogate reporter systems for enrichment of cells with mutations induced by genome editors. He Z, Shi X, Liu M, Sun G, Proudfoot C, Whitelaw CB, Lillico SG, Chen Y. J Biotechnol. 2016 Jan 14;221:49-54. doi: 10.1016/j.jbiotec.2016.01.009. PubMed
The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells. Lee CM, Cradick TJ, Bao G. Mol Ther. 2016 Mar;24(3):645-54. doi: 10.1038/mt.2016.8. Epub 2016 Jan 19. PubMed
Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining. Lu G, Duan J, Shu S, Wang X, Gao L, Guo J, Zhang Y. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1256-60. doi: 10.1073/pnas.1521597113. Epub 2016 Jan 19. PubMed
Hepatocyte factor JMJD5 regulates HBV replication through interaction with HBx. Kouwaki T, Okamoto T, Ito A, Sugiyama Y, Yamashita K, Suzuki T, Kusakabe S, Hirano J, Fukuhara T, Yamashita A, Saito K, Okuzaki D, Watashi K, Sugiyama M, Yoshio S, Standley DM, Kanto T, Mizokami M, Moriishi K, Matsuura Y. J Virol. 2016 Jan 20. pii: JVI.02776-15. PubMed
Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system. Liu X, Homma A, Sayadi J, Yang S, Ohashi J, Takumi T. Sci Rep. 2016 Jan 27;6:19675. doi: 10.1038/srep19675. PubMed
A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening. Diao Y, Li B, Meng Z, Jung I, Lee AY, Dixon J, Maliskova L, Guan KL, Shen Y, Ren B. Genome Res. 2016 Mar;26(3):397-405. doi: 10.1101/gr.197152.115. Epub 2016 Jan 26. PubMed
An essential receptor for adeno-associated virus infection. Pillay S, Meyer NL, Puschnik AS, Davulcu O, Diep J, Ishikawa Y, Jae LT, Wosen JE, Nagamine CM, Chapman MS, Carette JE. Nature. 2016 Feb 4;530(7588):108-12. doi: 10.1038/nature16465. Epub 2016 Jan 27. PubMed
Selectable one-step PCR-mediated integration of a degron for rapid depletion of endogenous human proteins. Sheridan RM, Bentley DL. Biotechniques. 2016 Feb 1;60(2):69-74. doi: 10.2144/000114378. eCollection 2016 Feb. PubMed

Associated Plasmids

A Burden of Rare Variants Associated with Extremes of Gene Expression in Human Peripheral Blood. Zhao J, Akinsanmi I, Arafat D, Cradick TJ, Lee CM, Banskota S, Marigorta UM, Bao G, Gibson G. Am J Hum Genet. 2016 Feb 4;98(2):299-309. doi: 10.1016/j.ajhg.2015.12.023. PubMed
A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs. Romanienko PJ, Giacalone J, Ingenito J, Wang Y, Isaka M, Johnson T, You Y, Mark WH. PLoS One. 2016 Feb 5;11(2):e0148362. doi: 10.1371/journal.pone.0148362. eCollection 2016. PubMed

Associated Plasmids

Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair. Gutschner T, Haemmerle M, Genovese G, Draetta GF, Chin L. Cell Rep. 2016 Feb 3. pii: S2211-1247(16)00040-1. doi: 10.1016/j.celrep.2016.01.019. PubMed

Associated Plasmids

Targeted Gene Deletion Using DNA-Free RNA-Guided Cas9 Nuclease Accelerates Adaptation of CHO Cells to Suspension Culture. Lee N, Shin J, Park JH, Lee GM, Cho S, Cho BK. ACS Synth Biol. 2016 Nov 18;5(11):1211-1219. Epub 2016 Feb 18. PubMed
CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation. Shinmyo Y, Tanaka S, Tsunoda S, Hosomichi K, Tajima A, Kawasaki H. Sci Rep. 2016 Feb 9;6:20611. doi: 10.1038/srep20611. PubMed
Structure-function analysis of myomaker domains required for myoblast fusion. Millay DP, Gamage DG, Quinn ME, Min YL, Mitani Y, Bassel-Duby R, Olson EN. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):2116-21. doi: 10.1073/pnas.1600101113. Epub 2016 Feb 8. PubMed
Immunomodulatory drugs inhibit TLR4-induced type-1 interferon production independently of Cereblon via suppression of the TRIF/IRF3 pathway. Millrine D, Miyata H, Tei M, Dubey P, Nyati K, Nakahama T, Gemechu Y, Ripley B, Kishimoto T. Int Immunol. 2016 Jun;28(6):307-15. doi: 10.1093/intimm/dxw005. Epub 2016 Feb 10. PubMed
Glycosphingolipids on Human Myeloid Cells Stabilize E-Selectin-Dependent Rolling in the Multistep Leukocyte Adhesion Cascade. Mondal N, Stolfa G, Antonopoulos A, Zhu Y, Wang SS, Buffone A Jr, Atilla-Gokcumen GE, Haslam SM, Dell A, Neelamegham S. Arterioscler Thromb Vasc Biol. 2016 Apr;36(4):718-27. doi: 10.1161/ATVBAHA.115.306748. Epub 2016 Feb 11. PubMed
Transcription-associated processes cause DNA double-strand breaks and translocations in neural stem/progenitor cells. Schwer B, Wei PC, Chang AN, Kao J, Du Z, Meyers RM, Alt FW. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):2258-63. doi: 10.1073/pnas.1525564113. Epub 2016 Feb 12. PubMed
Highly Efficient CRISPR/Cas9-Mediated Cloning and Functional Characterization of Gastric Cancer-Derived Epstein-Barr Virus Strains. Kanda T, Furuse Y, Oshitani H, Kiyono T. J Virol. 2016 Apr 14;90(9):4383-93. doi: 10.1128/JVI.00060-16. Print 2016 May 1. PubMed
Precise Correction of Disease Mutations in Induced Pluripotent Stem Cells Derived From Patients With Limb Girdle Muscular Dystrophy. Turan S, Farruggio AP, Srifa W, Day JW, Calos MP. Mol Ther. 2016 Apr;24(4):685-96. doi: 10.1038/mt.2016.40. Epub 2016 Feb 26. PubMed
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice. Maresch R, Mueller S, Veltkamp C, Ollinger R, Friedrich M, Heid I, Steiger K, Weber J, Engleitner T, Barenboim M, Klein S, Louzada S, Banerjee R, Strong A, Stauber T, Gross N, Geumann U, Lange S, Ringelhan M, Varela I, Unger K, Yang F, Schmid RM, Vassiliou GS, Braren R, Schneider G, Heikenwalder M, Bradley A, Saur D, Rad R. Nat Commun. 2016 Feb 26;7:10770. doi: 10.1038/ncomms10770. PubMed
A RUNX2-Mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells. Shin MH, He Y, Marrogi E, Piperdi S, Ren L, Khanna C, Gorlick R, Liu C, Huang J. PLoS Genet. 2016 Feb 29;12(2):e1005884. doi: 10.1371/journal.pgen.1005884. eCollection 2016 Feb. PubMed
Generating Mouse Models Using CRISPR-Cas9-Mediated Genome Editing. Qin W, Kutny PM, Maser RS, Dion SL, Lamont JD, Zhang Y, Perry GA, Wang H. Curr Protoc Mouse Biol. 2016 Mar 1;6(1):39-66. doi: 10.1002/9780470942390.mo150178. PubMed
Structural and Functional Analysis of a Novel Interaction Motif within UFM1-activating Enzyme 5 (UBA5) Required for Binding to Ubiquitin-like Proteins and Ufmylation. Habisov S, Huber J, Ichimura Y, Akutsu M, Rogova N, Loehr F, McEwan DG, Johansen T, Dikic I, Doetsch V, Komatsu M, Rogov VV, Kirkin V. J Biol Chem. 2016 Apr 22;291(17):9025-41. doi: 10.1074/jbc.M116.715474. Epub 2016 Feb 29. PubMed
ECEL1 mutation implicates impaired axonal arborization of motor nerves in the pathogenesis of distal arthrogryposis. Nagata K, Kiryu-Seo S, Tamada H, Okuyama-Uchimura F, Kiyama H, Saido TC. Acta Neuropathol. 2016 Jul;132(1):111-26. doi: 10.1007/s00401-016-1554-0. Epub 2016 Mar 7. PubMed
Pathologic activation of thrombopoietin receptor and JAK2-STAT5 pathway by frameshift mutants of mouse calreticulin. Balligand T, Achouri Y, Pecquet C, Chachoua I, Nivarthi H, Marty C, Vainchenker W, Plo I, Kralovics R, Constantinescu SN. Leukemia. 2016 Feb 29. doi: 10.1038/leu.2016.47. PubMed
Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/beta-catenin defects in neuronal cholesterol synthesis phenotypes. Francis KR, Ton AN, Xin Y, O'Halloran PE, Wassif CA, Malik N, Williams IM, Cluzeau CV, Trivedi NS, Pavan WJ, Cho W, Westphal H, Porter FD. Nat Med. 2016 Apr;22(4):388-96. doi: 10.1038/nm.4067. Epub 2016 Mar 21. PubMed
Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection. Estep JA, Sternburg EL, Sanchez GA, Karginov FV. BMC Mol Biol. 2016 Apr 2;17:9. doi: 10.1186/s12867-016-0061-0. PubMed
Unlinking an lncRNA from Its Associated cis Element. Paralkar VR, Taborda CC, Huang P, Yao Y, Kossenkov AV, Prasad R, Luan J, Davies JO, Hughes JR, Hardison RC, Blobel GA, Weiss MJ. Mol Cell. 2016 Apr 7;62(1):104-10. doi: 10.1016/j.molcel.2016.02.029. Epub 2016 Mar 31. PubMed
Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT. Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24. PubMed

Associated Plasmids

Lentivirus pre-packed with Cas9 protein for safer gene editing. Choi JG, Dang Y, Abraham S, Ma H, Zhang J, Guo H, Cai Y, Mikkelsen JG, Wu H, Shankar P, Manjunath N. Gene Ther. 2016 Jul;23(7):627-33. doi: 10.1038/gt.2016.27. Epub 2016 Apr 7. PubMed
Generation of CRISPR/Cas9-mediated bicistronic knock-in Ins1-cre driver mice. Hasegawa Y, Hoshino Y, Ibrahim AE, Kato K, Daitoku Y, Tanimoto Y, Ikeda Y, Oishi H, Takahashi S, Yoshiki A, Yagami KI, Iseki H, Mizuno S, Sugiyama F. Exp Anim. 2016 Apr 7. PubMed
Cleavage by Caspase 8 and Mitochondrial Membrane Association Activate the BH3-only Protein Bid during TRAIL-induced Apoptosis. Huang K, Zhang J, O'Neill KL, Gurumurthy CB, Quadros RM, Tu Y, Luo X. J Biol Chem. 2016 May 27;291(22):11843-51. doi: 10.1074/jbc.M115.711051. Epub 2016 Apr 6. PubMed
The mitochondrial outer membrane protein MDI promotes local protein synthesis and mtDNA replication. Zhang Y, Chen Y, Gucek M, Xu H. EMBO J. 2016 May 17;35(10):1045-57. doi: 10.15252/embj.201592994. Epub 2016 Apr 6. PubMed
Inactivation of prosurvival Bcl-2 proteins activates Bax/Bak through the outer mitochondrial membrane. O'Neill KL, Huang K, Zhang J, Chen Y, Luo X. Genes Dev. 2016 Apr 15;30(8):973-88. doi: 10.1101/gad.276725.115. Epub 2016 Apr 7. PubMed
A Genome-wide CRISPR Screen Identifies CDC25A as a Determinant of Sensitivity to ATR Inhibitors. Ruiz S, Mayor-Ruiz C, Lafarga V, Murga M, Vega-Sendino M, Ortega S, Fernandez-Capetillo O. Mol Cell. 2016 Apr 21;62(2):307-313. doi: 10.1016/j.molcel.2016.03.006. Epub 2016 Apr 7. PubMed
Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells. Meininger I, Griesbach RA, Hu D, Gehring T, Seeholzer T, Bertossi A, Kranich J, Oeckinghaus A, Eitelhuber AC, Greczmiel U, Gewies A, Schmidt-Supprian M, Ruland J, Brocker T, Heissmeyer V, Heyd F, Krappmann D. Nat Commun. 2016 Apr 12;7:11292. doi: 10.1038/ncomms11292. PubMed
Effects of pharmacological and genetic disruption of CXCR4 chemokine receptor function in B-cell acute lymphoblastic leukaemia. Randhawa S, Cho BS, Ghosh D, Sivina M, Koehrer S, Muschen M, Peled A, Davis RE, Konopleva M, Burger JA. Br J Haematol. 2016 Apr 12. doi: 10.1111/bjh.14075. PubMed
Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli. Bassalo MC, Garst AD, Halweg-Edwards AL, Grau WC, Domaille DW, Mutalik VK, Arkin AP, Gill RT. ACS Synth Biol. 2016 Apr 22. PubMed

Associated Plasmids

EZH2-mediated repression of GSK-3beta and TP53 promotes Wnt/beta-catenin signaling-dependent cell expansion in cervical carcinoma. Chen Q, Zheng PS, Yang WT. Oncotarget. 2016 Jun 14;7(24):36115-36129. doi: 10.18632/oncotarget.8741. PubMed
A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular Research. Miano JM, Zhu QM, Lowenstein CJ. Arterioscler Thromb Vasc Biol. 2016 Jun;36(6):1058-75. doi: 10.1161/ATVBAHA.116.304790. Epub 2016 Apr 21. PubMed
Generation of Human Embryonic Stem Cell Line Expressing zsGreen in Cholinergic Neurons Using CRISPR/Cas9 System. Zhou J, Wang C, Zhang K, Wang Y, Gong X, Wang Y, Li S, Luo Y. Neurochem Res. 2016 Apr 25. PubMed
Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9. Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS. PLoS One. 2016 Apr 29;11(4):e0154604. doi: 10.1371/journal.pone.0154604. eCollection 2016. PubMed

Associated Plasmids

TPC2 controls pigmentation by regulating melanosome pH and size. Ambrosio AL, Boyle JA, Aradi AE, Christian KA, Di Pietro SM. Proc Natl Acad Sci U S A. 2016 May 17;113(20):5622-7. doi: 10.1073/pnas.1600108113. Epub 2016 May 2. PubMed

Associated Plasmids

TRC8-dependent degradation of hepatitis C virus immature core protein regulates viral propagation and pathogenesis. Aizawa S, Okamoto T, Sugiyama Y, Kouwaki T, Ito A, Suzuki T, Ono C, Fukuhara T, Yamamoto M, Okochi M, Hiraga N, Imamura M, Chayama K, Suzuki R, Shoji I, Moriishi K, Moriya K, Koike K, Matsuura Y. Nat Commun. 2016 May 4;7:11379. doi: 10.1038/ncomms11379. PubMed
Lipoprotein Receptors Redundantly Participate in Entry of Hepatitis C Virus. Yamamoto S, Fukuhara T, Ono C, Uemura K, Kawachi Y, Shiokawa M, Mori H, Wada M, Shima R, Okamoto T, Hiraga N, Suzuki R, Chayama K, Wakita T, Matsuura Y. PLoS Pathog. 2016 May 6;12(5):e1005610. doi: 10.1371/journal.ppat.1005610. eCollection 2016 May. PubMed
Lactase nonpersistence is directed by DNA-variation-dependent epigenetic aging. Labrie V, Buske OJ, Oh E, Jeremian R, Ptak C, Gasiunas G, Maleckas A, Petereit R, Zvirbliene A, Adamonis K, Kriukiene E, Koncevicius K, Gordevicius J, Nair A, Zhang A, Ebrahimi S, Oh G, Siksnys V, Kupcinskas L, Brudno M, Petronis A. Nat Struct Mol Biol. 2016 Jun;23(6):566-73. doi: 10.1038/nsmb.3227. Epub 2016 May 9. PubMed
The SAGA Deubiquitination Module Promotes DNA Repair and Class Switch Recombination through ATM and DNAPK-Mediated gammaH2AX Formation. Ramachandran S, Haddad D, Li C, Le MX, Ling AK, So CC, Nepal RM, Gommerman JL, Yu K, Ketela T, Moffat J, Martin A. Cell Rep. 2016 May 17;15(7):1554-65. doi: 10.1016/j.celrep.2016.04.041. Epub 2016 May 5. PubMed
High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing. Mikuni T, Nishiyama J, Sun Y, Kamasawa N, Yasuda R. Cell. 2016 Jun 16;165(7):1803-17. doi: 10.1016/j.cell.2016.04.044. Epub 2016 May 12. PubMed
Mesp1 controls the speed, polarity, and directionality of cardiovascular progenitor migration. Chiapparo G, Lin X, Lescroart F, Chabab S, Paulissen C, Pitisci L, Bondue A, Blanpain C. J Cell Biol. 2016 May 23;213(4):463-77. doi: 10.1083/jcb.201505082. Epub 2016 May 16. PubMed
A nonsense mutation in the DNA repair factor Hebo causes mild bone marrow failure and microcephaly. Zhang S, Pondarre C, Pennarun G, Labussiere-Wallet H, Vera G, France B, Chansel M, Rouvet I, Revy P, Lopez B, Soulier J, Bertrand P, Callebaut I, de Villartay JP. J Exp Med. 2016 May 30;213(6):1011-28. doi: 10.1084/jem.20151183. Epub 2016 May 16. PubMed
Spermatogenic Cell-Specific Gene Mutation in Mice via CRISPR-Cas9. Bai M, Liang D, Wang Y, Li Q, Wu Y, Li J. J Genet Genomics. 2016 May 20;43(5):289-96. doi: 10.1016/j.jgg.2016.02.003. Epub 2016 Feb 26. PubMed
NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met). Nakano S, Suzuki T, Kawarada L, Iwata H, Asano K, Suzuki T. Nat Chem Biol. 2016 Jul;12(7):546-51. doi: 10.1038/nchembio.2099. Epub 2016 May 23. PubMed
Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders. Shigeta M, Sakane Y, Iida M, Suzuki M, Kashiwagi K, Kashiwagi A, Fujii S, Yamamoto T, Suzuki KT. Genes Cells. 2016 Jul;21(7):755-71. doi: 10.1111/gtc.12379. Epub 2016 May 24. PubMed
Infantile Pain Episodes Associated with Novel Nav1.9 Mutations in Familial Episodic Pain Syndrome in Japanese Families. Okuda H, Noguchi A, Kobayashi H, Kondo D, Harada KH, Youssefian S, Shioi H, Kabata R, Domon Y, Kubota K, Kitano Y, Takayama Y, Hitomi T, Ohno K, Saito Y, Asano T, Tominaga M, Takahashi T, Koizumi A. PLoS One. 2016 May 25;11(5):e0154827. doi: 10.1371/journal.pone.0154827. eCollection 2016. PubMed
Generation of a Double KO Mouse by Simultaneous Targeting of the Neighboring Genes Tmem176a and Tmem176b Using CRISPR/Cas9: Key Steps from Design to Genotyping. Lemoine A, Chauveau-Le Friec G, Langa F, Louvet C. J Genet Genomics. 2016 May 20;43(5):329-40. doi: 10.1016/j.jgg.2016.04.004. Epub 2016 Apr 22. PubMed
Functional validation of tensin2 SH2-PTB domain by CRISPR/Cas9-mediated genome editing. Marusugi K, Nakano K, Sasaki H, Kimura J, Yanobu-Takanashi R, Okamura T, Sasaki N. J Vet Med Sci. 2016 May 30. PubMed
Hyperlipidemia and hepatitis in liver-specific CREB3L3 knockout mice generated using a one-step CRISPR/Cas9 system. Nakagawa Y, Oikawa F, Mizuno S, Ohno H, Yagishita Y, Satoh A, Osaki Y, Takei K, Kikuchi T, Han SI, Matsuzaka T, Iwasaki H, Kobayashi K, Yatoh S, Yahagi N, Isaka M, Suzuki H, Sone H, Takahashi S, Yamada N, Shimano H. Sci Rep. 2016 Jun 13;6:27857. doi: 10.1038/srep27857. PubMed
Unconventional secretion of misfolded proteins promotes adaptation to proteasome dysfunction in mammalian cells. Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y. Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 2016 Jun 13. PubMed

Associated Plasmids

Constitutive Activation of PINK1 Protein Leads to Proteasome-mediated and Non-apoptotic Cell Death Independently of Mitochondrial Autophagy. Akabane S, Matsuzaki K, Yamashita S, Arai K, Okatsu K, Kanki T, Matsuda N, Oka T. J Biol Chem. 2016 Jul 29;291(31):16162-74. doi: 10.1074/jbc.M116.714923. Epub 2016 Jun 14. PubMed
Bax/Bak activation in the absence of Bid, Bim, Puma, and p53. Zhang J, Huang K, O'Neill KL, Pang X, Luo X. Cell Death Dis. 2016 Jun 16;7:e2266. doi: 10.1038/cddis.2016.167. PubMed
Inactivation of the CRL4-CDT2-SET8/p21 ubiquitylation and degradation axis underlies the therapeutic efficacy of pevonedistat in melanoma. Benamar M, Guessous F, Du K, Corbett P, Obeid J, Gioeli D, Slingluff CL Jr, Abbas T. EBioMedicine. 2016 Jun 16. pii: S2352-3964(16)30279-1. doi: 10.1016/j.ebiom.2016.06.023. PubMed
S100A4 accelerates the proliferation and invasion of endometrioid carcinoma and is associated with the "MELF" pattern. Tahara S, Nojima S, Ohshima K, Hori Y, Kurashige M, Wada N, Ikeda J, Morii E. Cancer Sci. 2016 Sep;107(9):1345-52. doi: 10.1111/cas.12999. Epub 2016 Aug 12. PubMed
Loss of function mutation in LOX causes thoracic aortic aneurysm and dissection in humans. Lee VS, Halabi CM, Hoffman EP, Carmichael N, Leshchiner I, Lian CG, Bierhals AJ, Vuzman D, Mecham RP, Frank NY, Stitziel NO. Proc Natl Acad Sci U S A. 2016 Aug 2;113(31):8759-64. doi: 10.1073/pnas.1601442113. Epub 2016 Jul 18. PubMed
BRCA1185delAG tumors may acquire therapy resistance through expression of RING-less BRCA1. Drost R, Dhillon KK, van der Gulden H, van der Heijden I, Brandsma I, Cruz C, Chondronasiou D, Castroviejo-Bermejo M, Boon U, Schut E, van der Burg E, Wientjens E, Pieterse M, Klijn C, Klarenbeek S, Loayza-Puch F, Elkon R, van Deemter L, Rottenberg S, van de Ven M, Dekkers DH, Demmers JA, van Gent DC, Agami R, Balmana J, Serra V, Taniguchi T, Bouwman P, Jonkers J. J Clin Invest. 2016 Aug 1;126(8):2903-18. doi: 10.1172/JCI70196. Epub 2016 Jul 25. PubMed
Co-incident insertion enables high efficiency genome engineering in mouse embryonic stem cells. Shy BR, MacDougall MS, Clarke R, Merrill BJ. Nucleic Acids Res. 2016 Aug 2. pii: gkw685. PubMed
Investigation of the functional role of human Interleukin-8 gene haplotypes by CRISPR/Cas9 mediated genome editing. Benakanakere MR, Finoti LS, Tanaka U, Grant GR, Scarel-Caminaga RM, Kinane DF. Sci Rep. 2016 Aug 8;6:31180. doi: 10.1038/srep31180. PubMed
Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres. Schmidt JC, Zaug AJ, Cech TR. Cell. 2016 Aug 25;166(5):1188-1197.e9. doi: 10.1016/j.cell.2016.07.033. Epub 2016 Aug 11. PubMed

Associated Plasmids

Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes. Richardson CD, Ray GJ, Bray NL, Corn JE. Nat Commun. 2016 Aug 17;7:12463. doi: 10.1038/ncomms12463. PubMed
CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice. Oji A, Noda T, Fujihara Y, Miyata H, Kim YJ, Muto M, Nozawa K, Matsumura T, Isotani A, Ikawa M. Sci Rep. 2016 Aug 17;6:31666. doi: 10.1038/srep31666. PubMed
Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Santos DP, Kiskinis E, Eggan K, Merkle FT. Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.6.1-5B.6.60. doi: 10.1002/cpsc.15. PubMed
The type 2 diabetes presumed causal variant within TCF7L2 resides in an element that controls the expression of ACSL5. Xia Q, Chesi A, Manduchi E, Johnston BT, Lu S, Leonard ME, Parlin UW, Rappaport EF, Huang P, Wells AD, Blobel GA, Johnson ME, Grant SF. Diabetologia. 2016 Nov;59(11):2360-8. doi: 10.1007/s00125-016-4077-2. Epub 2016 Aug 18. PubMed
53BP1 Integrates DNA Repair and p53-Dependent Cell Fate Decisions via Distinct Mechanisms. Cuella-Martin R, Oliveira C, Lockstone HE, Snellenberg S, Grolmusova N, Chapman JR. Mol Cell. 2016 Oct 6;64(1):51-64. doi: 10.1016/j.molcel.2016.08.002. Epub 2016 Aug 18. PubMed
Impaired striatal dopamine release in homozygous Vps35 D620N knock-in mice. Ishizu N, Yui D, Hebisawa A, Aizawa H, Cui W, Fujita Y, Hashimoto K, Ajioka I, Mizusawa H, Yokota T, Watase K. Hum Mol Genet. 2016 Aug 25. pii: ddw279. PubMed
Covalent targeting of remote cysteine residues to develop CDK12 and CDK13 inhibitors. Zhang T, Kwiatkowski N, Olson CM, Dixon-Clarke SE, Abraham BJ, Greifenberg AK, Ficarro SB, Elkins JM, Liang Y, Hannett NM, Manz T, Hao M, Bartkowiak B, Greenleaf AL, Marto JA, Geyer M, Bullock AN, Young RA, Gray NS. Nat Chem Biol. 2016 Oct;12(10):876-84. doi: 10.1038/nchembio.2166. Epub 2016 Aug 29. PubMed
Generation of DeltaF508-CFTR T84 cell lines by CRISPR/Cas9-mediated genome editing. Chung WY, Song M, Park J, Namkung W, Lee J, Kim H, Lee MG, Kim JY. Biotechnol Lett. 2016 Dec;38(12):2023-2034. Epub 2016 Aug 29. PubMed
Reprogramming metabolic pathways in vivo with CRISPR/Cas9 genome editing to treat hereditary tyrosinaemia. Pankowicz FP, Barzi M, Legras X, Hubert L, Mi T, Tomolonis JA, Ravishankar M, Sun Q, Yang D, Borowiak M, Sumazin P, Elsea SH, Bissig-Choisat B, Bissig KD. Nat Commun. 2016 Aug 30;7:12642. doi: 10.1038/ncomms12642. PubMed
Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome. Xie C, Zhang YP, Song L, Luo J, Qi W, Hu J, Lu D, Yang Z, Zhang J, Xiao J, Zhou B, Du JL, Jing N, Liu Y, Wang Y, Li BL, Song BL, Yan Y. Cell Res. 2016 Oct;26(10):1099-1111. doi: 10.1038/cr.2016.101. Epub 2016 Aug 30. PubMed
Long noncoding RNA #32 contributes to antiviral responses by controlling interferon-stimulated gene expression. Nishitsuji H, Ujino S, Yoshio S, Sugiyama M, Mizokami M, Kanto T, Shimotohno K. Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):10388-93. doi: 10.1073/pnas.1525022113. Epub 2016 Aug 31. PubMed
CRISPR/Cas9 Technology Targeting Fas Gene Protects Mice From Concanavalin-A Induced Fulminant Hepatic Failure. Liang WC, Liang PP, Wong CW, Ng TB, Huang JJ, Zhang JF, Waye MM, Fu WM. J Cell Biochem. 2017 Mar;118(3):530-536. doi: 10.1002/jcb.25722. Epub 2016 Oct 12. PubMed
Epithelial Expression of Human ABO Blood Group Genes Is Dependent upon a Downstream Regulatory Element Functioning through an Epithelial Cell-specific Transcription Factor, Elf5. Sano R, Nakajima T, Takahashi Y, Kubo R, Kobayashi M, Takahashi K, Takeshita H, Ogasawara K, Kominato Y. J Biol Chem. 2016 Oct 21;291(43):22594-22606. Epub 2016 Sep 1. PubMed
The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. de Solis CA, Ho A, Holehonnur R, Ploski JE. Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070. eCollection 2016. PubMed

Associated Plasmids

Sendai virus, an RNA virus with no risk of genomic integration, delivers CRISPR/Cas9 for efficient gene editing. Park A, Hong P, Won ST, Thibault PA, Vigant F, Oguntuyo KY, Taft JD, Lee B. Mol Ther Methods Clin Dev. 2016 Aug 24;3:16057. doi: 10.1038/mtm.2016.57. eCollection 2016. PubMed
Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides. Bialk P, Sansbury B, Rivera-Torres N, Bloh K, Man D, Kmiec EB. Sci Rep. 2016 Sep 9;6:32681. doi: 10.1038/srep32681. PubMed
Efficient production of biallelic GGTA1 knockout pigs by cytoplasmic microinjection of CRISPR/Cas9 into zygotes. Petersen B, Frenzel A, Lucas-Hahn A, Herrmann D, Hassel P, Klein S, Ziegler M, Hadeler KG, Niemann H. Xenotransplantation. 2016 Sep;23(5):338-46. doi: 10.1111/xen.12258. Epub 2016 Sep 9. PubMed
Perichromosomal protein Ki67 supports mitotic chromosome architecture. Takagi M, Natsume T, Kanemaki MT, Imamoto N. Genes Cells. 2016 Oct;21(10):1113-1124. doi: 10.1111/gtc.12420. Epub 2016 Sep 9. PubMed
A G-Rich Motif in the lncRNA Braveheart Interacts with a Zinc-Finger Transcription Factor to Specify the Cardiovascular Lineage. Xue Z, Hennelly S, Doyle B, Gulati AA, Novikova IV, Sanbonmatsu KY, Boyer LA. Mol Cell. 2016 Oct 6;64(1):37-50. doi: 10.1016/j.molcel.2016.08.010. Epub 2016 Sep 8. PubMed
PtdIns(4,5)P2 signaling regulates ATG14 and autophagy. Tan X, Thapa N, Liao Y, Choi S, Anderson RA. Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):10896-901. doi: 10.1073/pnas.1523145113. Epub 2016 Sep 12. PubMed
CRISPR/Cas9-mediated mutation revealed cytoplasmic tail is dispensable for IZUMO1 function and male fertility. Young SA, Miyata H, Satouh Y, Muto M, Larsen MR, Aitken RJ, Baker MA, Ikawa M. Reproduction. 2016 Dec;152(6):665-672. Epub 2016 Sep 13. PubMed
Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells. Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, Fodor E, Welker E. Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0. PubMed

Associated Plasmids

The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells. Roncagalli R, Cucchetti M, Jarmuzynski N, Gregoire C, Bergot E, Audebert S, Baudelet E, Menoita MG, Joachim A, Durand S, Suchanek M, Fiore F, Zhang L, Liang Y, Camoin L, Malissen M, Malissen B. J Exp Med. 2016 Oct 17;213(11):2437-2457. Epub 2016 Sep 19. PubMed
Tetherin is an exosomal tether. Edgar JR, Manna PT, Nishimura S, Banting G, Robinson MS. Elife. 2016 Sep 22;5. pii: e17180. doi: 10.7554/eLife.17180. PubMed
Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein. Richter-Dennerlein R, Oeljeklaus S, Lorenzi I, Ronsor C, Bareth B, Schendzielorz AB, Wang C, Warscheid B, Rehling P, Dennerlein S. Cell. 2016 Oct 6;167(2):471-483.e10. doi: 10.1016/j.cell.2016.09.003. Epub 2016 Sep 29. PubMed
Upregulation of ANGPTL6 in mouse keratinocytes enhances susceptibility to psoriasis. Tanigawa H, Miyata K, Tian Z, Aoi J, Kadomatsu T, Fukushima S, Ogata A, Takeda N, Zhao J, Zhu S, Terada K, Endo M, Morinaga J, Sugizaki T, Sato M, Morioka MS, Manabe I, Mashimo Y, Hata A, Taketomi Y, Yamamoto K, Murakami M, Araki K, Jinnin M, Ihn H, Oike Y. Sci Rep. 2016 Oct 4;6:34690. doi: 10.1038/srep34690. PubMed
MyoD Regulates Skeletal Muscle Oxidative Metabolism Cooperatively with Alternative NF-kappaB. Shintaku J, Peterson JM, Talbert EE, Gu JM, Ladner KJ, Williams DR, Mousavi K, Wang R, Sartorelli V, Guttridge DC. Cell Rep. 2016 Oct 4;17(2):514-526. doi: 10.1016/j.celrep.2016.09.010. PubMed
Evolution of Hoxa11 regulation in vertebrates is linked to the pentadactyl state. Kherdjemil Y, Lalonde RL, Sheth R, Dumouchel A, de Martino G, Pineault KM, Wellik DM, Stadler HS, Akimenko MA, Kmita M. Nature. 2016 Nov 3;539(7627):89-92. doi: 10.1038/nature19813. Epub 2016 Oct 5. PubMed
Foot-and-Mouth Disease Virus Viroporin 2B Antagonizes RIG-I-Mediated Antiviral Effects by Inhibition of Its Protein Expression. Zhu Z, Wang G, Yang F, Cao W, Mao R, Du X, Zhang X, Li C, Li D, Zhang K, Shu H, Liu X, Zheng H. J Virol. 2016 Nov 28;90(24):11106-11121. Print 2016 Dec 15. PubMed
Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells. Lee CM, Zhu H, Davis TH, Deshmukh H, Bao G. Methods Mol Biol. 2017;1498:3-21. PubMed
CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis. Naert T, Colpaert R, Van Nieuwenhuysen T, Dimitrakopoulou D, Leoen J, Haustraete J, Boel A, Steyaert W, Lepez T, Deforce D, Willaert A, Creytens D, Vleminckx K. Sci Rep. 2016 Oct 14;6:35264. doi: 10.1038/srep35264. PubMed
Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T. Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. PubMed

Associated Plasmids

The Impact of Chromatin Dynamics on Cas9-Mediated Genome Editing in Human Cells. Daer RM, Cutts JP, Brafman DA, Haynes KA. ACS Synth Biol. 2017 Mar 17;6(3):428-438. doi: 10.1021/acssynbio.5b00299. Epub 2016 Nov 17. PubMed
C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility. Gomez-H L, Felipe-Medina N, Sanchez-Martin M, Davies OR, Ramos I, Garcia-Tunon I, de Rooij DG, Dereli I, Toth A, Barbero JL, Benavente R, Llano E, Pendas AM. Nat Commun. 2016 Oct 31;7:13298. doi: 10.1038/ncomms13298. PubMed
DNA methylation-independent removable insulator controls chromatin remodeling at the HOXA locus via retinoic acid signaling. Ishihara K, Nakamoto M, Nakao M. Hum Mol Genet. 2016 Oct 24. pii: ddw354. doi: 10.1093/hmg/ddw354. PubMed
CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa. Young SA, Miyata H, Satouh Y, Aitken RJ, Baker MA, Ikawa M. J Cell Sci. 2016 Dec 1;129(23):4379-4387. Epub 2016 Oct 19. PubMed
Structural and functional consequences of a disease mutation in the telomere protein TPP1. Bisht K, Smith EM, Tesmer VM, Nandakumar J. Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):13021-13026. Epub 2016 Nov 2. PubMed
Calreticulin mutant mice develop essential thrombocythemia that is ameliorated by the JAK inhibitor ruxolitinib. Shide K, Kameda T, Yamaji T, Sekine M, Inada N, Kamiunten A, Akizuki K, Nakamura K, Hidaka T, Kubuki Y, Shimoda H, Kitanaka A, Honda A, Sawaguchi A, Abe H, Miike T, Iwakiri H, Tahara Y, Sueta M, Hasuike S, Yamamoto S, Nagata K, Shimoda K. Leukemia. 2016 Nov 29. doi: 10.1038/leu.2016.308. PubMed
RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation. Lin J, Kumari S, Kim C, Van TM, Wachsmuth L, Polykratis A, Pasparakis M. Nature. 2016 Dec 1;540(7631):124-128. doi: 10.1038/nature20558. Epub 2016 Nov 7. PubMed
Re-visiting the Protamine-2 locus: deletion, but not haploinsufficiency, renders male mice infertile. Schneider S, Balbach M, Jan F Jikeli, Fietz D, Nettersheim D, Jostes S, Schmidt R, Kressin M, Bergmann M, Wachten D, Steger K, Schorle H. Sci Rep. 2016 Nov 11;6:36764. doi: 10.1038/srep36764. PubMed
Epigenetic upregulation of ARL4C, due to DNA hypomethylation in the 3'-untranslated region, promotes tumorigenesis of lung squamous cell carcinoma. Fujii S, Shinjo K, Matsumoto S, Harada T, Nojima S, Sato S, Usami Y, Toyosawa S, Morii E, Kondo Y, Kikuchi A. Oncotarget. 2016 Dec 6;7(49):81571-81587. doi: 10.18632/oncotarget.13147. PubMed
Phospho-ubiquitin-PARK2 complex as a marker for mitophagy defects. Callegari S, Oeljeklaus S, Warscheid B, Dennerlein S, Thumm M, Rehling P, Dudek J. Autophagy. 2017 Jan 2;13(1):201-211. doi: 10.1080/15548627.2016.1254852. Epub 2016 Nov 15. PubMed
Kin17 facilitates multiple double-strand break repair pathways that govern B cell class switching. Le MX, Haddad D, Ling AK, Li C, So CC, Chopra A, Hu R, Angulo JF, Moffat J, Martin A. Sci Rep. 2016 Nov 17;6:37215. doi: 10.1038/srep37215. PubMed
pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Tsutsui H, Higashiyama T. Plant Cell Physiol. 2016 Nov 17. pii: pcw191. PubMed

Associated Plasmids

Long-Range Control of Renin Gene Expression in Tsukuba Hypertensive Mice. Ushiki A, Matsuzaki H, Ishida J, Fukamizu A, Tanimoto K. PLoS One. 2016 Nov 18;11(11):e0166974. doi: 10.1371/journal.pone.0166974. eCollection 2016. PubMed
Mouse Tmem135 mutation reveals a mechanism involving mitochondrial dynamics that leads to age-dependent retinal pathologies. Lee WH, Higuchi H, Ikeda S, Macke EL, Takimoto T, Pattnaik BR, Liu C, Chu LF, Siepka SM, Krentz KJ, Rubinstein CD, Kalejta RF, Thomson JA, Mullins RF, Takahashi JS, Pinto LH, Ikeda A. Elife. 2016 Nov 15;5. pii: e19264. doi: 10.7554/eLife.19264. PubMed
PRDM14 Drives OCT3/4 Recruitment via Active Demethylation in the Transition from Primed to Naive Pluripotency. Okashita N, Suwa Y, Nishimura O, Sakashita N, Kadota M, Nagamatsu G, Kawaguchi M, Kashida H, Nakajima A, Tachibana M, Seki Y. Stem Cell Reports. 2016 Dec 13;7(6):1072-1086. doi: 10.1016/j.stemcr.2016.10.007. Epub 2016 Nov 17. PubMed
The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble proteins for degradation. Xu Y, Anderson DE, Ye Y. Cell Discov. 2016 Nov 8;2:16040. eCollection 2016. PubMed

Associated Plasmids

Loss of maternal ANNEXIN A10 via a 34-kb deleted-type copy number variation is associated with embryonic mortality in Japanese Black cattle. Sasaki S, Ibi T, Akiyama T, Fukushima M, Sugimoto Y. BMC Genomics. 2016 Nov 24;17(1):968. PubMed
Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ. Aida T, Nakade S, Sakuma T, Izu Y, Oishi A, Mochida K, Ishikubo H, Usami T, Aizawa H, Yamamoto T, Tanaka K. BMC Genomics. 2016 Nov 28;17(1):979. PubMed
Efficient Generation of Orthologous Point Mutations in Pigs via CRISPR-assisted ssODN-mediated Homology-directed Repair. Wang K, Tang X, Liu Y, Xie Z, Zou X, Li M, Yuan H, Ouyang H, Jiao H, Pang D. Mol Ther Nucleic Acids. 2016 Nov 29;5(11):e396. doi: 10.1038/mtna.2016.101. PubMed
Deficiency of a Retinal Dystrophy Protein, Acyl-CoA Binding Domain-containing 5 (ACBD5), Impairs Peroxisomal beta-Oxidation of Very-long-chain Fatty Acids. Yagita Y, Shinohara K, Abe Y, Nakagawa K, Al-Owain M, Alkuraya FS, Fujiki Y. J Biol Chem. 2017 Jan 13;292(2):691-705. doi: 10.1074/jbc.M116.760090. Epub 2016 Nov 29. PubMed
An Ultraconserved Element (UCE) controls homeostatic splicing of ARGLU1 mRNA. Pirnie SP, Osman A, Zhu Y, Carmichael GG. Nucleic Acids Res. 2016 Nov 29. pii: gkw1140. PubMed
Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy. Yamashita SI, Jin X, Furukawa K, Hamasaki M, Nezu A, Otera H, Saigusa T, Yoshimori T, Sakai Y, Mihara K, Kanki T. J Cell Biol. 2016 Dec 5;215(5):649-665. Epub 2016 Nov 30. PubMed
A novel approach to assess the ubiquitin-fold modifier 1-system in cells. Ishimura R, Obata M, Kageyama S, Daniel J, Tanaka K, Komatsu M. FEBS Lett. 2017 Jan;591(1):196-204. doi: 10.1002/1873-3468.12518. Epub 2016 Dec 20. PubMed
Genome-Wide CRISPR Screen Identifies Regulators of Mitogen-Activated Protein Kinase as Suppressors of Liver Tumors in Mice. Song CQ, Li Y, Mou H, Moore J, Park A, Pomyen Y, Hough S, Kennedy Z, Fischer A, Yin H, Anderson DG, Conte D Jr, Zender L, Wang XW, Thorgeirsson S, Weng Z, Xue W. Gastroenterology. 2017 Apr;152(5):1161-1173.e1. doi: 10.1053/j.gastro.2016.12.002. Epub 2016 Dec 10. PubMed

Associated Plasmids

Loss of glyoxalase 1 induces compensatory mechanism to achieve dicarbonyl detoxification in mammalian Schwann cells. Morgenstern J, Fleming T, Schumacher D, Eckstein V, Freichel M, Herzig S, Nawroth P. J Biol Chem. 2016 Dec 12. pii: jbc.M116.760132. PubMed
Histone acetylation of glucose-induced thioredoxin-interacting protein gene expression in pancreatic islets. Bompada P, Atac D, Luan C, Andersson R, Omella JD, Laakso EO, Wright J, Groop L, De Marinis Y. Int J Biochem Cell Biol. 2016 Oct 29. pii: S1357-2725(16)30329-6. doi: 10.1016/j.biocel.2016.10.022. PubMed
A Critical Role for the Type I Interferon Receptor in Virus-Induced Autoimmune Diabetes in Rats. Qaisar N, Lin S, Ryan G, Yang C, Oikemus SR, Brodsky MH, Bortell R, Mordes JP, Wang JP. Diabetes. 2017 Jan;66(1):145-157. doi: 10.2337/db16-0462. Epub 2016 Oct 7. PubMed
CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins. Van Nostrand EL, Gelboin-Burkhart C, Wang R, Pratt GA, Blue SM, Yeo GW. Methods. 2016 Dec 18. pii: S1046-2023(16)30481-9. doi: 10.1016/j.ymeth.2016.12.007. PubMed
Deletion of the GAA repeats from the human frataxin gene using the CRISPR-Cas9 system in YG8R-derived cells and mouse models of Friedreich ataxia. Ouellet DL, Cherif K, Rousseau J, Tremblay JP. Gene Ther. 2017 May;24(5):265-274. doi: 10.1038/gt.2016.89. Epub 2016 Dec 26. PubMed
Using the CRISPR/Cas9 system to understand neuropeptide biology and regulation. Hay EA, Knowles C, Kolb A, MacKenzie A. Neuropeptides. 2016 Dec 3. pii: S0143-4179(16)30169-X. doi: 10.1016/j.npep.2016.11.010. PubMed
Inhibition of CRISPR-Cas9 with Bacteriophage Proteins. Rauch BJ, Silvis MR, Hultquist JF, Waters CS, McGregor MJ, Krogan NJ, Bondy-Denomy J. Cell. 2016 Dec 22. pii: S0092-8674(16)31683-X. doi: 10.1016/j.cell.2016.12.009. PubMed

Associated Plasmids

Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone. Gonen N, Quinn A, O'Neill HC, Koopman P, Lovell-Badge R. PLoS Genet. 2017 Jan 3;13(1):e1006520. doi: 10.1371/journal.pgen.1006520. eCollection 2017 Jan. PubMed
Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity. Zhao W, Tong H, Huang Y, Yan Y, Teng H, Xia Y, Jiang Q, Qin J. J Biol Chem. 2017 Feb 17;292(7):2773-2784. doi: 10.1074/jbc.M116.763961. Epub 2017 Jan 3. PubMed
Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides. Rivera-Torres N, Banas K, Bialk P, Bloh KM, Kmiec EB. PLoS One. 2017 Jan 4;12(1):e0169350. doi: 10.1371/journal.pone.0169350. eCollection 2017. PubMed
Contribution of canonical nonhomologous end joining to chromosomal rearrangements is enhanced by ATM kinase deficiency. Bhargava R, Carson CR, Lee G, Stark JM. Proc Natl Acad Sci U S A. 2017 Jan 5. pii: 201612204. doi: 10.1073/pnas.1612204114. PubMed
Functional Study of the Vitamin K Cycle Enzymes in Live Cells. Tie JK, Stafford DW. Methods Enzymol. 2017;584:349-394. doi: 10.1016/bs.mie.2016.10.015. Epub 2016 Nov 22. PubMed
Temporally-Distinct PD-L1 Expression by Tumor and Host Cells Contributes to Immune Escape. Noguchi T, Ward JP, Gubin MM, Arthur CD, Lee SH, Hundal J, Selby M, Graziano RF, Mardis ER, Korman AJ, Schreiber RD. Cancer Immunol Res. 2017 Jan 10. pii: canimm.0391.2016. doi: 10.1158/2326-6066.CIR-16-0391. PubMed
Generation of a Stable Transgenic Swine Model Expressing a Porcine Histone 2B-eGFP Fusion Protein for Cell Tracking and Chromosome Dynamics Studies. Sper RB, Koh S, Zhang X, Simpson S, Collins B, Sommer J, Petters RM, Caballero I, Platt JL, Piedrahita JA. PLoS One. 2017 Jan 12;12(1):e0169242. doi: 10.1371/journal.pone.0169242. eCollection 2017. PubMed
Maternal Supply of Cas9 to Zygotes Facilitates the Efficient Generation of Site-Specific Mutant Mouse Models. Cebrian-Serrano A, Zha S, Hanssen L, Biggs D, Preece C, Davies B. PLoS One. 2017 Jan 12;12(1):e0169887. doi: 10.1371/journal.pone.0169887. eCollection 2017. PubMed
Noncanonical function of DGCR8 controls mESC exit from pluripotency. Cirera-Salinas D, Yu J, Bodak M, Ngondo RP, Herbert KM, Ciaudo C. J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073. PubMed

Associated Plasmids

Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice. Fu X, Zhang L, Jin Y, Sun X, Zhang A, Wen Z, Zhou Y, Xia M, Gao J. Neural Plast. 2016;2016:6720420. doi: 10.1155/2016/6720420. Epub 2016 Dec 22. PubMed
SOX10-Nano-Lantern Reporter Human iPS Cells; A Versatile Tool for Neural Crest Research. Horikiri T, Ohi H, Shibata M, Ikeya M, Ueno M, Sotozono C, Kinoshita S, Sato T. PLoS One. 2017 Jan 20;12(1):e0170342. doi: 10.1371/journal.pone.0170342. eCollection 2017. PubMed
The application of CRISPR technology to high content screening in primary neurons. Callif BL, Maunze B, Krueger NL, Simpson MT, Blackmore MG. Mol Cell Neurosci. 2017 Apr;80:170-179. doi: 10.1016/j.mcn.2017.01.003. Epub 2017 Jan 19. PubMed
An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Golden RJ, Chen B, Li T, Braun J, Manjunath H, Chen X, Wu J, Schmid V, Chang TC, Kopp F, Ramirez-Martinez A, Tagliabracci VS, Chen ZJ, Xie Y, Mendell JT. Nature. 2017 Feb 9;542(7640):197-202. doi: 10.1038/nature21025. Epub 2017 Jan 23. PubMed

Associated Plasmids

Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing. Goldfarb KC, Cech TR. Genes Dev. 2017 Jan 1;31(1):59-71. doi: 10.1101/gad.286963.116. Epub 2017 Jan 23. PubMed

Associated Plasmids

Human BCDIN3D monomethylates cytoplasmic histidine transfer RNA. Martinez A, Yamashita S, Nagaike T, Sakaguchi Y, Suzuki T, Tomita K. Nucleic Acids Res. 2017 May 19;45(9):5423-5436. doi: 10.1093/nar/gkx051. PubMed
PLCE1 Promotes Esophageal Cancer Cell Progression by Maintaining the Transcriptional Activity of Snail. Zhai S, Liu C, Zhang L, Zhu J, Guo J, Zhang J, Chen Z, Zhou W, Chang T, Xu S, Qi Y, Zhuang T, Yu N, Wang W, Wang H, Yu S, Li X. Neoplasia. 2017 Mar;19(3):154-164. doi: 10.1016/j.neo.2016.12.007. Epub 2017 Jan 29. PubMed
Genome Editing Reveals Glioblastoma Addiction to MicroRNA-10b. El Fatimy R, Subramanian S, Uhlmann EJ, Krichevsky AM. Mol Ther. 2017 Feb 1;25(2):368-378. doi: 10.1016/j.ymthe.2016.11.004. PubMed
Polycomb Repressive Complex 1 Generates Discrete Compacted Domains that Change during Differentiation. Kundu S, Ji F, Sunwoo H, Jain G, Lee JT, Sadreyev RI, Dekker J, Kingston RE. Mol Cell. 2017 Feb 2;65(3):432-446.e5. doi: 10.1016/j.molcel.2017.01.009. PubMed
An interaction domain in human SAMD9 is essential for myxoma virus host-range determinant M062 antagonism of host anti-viral function. Nounamo B, Li Y, O'Byrne P, Kearney AM, Khan A, Liu J. Virology. 2017 Mar;503:94-102. doi: 10.1016/j.virol.2017.01.004. Epub 2017 Jan 31. PubMed
An Efficient Electroporation Protocol for the Genetic Modification of Mammalian Cells. Chicaybam L, Barcelos C, Peixoto B, Carneiro M, Limia CG, Redondo P, Lira C, Paraguassu-Braga F, Vasconcelos ZF, Barros L, Bonamino MH. Front Bioeng Biotechnol. 2017 Jan 23;4:99. doi: 10.3389/fbioe.2016.00099. eCollection 2016. PubMed

Associated Plasmids

Impaired striatal dopamine release in homozygous Vps35 D620N knock-in mice. Ishizu N, Yui D, Hebisawa A, Aizawa H, Cui W, Fujita Y, Hashimoto K, Ajioka I, Mizusawa H, Yokota T, Watase K. Hum Mol Genet. 2016 Oct 15;25(20):4507-4517. doi: 10.1093/hmg/ddw279. PubMed
Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes. Staahl BT, Benekareddy M, Coulon-Bainier C, Banfal AA, Floor SN, Sabo JK, Urnes C, Munares GA, Ghosh A, Doudna JA. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806. PubMed

Associated Plasmids

MicroRNA 301A Promotes Intestinal Inflammation and Colitis-Associated Cancer Development by Inhibiting BTG1. He C, Yu T, Shi Y, Ma C, Yang W, Fang L, Sun M, Wu W, Xiao F, Guo F, Chen M, Yang H, Qian J, Cong Y, Liu Z. Gastroenterology. 2017 May;152(6):1434-1448.e15. doi: 10.1053/j.gastro.2017.01.049. Epub 2017 Feb 11. PubMed
CBX3 promotes colon cancer cell proliferation by CDK6 kinase-independent function during cell cycle. Fan Y, Li H, Liang X, Xiang Z. Oncotarget. 2017 Mar 21;8(12):19934-19946. doi: 10.18632/oncotarget.15253. PubMed
CRISPR/Cas9-mediated genome editing in wild-derived mice: generation of tamed wild-derived strains by mutation of the a (nonagouti) gene. Hirose M, Hasegawa A, Mochida K, Matoba S, Hatanaka Y, Inoue K, Goto T, Kaneda H, Yamada I, Furuse T, Abe K, Uenoyama Y, Tsukamura H, Wakana S, Honda A, Ogura A. Sci Rep. 2017 Feb 14;7:42476. doi: 10.1038/srep42476. PubMed
Biallelic insertion of a transcriptional terminator via the CRISPR/Cas9 system efficiently silences expression of protein-coding and non-coding RNA genes. Liu Y, Han X, Yuan J, Geng T, Chen S, Hu X, Cui IH, Cui H. J Biol Chem. 2017 Apr 7;292(14):5624-5633. doi: 10.1074/jbc.M116.769034. Epub 2017 Feb 14. PubMed
Distinct C-mannosylation of netrin receptor thrombospondin type 1 repeats by mammalian DPY19L1 and DPY19L3. Shcherbakova A, Tiemann B, Buettner FF, Bakker H. Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):2574-2579. doi: 10.1073/pnas.1613165114. Epub 2017 Feb 15. PubMed
Luman contributes to brefeldin A-induced prion protein gene expression by interacting with the ERSE26 element. Dery MA, LeBlanc AC. Sci Rep. 2017 Feb 13;7:42285. doi: 10.1038/srep42285. PubMed
A novel LSD1 inhibitor NCD38 ameliorates MDS-related leukemia with complex karyotype by attenuating leukemia programs via activating super-enhancers. Sugino N, Kawahara M, Tatsumi G, Kanai A, Matsui H, Yamamoto R, Nagai Y, Fujii S, Shimazu Y, Hishizawa M, Inaba T, Andoh A, Suzuki T, Takaori-Kondo A. Leukemia. 2017 Nov;31(11):2303-2314. doi: 10.1038/leu.2017.59. Epub 2017 Feb 17. PubMed
Conformational Profiling of the AT1 Angiotensin II Receptor Reflects Biased Agonism, G Protein Coupling, and Cellular Context. Devost D, Sleno R, Petrin D, Zhang A, Shinjo Y, Okde R, Aoki J, Inoue A, Hebert TE. J Biol Chem. 2017 Mar 31;292(13):5443-5456. doi: 10.1074/jbc.M116.763854. Epub 2017 Feb 17. PubMed
Somatic Mutations in TSC1 and TSC2 Cause Focal Cortical Dysplasia. Lim JS, Gopalappa R, Kim SH, Ramakrishna S, Lee M, Kim WI, Kim J, Park SM, Lee J, Oh JH, Kim HD, Park CH, Lee JS, Kim S, Kim DS, Han JM, Kang HC, Kim HH, Lee JH. Am J Hum Genet. 2017 Mar 2;100(3):454-472. doi: 10.1016/j.ajhg.2017.01.030. Epub 2017 Feb 16. PubMed
Targeted RP9 ablation and mutagenesis in mouse photoreceptor cells by CRISPR-Cas9. Lv JN, Zhou GH, Chen X, Chen H, Wu KC, Xiang L, Lei XL, Zhang X, Wu RH, Jin ZB. Sci Rep. 2017 Feb 20;7:43062. doi: 10.1038/srep43062. PubMed
Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection. Mitzelfelt KA, McDermott-Roe C, Grzybowski MN, Marquez M, Kuo CT, Riedel M, Lai S, Choi MJ, Kolander KD, Helbling D, Dimmock DP, Battle MA, Jou CJ, Tristani-Firouzi M, Verbsky JW, Benjamin IJ, Geurts AM. Stem Cell Reports. 2017 Mar 14;8(3):491-499. doi: 10.1016/j.stemcr.2017.01.021. Epub 2017 Feb 24. PubMed
Systems-based analysis of RIG-I-dependent signalling identifies KHSRP as an inhibitor of RIG-I receptor activation. Soonthornvacharin S, Rodriguez-Frandsen A, Zhou Y, Galvez F, Huffmaster NJ, Tripathi S, Balasubramaniam VR, Inoue A, de Castro E, Moulton H, Stein DA, Sanchez-Aparicio MT, De Jesus PD, Nguyen Q, Konig R, Krogan NJ, Garcia-Sastre A, Yoh SM, Chanda SK. Nat Microbiol. 2017 Mar 1;2:17022. doi: 10.1038/nmicrobiol.2017.22. PubMed
Inhibition of CRISPR/Cas9-Mediated Genome Engineering by a Type I Interferon-Induced Reduction in Guide RNA Expression. Machitani M, Sakurai F, Wakabayashi K, Nakatani K, Takayama K, Tachibana M, Mizuguchi H. Biol Pharm Bull. 2017;40(3):272-277. doi: 10.1248/bpb.b16-00700. PubMed
Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs. Ferry QR, Lyutova R, Fulga TA. Nat Commun. 2017 Mar 3;8:14633. doi: 10.1038/ncomms14633. PubMed
Substrate specificity of TOR complex 2 is determined by a ubiquitin-fold domain of the Sin1 subunit. Tatebe H, Murayama S, Yonekura T, Hatano T, Richter D, Furuya T, Kataoka S, Furuita K, Kojima C, Shiozaki K. Elife. 2017 Mar 7;6. pii: e19594. doi: 10.7554/eLife.19594. PubMed
The Product of the Herpes Simplex Virus 2 UL16 Gene Is Critical for the Egress of Capsids from the Nuclei of Infected Cells. Gao J, Hay TJM, Banfield BW. J Virol. 2017 Apr 28;91(10). pii: e00350-17. doi: 10.1128/JVI.00350-17. Print 2017 May 15. PubMed
CRISPR-Cas9 system-driven site-specific selection pressure on Herpes simplex virus genomes. Li Z, Bi Y, Xiao H, Sun L, Ren Y, Li Y, Chen C, Cun W. Virus Res. 2017 Mar 6. pii: S0168-1702(16)30694-3. doi: 10.1016/j.virusres.2017.03.010. PubMed
Identification of common non-coding variants at 1p22 that are functional for non-syndromic orofacial clefting. Liu H, Leslie EJ, Carlson JC, Beaty TH, Marazita ML, Lidral AC, Cornell RA. Nat Commun. 2017 Mar 13;8:14759. doi: 10.1038/ncomms14759. PubMed
Loss of LMOD1 impairs smooth muscle cytocontractility and causes megacystis microcolon intestinal hypoperistalsis syndrome in humans and mice. Halim D, Wilson MP, Oliver D, Brosens E, Verheij JB, Han Y, Nanda V, Lyu Q, Doukas M, Stoop H, Brouwer RW, van IJcken WF, Slivano OJ, Burns AJ, Christie CK, de Mesy Bentley KL, Brooks AS, Tibboel D, Xu S, Jin ZG, Djuwantono T, Yan W, Alves MM, Hofstra RM, Miano JM. Proc Natl Acad Sci U S A. 2017 Mar 28;114(13):E2739-E2747. doi: 10.1073/pnas.1620507114. Epub 2017 Mar 14. PubMed
Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. Balczon R, Morrow KA, Zhou C, Edmonds B, Alexeyev M, Pittet JF, Wagener BM, Moser SA, Leavesley S, Zha X, Frank DW, Stevens T. FASEB J. 2017 Jul;31(7):2785-2796. doi: 10.1096/fj.201601042RR. Epub 2017 Mar 17. PubMed
DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression. Barr AR, Cooper S, Heldt FS, Butera F, Stoy H, Mansfeld J, Novak B, Bakal C. Nat Commun. 2017 Mar 20;8:14728. doi: 10.1038/ncomms14728. PubMed
Chd7 is indispensable for mammalian brain development through activation of a neuronal differentiation programme. Feng W, Kawauchi D, Korkel-Qu H, Deng H, Serger E, Sieber L, Lieberman JA, Jimeno-Gonzalez S, Lambo S, Hanna BS, Harim Y, Jansen M, Neuerburg A, Friesen O, Zuckermann M, Rajendran V, Gronych J, Ayrault O, Korshunov A, Jones DT, Kool M, Northcott PA, Lichter P, Cortes-Ledesma F, Pfister SM, Liu HK. Nat Commun. 2017 Mar 20;8:14758. doi: 10.1038/ncomms14758. PubMed
TRH Action Is Impaired in Pituitaries of Male IGSF1-Deficient Mice. Turgeon MO, Silander TL, Doycheva D, Liao XH, Rigden M, Ongaro L, Zhou X, Joustra SD, Wit JM, Wade MG, Heuer H, Refetoff S, Bernard DJ. Endocrinology. 2017 Apr 1;158(4):815-830. doi: 10.1210/en.2016-1788. PubMed
Increased Expression of Laminin Subunit Alpha 1 Chain by dCas9-VP160. Perrin A, Rousseau J, Tremblay JP. Mol Ther Nucleic Acids. 2017 Mar 17;6:68-79. doi: 10.1016/j.omtn.2016.11.004. Epub 2016 Dec 10. PubMed
Sensing of Bacterial Cyclic Dinucleotides by the Oxidoreductase RECON Promotes NF-kappaB Activation and Shapes a Proinflammatory Antibacterial State. McFarland AP, Luo S, Ahmed-Qadri F, Zuck M, Thayer EF, Goo YA, Hybiske K, Tong L, Woodward JJ. Immunity. 2017 Mar 21;46(3):433-445. doi: 10.1016/j.immuni.2017.02.014. PubMed
Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery. Gaj T, Staahl BT, Rodrigues GM, Limsirichai P, Ekman FK, Doudna JA, Schaffer DV. Nucleic Acids Res. 2017 Mar 2. doi: 10.1093/nar/gkx154. PubMed
Circ-ZNF609 Is a Circular RNA that Can Be Translated and Functions in Myogenesis. Legnini I, Di Timoteo G, Rossi F, Morlando M, Briganti F, Sthandier O, Fatica A, Santini T, Andronache A, Wade M, Laneve P, Rajewsky N, Bozzoni I. Mol Cell. 2017 Apr 6;66(1):22-37.e9. doi: 10.1016/j.molcel.2017.02.017. Epub 2017 Mar 23. PubMed
Visualization and targeting of LGR5(+) human colon cancer stem cells. Shimokawa M, Ohta Y, Nishikori S, Matano M, Takano A, Fujii M, Date S, Sugimoto S, Kanai T, Sato T. Nature. 2017 May 11;545(7653):187-192. doi: 10.1038/nature22081. Epub 2017 Mar 29. PubMed
BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, Kumanogoh A, Murakami M, Ikawa M, Takeda K. J Exp Med. 2017 May 1;214(5):1313-1331. doi: 10.1084/jem.20161076. Epub 2017 Mar 29. PubMed
Sialylation Is Dispensable for Early Murine Embryonic Development in Vitro. Abeln M, Borst KM, Cajic S, Thiesler H, Kats E, Albers I, Kuhn M, Kaever V, Rapp E, Munster-Kuhnel A, Weinhold B. Chembiochem. 2017 Jul 4;18(13):1305-1316. doi: 10.1002/cbic.201700083. Epub 2017 May 11. PubMed
Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency. Yang Y, Liu B, Xu J, Wang J, Wu J, Shi C, Xu Y, Dong J, Wang C, Lai W, Zhu J, Xiong L, Zhu D, Li X, Yang W, Yamauchi T, Sugawara A, Li Z, Sun F, Li X, Li C, He A, Du Y, Wang T, Zhao C, Li H, Chi X, Zhang H, Liu Y, Li C, Duo S, Yin M, Shen H, Belmonte JCI, Deng H. Cell. 2017 Apr 6;169(2):243-257.e25. doi: 10.1016/j.cell.2017.02.005. PubMed
The BET Protein BRD2 Cooperates with CTCF to Enforce Transcriptional and Architectural Boundaries. Hsu SC, Gilgenast TG, Bartman CR, Edwards CR, Stonestrom AJ, Huang P, Emerson DJ, Evans P, Werner MT, Keller CA, Giardine B, Hardison RC, Raj A, Phillips-Cremins JE, Blobel GA. Mol Cell. 2017 Apr 6;66(1):102-116.e7. doi: 10.1016/j.molcel.2017.02.027. PubMed
Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells. Yan Y, Zhao W, Huang Y, Tong H, Xia Y, Jiang Q, Qin J. Sci Rep. 2017 Apr 10;7:46276. doi: 10.1038/srep46276. PubMed
Association of C-Type Lectin Mincle with FcepsilonRIbetagamma Subunits Leads to Functional Activation of RBL-2H3 Cells through Syk. Honjoh C, Chihara K, Yoshiki H, Yamauchi S, Takeuchi K, Kato Y, Hida Y, Ishizuka T, Sada K. Sci Rep. 2017 Apr 10;7:46064. doi: 10.1038/srep46064. PubMed
CRISPR/Cas9-Mediated Gene Knockout in the Mouse Brain Using In Utero Electroporation. Shinmyo Y, Kawasaki H. Curr Protoc Neurosci. 2017 Apr 10;79:3.32.1-3.32.11. doi: 10.1002/cpns.26. PubMed
Unusual semi-extractability as a hallmark of nuclear body-associated architectural noncoding RNAs. Chujo T, Yamazaki T, Kawaguchi T, Kurosaka S, Takumi T, Nakagawa S, Hirose T. EMBO J. 2017 May 15;36(10):1447-1462. doi: 10.15252/embj.201695848. Epub 2017 Apr 12. PubMed
Genome-Wide Screening Uncovers the Significance of N-Sulfation of Heparan Sulfate as a Host Cell Factor for Chikungunya Virus Infection. Tanaka A, Tumkosit U, Nakamura S, Motooka D, Kishishita N, Priengprom T, Sa-Ngasang A, Kinoshita T, Takeda N, Maeda Y. J Virol. 2017 Jun 9;91(13). pii: e00432-17. doi: 10.1128/JVI.00432-17. Print 2017 Jul 1. PubMed
Foot-and-mouth disease virus infection inhibits LGP2 protein expression to exaggerate inflammatory response and promote viral replication. Zhu Z, Li C, Du X, Wang G, Cao W, Yang F, Feng H, Zhang X, Shi Z, Liu H, Tian H, Li D, Zhang K, Liu X, Zheng H. Cell Death Dis. 2017 Apr 13;8(4):e2747. doi: 10.1038/cddis.2017.170. PubMed
Identification of a novel functional JAK1 S646P mutation in acute lymphoblastic leukemia. Li Q, Li B, Hu L, Ning H, Jiang M, Wang D, Liu T, Zhang B, Chen H. Oncotarget. 2017 May 23;8(21):34687-34697. doi: 10.18632/oncotarget.16670. PubMed
A tiling-deletion-based genetic screen for cis-regulatory element identification in mammalian cells. Diao Y, Fang R, Li B, Meng Z, Yu J, Qiu Y, Lin KC, Huang H, Liu T, Marina RJ, Jung I, Shen Y, Guan KL, Ren B. Nat Methods. 2017 Jun;14(6):629-635. doi: 10.1038/nmeth.4264. Epub 2017 Apr 17. PubMed
An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system. Niccheri F, Pecori R, Conticello SG. Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y. PubMed

Associated Plasmids

Minus end-directed kinesin-14 KIFC1 regulates the positioning and architecture of the Golgi apparatus. She ZY, Pan MY, Tan FQ, Yang WX. Oncotarget. 2017 May 30;8(22):36469-36483. doi: 10.18632/oncotarget.16863. PubMed
A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis. Treiber T, Treiber N, Plessmann U, Harlander S, Daiss JL, Eichner N, Lehmann G, Schall K, Urlaub H, Meister G. Mol Cell. 2017 Apr 20;66(2):270-284.e13. doi: 10.1016/j.molcel.2017.03.014. PubMed
Identification of subepithelial mesenchymal cells that induce IgA and diversify gut microbiota. Nagashima K, Sawa S, Nitta T, Tsutsumi M, Okamura T, Penninger JM, Nakashima T, Takayanagi H. Nat Immunol. 2017 Jun;18(6):675-682. doi: 10.1038/ni.3732. Epub 2017 Apr 24. PubMed
SWELL1 is a regulator of adipocyte size, insulin signalling and glucose homeostasis. Zhang Y, Xie L, Gunasekar SK, Tong D, Mishra A, Gibson WJ, Wang C, Fidler T, Marthaler B, Klingelhutz A, Abel ED, Samuel I, Smith JK, Cao L, Sah R. Nat Cell Biol. 2017 May;19(5):504-517. doi: 10.1038/ncb3514. Epub 2017 Apr 24. PubMed
PARP3 is a promoter of chromosomal rearrangements and limits G4 DNA. Day TA, Layer JV, Cleary JP, Guha S, Stevenson KE, Tivey T, Kim S, Schinzel AC, Izzo F, Doench J, Root DE, Hahn WC, Price BD, Weinstock DM. Nat Commun. 2017 Apr 27;8:15110. doi: 10.1038/ncomms15110. PubMed
Construction of a highly efficient CRISPR/Cas9-mediated duck enteritis virus-based vaccine against H5N1 avian influenza virus and duck Tembusu virus infection. Zou Z, Huang K, Wei Y, Chen H, Liu Z, Jin M. Sci Rep. 2017 May 3;7(1):1478. doi: 10.1038/s41598-017-01554-1. PubMed
ALKBH1 is an RNA dioxygenase responsible for cytoplasmic and mitochondrial tRNA modifications. Kawarada L, Suzuki T, Ohira T, Hirata S, Miyauchi K, Suzuki T. Nucleic Acids Res. 2017 May 2. doi: 10.1093/nar/gkx354. PubMed
The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension. Haarhuis JHI, van der Weide RH, Blomen VA, Yanez-Cuna JO, Amendola M, van Ruiten MS, Krijger PHL, Teunissen H, Medema RH, van Steensel B, Brummelkamp TR, de Wit E, Rowland BD. Cell. 2017 May 4;169(4):693-707.e14. doi: 10.1016/j.cell.2017.04.013. PubMed
A lentivirus-free inducible CRISPR-Cas9 system for efficient targeting of human genes. Bisht K, Grill S, Graniel J, Nandakumar J. Anal Biochem. 2017 Aug 1;530:40-49. doi: 10.1016/j.ab.2017.05.001. Epub 2017 May 4. PubMed
Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Natsume T, Nishimura K, Minocherhomji S, Bhowmick R, Hickson ID, Kanemaki MT. Genes Dev. 2017 Apr 15;31(8):816-829. doi: 10.1101/gad.297663.117. Epub 2017 May 9. PubMed
Proline metabolism supports metastasis formation and could be inhibited to selectively target metastasizing cancer cells. Elia I, Broekaert D, Christen S, Boon R, Radaelli E, Orth MF, Verfaillie C, Grunewald TGP, Fendt SM. Nat Commun. 2017 May 11;8:15267. doi: 10.1038/ncomms15267. PubMed
Characterization of miR-122-independent propagation of HCV. Ono C, Fukuhara T, Motooka D, Nakamura S, Okuzaki D, Yamamoto S, Tamura T, Mori H, Sato A, Uemura K, Fauzyah Y, Kurihara T, Suda T, Nishio A, Hmwe SS, Okamoto T, Tatsumi T, Takehara T, Chayama K, Wakita T, Koike K, Matsuura Y. PLoS Pathog. 2017 May 11;13(5):e1006374. doi: 10.1371/journal.ppat.1006374. eCollection 2017 May. PubMed
The murine catecholamine methyltransferase mTOMT is essential for mechanotransduction by cochlear hair cells. Cunningham CL, Wu Z, Jafari A, Zhao B, Schrode K, Harkins-Perry S, Lauer A, Muller U. Elife. 2017 May 15;6. pii: e24318. doi: 10.7554/eLife.24318. PubMed
TRAF3 enhances TCR signaling by regulating the inhibitors Csk and PTPN22. Wallis AM, Wallace EC, Hostager BS, Yi Z, Houtman JCD, Bishop GA. Sci Rep. 2017 May 18;7(1):2081. doi: 10.1038/s41598-017-02280-4. PubMed
Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Yao X, Wang X, Hu X, Liu Z, Liu J, Zhou H, Shen X, Wei Y, Huang Z, Ying W, Wang Y, Nie YH, Zhang CC, Li S, Cheng L, Wang Q, Wu Y, Huang P, Sun Q, Shi L, Yang H. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76. PubMed

Associated Plasmids

Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur N, Dekker J, Mirny LA, Bruneau BG. Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004. PubMed

Associated Plasmids

CRISPR/Cas9 - Mediated Precise Targeted Integration In Vivo Using a Double Cut Donor with Short Homology Arms. Yao X, Wang X, Liu J, Hu X, Shi L, Shen X, Ying W, Sun X, Wang X, Huang P, Yang H. EBioMedicine. 2017 Jun;20:19-26. doi: 10.1016/j.ebiom.2017.05.015. Epub 2017 May 11. PubMed
Hit and go CAS9 delivered through a lentiviral based self-limiting circuit. Petris G, Casini A, Montagna C, Lorenzin F, Prandi D, Romanel A, Zasso J, Conti L, Demichelis F, Cereseto A. Nat Commun. 2017 May 22;8:15334. doi: 10.1038/ncomms15334. PubMed
CD7-edited T cells expressing a CD7-specific CAR for the therapy of T-cell malignancies. Gomes-Silva D, Srinivasan M, Sharma S, Lee CM, Wagner DL, Davis TH, Rouce RH, Bao G, Brenner MK, Mamonkin M. Blood. 2017 Jul 20;130(3):285-296. doi: 10.1182/blood-2017-01-761320. Epub 2017 May 24. PubMed
Extracellular Interactions between Hepatitis C Virus and Secreted Apolipoprotein E. Li Z, Li Y, Bi Y, Zhang H, Yao Y, Li Q, Cun W, Dong S. J Virol. 2017 Jul 12;91(15). pii: e02227-16. doi: 10.1128/JVI.02227-16. Print 2017 Aug 1. PubMed
Efficient generation of targeted and controlled mutational events in porcine cells using nuclease-directed homologous recombination. Butler JR, Santos RMN, Martens GR, Ladowski JM, Wang ZY, Li P, Tector M, Tector AJ. J Surg Res. 2017 May 15;212:238-245. doi: 10.1016/j.jss.2017.01.025. Epub 2017 Jan 29. PubMed
Necroptosis and ferroptosis are alternative cell death pathways that operate in acute kidney failure. Muller T, Dewitz C, Schmitz J, Schroder AS, Brasen JH, Stockwell BR, Murphy JM, Kunzendorf U, Krautwald S. Cell Mol Life Sci. 2017 May 27. doi: 10.1007/s00018-017-2547-4. PubMed
Generation of carbamoyl phosphate synthetase 1 reporter cell lines for the assessment of ammonia metabolism. Wang Y, Chang L, Zhai J, Wu Q, Wang D, Wang Y. J Cell Mol Med. 2017 May 30. doi: 10.1111/jcmm.13225. PubMed
Argininosuccinate synthase 1 is an intrinsic Akt repressor transactivated by p53. Miyamoto T, Lo PHY, Saichi N, Ueda K, Hirata M, Tanikawa C, Matsuda K. Sci Adv. 2017 May 19;3(5):e1603204. doi: 10.1126/sciadv.1603204. eCollection 2017 May. PubMed
Systematic discovery of mutation-specific synthetic lethals by mining pan-cancer human primary tumor data. Sinha S, Thomas D, Chan S, Gao Y, Brunen D, Torabi D, Reinisch A, Hernandez D, Chan A, Rankin EB, Bernards R, Majeti R, Dill DL. Nat Commun. 2017 May 31;8:15580. doi: 10.1038/ncomms15580. PubMed
Genetic wiring maps of single-cell protein states reveal an off-switch for GPCR signalling. Brockmann M, Blomen VA, Nieuwenhuis J, Stickel E, Raaben M, Bleijerveld OB, Altelaar AFM, Jae LT, Brummelkamp TR. Nature. 2017 Jun 8;546(7657):307-311. doi: 10.1038/nature22376. Epub 2017 May 31. PubMed
Proof-of-Concept Gene Editing for the Murine Model of Inducible Arginase-1 Deficiency. Sin YY, Price PR, Ballantyne LL, Funk CD. Sci Rep. 2017 May 31;7(1):2585. doi: 10.1038/s41598-017-02927-2. PubMed
A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids. Beneke T, Madden R, Makin L, Valli J, Sunter J, Gluenz E. R Soc Open Sci. 2017 May 3;4(5):170095. doi: 10.1098/rsos.170095. eCollection 2017 May. PubMed
MyoD- and FoxO3-mediated hotspot interaction orchestrates super-enhancer activity during myogenic differentiation. Peng XL, So KK, He L, Zhao Y, Zhou J, Li Y, Yao M, Xu B, Zhang S, Yao H, Hu P, Sun H, Wang H. Nucleic Acids Res. 2017 Jun 1. doi: 10.1093/nar/gkx488. PubMed
The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Simsek D, Tiu GC, Flynn RA, Byeon GW, Leppek K, Xu AF, Chang HY, Barna M. Cell. 2017 Jun 1;169(6):1051-1065.e18. doi: 10.1016/j.cell.2017.05.022. PubMed
CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations. Kuscu C, Parlak M, Tufan T, Yang J, Szlachta K, Wei X, Mammadov R, Adli M. Nat Methods. 2017 Jul;14(7):710-712. doi: 10.1038/nmeth.4327. Epub 2017 Jun 5. PubMed
Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System. Zhang C, Lu L. Methods Mol Biol. 2017;1625:249-258. doi: 10.1007/978-1-4939-7104-6_17. PubMed
Detection of genome-edited mutant clones by a simple competition-based PCR method. Harayama T, Riezman H. PLoS One. 2017 Jun 6;12(6):e0179165. doi: 10.1371/journal.pone.0179165. eCollection 2017. PubMed
Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets. Lu X, Nowicka U, Sridharan V, Liu F, Randles L, Hymel D, Dyba M, Tarasov SG, Tarasova NI, Zhao XZ, Hamazaki J, Murata S, Burke TR Jr, Walters KJ. Nat Commun. 2017 Jun 9;8:15540. doi: 10.1038/ncomms15540. PubMed
Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition. Guo LY, Allu PK, Zandarashvili L, McKinley KL, Sekulic N, Dawicki-McKenna JM, Fachinetti D, Logsdon GA, Jamiolkowski RM, Cleveland DW, Cheeseman IM, Black BE. Nat Commun. 2017 Jun 9;8:15775. doi: 10.1038/ncomms15775. PubMed
Regulated Erlin-dependent release of the B12 transmembrane J-protein promotes ER membrane penetration of a non-enveloped virus. Inoue T, Tsai B. PLoS Pathog. 2017 Jun 14;13(6):e1006439. doi: 10.1371/journal.ppat.1006439. eCollection 2017 Jun. PubMed
CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Mou H, Smith JL, Peng L, Yin H, Moore J, Zhang XO, Song CQ, Sheel A, Wu Q, Ozata DM, Li Y, Anderson DG, Emerson CP, Sontheimer EJ, Moore MJ, Weng Z, Xue W. Genome Biol. 2017 Jun 14;18(1):108. doi: 10.1186/s13059-017-1237-8. PubMed
Sexually dimorphic distribution of Prokr2 neurons revealed by the Prokr2-Cre mouse model. Mohsen Z, Sim H, Garcia-Galiano D, Han X, Bellefontaine N, Saunders TL, Elias CF. Brain Struct Funct. 2017 Jun 14. doi: 10.1007/s00429-017-1456-5. PubMed
Critical role of CREBH-mediated induction of TGF-beta2 by HCV infection in fibrogenic responses in hepatic stellate cells. Chida T, Ito M, Nakashima K, Kanegae Y, Aoshima T, Takabayashi S, Kawata K, Nakagawa Y, Yamamoto M, Shimano H, Matsuura T, Kobayashi Y, Suda T, Suzuki T. Hepatology. 2017 Jun 16. doi: 10.1002/hep.29319. PubMed
CRISPR/Cas9-loxP-Mediated Gene Editing as a Novel Site-Specific Genetic Manipulation Tool. Yang F, Liu C, Chen D, Tu M, Xie H, Sun H, Ge X, Tang L, Li J, Zheng J, Song Z, Qu J, Gu F. Mol Ther Nucleic Acids. 2017 Jun 16;7:378-386. doi: 10.1016/j.omtn.2017.04.018. Epub 2017 Apr 25. PubMed
Genetic evidence that beta-arrestins are dispensable for the initiation of beta2-adrenergic receptor signaling to ERK. O'Hayre M, Eichel K, Avino S, Zhao X, Steffen DJ, Feng X, Kawakami K, Aoki J, Messer K, Sunahara R, Inoue A, von Zastrow M, Gutkind JS. Sci Signal. 2017 Jun 20;10(484). pii: 10/484/eaal3395. doi: 10.1126/scisignal.aal3395. PubMed
Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids. Noda T, Oji A, Ikawa M. Methods Mol Biol. 2017;1630:67-80. doi: 10.1007/978-1-4939-7128-2_6. PubMed
Host-derived apolipoproteins play comparable roles with viral secretory proteins Erns and NS1 in the infectious particle formation of Flaviviridae. Fukuhara T, Tamura T, Ono C, Shiokawa M, Mori H, Uemura K, Yamamoto S, Kurihara T, Okamoto T, Suzuki R, Yoshii K, Kurosu T, Igarashi M, Aoki H, Sakoda Y, Matsuura Y. PLoS Pathog. 2017 Jun 23;13(6):e1006475. doi: 10.1371/journal.ppat.1006475. eCollection 2017 Jun. PubMed
Generation of Genetically Modified Mice through the Microinjection of Oocytes. Delerue F, Ittner LM. J Vis Exp. 2017 Jun 15;(124). doi: 10.3791/55765. PubMed
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells. Sternburg EL, Dias KC, Karginov FV. J Vis Exp. 2017 Jun 16;(124). doi: 10.3791/55903. PubMed
TSPAN12 Is a Norrin Co-receptor that Amplifies Frizzled4 Ligand Selectivity and Signaling. Lai MB, Zhang C, Shi J, Johnson V, Khandan L, McVey J, Klymkowsky MW, Chen Z, Junge HJ. Cell Rep. 2017 Jun 27;19(13):2809-2822. doi: 10.1016/j.celrep.2017.06.004. PubMed

Associated Plasmids

CEP19 cooperates with FOP and CEP350 to drive early steps in the ciliogenesis programme. Mojarad BA, Gupta GD, Hasegan M, Goudiam O, Basto R, Gingras AC, Pelletier L. Open Biol. 2017 Jun;7(6). pii: rsob.170114. doi: 10.1098/rsob.170114. PubMed
A novel humanized mouse lacking murine P450 oxidoreductase for studying human drug metabolism. Barzi M, Pankowicz FP, Zorman B, Liu X, Legras X, Yang D, Borowiak M, Bissig-Choisat B, Sumazin P, Li F, Bissig KD. Nat Commun. 2017 Jun 28;8(1):39. doi: 10.1038/s41467-017-00049-x. PubMed
Multiplexed gene editing and protein over-expression using a Tobacco mosaic virus viral vector. Cody W, Scholthof HB, Mirkov TE. Plant Physiol. 2017 Jun 29. pii: pp.00411.2017. doi: 10.1104/pp.17.00411. PubMed
Inherited determinants of early recurrent somatic mutations in prostate cancer. Romanel A, Garritano S, Stringa B, Blattner M, Dalfovo D, Chakravarty D, Soong D, Cotter KA, Petris G, Dhingra P, Gasperini P, Cereseto A, Elemento O, Sboner A, Khurana E, Inga A, Rubin MA, Demichelis F. Nat Commun. 2017 Jun 29;8(1):48. doi: 10.1038/s41467-017-00046-0. PubMed
Comparative analysis of chimeric ZFP-, TALE- and Cas9-piggyBac transposases for integration into a single locus in human cells. Luo W, Galvan DL, Woodard LE, Dorset D, Levy S, Wilson MH. Nucleic Acids Res. 2017 Jun 29. doi: 10.1093/nar/gkx572. PubMed
Norrin-induced Frizzled4 endocytosis and endo-lysosomal trafficking control retinal angiogenesis and barrier function. Zhang C, Lai MB, Khandan L, Lee LA, Chen Z, Junge HJ. Nat Commun. 2017 Jul 4;8:16050. doi: 10.1038/ncomms16050. PubMed
A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid. Talas A, Kulcsar PI, Weinhardt N, Borsy A, Toth E, Szebenyi K, Krausz SL, Huszar K, Vida I, Sturm A, Gordos B, Hoffmann OI, Bencsura P, Nyeste A, Ligeti Z, Fodor E, Welker E. DNA Res. 2017 Jun 30. doi: 10.1093/dnares/dsx029. PubMed

Associated Plasmids

HSF1 and HSF3 cooperatively regulate the heat shock response in lizards. Takii R, Fujimoto M, Matsuura Y, Wu F, Oshibe N, Takaki E, Katiyar A, Akashi H, Makino T, Kawata M, Nakai A. PLoS One. 2017 Jul 7;12(7):e0180776. doi: 10.1371/journal.pone.0180776. eCollection 2017. PubMed
Somatic chromosomal engineering identifies BCAN-NTRK1 as a potent glioma driver and therapeutic target. Cook PJ, Thomas R, Kannan R, de Leon ES, Drilon A, Rosenblum MK, Scaltriti M, Benezra R, Ventura A. Nat Commun. 2017 Jul 11;8:15987. doi: 10.1038/ncomms15987. PubMed

Associated Plasmids

Dual loss of human POLQ and LIG4 abolishes random integration. Saito S, Maeda R, Adachi N. Nat Commun. 2017 Jul 11;8:16112. doi: 10.1038/ncomms16112. PubMed
Putative binding sites for mir-125 family miRNAs in the mouse Lfng 3'UTR affect transcript expression in the segmentation clock, but mir-125a-5p is dispensable for normal somitogenesis. Wahi K, Friesen S, Coppola V, Cole SE. Dev Dyn. 2017 Oct;246(10):740-748. doi: 10.1002/dvdy.24552. Epub 2017 Aug 18. PubMed
Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria. Vukotic M, Nolte H, Konig T, Saita S, Ananjew M, Kruger M, Tatsuta T, Langer T. Mol Cell. 2017 Aug 3;67(3):471-483.e7. doi: 10.1016/j.molcel.2017.06.013. Epub 2017 Jul 14. PubMed
Protein kinase D at the Golgi controls NLRP3 inflammasome activation. Zhang Z, Meszaros G, He WT, Xu Y, de Fatima Magliarelli H, Mailly L, Mihlan M, Liu Y, Puig Gamez M, Goginashvili A, Pasquier A, Bielska O, Neven B, Quartier P, Aebersold R, Baumert TF, Georgel P, Han J, Ricci R. J Exp Med. 2017 Sep 4;214(9):2671-2693. doi: 10.1084/jem.20162040. Epub 2017 Jul 17. PubMed
A BAG3 chaperone complex maintains cardiomyocyte function during proteotoxic stress. Judge LM, Perez-Bermejo JA, Truong A, Ribeiro AJ, Yoo JC, Jensen CL, Mandegar MA, Huebsch N, Kaake RM, So PL, Srivastava D, Pruitt BL, Krogan NJ, Conklin BR. JCI Insight. 2017 Jul 20;2(14). pii: 94623. doi: 10.1172/jci.insight.94623. eCollection 2017 Jul 20. PubMed
Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells. Royba E, Miyamoto T, Natsuko Akutsu S, Hosoba K, Tauchi H, Kudo Y, Tashiro S, Yamamoto T, Matsuura S. Sci Rep. 2017 Jul 20;7(1):5996. doi: 10.1038/s41598-017-06393-8. PubMed
Tissue-specific CTCF-cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo. Hanssen LLP, Kassouf MT, Oudelaar AM, Biggs D, Preece C, Downes DJ, Gosden M, Sharpe JA, Sloane-Stanley JA, Hughes JR, Davies B, Higgs DR. Nat Cell Biol. 2017 Aug;19(8):952-961. doi: 10.1038/ncb3573. Epub 2017 Jul 24. PubMed
Hepatitis B virus prevents excessive viral production via reduction of cell death-inducing DFF45-like effectors. Yasumoto J, Kasai H, Yoshimura K, Otoguro T, Watashi K, Wakita T, Yamashita A, Tanaka T, Takeda S, Moriishi K. J Gen Virol. 2017 Jul;98(7):1762-1773. doi: 10.1099/jgv.0.000813. PubMed
Echinomycin inhibits adipogenesis in 3T3-L1 cells in a HIF-independent manner. Yamaguchi J, Tanaka T, Saito H, Nomura S, Aburatani H, Waki H, Kadowaki T, Nangaku M. Sci Rep. 2017 Jul 26;7(1):6516. doi: 10.1038/s41598-017-06761-4. PubMed
GA-Binding Protein Alpha Is Involved in the Survival of Mouse Embryonic Stem Cells. Ueda A, Akagi T, Yokota T. Stem Cells. 2017 Nov;35(11):2229-2238. doi: 10.1002/stem.2673. Epub 2017 Aug 8. PubMed
Immunocytochemistry and fluorescence imaging efficiently identify individual neurons with CRISPR/Cas9-mediated gene disruption in primary cortical cultures. Tsunematsu H, Uyeda A, Yamamoto N, Sugo N. BMC Neurosci. 2017 Aug 1;18(1):55. doi: 10.1186/s12868-017-0377-9. PubMed
Reactivity of human AGO2 monoclonal antibody 11A9 with the SWI/SNF complex: A case study for rigorously defining antibody selectivity. van Eijl RAPM, van den Brand T, Nguyen LN, Mulder KW. Sci Rep. 2017 Aug 4;7(1):7278. doi: 10.1038/s41598-017-07539-4. PubMed
Trap(Seq): An RNA Sequencing-Based Pipeline for the Identification of Gene-Trap Insertions in Mammalian Cells. Mayor-Ruiz C, Dominguez O, Fernandez-Capetillo O. J Mol Biol. 2017 Sep 1;429(18):2780-2789. doi: 10.1016/j.jmb.2017.07.020. Epub 2017 Aug 4. PubMed
Early and consistent overexpression of ADRM1 in ovarian high-grade serous carcinoma. Jiang RT, Yemelyanova A, Xing D, Anchoori RK, Hamazaki J, Murata S, Seidman JD, Wang TL, Roden RBS. J Ovarian Res. 2017 Aug 7;10(1):53. doi: 10.1186/s13048-017-0347-y. PubMed
Atg2A/B deficiency switches cytoprotective autophagy to non-canonical caspase-8 activation and apoptosis. Tang Z, Takahashi Y, Chen C, Liu Y, He H, Tsotakos N, Serfass JM, Gebru MT, Chen H, Young MM, Wang HG. Cell Death Differ. 2017 Dec;24(12):2127-2138. doi: 10.1038/cdd.2017.133. Epub 2017 Aug 11. PubMed
Reck and Gpr124 Are Essential Receptor Cofactors for Wnt7a/Wnt7b-Specific Signaling in Mammalian CNS Angiogenesis and Blood-Brain Barrier Regulation. Cho C, Smallwood PM, Nathans J. Neuron. 2017 Aug 30;95(5):1056-1073.e5. doi: 10.1016/j.neuron.2017.07.031. Epub 2017 Aug 10. PubMed
Listeria monocytogenes and Shigella flexneri Activate the NLRP1B Inflammasome. Neiman-Zenevich J, Stuart S, Abdel-Nour M, Girardin SE, Mogridge J. Infect Immun. 2017 Oct 18;85(11). pii: IAI.00338-17. doi: 10.1128/IAI.00338-17. Print 2017 Nov. PubMed
Identification of CMTM6 and CMTM4 as PD-L1 protein regulators. Mezzadra R, Sun C, Jae LT, Gomez-Eerland R, de Vries E, Wu W, Logtenberg MEW, Slagter M, Rozeman EA, Hofland I, Broeks A, Horlings HM, Wessels LFA, Blank CU, Xiao Y, Heck AJR, Borst J, Brummelkamp TR, Schumacher TNM. Nature. 2017 Sep 7;549(7670):106-110. doi: 10.1038/nature23669. Epub 2017 Aug 16. PubMed
ADAM10-Mediated ICOS Ligand Shedding on B Cells Is Necessary for Proper T Cell ICOS Regulation and T Follicular Helper Responses. Lownik JC, Luker AJ, Damle SR, Cooley LF, El Sayed R, Hutloff A, Pitzalis C, Martin RK, El Shikh MEM, Conrad DH. J Immunol. 2017 Oct 1;199(7):2305-2315. doi: 10.4049/jimmunol.1700833. Epub 2017 Aug 16. PubMed
Small molecules enhance CRISPR/Cas9-mediated homology-directed genome editing in primary cells. Li G, Zhang X, Zhong C, Mo J, Quan R, Yang J, Liu D, Li Z, Yang H, Wu Z. Sci Rep. 2017 Aug 21;7(1):8943. doi: 10.1038/s41598-017-09306-x. PubMed
B-cell Receptor Signaling Regulates Metabolism in Chronic Lymphocytic Leukemia. Vangapandu HV, Havranek O, Ayres ML, Kaipparettu BA, Balakrishnan K, Wierda WG, Keating MJ, Davis RE, Stellrecht CM, Gandhi V. Mol Cancer Res. 2017 Dec;15(12):1692-1703. doi: 10.1158/1541-7786.MCR-17-0026. Epub 2017 Aug 23. PubMed
Functional significance of rare neuroligin 1 variants found in autism. Nakanishi M, Nomura J, Ji X, Tamada K, Arai T, Takahashi E, Bucan M, Takumi T. PLoS Genet. 2017 Aug 25;13(8):e1006940. doi: 10.1371/journal.pgen.1006940. eCollection 2017 Aug. PubMed
Esco1 and Esco2 regulate distinct cohesin functions during cell cycle progression. Alomer RM, da Silva EML, Chen J, Piekarz KM, McDonald K, Sansam CG, Sansam CL, Rankin S. Proc Natl Acad Sci U S A. 2017 Sep 12;114(37):9906-9911. doi: 10.1073/pnas.1708291114. Epub 2017 Aug 28. PubMed
Multiplexed sgRNA Expression Allows Versatile Single Nonrepetitive DNA Labeling and Endogenous Gene Regulation. Shao S, Chang L, Sun Y, Hou Y, Fan X, Sun Y. ACS Synth Biol. 2017 Sep 7. doi: 10.1021/acssynbio.7b00268. PubMed

Associated Plasmids

Postnatal Cardiac Gene Editing Using CRISPR/Cas9 With AAV9-Mediated Delivery of Short Guide RNAs Results in Mosaic Gene Disruption. Johansen AK, Molenaar B, Versteeg D, Leitoguinho AR, Demkes C, Spanjaard B, de Ruiter H, Akbari Moqadam F, Kooijman L, Zentilin L, Giacca M, van Rooij E. Circ Res. 2017 Oct 27;121(10):1168-1181. doi: 10.1161/CIRCRESAHA.116.310370. Epub 2017 Aug 29. PubMed

Associated Plasmids

Deficiency in Kelch protein Klhl31 causes congenital myopathy in mice. Papizan JB, Garry GA, Brezprozvannaya S, McAnally JR, Bassel-Duby R, Liu N, Olson EN. J Clin Invest. 2017 Oct 2;127(10):3730-3740. doi: 10.1172/JCI93445. Epub 2017 Sep 5. PubMed
Haploid Mammalian Genetic Screen Identifies UBXD8 as a Key Determinant of HMGCR Degradation and Cholesterol Biosynthesis. Loregger A, Raaben M, Tan J, Scheij S, Moeton M, van den Berg M, Gelberg-Etel H, Stickel E, Roitelman J, Brummelkamp T, Zelcer N. Arterioscler Thromb Vasc Biol. 2017 Nov;37(11):2064-2074. doi: 10.1161/ATVBAHA.117.310002. Epub 2017 Sep 7. PubMed
Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2. Tungadi EA, Ito A, Kiyomitsu T, Goshima G. J Cell Sci. 2017 Nov 1;130(21):3676-3684. doi: 10.1242/jcs.203703. Epub 2017 Sep 7. PubMed
Distinct roles of neuronal and microglial CB2 cannabinoid receptors in the mouse hippocampus. Li Y, Kim J. Neuroscience. 2017 Sep 6. pii: S0306-4522(17)30629-2. doi: 10.1016/j.neuroscience.2017.08.053. PubMed

Associated Plasmids

CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11. PubMed
Prion protein is required for tumor necrosis factor alpha (TNFalpha)-triggered nuclear factor kappaB (NF-kappaB) signaling and cytokine production. Wu GR, Mu TC, Gao ZX, Wang J, Sy MS, Li CY. J Biol Chem. 2017 Nov 17;292(46):18747-18759. doi: 10.1074/jbc.M117.787283. Epub 2017 Sep 12. PubMed
Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRgamma-mediated phagocytosis and chemokine expression in U937 cells. Chihara K, Kato Y, Yoshiki H, Takeuchi K, Fujieda S, Sada K. Sci Rep. 2017 Sep 13;7(1):11480. doi: 10.1038/s41598-017-11915-5. PubMed
Centriole triplet microtubules are required for stable centriole formation and inheritance in human cells. Wang JT, Kong D, Hoerner CR, Loncarek J, Stearns T. Elife. 2017 Sep 14;6. pii: 29061. doi: 10.7554/eLife.29061. PubMed
Massive reshaping of genome-nuclear lamina interactions during oncogene-induced senescence. Lenain C, de Graaf CA, Pagie L, Visser NL, de Haas M, de Vries SS, Peric-Hupkes D, van Steensel B, Peeper DS. Genome Res. 2017 Oct;27(10):1634-1644. doi: 10.1101/gr.225763.117. Epub 2017 Sep 15. PubMed
Efficient gene editing via non-viral delivery of CRISPR-Cas9 system using polymeric and hybrid microcarriers. Timin AS, Muslimov AR, Lepik KV, Epifanovskaya OS, Shakirova AI, Mock U, Riecken K, Okilova MV, Sergeev VS, Afanasyev BV, Fehse B, Sukhorukov GB. Nanomedicine. 2018 Jan;14(1):97-108. doi: 10.1016/j.nano.2017.09.001. Epub 2017 Sep 14. PubMed
CRISPR/Cas9 Engineering of Adult Mouse Liver Demonstrates That the Dnajb1-Prkaca Gene Fusion Is Sufficient to Induce Tumors Resembling Fibrolamellar Hepatocellular Carcinoma. Engelholm LH, Riaz A, Serra D, Dagnaes-Hansen F, Johansen JV, Santoni-Rugiu E, Hansen SH, Niola F, Frodin M. Gastroenterology. 2017 Dec;153(6):1662-1673.e10. doi: 10.1053/j.gastro.2017.09.008. Epub 2017 Sep 18. PubMed
Immunosurveillance and Immunoediting of Breast Cancer via Class I MHC Receptors. Tu MM, Rahim MMA, Sayed C, Mahmoud AB, Makrigiannis AP. Cancer Immunol Res. 2017 Nov;5(11):1016-1028. doi: 10.1158/2326-6066.CIR-17-0056. Epub 2017 Sep 18. PubMed
In vivo mouse and live cell STED microscopy of neuronal actin plasticity using far-red emitting fluorescent proteins. Wegner W, Ilgen P, Gregor C, van Dort J, Mott AC, Steffens H, Willig KI. Sci Rep. 2017 Sep 18;7(1):11781. doi: 10.1038/s41598-017-11827-4. PubMed
Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9. Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T. Stem Cell Res. 2017 Aug;23:105-108. doi: 10.1016/j.scr.2017.07.006. Epub 2017 Jul 11. PubMed

Associated Plasmids

Enhancing CRISPR/Cas9-mediated homology-directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52. Shao S, Ren C, Liu Z, Bai Y, Chen Z, Wei Z, Wang X, Zhang Z, Xu K. Int J Biochem Cell Biol. 2017 Nov;92:43-52. doi: 10.1016/j.biocel.2017.09.012. Epub 2017 Sep 18. PubMed
Correction of beta-thalassemia mutant by base editor in human embryos. Liang P, Ding C, Sun H, Xie X, Xu Y, Zhang X, Sun Y, Xiong Y, Ma W, Liu Y, Wang Y, Fang J, Liu D, Songyang Z, Zhou C, Huang J. Protein Cell. 2017 Nov;8(11):811-822. doi: 10.1007/s13238-017-0475-6. Epub 2017 Sep 23. PubMed
Generation of a novel Antibody-Drug Conjugate targeting endosialin: potent and durable antitumor response in sarcoma. Capone E, Piccolo E, Fichera I, Ciufici P, Barcaroli D, Sala A, De Laurenzi V, Iacobelli V, Iacobelli S, Sala G. Oncotarget. 2017 Jul 22;8(36):60368-60377. doi: 10.18632/oncotarget.19499. eCollection 2017 Sep 1. PubMed
Genome editing reveals a role for OCT4 in human embryogenesis. Fogarty NME, McCarthy A, Snijders KE, Powell BE, Kubikova N, Blakeley P, Lea R, Elder K, Wamaitha SE, Kim D, Maciulyte V, Kleinjung J, Kim JS, Wells D, Vallier L, Bertero A, Turner JMA, Niakan KK. Nature. 2017 Oct 5;550(7674):67-73. doi: 10.1038/nature24033. Epub 2017 Sep 20. PubMed
Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN. Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J. Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023. PubMed

Associated Plasmids

Nesprin-1alpha-Dependent Microtubule Nucleation from the Nuclear Envelope via Akap450 Is Necessary for Nuclear Positioning in Muscle Cells. Gimpel P, Lee YL, Sobota RM, Calvi A, Koullourou V, Patel R, Mamchaoui K, Nedelec F, Shackleton S, Schmoranzer J, Burke B, Cadot B, Gomes ER. Curr Biol. 2017 Oct 9;27(19):2999-3009.e9. doi: 10.1016/j.cub.2017.08.031. Epub 2017 Sep 28. PubMed
Histone H3K4 monomethylation catalyzed by Trr and mammalian COMPASS-like proteins at enhancers is dispensable for development and viability. Rickels R, Herz HM, Sze CC, Cao K, Morgan MA, Collings CK, Gause M, Takahashi YH, Wang L, Rendleman EJ, Marshall SA, Krueger A, Bartom ET, Piunti A, Smith ER, Abshiru NA, Kelleher NL, Dorsett D, Shilatifard A. Nat Genet. 2017 Nov;49(11):1647-1653. doi: 10.1038/ng.3965. Epub 2017 Oct 2. PubMed
t-BuOOH induces ferroptosis in human and murine cell lines. Wenz C, Faust D, Linz B, Turmann C, Nikolova T, Bertin J, Gough P, Wipf P, Schroder AS, Krautwald S, Dietrich C. Arch Toxicol. 2018 Feb;92(2):759-775. doi: 10.1007/s00204-017-2066-y. Epub 2017 Oct 3. PubMed
H2AX facilitates classical non-homologous end joining at the expense of limited nucleotide loss at repair junctions. Feng YL, Xiang JF, Liu SC, Guo T, Yan GF, Feng Y, Kong N, Li HD, Huang Y, Lin H, Cai XJ, Xie AY. Nucleic Acids Res. 2017 Oct 13;45(18):10614-10633. doi: 10.1093/nar/gkx715. PubMed
RUNX2 expression in thyroid and breast cancer requires the cooperation of three non-redundant enhancers under the control of BRD4 and c-JUN. Sancisi V, Manzotti G, Gugnoni M, Rossi T, Gandolfi G, Gobbi G, Torricelli F, Catellani F, Faria do Valle I, Remondini D, Castellani G, Ragazzi M, Piana S, Ciarrocchi A. Nucleic Acids Res. 2017 Nov 2;45(19):11249-11267. doi: 10.1093/nar/gkx802. PubMed
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. PubMed

Associated Plasmids

CRISPR/Cas9-induced transgene insertion and telomere-associated truncation of a single human chromosome for chromosome engineering in CHO and A9 cells. Uno N, Hiramatsu K, Uno K, Komoto S, Kazuki Y, Oshimura M. Sci Rep. 2017 Oct 6;7(1):12739. doi: 10.1038/s41598-017-10418-7. PubMed
CRISPR/Cas9-mediated knockout of myostatin in Chinese indigenous Erhualian pigs. Wang K, Tang X, Xie Z, Zou X, Li M, Yuan H, Guo N, Ouyang H, Jiao H, Pang D. Transgenic Res. 2017 Dec;26(6):799-805. doi: 10.1007/s11248-017-0044-z. Epub 2017 Oct 9. PubMed
A point mutation in the ion conduction pore of AMPA receptor GRIA3 causes dramatically perturbed sleep patterns as well as intellectual disability. Davies B, Brown LA, Cais O, Watson J, Clayton AJ, Chang VT, Biggs D, Preece C, Hernandez-Pliego P, Krohn J, Bhomra A, Twigg SRF, Rimmer A, Kanapin A, Sen A, Zaiwalla Z, McVean G, Foster R, Donnelly P, Taylor JC, Blair E, Nutt D, Aricescu AR, Greger IH, Peirson SN, Flint J, Martin HC. Hum Mol Genet. 2017 Oct 15;26(20):3869-3882. doi: 10.1093/hmg/ddx270. PubMed
Radically truncated MeCP2 rescues Rett syndrome-like neurological defects. Tillotson R, Selfridge J, Koerner MV, Gadalla KKE, Guy J, De Sousa D, Hector RD, Cobb SR, Bird A. Nature. 2017 Oct 19;550(7676):398-401. doi: 10.1038/nature24058. Epub 2017 Oct 11. PubMed

Associated Plasmids

Diverse Viruses Require the Calcium Transporter SPCA1 for Maturation and Spread. Hoffmann HH, Schneider WM, Blomen VA, Scull MA, Hovnanian A, Brummelkamp TR, Rice CM. Cell Host Microbe. 2017 Oct 11;22(4):460-470.e5. doi: 10.1016/j.chom.2017.09.002. PubMed
A long-range cis-regulatory element for class I odorant receptor genes. Iwata T, Niimura Y, Kobayashi C, Shirakawa D, Suzuki H, Enomoto T, Touhara K, Yoshihara Y, Hirota J. Nat Commun. 2017 Oct 12;8(1):885. doi: 10.1038/s41467-017-00870-4. PubMed
Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux. Vaites LP, Paulo JA, Huttlin EL, Harper JW. Mol Cell Biol. 2017 Dec 13;38(1). pii: MCB.00392-17. doi: 10.1128/MCB.00392-17. Print 2018 Jan 1. PubMed
Control of growth and gut maturation by HoxD genes and the associated lncRNA Haglr. Zakany J, Darbellay F, Mascrez B, Necsulea A, Duboule D. Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):E9290-E9299. doi: 10.1073/pnas.1712511114. Epub 2017 Oct 17. PubMed
Polycomb group RING finger proteins 3/5 activate transcription via an interaction with the pluripotency factor Tex10 in embryonic stem cells. Zhao W, Huang Y, Zhang J, Liu M, Ji H, Wang C, Cao N, Li C, Xia Y, Jiang Q, Qin J. J Biol Chem. 2017 Dec 29;292(52):21527-21537. doi: 10.1074/jbc.M117.804054. Epub 2017 Oct 20. PubMed
Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models. Fujihara Y, Oji A, Larasati T, Kojima-Kita K, Ikawa M. Int J Mol Sci. 2017 Oct 21;18(10). pii: ijms18102208. doi: 10.3390/ijms18102208. PubMed
In Vivo Translatome Profiling in Spinal Muscular Atrophy Reveals a Role for SMN Protein in Ribosome Biology. Bernabo P, Tebaldi T, Groen EJN, Lane FM, Perenthaler E, Mattedi F, Newbery HJ, Zhou H, Zuccotti P, Potrich V, Shorrock HK, Muntoni F, Quattrone A, Gillingwater TH, Viero G. Cell Rep. 2017 Oct 24;21(4):953-965. doi: 10.1016/j.celrep.2017.10.010. PubMed
Baf53a is involved in survival of mouse ES cells, which can be compensated by Baf53b. Zhu B, Ueda A, Song X, Horike SI, Yokota T, Akagi T. Sci Rep. 2017 Oct 25;7(1):14059. doi: 10.1038/s41598-017-14362-4. PubMed
PAXX and Xlf interplay revealed by impaired CNS development and immunodeficiency of double KO mice. Abramowski V, Etienne O, Elsaid R, Yang J, Berland A, Kermasson L, Roch B, Musilli S, Moussu JP, Lipson-Ruffert K, Revy P, Cumano A, Boussin FD, de Villartay JP. Cell Death Differ. 2018 Feb;25(2):444-452. doi: 10.1038/cdd.2017.184. Epub 2017 Oct 27. PubMed
APELA promotes tumour growth and cell migration in ovarian cancer in a p53-dependent manner. Yi Y, Tsai SH, Cheng JC, Wang EY, Anglesio MS, Cochrane DR, Fuller M, Gibb EA, Wei W, Huntsman DG, Karsan A, Hoodless PA. Gynecol Oncol. 2017 Dec;147(3):663-671. doi: 10.1016/j.ygyno.2017.10.016. Epub 2017 Oct 24. PubMed
Integrated Proteomic and Transcriptomic Analysis Reveals Long Noncoding RNA HOX Transcript Antisense Intergenic RNA (HOTAIR) Promotes Hepatocellular Carcinoma Cell Proliferation by Regulating Opioid Growth Factor Receptor (OGFr). Wu Y, Xiong Q, Li S, Yang X, Ge F. Mol Cell Proteomics. 2018 Jan;17(1):146-159. doi: 10.1074/mcp.RA117.000277. Epub 2017 Oct 27. PubMed
Murine pluripotent stem cells with a homozygous knockout of Foxg1 show reduced differentiation towards cortical progenitors in vitro. Mall EM, Herrmann D, Niemann H. Stem Cell Res. 2017 Dec;25:50-60. doi: 10.1016/j.scr.2017.10.012. Epub 2017 Oct 19. PubMed
A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation. Morgan MAJ, Rickels RA, Collings CK, He X, Cao K, Herz HM, Cozzolino KA, Abshiru NA, Marshall SA, Rendleman EJ, Sze CC, Piunti A, Kelleher NL, Savas JN, Shilatifard A. Genes Dev. 2017 Oct 1;31(19):2003-2014. doi: 10.1101/gad.305201.117. PubMed
Modification of single-nucleotide polymorphism in a fully humanized CYP3A mouse by genome editing technology. Abe S, Kobayashi K, Oji A, Sakuma T, Kazuki K, Takehara S, Nakamura K, Okada A, Tsukazaki Y, Senda N, Honma K, Yamamoto T, Ikawa M, Chiba K, Oshimura M, Kazuki Y. Sci Rep. 2017 Nov 9;7(1):15189. doi: 10.1038/s41598-017-15033-0. PubMed
Distinct functional consequences of ECEL1/DINE missense mutations in the pathogenesis of congenital contracture disorders. Nagata K, Takahashi M, Kiryu-Seo S, Kiyama H, Saido TC. Acta Neuropathol Commun. 2017 Nov 13;5(1):83. doi: 10.1186/s40478-017-0486-9. PubMed
Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mitophagy Pathway. Le Guerroue F, Eck F, Jung J, Starzetz T, Mittelbronn M, Kaulich M, Behrends C. Mol Cell. 2017 Nov 16;68(4):786-796.e6. doi: 10.1016/j.molcel.2017.10.029. PubMed
The role of N-terminal segment and membrane association in MyD88-mediated signaling. Avbelj M, Panter G, Jerala R. Biochem Biophys Res Commun. 2018 Jan 1;495(1):878-883. doi: 10.1016/j.bbrc.2017.11.099. Epub 2017 Nov 16. PubMed
Histone methyltransferase MMSET promotes AID-mediated DNA breaks at the donor switch region during class switch recombination. Nguyen HV, Dong J, Panchakshari RA, Kumar V, Alt FW, Bories JC. Proc Natl Acad Sci U S A. 2017 Dec 5;114(49):E10560-E10567. doi: 10.1073/pnas.1701366114. Epub 2017 Nov 20. PubMed
Crispr-Cas9 engineered osteogenesis imperfecta type V leads to severe skeletal deformities and perinatal lethality in mice. Rauch F, Geng Y, Lamplugh L, Hekmatnejad B, Gaumond MH, Penney J, Yamanaka Y, Moffatt P. Bone. 2018 Feb;107:131-142. doi: 10.1016/j.bone.2017.11.013. Epub 2017 Nov 22. PubMed
CRISPR/Cas9-mediated genome editing in naive human embryonic stem cells. Jacobs EZ, Warrier S, Volders PJ, D'haene E, Van Lombergen E, Vantomme L, Van der Jeught M, Heindryckx B, Menten B, Vergult S. Sci Rep. 2017 Nov 30;7(1):16650. doi: 10.1038/s41598-017-16932-y. PubMed
The Pore-Forming Protein Gasdermin D Regulates Interleukin-1 Secretion from Living Macrophages. Evavold CL, Ruan J, Tan Y, Xia S, Wu H, Kagan JC. Immunity. 2018 Jan 16;48(1):35-44.e6. doi: 10.1016/j.immuni.2017.11.013. Epub 2017 Nov 28. PubMed
Transcriptional landscape of a RET(C634Y)-mutated iPSC and its CRISPR-corrected isogenic control reveals the putative role of EGR1 transcriptional program in the development of multiple endocrine neoplasia type 2A-associated cancers. Hadoux J, Desterke C, Feraud O, Guibert M, De Rose RF, Opolon P, Divers D, Gobbo E, Griscelli F, Schlumberger M, Bennaceur-Griscelli A, Turhan AG. Stem Cell Res. 2018 Jan;26:8-16. doi: 10.1016/j.scr.2017.11.015. Epub 2017 Nov 23. PubMed
Linear Integration of ERK Activity Predominates over Persistence Detection in Fra-1 Regulation. Gillies TE, Pargett M, Minguet M, Davies AE, Albeck JG. Cell Syst. 2017 Dec 27;5(6):549-563.e5. doi: 10.1016/j.cels.2017.10.019. Epub 2017 Nov 29. PubMed
CRISPR/Cas9 Editing of the Mouse Thra Gene Produces Models with Variable Resistance to Thyroid Hormone. Markossian S, Guyot R, Richard S, Teixeira M, Aguilera N, Bouchet M, Plateroti M, Guan W, Gauthier K, Aubert D, Flamant F. Thyroid. 2018 Jan;28(1):139-150. doi: 10.1089/thy.2017.0389. Epub 2018 Jan 2. PubMed
A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3alpha and GSK3beta in Regulating Embryonic Stem Cell Fate. Chen X, Wang R, Liu X, Wu Y, Zhou T, Yang Y, Perez A, Chen YC, Hu L, Chadarevian JP, Assadieskandar A, Zhang C, Ying QL. Dev Cell. 2017 Dec 4;43(5):563-576.e4. doi: 10.1016/j.devcel.2017.11.007. PubMed
Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. Adikusuma F, Pfitzner C, Thomas PQ. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. PubMed

Associated Plasmids

PRPF8 is important for BRCA1-mediated homologous recombination. Onyango DO, Lee G, Stark JM. Oncotarget. 2017 Oct 6;8(55):93319-93337. doi: 10.18632/oncotarget.21555. eCollection 2017 Nov 7. PubMed
A novel mouse model for tracking the fate of CXCR5-expressing T cells. Takebe T, Sakamoto K, Higami Y, Harada Y. Biochem Biophys Res Commun. 2018 Jan 8;495(2):1642-1647. doi: 10.1016/j.bbrc.2017.12.029. Epub 2017 Dec 6. PubMed
EGFR feedback-inhibition by Ran-binding protein 6 is disrupted in cancer. Oldrini B, Hsieh WY, Erdjument-Bromage H, Codega P, Carro MS, Curiel-Garcia A, Campos C, Pourmaleki M, Grommes C, Vivanco I, Rohle D, Bielski CM, Taylor BS, Hollmann TJ, Rosenblum M, Tempst P, Blenis J, Squatrito M, Mellinghoff IK. Nat Commun. 2017 Dec 11;8(1):2035. doi: 10.1038/s41467-017-02185-w. PubMed
Regulation of type I collagen expression by microRNA-29 following ionizing radiation. Yano H, Hamanaka R, Nakamura-Ota M, Zhang JJ, Matsuo N, Yoshioka H. Radiat Environ Biophys. 2018 Mar;57(1):41-54. doi: 10.1007/s00411-017-0723-4. Epub 2017 Dec 11. PubMed
Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing. Lu J, Zhao C, Zhao Y, Zhang J, Zhang Y, Chen L, Han Q, Ying Y, Peng S, Ai R, Wang Y. Nucleic Acids Res. 2018 Mar 16;46(5):e25. doi: 10.1093/nar/gkx1222. PubMed

Associated Plasmids

SaCas9 Requires 5'-NNGRRT-3' PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells. Xie H, Tang L, He X, Liu X, Zhou C, Liu J, Ge X, Li J, Liu C, Zhao J, Qu J, Song Z, Gu F. Biotechnol J. 2018 Apr;13(4):e1700561. doi: 10.1002/biot.201700561. Epub 2018 Jan 10. PubMed
Site-specific chromosomal gene insertion: Flp recombinase versus Cas9 nuclease. Phan QV, Contzen J, Seemann P, Gossen M. Sci Rep. 2017 Dec 19;7(1):17771. doi: 10.1038/s41598-017-17651-0. PubMed
OTX1 regulates cell cycle progression of neural progenitors in the developing cerebral cortex. Huang B, Li X, Tu X, Zhao W, Zhu D, Feng Y, Si X, Chen JG. J Biol Chem. 2018 Feb 9;293(6):2137-2148. doi: 10.1074/jbc.RA117.001249. Epub 2017 Dec 22. PubMed
Serine/threonine phosphatase 5 (PP5C/PPP5C) regulates the ISOC channel through a PP5C-FKBP51 axis. Hamilton CL, Abney KA, Vasauskas AA, Alexeyev M, Li N, Honkanen RE, Scammell JG, Cioffi DL. Pulm Circ. 2018 Jan-Mar;8(1):2045893217753156. doi: 10.1177/2045893217753156. Epub 2017 Dec 28. PubMed
Reconstruction of the Human Colon Epithelium In Vivo. Sugimoto S, Ohta Y, Fujii M, Matano M, Shimokawa M, Nanki K, Date S, Nishikori S, Nakazato Y, Nakamura T, Kanai T, Sato T. Cell Stem Cell. 2018 Feb 1;22(2):171-176.e5. doi: 10.1016/j.stem.2017.11.012. Epub 2017 Dec 28. PubMed
Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9-mediated ablation. Chang CW, Chang CC, Hsia KC, Tsai SY. Stem Cell Res. 2018 Mar;27:21-24. doi: 10.1016/j.scr.2017.12.015. Epub 2017 Dec 23. PubMed
Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems. Kawabe Y, Komatsu S, Komatsu S, Murakami M, Ito A, Sakuma T, Nakamura T, Yamamoto T, Kamihira M. J Biosci Bioeng. 2018 May;125(5):599-605. doi: 10.1016/j.jbiosc.2017.12.003. Epub 2017 Dec 30. PubMed
Lamp1 Increases the Efficiency of Lassa Virus Infection by Promoting Fusion in Less Acidic Endosomal Compartments. Hulseberg CE, Feneant L, Szymanska KM, White JM. MBio. 2018 Jan 2;9(1). pii: mBio.01818-17. doi: 10.1128/mBio.01818-17. PubMed
Gene correction of HAX1 reversed Kostmann disease phenotype in patient-specific induced pluripotent stem cells. Pittermann E, Lachmann N, MacLean G, Emmrich S, Ackermann M, Gohring G, Schlegelberger B, Welte K, Schambach A, Heckl D, Orkin SH, Cantz T, Klusmann JH. Blood Adv. 2017 Jun 2;1(14):903-914. doi: 10.1182/bloodadvances.2016003798. eCollection 2017 Jun 13. PubMed
A knock-in mouse strain facilitates dynamic tracking and enrichment of MEIS1. Xiang P, Wei W, Hofs N, Clemans-Gibbon J, Maetzig T, Lai CK, Dhillon I, May C, Ruschmann J, Schneider E, Rosten P, Hu K, Kuchenbauer F, Hoodless PA, Humphries RK. Blood Adv. 2017 Nov 7;1(24):2225-2235. doi: 10.1182/bloodadvances.2017010355. eCollection 2017 Nov 14. PubMed
Abnormal Cell Sorting Underlies the Unique X-Linked Inheritance of PCDH19 Epilepsy. Pederick DT, Richards KL, Piltz SG, Kumar R, Mincheva-Tasheva S, Mandelstam SA, Dale RC, Scheffer IE, Gecz J, Petrou S, Hughes JN, Thomas PQ. Neuron. 2018 Jan 3;97(1):59-66.e5. doi: 10.1016/j.neuron.2017.12.005. PubMed
DNA double-strand break response factors influence end-joining features of IgH class switch and general translocation junctions. Panchakshari RA, Zhang X, Kumar V, Du Z, Wei PC, Kao J, Dong J, Alt FW. Proc Natl Acad Sci U S A. 2018 Jan 23;115(4):762-767. doi: 10.1073/pnas.1719988115. Epub 2018 Jan 8. PubMed
SCFAs strongly stimulate PYY production in human enteroendocrine cells. Larraufie P, Martin-Gallausiaux C, Lapaque N, Dore J, Gribble FM, Reimann F, Blottiere HM. Sci Rep. 2018 Jan 8;8(1):74. doi: 10.1038/s41598-017-18259-0. PubMed
TIP60 Complex Inhibits Hepatitis B Virus Transcription. Nishitsuji H, Ujino S, Harada K, Shimotohno K. J Virol. 2018 Feb 26;92(6). pii: JVI.01788-17. doi: 10.1128/JVI.01788-17. Print 2018 Mar 15. PubMed
Improved Cytotoxic T Lymphocyte Responses to Vaccination with Porcine Reproductive and Respiratory Syndrome Virus in 4-1BB Transgenic Pigs. Huang G, Liu X, Duszynski DW, Tang X, El-Ashram S, Liu Z, Suo X, Li Q. Front Immunol. 2017 Dec 18;8:1846. doi: 10.3389/fimmu.2017.01846. eCollection 2017. PubMed
Generation of Optogenetically Modified Adenovirus Vector for Spatiotemporally Controllable Gene Therapy. Takayama K, Mizuguchi H. ACS Chem Biol. 2018 Feb 16;13(2):449-454. doi: 10.1021/acschembio.7b01058. Epub 2018 Jan 12. PubMed
EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation. Silva J, Aivio S, Knobel PA, Bailey LJ, Casali A, Vinaixa M, Garcia-Cao I, Coyaud E, Jourdain AA, Perez-Ferreros P, Rojas AM, Antolin-Fontes A, Samino-Gene S, Raught B, Gonzalez-Reyes A, Ribas de Pouplana L, Doherty AJ, Yanes O, Stracker TH. Nat Cell Biol. 2018 Feb;20(2):162-174. doi: 10.1038/s41556-017-0016-9. Epub 2018 Jan 15. PubMed
CD163 knockout pigs are fully resistant to highly pathogenic porcine reproductive and respiratory syndrome virus. Yang H, Zhang J, Zhang X, Shi J, Pan Y, Zhou R, Li G, Li Z, Cai G, Wu Z. Antiviral Res. 2018 Mar;151:63-70. doi: 10.1016/j.antiviral.2018.01.004. Epub 2018 Jan 11. PubMed
Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression. Seino T, Kawasaki S, Shimokawa M, Tamagawa H, Toshimitsu K, Fujii M, Ohta Y, Matano M, Nanki K, Kawasaki K, Takahashi S, Sugimoto S, Iwasaki E, Takagi J, Itoi T, Kitago M, Kitagawa Y, Kanai T, Sato T. Cell Stem Cell. 2018 Mar 1;22(3):454-467.e6. doi: 10.1016/j.stem.2017.12.009. Epub 2018 Jan 11. PubMed
Critical roles of alphaII spectrin in brain development and epileptic encephalopathy. Wang Y, Ji T, Nelson AD, Glanowska K, Murphy GG, Jenkins PM, Parent JM. J Clin Invest. 2018 Feb 1;128(2):760-773. doi: 10.1172/JCI95743. Epub 2018 Jan 16. PubMed
In Vivo Ovarian Cancer Gene Therapy Using CRISPR-Cas9. He ZY, Zhang YG, Yang YH, Ma CC, Wang P, Du W, Li L, Xiang R, Song XR, Zhao X, Yao SH, Wei YQ. Hum Gene Ther. 2018 Feb;29(2):223-233. doi: 10.1089/hum.2017.209. PubMed
Negative regulation of the Keap1-Nrf2 pathway by a p62/Sqstm1 splicing variant. Kageyama S, Saito T, Obata M, Koide RH, Ichimura Y, Komatsu M. Mol Cell Biol. 2018 Jan 16. pii: MCB.00642-17. doi: 10.1128/MCB.00642-17. PubMed
CRISPR/Cas9-mediated reversibly immortalized mouse bone marrow stromal stem cells (BMSCs) retain multipotent features of mesenchymal stem cells (MSCs). Hu X, Li L, Yu X, Zhang R, Yan S, Zeng Z, Shu Y, Zhao C, Wu X, Lei J, Li Y, Zhang W, Yang C, Wu K, Wu Y, An L, Huang S, Ji X, Gong C, Yuan C, Zhang L, Liu W, Huang B, Feng Y, Zhang B, Haydon RC, Luu HH, Reid RR, Lee MJ, Wolf JM, Yu Z, He TC. Oncotarget. 2017 Dec 5;8(67):111847-111865. doi: 10.18632/oncotarget.22915. eCollection 2017 Dec 19. PubMed
Homeostatic Response of Mouse renin Gene Transcription in a Hypertensive Environment Is Mediated by a Novel 5' Enhancer. Ushiki A, Matsuzaki H, Fukamizu A, Tanimoto K. Mol Cell Biol. 2018 Mar 15;38(7). pii: MCB.00566-17. doi: 10.1128/MCB.00566-17. Print 2018 Apr 1. PubMed
SWELL1 is a glucose sensor regulating beta-cell excitability and systemic glycaemia. Kang C, Xie L, Gunasekar SK, Mishra A, Zhang Y, Pai S, Gao Y, Kumar A, Norris AW, Stephens SB, Sah R. Nat Commun. 2018 Jan 25;9(1):367. doi: 10.1038/s41467-017-02664-0. PubMed
Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR/Cas9. Abbasi F, Miyata H, Ikawa M. Reprod Med Biol. 2017 Oct 14;17(1):3-10. doi: 10.1002/rmb2.12067. eCollection 2018 Jan. PubMed
Mitochondria-specific photoactivation to monitor local sphingosine metabolism and function. Feng S, Harayama T, Montessuit S, David FP, Winssinger N, Martinou JC, Riezman H. Elife. 2018 Jan 29;7. pii: 34555. doi: 10.7554/eLife.34555. PubMed
Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo. Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler T. Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333. PubMed

Associated Plasmids

Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease. Asano K, Suzuki T, Saito A, Wei FY, Ikeuchi Y, Numata T, Tanaka R, Yamane Y, Yamamoto T, Goto T, Kishita Y, Murayama K, Ohtake A, Okazaki Y, Tomizawa K, Sakaguchi Y, Suzuki T. Nucleic Acids Res. 2018 Feb 28;46(4):1565-1583. doi: 10.1093/nar/gky068. PubMed
R-spondins can potentiate WNT signaling without LGRs. Lebensohn AM, Rohatgi R. Elife. 2018 Feb 6;7. pii: 33126. doi: 10.7554/eLife.33126. PubMed
Reduced recruitment of 53BP1 during interstrand crosslink repair is associated with genetically inherited attenuation of mitomycin C sensitivity in a family with Fanconi anemia. Lesport E, Ferster A, Biver A, Roch B, Vasquez N, Jabado N, Vives FL, Revy P, Soulier J, de Villartay JP. Oncotarget. 2017 Dec 17;9(3):3779-3793. doi: 10.18632/oncotarget.23375. eCollection 2018 Jan 9. PubMed
Proteomic profiling of HIV-infected T-cells by SWATH mass spectrometry. DeBoer J, Wojtkiewicz MS, Haverland N, Li Y, Harwood E, Leshen E, George JW, Ciborowski P, Belshan M. Virology. 2018 Mar;516:246-257. doi: 10.1016/j.virol.2018.01.025. PubMed
RHBDD1 promotes colorectal cancer metastasis through the Wnt signaling pathway and its downstream target ZEB1. Zhang M, Miao F, Huang R, Liu W, Zhao Y, Jiao T, Lu Y, Wu F, Wang X, Wang H, Zhao H, Ju H, Miao S, Wang L, Song W. J Exp Clin Cancer Res. 2018 Feb 9;37(1):22. doi: 10.1186/s13046-018-0687-5. PubMed
CRISPR/CAS9 ablation of individual miRNAs from a miRNA family reveals their individual efficacies for regulating cardiac differentiation. Zhang Z, Ursin R, Mahapatra S, Gallicano GI. Mech Dev. 2018 Apr;150:10-20. doi: 10.1016/j.mod.2018.02.002. Epub 2018 Feb 8. PubMed
A highly specific SpCas9 variant is identified by in vivo screening in yeast. Casini A, Olivieri M, Petris G, Montagna C, Reginato G, Maule G, Lorenzin F, Prandi D, Romanel A, Demichelis F, Inga A, Cereseto A. Nat Biotechnol. 2018 Jan 29. pii: nbt.4066. doi: 10.1038/nbt.4066. PubMed

Associated Plasmids

SNX18 regulates ATG9A trafficking from recycling endosomes by recruiting Dynamin-2. Soreng K, Munson MJ, Lamb CA, Bjorndal GT, Pankiv S, Carlsson SR, Tooze SA, Simonsen A. EMBO Rep. 2018 Apr;19(4). pii: embr.201744837. doi: 10.15252/embr.201744837. Epub 2018 Feb 7. PubMed
Lineage specific transcription factors and epigenetic regulators mediate TGFbeta-dependent enhancer activation. Fueyo R, Iacobucci S, Pappa S, Estaras C, Lois S, Vicioso-Mantis M, Navarro C, Cruz-Molina S, Reyes JC, Rada-Iglesias A, de la Cruz X, Martinez-Balbas MA. Nucleic Acids Res. 2018 Apr 20;46(7):3351-3365. doi: 10.1093/nar/gky093. PubMed
Heritable, Allele-Specific Chromosomal Looping between Tandem Promoters Specifies Promoter Usage of SHC1. Li X, Lin Z, Wang H, Zhao D, Xu X, Wei Y, Li X, Li X, Xiang Y, Terada LS, Liu Z. Mol Cell Biol. 2018 Apr 16;38(9). pii: MCB.00658-17. doi: 10.1128/MCB.00658-17. Print 2018 May 1. PubMed
C8ORF37 Is Required for Photoreceptor Outer Segment Disc Morphogenesis by Maintaining Outer Segment Membrane Protein Homeostasis. Sharif AS, Yu D, Loertscher S, Austin R, Nguyen K, Mathur PD, Clark AM, Zou J, Lobanova ES, Arshavsky VY, Yang J. J Neurosci. 2018 Mar 28;38(13):3160-3176. doi: 10.1523/JNEUROSCI.2964-17.2018. Epub 2018 Feb 13. PubMed
TGFbeta drives immune evasion in genetically reconstituted colon cancer metastasis. Tauriello DVF, Palomo-Ponce S, Stork D, Berenguer-Llergo A, Badia-Ramentol J, Iglesias M, Sevillano M, Ibiza S, Canellas A, Hernando-Momblona X, Byrom D, Matarin JA, Calon A, Rivas EI, Nebreda AR, Riera A, Attolini CS, Batlle E. Nature. 2018 Feb 22;554(7693):538-543. doi: 10.1038/nature25492. Epub 2018 Feb 14. PubMed
DNA mismatch repair and oligonucleotide end-protection promote base-pair substitution distal from a CRISPR/Cas9-induced DNA break. Harmsen T, Klaasen S, van de Vrugt H, Te Riele H. Nucleic Acids Res. 2018 Apr 6;46(6):2945-2955. doi: 10.1093/nar/gky076. PubMed
Pold3 is required for genomic stability and telomere integrity in embryonic stem cells and meiosis. Zhou Z, Wang L, Ge F, Gong P, Wang H, Wang F, Chen L, Liu L. Nucleic Acids Res. 2018 Apr 20;46(7):3468-3486. doi: 10.1093/nar/gky098. PubMed
HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130. Goder A, Emmerich C, Nikolova T, Kiweler N, Schreiber M, Kuhl T, Imhof D, Christmann M, Heinzel T, Schneider G, Kramer OH. Nat Commun. 2018 Feb 22;9(1):764. doi: 10.1038/s41467-018-03096-0. PubMed
BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone. Ye F, Nager AR, Nachury MV. J Cell Biol. 2018 May 7;217(5):1847-1868. doi: 10.1083/jcb.201709041. Epub 2018 Feb 26. PubMed
RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells. Guallar D, Bi X, Pardavila JA, Huang X, Saenz C, Shi X, Zhou H, Faiola F, Ding J, Haruehanroengra P, Yang F, Li D, Sanchez-Priego C, Saunders A, Pan F, Valdes VJ, Kelley K, Blanco MG, Chen L, Wang H, Sheng J, Xu M, Fidalgo M, Shen X, Wang J. Nat Genet. 2018 Mar;50(3):443-451. doi: 10.1038/s41588-018-0060-9. Epub 2018 Feb 26. PubMed
Conserved roles of C. elegans and human MANFs in sulfatide binding and cytoprotection. Bai M, Vozdek R, Hnizda A, Jiang C, Wang B, Kuchar L, Li T, Zhang Y, Wood C, Feng L, Dang Y, Ma DK. Nat Commun. 2018 Mar 1;9(1):897. doi: 10.1038/s41467-018-03355-0. PubMed
Development of a multiple-gene-loading method by combining multi-integration system-equipped mouse artificial chromosome vector and CRISPR-Cas9. Honma K, Abe S, Endo T, Uno N, Oshimura M, Ohbayashi T, Kazuki Y. PLoS One. 2018 Mar 5;13(3):e0193642. doi: 10.1371/journal.pone.0193642. eCollection 2018. PubMed
Microhomology-assisted scarless genome editing in human iPSCs. Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K. Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y. PubMed

Associated Plasmids

MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB. Hsu CL, Lee EX, Gordon KL, Paz EA, Shen WC, Ohnishi K, Meisenhelder J, Hunter T, La Spada AR. Nat Commun. 2018 Mar 5;9(1):942. doi: 10.1038/s41467-018-03340-7. PubMed
Decapping protein EDC4 regulates DNA repair and phenocopies BRCA1. Hernandez G, Ramirez MJ, Minguillon J, Quiles P, Ruiz de Garibay G, Aza-Carmona M, Bogliolo M, Pujol R, Prados-Carvajal R, Fernandez J, Garcia N, Lopez A, Gutierrez-Enriquez S, Diez O, Benitez J, Salinas M, Teule A, Brunet J, Radice P, Peterlongo P, Schindler D, Huertas P, Puente XS, Lazaro C, Pujana MA, Surralles J. Nat Commun. 2018 Mar 6;9(1):967. doi: 10.1038/s41467-018-03433-3. PubMed
CRISPR-Cas9-Based Genome Editing of Human Induced Pluripotent Stem Cells. Giacalone JC, Sharma TP, Burnight ER, Fingert JF, Mullins RF, Stone EM, Tucker BA. Curr Protoc Stem Cell Biol. 2018 Feb 28;44:5B.7.1-5B.7.22. doi: 10.1002/cpsc.46. PubMed

Associated Plasmids

Epigenetic reprogramming enables the transition from primordial germ cell to gonocyte. Hill PWS, Leitch HG, Requena CE, Sun Z, Amouroux R, Roman-Trufero M, Borkowska M, Terragni J, Vaisvila R, Linnett S, Bagci H, Dharmalingham G, Haberle V, Lenhard B, Zheng Y, Pradhan S, Hajkova P. Nature. 2018 Mar 15;555(7696):392-396. doi: 10.1038/nature25964. Epub 2018 Mar 7. PubMed
Screening Regulatory Element Function with CRISPR/Cas9-based Epigenome Editing. Klann TS, Crawford GE, Reddy TE, Gersbach CA. Methods Mol Biol. 2018;1767:447-480. doi: 10.1007/978-1-4939-7774-1_25. PubMed
Easy quantification of template-directed CRISPR/Cas9 editing. Brinkman EK, Kousholt AN, Harmsen T, Leemans C, Chen T, Jonkers J, van Steensel B. Nucleic Acids Res. 2018 Jun 1;46(10):e58. doi: 10.1093/nar/gky164. PubMed
Intravesicular Acidification Regulates Lipopolysaccharide Inflammation and Tolerance through TLR4 Trafficking. Murase M, Kawasaki T, Hakozaki R, Sueyoshi T, Putri DDP, Kitai Y, Sato S, Ikawa M, Kawai T. J Immunol. 2018 Apr 15;200(8):2798-2808. doi: 10.4049/jimmunol.1701390. Epub 2018 Mar 14. PubMed
Pinpointing recurrent proviral integration sites in new models for latent HIV-1 infection. Lange UC, Bialek JK, Walther T, Hauber J. Virus Res. 2018 Apr 2;249:69-75. doi: 10.1016/j.virusres.2018.03.007. Epub 2018 Mar 14. PubMed
Synapse maintenance and restoration in the retina by NGL2. Soto F, Zhao L, Kerschensteiner D. Elife. 2018 Mar 19;7. pii: 30388. doi: 10.7554/eLife.30388. PubMed
Adjuvant Treatment for POLE Proofreading Domain-Mutant Cancers: Sensitivity to Radiotherapy, Chemotherapy, and Nucleoside Analogues. Van Gool IC, Rayner E, Osse EM, Nout RA, Creutzberg CL, Tomlinson IPM, Church DN, Smit VTHBM, de Wind N, Bosse T, Drost M. Clin Cancer Res. 2018 Jul 1;24(13):3197-3203. doi: 10.1158/1078-0432.CCR-18-0266. Epub 2018 Mar 20. PubMed
Neuropathic pain in a Fabry disease rat model. Miller JJ, Aoki K, Moehring F, Murphy CA, O'Hara CL, Tiemeyer M, Stucky CL, Dahms NM. JCI Insight. 2018 Mar 22;3(6). pii: 99171. doi: 10.1172/jci.insight.99171. PubMed
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy. Yao X, Wang X, Liu J, Shi L, Huang P, Yang H. J Vis Exp. 2018 Mar 12;(133). doi: 10.3791/56844. PubMed
Rescue of an aggressive female sexual courtship in mice by CRISPR/Cas9 secondary mutation in vivo. Zakany J, Duboule D. BMC Res Notes. 2018 Mar 27;11(1):193. doi: 10.1186/s13104-018-3307-8. PubMed
Paired D10A Cas9 nickases are sometimes more efficient than individual nucleases for gene disruption. Gopalappa R, Suresh B, Ramakrishna S, Kim HH. Nucleic Acids Res. 2018 Jul 6;46(12):e71. doi: 10.1093/nar/gky222. PubMed
An E2F7-dependent transcriptional program modulates DNA damage repair and genomic stability. Mitxelena J, Apraiz A, Vallejo-Rodriguez J, Garcia-Santisteban I, Fullaondo A, Alvarez-Fernandez M, Malumbres M, Zubiaga AM. Nucleic Acids Res. 2018 May 18;46(9):4546-4559. doi: 10.1093/nar/gky218. PubMed
MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation. Zhou P, Ding X, Wan X, Liu L, Yuan X, Zhang W, Hui X, Meng G, Xiao H, Li B, Zhong J, Hou F, Deng L, Zhang Y. Nat Commun. 2018 Mar 28;9(1):1243. doi: 10.1038/s41467-018-03563-8. PubMed
In Vivo Genome Editing Partially Restores Alpha1-Antitrypsin in a Murine Model of AAT Deficiency. Song CQ, Wang D, Jiang T, O'Connor K, Tang Q, Cai L, Li X, Weng Z, Yin H, Gao G, Mueller C, Flotte TR, Xue W. Hum Gene Ther. 2018 Aug;29(8):853-860. doi: 10.1089/hum.2017.225. Epub 2018 May 14. PubMed
A Transcriptional Circuit Filters Oscillating Circadian Hormonal Inputs to Regulate Fat Cell Differentiation. Bahrami-Nejad Z, Zhao ML, Tholen S, Hunerdosse D, Tkach KE, van Schie S, Chung M, Teruel MN. Cell Metab. 2018 Apr 3;27(4):854-868.e8. doi: 10.1016/j.cmet.2018.03.012. PubMed
A multi-landing pad DNA integration platform for mammalian cell engineering. Gaidukov L, Wroblewska L, Teague B, Nelson T, Zhang X, Liu Y, Jagtap K, Mamo S, Tseng WA, Lowe A, Das J, Bandara K, Baijuraj S, Summers NM, Lu TK, Zhang L, Weiss R. Nucleic Acids Res. 2018 May 4;46(8):4072-4086. doi: 10.1093/nar/gky216. PubMed
Increasing antigen presentation on HSV-1-infected cells increases lesion size but does not alter neural infection or latency. Russell TA, Velusamy T, Tseng YY, Tscharke DC. J Gen Virol. 2018 May;99(5):682-692. doi: 10.1099/jgv.0.001059. Epub 2018 Apr 5. PubMed
Carbonic anhydrase IX is a critical determinant of pulmonary microvascular endothelial cell pH regulation and angiogenesis during acidosis. Lee JY, Alexeyev M, Kozhukhar N, Pastukh V, White R, Stevens T. Am J Physiol Lung Cell Mol Physiol. 2018 Jul 1;315(1):L41-L51. doi: 10.1152/ajplung.00446.2017. Epub 2018 Apr 5. PubMed
GDF15 promotes the proliferation of cervical cancer cells by phosphorylating AKT1 and Erk1/2 through the receptor ErbB2. Li S, Ma YM, Zheng PS, Zhang P. J Exp Clin Cancer Res. 2018 Apr 10;37(1):80. doi: 10.1186/s13046-018-0744-0. PubMed
Repeat-mediated deletions can be induced by a chromosomal break far from a repeat, but multiple pathways suppress such rearrangements. Mendez-Dorantes C, Bhargava R, Stark JM. Genes Dev. 2018 Apr 1;32(7-8):524-536. doi: 10.1101/gad.311084.117. Epub 2018 Apr 10. PubMed
Transcriptional Dysregulation of MYC Reveals Common Enhancer-Docking Mechanism. Schuijers J, Manteiga JC, Weintraub AS, Day DS, Zamudio AV, Hnisz D, Lee TI, Young RA. Cell Rep. 2018 Apr 10;23(2):349-360. doi: 10.1016/j.celrep.2018.03.056. PubMed
CRISPR/Cas9 Inhibits Multiple Steps of HIV-1 Infection. Yin L, Hu S, Mei S, Sun H, Xu F, Li J, Zhu W, Liu X, Zhao F, Zhang D, Cen S, Liang C, Guo F. Hum Gene Ther. 2018 Nov;29(11):1264-1276. doi: 10.1089/hum.2018.018. Epub 2018 May 9. PubMed
Suppressing Ku70/Ku80 expression elevates homology-directed repair efficiency in primary fibroblasts. Li G, Liu D, Zhang X, Quan R, Zhong C, Mo J, Huang Y, Wang H, Ruan X, Xu Z, Zheng E, Gu T, Hong L, Li Z, Wu Z, Yang H. Int J Biochem Cell Biol. 2018 Jun;99:154-160. doi: 10.1016/j.biocel.2018.04.011. Epub 2018 Apr 12. PubMed
Crystal structure of intraflagellar transport protein 80 reveals a homo-dimer required for ciliogenesis. Taschner M, Lorentzen A, Mourao A, Collins T, Freke GM, Moulding D, Basquin J, Jenkins D, Lorentzen E. Elife. 2018 Apr 16;7. pii: 33067. doi: 10.7554/eLife.33067. PubMed
Conserved and divergent functions of Pax6 underlie species-specific neurogenic patterns in the developing amniote brain. Yamashita W, Takahashi M, Kikkawa T, Gotoh H, Osumi N, Ono K, Nomura T. Development. 2018 Apr 16;145(8). pii: 145/8/dev159764. doi: 10.1242/dev.159764. PubMed
Comparative Analysis of UL16 Mutants Derived from Multiple Strains of Herpes Simplex Virus 2 (HSV-2) and HSV-1 Reveals Species-Specific Requirements for the UL16 Protein. Gao J, Yan X, Banfield BW. J Virol. 2018 Jun 13;92(13). pii: JVI.00629-18. doi: 10.1128/JVI.00629-18. Print 2018 Jul 1. PubMed
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair. Kato-Inui T, Takahashi G, Hsu S, Miyaoka Y. Nucleic Acids Res. 2018 May 18;46(9):4677-4688. doi: 10.1093/nar/gky264. PubMed
Physiological function of phospholipase D2 in anti-tumor immunity: regulation of CD8(+) T lymphocyte proliferation. Ngo Thai Bich V, Hongu T, Miura Y, Katagiri N, Ohbayashi N, Yamashita-Kanemaru Y, Shibuya A, Funakoshi Y, Kanaho Y. Sci Rep. 2018 Apr 19;8(1):6283. doi: 10.1038/s41598-018-24512-x. PubMed
KREMEN1 Is a Host Entry Receptor for a Major Group of Enteroviruses. Staring J, van den Hengel LG, Raaben M, Blomen VA, Carette JE, Brummelkamp TR. Cell Host Microbe. 2018 May 9;23(5):636-643.e5. doi: 10.1016/j.chom.2018.03.019. Epub 2018 Apr 19. PubMed
Loss of tafazzin results in decreased myoblast differentiation in C2C12 cells: A myoblast model of Barth syndrome and cardiolipin deficiency. Lou W, Reynolds CA, Li Y, Liu J, Huttemann M, Schlame M, Stevenson D, Strathdee D, Greenberg ML. Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Aug;1863(8):857-865. doi: 10.1016/j.bbalip.2018.04.015. Epub 2018 Apr 22. PubMed
Role of Complement in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy. Xu J, Zhang L, Xie M, Li Y, Huang P, Saunders TL, Fox DA, Rosenquist R, Lin F. J Immunol. 2018 Jun 15;200(12):4094-4101. doi: 10.4049/jimmunol.1701716. Epub 2018 Apr 25. PubMed
Mental Illnesses-Associated Fxr1 and Its Negative Regulator Gsk3beta Are Modulators of Anxiety and Glutamatergic Neurotransmission. Khlghatyan J, Evstratova A, Chamberland S, Marakhovskaia A, Bahremand A, Toth K, Beaulieu JM. Front Mol Neurosci. 2018 Apr 12;11:119. doi: 10.3389/fnmol.2018.00119. eCollection 2018. PubMed

Associated Plasmids

Complex formation between the vasopressin 1b receptor, beta-arrestin-2, and the mu-opioid receptor underlies morphine tolerance. Koshimizu TA, Honda K, Nagaoka-Uozumi S, Ichimura A, Kimura I, Nakaya M, Sakai N, Shibata K, Ushijima K, Fujimura A, Hirasawa A, Kurose H, Tsujimoto G, Tanoue A, Takano Y. Nat Neurosci. 2018 Jun;21(6):820-833. doi: 10.1038/s41593-018-0144-y. Epub 2018 Apr 30. PubMed
Generation of App knock-in mice reveals deletion mutations protective against Alzheimer's disease-like pathology. Nagata K, Takahashi M, Matsuba Y, Okuyama-Uchimura F, Sato K, Hashimoto S, Saito T, Saido TC. Nat Commun. 2018 May 4;9(1):1800. doi: 10.1038/s41467-018-04238-0. PubMed
Tissue Distribution of Kir7.1 Inwardly Rectifying K(+) Channel Probed in a Knock-in Mouse Expressing a Haemagglutinin-Tagged Protein. Cornejo I, Villanueva S, Burgos J, Lopez-Cayuqueo KI, Chambrey R, Julio-Kalajzic F, Buelvas N, Niemeyer MI, Figueiras-Fierro D, Brown PD, Sepulveda FV, Cid LP. Front Physiol. 2018 Apr 23;9:428. doi: 10.3389/fphys.2018.00428. eCollection 2018. PubMed
CD155 loss enhances tumor suppression via combined host and tumor-intrinsic mechanisms. Li XY, Das I, Lepletier A, Addala V, Bald T, Stannard K, Barkauskas D, Liu J, Aguilera AR, Takeda K, Braun M, Nakamura K, Jacquelin S, Lane SW, Teng MW, Dougall WC, Smyth MJ. J Clin Invest. 2018 Jun 1;128(6):2613-2625. doi: 10.1172/JCI98769. Epub 2018 May 14. PubMed
CO2-sensitive tRNA modification associated with human mitochondrial disease. Lin H, Miyauchi K, Harada T, Okita R, Takeshita E, Komaki H, Fujioka K, Yagasaki H, Goto YI, Yanaka K, Nakagawa S, Sakaguchi Y, Suzuki T. Nat Commun. 2018 May 14;9(1):1875. doi: 10.1038/s41467-018-04250-4. PubMed
CRISPR/Cas9 Mutagenesis of UL21 in Multiple Strains of Herpes Simplex Virus Reveals Differential Requirements for pUL21 in Viral Replication. Finnen RL, Banfield BW. Viruses. 2018 May 15;10(5). pii: v10050258. doi: 10.3390/v10050258. PubMed
A Critical Neurodevelopmental Role for L-Type Voltage-Gated Calcium Channels in Neurite Extension and Radial Migration. Kamijo S, Ishii Y, Horigane SI, Suzuki K, Ohkura M, Nakai J, Fujii H, Takemoto-Kimura S, Bito H. J Neurosci. 2018 Jun 13;38(24):5551-5566. doi: 10.1523/JNEUROSCI.2357-17.2018. Epub 2018 May 17. PubMed
Plasmodium parasite exploits host aquaporin-3 during liver stage malaria infection. Posfai D, Sylvester K, Reddy A, Ganley JG, Wirth J, Cullen QE, Dave T, Kato N, Dave SS, Derbyshire ER. PLoS Pathog. 2018 May 18;14(5):e1007057. doi: 10.1371/journal.ppat.1007057. eCollection 2018 May. PubMed
Derivation and characterization of a UCP1 reporter human ES cell line. Mukherjee S, Zhang T, Lacko LA, Tan L, Xiang JZ, Butler JM, Chen S. Stem Cell Res. 2018 Jul;30:12-21. doi: 10.1016/j.scr.2018.04.007. Epub 2018 Apr 22. PubMed
In vivo gene correction with targeted sequence substitution through microhomology-mediated end joining. Shin JH, Jung S, Ramakrishna S, Kim HH, Lee J. Biochem Biophys Res Commun. 2018 Jul 7;502(1):116-122. doi: 10.1016/j.bbrc.2018.05.130. Epub 2018 May 24. PubMed
Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Tsuchiya M, Hara Y, Okuda M, Itoh K, Nishioka R, Shiomi A, Nagao K, Mori M, Mori Y, Ikenouchi J, Suzuki R, Tanaka M, Ohwada T, Aoki J, Kanagawa M, Toda T, Nagata Y, Matsuda R, Takayama Y, Tominaga M, Umeda M. Nat Commun. 2018 May 24;9(1):2049. doi: 10.1038/s41467-018-04436-w. PubMed
Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells. Lambrus BG, Moyer TC, Holland AJ. Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. PubMed
Kinetics and Fidelity of the Repair of Cas9-Induced Double-Strand DNA Breaks. Brinkman EK, Chen T, de Haas M, Holland HA, Akhtar W, van Steensel B. Mol Cell. 2018 Jun 7;70(5):801-813.e6. doi: 10.1016/j.molcel.2018.04.016. Epub 2018 May 24. PubMed
Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T. Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559. PubMed

Associated Plasmids

Combinatorial knockout of RARalpha, RARbeta, and RARgamma completely abrogates transcriptional responses to retinoic acid in murine embryonic stem cells. Laursen KB, Gudas LJ. J Biol Chem. 2018 Jul 27;293(30):11891-11900. doi: 10.1074/jbc.RA118.001951. Epub 2018 May 30. PubMed
Amino Acid Variation at VP1-145 of Enterovirus 71 Determines Attachment Receptor Usage and Neurovirulence in Human Scavenger Receptor B2 Transgenic Mice. Kobayashi K, Sudaka Y, Takashino A, Imura A, Fujii K, Koike S. J Virol. 2018 Jul 17;92(15). pii: JVI.00681-18. doi: 10.1128/JVI.00681-18. Print 2018 Aug 1. PubMed
CRISPR/Cas9-mediated gene knockout for DNA methyltransferase Dnmt3a in CHO cells displays enhanced transgenic expression and long-term stability. Jia YL, Guo X, Lu JT, Wang XY, Qiu LL, Wang TY. J Cell Mol Med. 2018 Sep;22(9):4106-4116. doi: 10.1111/jcmm.13687. Epub 2018 May 30. PubMed
Efficient genome editing by FACS enrichment of paired D10A Cas9 nickases coupled with fluorescent proteins. Gopalappa R, Song M, Chandrasekaran AP, Das S, Haq S, Koh HC, Ramakrishna S. Arch Pharm Res. 2018 Sep;41(9):911-920. doi: 10.1007/s12272-018-1042-2. Epub 2018 May 31. PubMed
CRISPR/Cas9-Mediated Genome Editing for Huntington's Disease. Vachey G, Deglon N. Methods Mol Biol. 2018;1780:463-481. doi: 10.1007/978-1-4939-7825-0_21. PubMed
Insights into the mechanisms underlying the inactivation of HIV-1 proviruses by CRISPR/Cas. Mefferd AL, Bogerd HP, Irwan ID, Cullen BR. Virology. 2018 Jul;520:116-126. doi: 10.1016/j.virol.2018.05.016. Epub 2018 May 29. PubMed
Generation of Hutat2:Fc Knockin Primary Human Monocytes Using CRISPR/Cas9. Wang B, Zuo J, Kang W, Wei Q, Li J, Wang C, Liu Z, Lu Y, Zhuang Y, Dang B, Liu Q, Kang W, Sun Y. Mol Ther Nucleic Acids. 2018 Jun 1;11:130-141. doi: 10.1016/j.omtn.2018.01.012. Epub 2018 Feb 7. PubMed
Depleting Trim28 in adult mice is well tolerated and reduces levels of alpha-synuclein and tau. Rousseaux MW, Revelli JP, Vazquez-Velez GE, Kim JY, Craigen E, Gonzales K, Beckinghausen J, Zoghbi HY. Elife. 2018 Jun 4;7. pii: 36768. doi: 10.7554/eLife.36768. PubMed
Global untargeted serum metabolomic analyses nominate metabolic pathways responsive to loss of expression of the orphan metallo beta-lactamase, MBLAC1. Gibson CL, Codreanu SG, Schrimpe-Rutledge AC, Retzlaff CL, Wright J, Mortlock DP, Sherrod SD, McLean JA, Blakely RD. Mol Omics. 2018 Jun 12;14(3):142-155. doi: 10.1039/c7mo00022g. PubMed
Biallelic UFM1 and UFC1 mutations expand the essential role of ufmylation in brain development. Nahorski MS, Maddirevula S, Ishimura R, Alsahli S, Brady AF, Begemann A, Mizushima T, Guzman-Vega FJ, Obata M, Ichimura Y, Alsaif HS, Anazi S, Ibrahim N, Abdulwahab F, Hashem M, Monies D, Abouelhoda M, Meyer BF, Alfadhel M, Eyaid W, Zweier M, Steindl K, Rauch A, Arold ST, Woods CG, Komatsu M, Alkuraya FS. Brain. 2018 Jul 1;141(7):1934-1945. doi: 10.1093/brain/awy135. PubMed
MICMIC: identification of DNA methylation of distal regulatory regions with causal effects on tumorigenesis. Tong Y, Sun J, Wong CF, Kang Q, Ru B, Wong CN, Chan AS, Leung SY, Zhang J. Genome Biol. 2018 Jun 5;19(1):73. doi: 10.1186/s13059-018-1442-0. PubMed
Diminished nuclear RNA decay upon Salmonella infection upregulates antibacterial noncoding RNAs. Imamura K, Takaya A, Ishida YI, Fukuoka Y, Taya T, Nakaki R, Kakeda M, Imamachi N, Sato A, Yamada T, Onoguchi-Mizutani R, Akizuki G, Tanu T, Tao K, Miyao S, Suzuki Y, Nagahama M, Yamamoto T, Jensen TH, Akimitsu N. EMBO J. 2018 Jul 2;37(13). pii: embj.201797723. doi: 10.15252/embj.201797723. Epub 2018 Jun 7. PubMed
Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Gu B, Posfai E, Rossant J. Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. PubMed

Associated Plasmids

iTAP, a novel iRhom interactor, controls TNF secretion by policing the stability of iRhom/TACE. Oikonomidi I, Burbridge E, Cavadas M, Sullivan G, Collis B, Naegele H, Clancy D, Brezinova J, Hu T, Bileck A, Gerner C, Bolado A, von Kriegsheim A, Martin SJ, Steinberg F, Strisovsky K, Adrain C. Elife. 2018 Jun 13;7. pii: 35032. doi: 10.7554/eLife.35032. PubMed
Disruption of a -35 kb Enhancer Impairs CTCF Binding and MLH1 Expression in Colorectal Cells. Liu Q, Thoms JAI, Nunez AC, Huang Y, Knezevic K, Packham D, Poulos RC, Williams R, Beck D, Hawkins NJ, Ward RL, Wong JWH, Hesson LB, Sloane MA, Pimanda JE. Clin Cancer Res. 2018 Sep 15;24(18):4602-4611. doi: 10.1158/1078-0432.CCR-17-3678. Epub 2018 Jun 13. PubMed
A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins alpha3beta1, alphaVbeta3, and alphaVbeta5. TerBush AA, Hafkamp F, Lee HJ, Coscoy L. J Virol. 2018 Aug 16;92(17). pii: JVI.00803-18. doi: 10.1128/JVI.00803-18. Print 2018 Sep 1. PubMed
Efficient Knock-in of a Point Mutation in Porcine Fibroblasts Using the CRISPR/Cas9-GMNN Fusion Gene. Gerlach M, Kraft T, Brenner B, Petersen B, Niemann H, Montag J. Genes (Basel). 2018 Jun 13;9(6). pii: genes9060296. doi: 10.3390/genes9060296. PubMed
The chemerin knockout rat reveals chemerin dependence in female, but not male, experimental hypertension. Watts SW, Darios ES, Mullick AE, Garver H, Saunders TL, Hughes ED, Filipiak WE, Zeidler MG, McMullen N, Sinal CJ, Kumar RK, Ferland DJ, Fink GD. FASEB J. 2018 Jun 15:fj201800479. doi: 10.1096/fj.201800479. PubMed
Foot-and-mouth disease virus nonstructural protein 2B interacts with cyclophilin A, modulating virus replication. Liu H, Xue Q, Cao W, Yang F, Ma L, Liu W, Zhang K, Liu X, Zhu Z, Zheng H. FASEB J. 2018 Jun 15:fj201701351. doi: 10.1096/fj.201701351. PubMed
Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency. Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR. Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14. PubMed

Associated Plasmids

Exploiting CRISPR-Cas9 technology to investigate individual histone modifications. Vasquez JJ, Wedel C, Cosentino RO, Siegel TN. Nucleic Acids Res. 2018 Oct 12;46(18):e106. doi: 10.1093/nar/gky517. PubMed
ZMYND10 functions in a chaperone relay during axonemal dynein assembly. Mali GR, Yeyati PL, Mizuno S, Dodd DO, Tennant PA, Keighren MA, Zur Lage P, Shoemark A, Garcia-Munoz A, Shimada A, Takeda H, Edlich F, Takahashi S, von Kreigsheim A, Jarman AP, Mill P. Elife. 2018 Jun 19;7. pii: 34389. doi: 10.7554/eLife.34389. PubMed
Investigation of the role of GBF1 in the replication of positive-sense single-stranded RNA viruses. Ferlin J, Farhat R, Belouzard S, Cocquerel L, Bertin A, Hober D, Dubuisson J, Rouille Y. J Gen Virol. 2018 Aug;99(8):1086-1096. doi: 10.1099/jgv.0.001099. Epub 2018 Jun 20. PubMed
Novel lymphoid enhancer-binding factor 1-cytoglobin axis promotes extravasation of osteosarcoma cells into the lungs. Pongsuchart M, Kuchimaru T, Yonezawa S, Tran DTP, Kha NT, Hoang NTH, Kadonosono T, Kizaka-Kondoh S. Cancer Sci. 2018 Sep;109(9):2746-2756. doi: 10.1111/cas.13702. Epub 2018 Jul 24. PubMed
Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus. Kitamura K, Que L, Shimadu M, Koura M, Ishihara Y, Wakae K, Nakamura T, Watashi K, Wakita T, Muramatsu M. PLoS Pathog. 2018 Jun 21;14(6):e1007124. doi: 10.1371/journal.ppat.1007124. eCollection 2018 Jun. PubMed
CRISPR/Cas and recombinase-based human-to-pig orthotopic gene exchange for xenotransplantation. Nunes Dos Santos RM, Carneiro D'Albuquerque LA, Reyes LM, Estrada JL, Wang ZY, Tector M, Tector AJ. J Surg Res. 2018 Sep;229:28-40. doi: 10.1016/j.jss.2018.03.051. Epub 2018 Apr 16. PubMed
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer. Feng W, Herbst L, Lichter P, Pfister SM, Liu HK, Kawauchi D. J Vis Exp. 2018 Jun 9;(136). doi: 10.3791/57311. PubMed
Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg(-/-) mice. Du Y, Wang T, Xu J, Zhao C, Li H, Fu Y, Xu Y, Xie L, Zhao J, Yang W, Yin M, Wen J, Deng H. Protein Cell. 2019 Jan;10(1):31-42. doi: 10.1007/s13238-018-0558-z. Epub 2018 Jun 13. PubMed
Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation. Li H, Zhao C, Xu J, Xu Y, Cheng C, Liu Y, Wang T, Du Y, Xie L, Zhao J, Han Y, Wang X, Bai Y, Deng H. Protein Cell. 2019 Jan;10(1):20-30. doi: 10.1007/s13238-018-0556-1. Epub 2018 Jun 13. PubMed
Mitoregulin: A lncRNA-Encoded Microprotein that Supports Mitochondrial Supercomplexes and Respiratory Efficiency. Stein CS, Jadiya P, Zhang X, McLendon JM, Abouassaly GM, Witmer NH, Anderson EJ, Elrod JW, Boudreau RL. Cell Rep. 2018 Jun 26;23(13):3710-3720.e8. doi: 10.1016/j.celrep.2018.06.002. PubMed
C-NHEJ without indels is robust and requires synergistic function of distinct XLF domains. Bhargava R, Sandhu M, Muk S, Lee G, Vaidehi N, Stark JM. Nat Commun. 2018 Jun 27;9(1):2484. doi: 10.1038/s41467-018-04867-5. PubMed

Associated Plasmids

The polycomb group protein Yaf2 regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner. Zhao W, Liu M, Ji H, Zhu Y, Wang C, Huang Y, Ma X, Xing G, Xia Y, Jiang Q, Qin J. J Biol Chem. 2018 Aug 17;293(33):12793-12804. doi: 10.1074/jbc.RA118.003299. Epub 2018 Jun 29. PubMed
Generation of the Adenovirus Vector-Mediated CRISPR/Cpf1 System and the Application for Primary Human Hepatocytes Prepared from Humanized Mice with Chimeric Liver. Tsukamoto T, Sakai E, Iizuka S, Taracena-Gandara M, Sakurai F, Mizuguchi H. Biol Pharm Bull. 2018;41(7):1089-1095. doi: 10.1248/bpb.b18-00222. PubMed
Near infrared fluorescent peptide nanoparticles for enhancing esophageal cancer therapeutic efficacy. Fan Z, Chang Y, Cui C, Sun L, Wang DH, Pan Z, Zhang M. Nat Commun. 2018 Jul 4;9(1):2605. doi: 10.1038/s41467-018-04763-y. PubMed
Prolactin signaling drives tumorigenesis in human high grade serous ovarian cancer cells and in a spontaneous fallopian tube derived model. Karthikeyan S, Russo A, Dean M, Lantvit DD, Endsley M, Burdette JE. Cancer Lett. 2018 Oct 1;433:221-231. doi: 10.1016/j.canlet.2018.07.003. Epub 2018 Jul 5. PubMed
The N Termini of TAR DNA-Binding Protein 43 (TDP43) C-Terminal Fragments Influence Degradation, Aggregation Propensity, and Morphology. Kasu YAT, Alemu S, Lamari A, Loew N, Brower CS. Mol Cell Biol. 2018 Sep 14;38(19). pii: MCB.00243-18. doi: 10.1128/MCB.00243-18. Print 2018 Oct 1. PubMed
Oncogenic addiction to high 26S proteasome level. Tsvetkov P, Adler J, Myers N, Biran A, Reuven N, Shaul Y. Cell Death Dis. 2018 Jul 10;9(7):773. doi: 10.1038/s41419-018-0806-4. PubMed
Discovery of a drug candidate for GLIS3-associated diabetes. Amin S, Cook B, Zhou T, Ghazizadeh Z, Lis R, Zhang T, Khalaj M, Crespo M, Perera M, Xiang JZ, Zhu Z, Tomishima M, Liu C, Naji A, Evans T, Huangfu D, Chen S. Nat Commun. 2018 Jul 11;9(1):2681. doi: 10.1038/s41467-018-04918-x. PubMed
Optimized Plasmid Construction Strategy for Cas9. Xu J, Li W, Hossen MM, Jia Y, Li L, Huang Z. Cell Physiol Biochem. 2018;48(1):131-137. doi: 10.1159/000491669. Epub 2018 Jul 12. PubMed
gamma-Secretase promotes membrane insertion of the human papillomavirus L2 capsid protein during virus infection. Inoue T, Zhang P, Zhang W, Goodner-Bingham K, Dupzyk A, DiMaio D, Tsai B. J Cell Biol. 2018 Oct 1;217(10):3545-3559. doi: 10.1083/jcb.201804171. Epub 2018 Jul 13. PubMed
CRISPR/Cas9-mediated homologous recombination in tobacco. Hirohata A, Sato I, Kaino K, Iwata Y, Koizumi N, Mishiba KI. Plant Cell Rep. 2018 Jul 13. pii: 10.1007/s00299-018-2320-7. doi: 10.1007/s00299-018-2320-7. PubMed
A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality. Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA, Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT, Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, Waters J, Li L, Tasic B, Zeng H. Cell. 2018 Jul 12;174(2):465-480.e22. doi: 10.1016/j.cell.2018.06.035. PubMed

Associated Plasmids

Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2. Sevim H, Kocaefe YC, Onur MA, Uckan-Cetinkaya D, Gurpinar OA. Stem Cell Res Ther. 2018 Jul 18;9(1):198. doi: 10.1186/s13287-018-0941-y. PubMed
The shieldin complex mediates 53BP1-dependent DNA repair. Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY, Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, Lord CJ, Rottenberg S, Durocher D. Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. Epub 2018 Jul 18. PubMed

Associated Plasmids

HUWE1 controls the development of non-small cell lung cancer through down-regulation of p53. Yang D, Cheng D, Tu Q, Yang H, Sun B, Yan L, Dai H, Luo J, Mao B, Cao Y, Yu X, Jiang H, Zhao X. Theranostics. 2018 Jun 6;8(13):3517-3529. doi: 10.7150/thno.24401. eCollection 2018. PubMed
Translational Control through Differential Ribosome Pausing during Amino Acid Limitation in Mammalian Cells. Darnell AM, Subramaniam AR, O'Shea EK. Mol Cell. 2018 Jul 19;71(2):229-243.e11. doi: 10.1016/j.molcel.2018.06.041. PubMed
Exposure to galactic cosmic radiation compromises DNA repair and increases the potential for oncogenic chromosomal rearrangement in bronchial epithelial cells. Li Z, Jella KK, Jaafar L, Li S, Park S, Story MD, Wang H, Wang Y, Dynan WS. Sci Rep. 2018 Jul 23;8(1):11038. doi: 10.1038/s41598-018-29350-5. PubMed
Engineering Point Mutant and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease. Gertsenstein M, Nutter LMJ. Curr Protoc Mouse Biol. 2018 Mar;8(1):28-53. doi: 10.1002/cpmo.40. PubMed
53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ. Ghezraoui H, Oliveira C, Becker JR, Bilham K, Moralli D, Anzilotti C, Fischer R, Deobagkar-Lele M, Sanchiz-Calvo M, Fueyo-Marcos E, Bonham S, Kessler BM, Rottenberg S, Cornall RJ, Green CM, Chapman JR. Nature. 2018 Aug;560(7716):122-127. doi: 10.1038/s41586-018-0362-1. Epub 2018 Jul 25. PubMed
Chemogenetic Isolation Reveals Synaptic Contribution of delta GABAA Receptors in Mouse Dentate Granule Neurons. Sun MY, Shu HJ, Benz A, Bracamontes J, Akk G, Zorumski CF, Steinbach JH, Mennerick SJ. J Neurosci. 2018 Sep 19;38(38):8128-8145. doi: 10.1523/JNEUROSCI.0799-18.2018. Epub 2018 Aug 3. PubMed
Somatic Depdc5 deletion recapitulates electroclinical features of human focal cortical dysplasia type IIA. Hu S, Knowlton RC, Watson BO, Glanowska KM, Murphy GG, Parent JM, Wang Y. Ann Neurol. 2018 Jul;84(1):140-146. doi: 10.1002/ana.25272. Epub 2018 Aug 6. PubMed
Reconstruction of the cell entry pathway of an extinct virus. Robinson-McCarthy LR, McCarthy KR, Raaben M, Piccinotti S, Nieuwenhuis J, Stubbs SH, Bakkers MJG, Whelan SPJ. PLoS Pathog. 2018 Aug 6;14(8):e1007123. doi: 10.1371/journal.ppat.1007123. eCollection 2018 Aug. PubMed
Disruption of C1galt1 Gene Promotes Development and Metastasis of Pancreatic Adenocarcinomas in Mice. Chugh S, Barkeer S, Rachagani S, Nimmakayala RK, Perumal N, Pothuraju R, Atri P, Mahapatra S, Thapa I, Talmon GA, Smith LM, Yu X, Neelamegham S, Fu J, Xia L, Ponnusamy MP, Batra SK. Gastroenterology. 2018 Nov;155(5):1608-1624. doi: 10.1053/j.gastro.2018.08.007. Epub 2018 Aug 4. PubMed
A dual fluorescence reporter system for high throughput screening of effectors of Kiss1 gene expression. Li X, Wang S, Lu Y, Yin H, Xiao J, Li K, Ma L, Zhou Y. FEBS Open Bio. 2018 Jul 3;8(8):1352-1363. doi: 10.1002/2211-5463.12476. eCollection 2018 Aug. PubMed
Live cell imaging of X chromosome reactivation during somatic cell reprogramming. Tran THY, Fukuda A, Aizawa S, Bui PL, Hayashi Y, Nishimura K, Hisatake K. Biochem Biophys Rep. 2018 Jul 24;15:86-92. doi: 10.1016/j.bbrep.2018.07.007. eCollection 2018 Sep. PubMed
Temporal and Spatial Epigenome Editing Allows Precise Gene Regulation in Mammalian Cells. Kuscu C, Mammadov R, Czikora A, Unlu H, Tufan T, Fischer NL, Arslan S, Bekiranov S, Kanemaki M, Adli M. J Mol Biol. 2019 Jan 4;431(1):111-121. doi: 10.1016/j.jmb.2018.08.001. Epub 2018 Aug 9. PubMed
Endogenous HIF2A reporter systems for high-throughput functional screening. Zaini MN, Patel SA, Syafruddin SE, Rodrigues P, Vanharanta S. Sci Rep. 2018 Aug 13;8(1):12063. doi: 10.1038/s41598-018-30499-2. PubMed
Knock-In Mice with Myo3a Y137C Mutation Displayed Progressive Hearing Loss and Hair Cell Degeneration in the Inner Ear. Li P, Wen Z, Zhang G, Zhang A, Fu X, Gao J. Neural Plast. 2018 Jul 5;2018:4372913. doi: 10.1155/2018/4372913. eCollection 2018. PubMed
De Novo Mutations Activating Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome. Toki T, Yoshida K, Wang R, Nakamura S, Maekawa T, Goi K, Katoh MC, Mizuno S, Sugiyama F, Kanezaki R, Uechi T, Nakajima Y, Sato Y, Okuno Y, Sato-Otsubo A, Shiozawa Y, Kataoka K, Shiraishi Y, Sanada M, Chiba K, Tanaka H, Terui K, Sato T, Kamio T, Sakaguchi H, Ohga S, Kuramitsu M, Hamaguchi I, Ohara A, Kanno H, Miyano S, Kojima S, Ishiguro A, Sugita K, Kenmochi N, Takahashi S, Eto K, Ogawa S, Ito E. Am J Hum Genet. 2018 Sep 6;103(3):440-447. doi: 10.1016/j.ajhg.2018.07.020. Epub 2018 Aug 23. PubMed
Evaluation of Organic Anion Transporter 1A2-knock-in Mice as a Model of Human Blood-brain Barrier. Sano Y, Mizuno T, Mochizuki T, Uchida Y, Umetsu M, Terasaki T, Kusuhara H. Drug Metab Dispos. 2018 Nov;46(11):1767-1775. doi: 10.1124/dmd.118.081877. Epub 2018 Aug 28. PubMed
Tprn is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice. Men Y, Li X, Tu H, Zhang A, Fu X, Wang Z, Jin Y, Hou C, Zhang T, Zhang S, Zhou Y, Li B, Li J, Sun X, Wang H, Gao J. Front Med. 2018 Aug 30. pii: 10.1007/s11684-018-0638-8. doi: 10.1007/s11684-018-0638-8. PubMed
Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function. Laustsen A, Bak RO, Krapp C, Kjaer L, Egedahl JH, Petersen CC, Pillai S, Tang HQ, Uldbjerg N, Porteus M, Roan NR, Nyegaard M, Denton PW, Jakobsen MR. Nat Commun. 2018 Aug 30;9(1):3525. doi: 10.1038/s41467-018-05816-y. PubMed
Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing. Pankowicz FP, Barzi M, Kim KH, Legras X, Martins CS, Wooton-Kee CR, Lagor WR, Marini JC, Elsea SH, Bissig-Choisat B, Moore DD, Bissig KD. Gastroenterology. 2018 Dec;155(6):1967-1970.e6. doi: 10.1053/j.gastro.2018.08.037. Epub 2018 Aug 28. PubMed
4D cell biology: big data image analytics and lattice light-sheet imaging reveal dynamics of clathrin-mediated endocytosis in stem cell-derived intestinal organoids. Schoneberg J, Dambournet D, Liu TL, Forster R, Hockemeyer D, Betzig E, Drubin DG. Mol Biol Cell. 2018 Nov 26;29(24):2959-2968. doi: 10.1091/mbc.E18-06-0375. Epub 2018 Sep 6. PubMed
Whole exome sequencing of ENU-induced thrombosis modifier mutations in the mouse. Tomberg K, Westrick RJ, Kotnik EN, Cleuren AC, Siemieniak DR, Zhu G, Saunders TL, Ginsburg D. PLoS Genet. 2018 Sep 6;14(9):e1007658. doi: 10.1371/journal.pgen.1007658. eCollection 2018 Sep. PubMed
One-Vector System for Multiplexed CRISPR/Cas9 against Hepatitis B Virus cccDNA Utilizing High-Capacity Adenoviral Vectors. Schiwon M, Ehrke-Schulz E, Oswald A, Bergmann T, Michler T, Protzer U, Ehrhardt A. Mol Ther Nucleic Acids. 2018 Sep 7;12:242-253. doi: 10.1016/j.omtn.2018.05.006. Epub 2018 Jun 7. PubMed
VSV-G-Enveloped Vesicles for Traceless Delivery of CRISPR-Cas9. Montagna C, Petris G, Casini A, Maule G, Franceschini GM, Zanella I, Conti L, Arnoldi F, Burrone OR, Zentilin L, Zacchigna S, Giacca M, Cereseto A. Mol Ther Nucleic Acids. 2018 Sep 7;12:453-462. doi: 10.1016/j.omtn.2018.05.010. Epub 2018 Jul 11. PubMed
3-Aminobenzamide Prevents Concanavalin A-Induced Acute Hepatitis by an Anti-inflammatory and Anti-oxidative Mechanism. Wardi J, Ernst O, Lilja A, Aeed H, Katz S, Ben-Nachum I, Ben-Dror I, Katz D, Bernadsky O, Kandhikonda R, Avni Y, Fraser IDC, Weinstain R, Biro A, Zor T. Dig Dis Sci. 2018 Dec;63(12):3382-3397. doi: 10.1007/s10620-018-5267-1. Epub 2018 Sep 8. PubMed
Opposing Roles of FANCJ and HLTF Protect Forks and Restrain Replication during Stress. Peng M, Cong K, Panzarino NJ, Nayak S, Calvo J, Deng B, Zhu LJ, Morocz M, Hegedus L, Haracska L, Cantor SB. Cell Rep. 2018 Sep 18;24(12):3251-3261. doi: 10.1016/j.celrep.2018.08.065. PubMed
Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Fauster A, Rebsamen M, Willmann KL, Cesar-Razquin A, Girardi E, Bigenzahn JW, Schischlik F, Scorzoni S, Bruckner M, Konecka J, Hormann K, Heinz LX, Boztug K, Superti-Furga G. Cell Death Differ. 2018 Sep 20. pii: 10.1038/s41418-018-0192-6. doi: 10.1038/s41418-018-0192-6. PubMed
PAXX Participates in Base Excision Repair via Interacting with Pol beta and Contributes to TMZ Resistance in Glioma Cells. Yang B, Fu X, Hao J, Sun J, Li Z, Li H, Xu H. J Mol Neurosci. 2018 Oct;66(2):214-221. doi: 10.1007/s12031-018-1157-4. Epub 2018 Sep 20. PubMed
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E, Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. PubMed

Associated Plasmids

Myostatin knockout induces apoptosis in human cervical cancer cells via elevated reactive oxygen species generation. Han YQ, Ming SL, Wu HT, Zeng L, Ba G, Li J, Lu WF, Han J, Du QJ, Sun MM, Yang GY, Wang J, Chu BB. Redox Biol. 2018 Oct;19:412-428. doi: 10.1016/j.redox.2018.09.009. Epub 2018 Sep 13. PubMed
NPC1L1-dependent intestinal cholesterol absorption requires ganglioside GM3 in membrane microdomains. Nihei W, Nagafuku M, Hayamizu H, Odagiri Y, Tamura Y, Kikuchi Y, Veillon L, Kanoh H, Inamori KI, Arai K, Kabayama K, Fukase K, Inokuchi JI. J Lipid Res. 2018 Nov;59(11):2181-2187. doi: 10.1194/jlr.M089201. Epub 2018 Sep 21. PubMed
ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer. Harwood FC, Klein Geltink RI, O'Hara BP, Cardone M, Janke L, Finkelstein D, Entin I, Paul L, Houghton PJ, Grosveld GC. Sci Adv. 2018 Sep 12;4(9):eaar3938. doi: 10.1126/sciadv.aar3938. eCollec