pDY153: Flag(SUZ12) WT HDR plasmid
(Plasmid
#170793)
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PurposeWT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus
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Depositing Lab
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Depositing OrganizationUniversity of Colorado, Boulder
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 170793-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 170793-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepuc19
- Total vector size (bp) 10600
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSUZ12
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SpeciesH. sapiens (human)
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Entrez GeneSUZ12 (a.k.a. CHET9, IMMAS, JJAZ1)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis plasmid was designed by Daniel Youmans but synthesized by GenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 170793-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 170793-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDY153: Flag(SUZ12) WT HDR plasmid was a gift from Thomas Cech (Addgene plasmid # 170793 ; http://n2t.net/addgene:170793 ; RRID:Addgene_170793) -
For your References section:
Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells. Youmans DT, Gooding AR, Dowell RD, Cech TR. Mol Cell. 2021 Feb 4;81(3):488-501.e9. doi: 10.1016/j.molcel.2020.11.044. Epub 2020 Dec 17. 10.1016/j.molcel.2020.11.044 PubMed 33338397
Map uploaded by the depositor.
