pDY153: Flag(SUZ12) WT HDR plasmid
(Plasmid
#170793)
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PurposeWT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepuc19
- Total vector size (bp) 10600
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSUZ12
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SpeciesH. sapiens (human)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis plasmid was designed by Daniel Youmans but synthesized by GenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDY153: Flag(SUZ12) WT HDR plasmid was a gift from Thomas Cech (Addgene plasmid # 170793 ; http://n2t.net/addgene:170793 ; RRID:Addgene_170793) -
For your References section:
Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells. Youmans DT, Gooding AR, Dowell RD, Cech TR. Mol Cell. 2021 Feb 4;81(3):488-501.e9. doi: 10.1016/j.molcel.2020.11.044. Epub 2020 Dec 17. 10.1016/j.molcel.2020.11.044 PubMed 33338397
Map uploaded by the depositor.
