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pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4
(Plasmid #193294)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 193294 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCXLE
  • Backbone size w/o insert (bp) 10180
  • Total vector size (bp) 13653
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry-t2a-SOX2-17-p2a-KLF4
  • Alt name
    superSOX
  • Alt name
    S*
  • Alt name
    KLF4
  • Species
    H. sapiens (human); Discosoma
  • Insert Size (bp)
    3473
  • Mutation
    SOX2-17 is engineered highly cooperative chimeric transcription factor, where the following elements of SOX2 were swapped with SOX17: 43-47, 61, 65-86 and CTD
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer tctgctaaccatgttcatgccttc
  • 3′ sequencing primer AGGAAGGTCCGCTGGATTGA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4 was a gift from Hans Schöler (Addgene plasmid # 193294 ; http://n2t.net/addgene:193294 ; RRID:Addgene_193294)
  • For your References section:

    Enhancing Sox/Oct cooperativity induces higher-grade developmental reset. MacCarthy CM, Malik V, Wu G, Velychko T, Keshet G, Jauch R, Cojocaru V, Schöler HR, Velychko S. bioRxiv 2022.09.23.509242 10.1101/2022.09.23.509242