PurposeExpresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||131670||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5444
- Total vector size (bp) 6821
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert namee18 (engineered RAD18 with SAP domain deleted)
SpeciesH. sapiens (human)
Insert Size (bp)1377
Entrez GeneRAD18 (a.k.a. RNF73)
- Promoter CMV
/ Fusion Proteins
- FLAG (N terminal on backbone)
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1(+)- e18 was a gift from Alberto Ciccia (Addgene plasmid # 131670 ; http://n2t.net/addgene:131670 ; RRID:Addgene_131670)
For your References section:Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant. Nambiar TS, Billon P, Diedenhofen G, Hayward SB, Taglialatela A, Cai K, Huang JW, Leuzzi G, Cuella-Martin R, Palacios A, Gupta A, Egli D, Ciccia A. Nat Commun. 2019 Jul 30;10(1):3395. doi: 10.1038/s41467-019-11105-z. 10.1038/s41467-019-11105-z PubMed 31363085