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Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant.
Nambiar TS, Billon P, Diedenhofen G, Hayward SB, Taglialatela A, Cai K, Huang JW, Leuzzi G, Cuella-Martin R, Palacios A, Gupta A, Egli D, Ciccia A
Nat Commun. 2019 Jul 30;10(1):3395. doi: 10.1038/s41467-019-11105-z.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
131669pFUGW-GFP-2-cut reporter Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining.
131670pcDNA3.1(+)- e18Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.
131729pcDNA3.1(+)- e18 LOFExpresses a loss of function mutant of an engineered variant of RAD18 ("e18")

Recombinant Antibodies from Article