pcDNA3.1(+)- e18 LOF
(Plasmid
#131729)
-
PurposeExpresses a loss of function mutant of an engineered variant of RAD18 ("e18")
-
Depositing Lab
-
Depositing OrganizationColumbia University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131729 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 131729-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 131729-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepcDNA3.1(+)
- Backbone size w/o insert (bp) 5444
- Total vector size (bp) 6821
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namee18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation)
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1377
-
GenBank IDGene ID: 56852
-
Entrez GeneRAD18 (a.k.a. RNF73)
- Promoter CMV
-
Tags
/ Fusion Proteins
- FLAG (N terminal on backbone)
- HA (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV Forward
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byRAD18 gene was obtained from the ORFeome v7 (ID:1782)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 131729-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 131729-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1(+)- e18 LOF was a gift from Alberto Ciccia (Addgene plasmid # 131729 ; http://n2t.net/addgene:131729 ; RRID:Addgene_131729) -
For your References section:
Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant. Nambiar TS, Billon P, Diedenhofen G, Hayward SB, Taglialatela A, Cai K, Huang JW, Leuzzi G, Cuella-Martin R, Palacios A, Gupta A, Egli D, Ciccia A. Nat Commun. 2019 Jul 30;10(1):3395. doi: 10.1038/s41467-019-11105-z. 10.1038/s41467-019-11105-z PubMed 31363085
