PurposeAPRT sgRNA-2 and Cas9 expression vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107274||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAPRT sgRNA-2
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (destroyed during cloning)
- 3′ cloning site BbsI (destroyed during cloning)
- 5′ sequencing primer GAGGGCCTATTTCCCATGATTCC (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pX-APRT-sg2 was a gift from Knut Woltjen (Addgene plasmid # 107274 ; http://n2t.net/addgene:107274 ; RRID:Addgene_107274)
For your References section:Microhomology-assisted scarless genome editing in human iPSCs. Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K. Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y. 10.1038/s41467-018-03044-y PubMed 29507284