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Homology-mediated end joining-based targeted integration using CRISPR/Cas9.
Yao X, Wang X, Hu X, Liu Z, Liu J, Zhou H, Shen X, Wei Y, Huang Z, Ying W, Wang Y, Nie YH, Zhang CC, Li S, Cheng L, Wang Q, Wu Y, Huang P, Sun Q, Shi L, Yang H
Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
97307AAV_Efs_hSpCas9_NLS_FLAG-SV40AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter
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97308AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyAHMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.
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97309AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyAHR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.
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97310AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyANHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.
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97311AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyAMMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.
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97312Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyAHMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.
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97313Lenti_Efs_hSpCas9_NLS_FLAG-WPRELentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.
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97316AAV_Actb NHEJ donorNHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.
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97317AAV_Actb HR donorHR donor for fusing a p2A-mCherry reporter to mouse Actb.
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97318AAV_Actb HMEJ donorHMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.
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97319AAV_Cdx2 HMEJ donorHMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV backbone.
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97320AAV_Dbh HMEJ donorHMEJ donor for fusing a p2A-mCherry-WPRE reporter to mouse Dbh. AAV backbone.
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97321AAV_Nanog HMEJ donorHMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.
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97322AAV_Sox2 HMEJ donorHMEJ donor for fusing a p2A-mCherry reporter to mouse Sox2. AAV backbone.
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