Homology-mediated end joining-based targeted integration using CRISPR/Cas9.
Yao X, Wang X, Hu X, Liu Z, Liu J, Zhou H, Shen X, Wei Y, Huang Z, Ying W, Wang Y, Nie YH, Zhang CC, Li S, Cheng L, Wang Q, Wu Y, Huang P, Sun Q, Shi L, Yang H
Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76. PubMed Article
Yao X, Wang X, Hu X, Liu Z, Liu J, Zhou H, Shen X, Wei Y, Huang Z, Ying W, Wang Y, Nie YH, Zhang CC, Li S, Cheng L, Wang Q, Wu Y, Huang P, Sun Q, Shi L, Yang H
Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 97307 | AAV_Efs_hSpCas9_NLS_FLAG-SV40 | AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter |
| 97308 | AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA | HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone. |
| 97309 | AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA | HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone. |
| 97310 | AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA | NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone. |
| 97311 | AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA | MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone. |
| 97312 | Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA | HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone. |
| 97313 | Lenti_Efs_hSpCas9_NLS_FLAG-WPRE | Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter. |
| 97315 | AAV_Actb MMEJ donor | MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. |
| 97316 | AAV_Actb NHEJ donor | NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. |
| 97317 | AAV_Actb HR donor | HR donor for fusing a p2A-mCherry reporter to mouse Actb. |
| 97318 | AAV_Actb HMEJ donor | HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. |
| 97319 | AAV_Cdx2 HMEJ donor | HMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV backbone. |
| 97320 | AAV_Dbh HMEJ donor | HMEJ donor for fusing a p2A-mCherry-WPRE reporter to mouse Dbh. AAV backbone. |
| 97321 | AAV_Nanog HMEJ donor | HMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone. |
| 97322 | AAV_Sox2 HMEJ donor | HMEJ donor for fusing a p2A-mCherry reporter to mouse Sox2. AAV backbone. |