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px330-TSC1
(Plasmid #254275)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254275 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pX330
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tsc1 gRNA
  • gRNA/shRNA sequence
    CAGTGTGGAGGAGTCCAGCA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tsc1

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    px330-TSC1 was a gift from Angelique Bordey & Lena Nguyen (Addgene plasmid # 254275 ; http://n2t.net/addgene:254275 ; RRID:Addgene_254275)
  • For your References section:

    The mTOR pathway genes MTOR, Rheb, Depdc5, Pten, and Tsc1 have convergent and divergent impacts on cortical neuron development and function. Nguyen LH, Xu Y, Nair M, Bordey A. Elife. 2024 Feb 27;12:RP91010. doi: 10.7554/eLife.91010. 10.7554/eLife.91010 PubMed 38411613