px330-TSC1
(Plasmid
#254275)
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PurposeKnockout TSC1
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254275 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepX330
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
px330-TSC1 was a gift from Angelique Bordey & Lena Nguyen (Addgene plasmid # 254275 ; http://n2t.net/addgene:254275 ; RRID:Addgene_254275) -
For your References section:
The mTOR pathway genes MTOR, Rheb, Depdc5, Pten, and Tsc1 have convergent and divergent impacts on cortical neuron development and function. Nguyen LH, Xu Y, Nair M, Bordey A. Elife. 2024 Feb 27;12:RP91010. doi: 10.7554/eLife.91010. 10.7554/eLife.91010 PubMed 38411613