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Addgene

HA-mEOS3.2-TRF2 HR Donor
(Plasmid #86845)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86845 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFastBac
  • Vector type
    Mammalian Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA-mEOS3.2-TRF2 at endogenous locus
  • Species
    H. sapiens (human)
  • Entrez Gene
    TERF2 (a.k.a. TRBF2, TRF2)
  • Promoter Endogenous TRF2 promoter
  • Tag / Fusion Protein
    • HA-mEOS3.2 (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutation R98K was found in Gentamicin during Addgene's quality control process.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-mEOS3.2-TRF2 HR Donor was a gift from Thomas Cech (Addgene plasmid # 86845 ; http://n2t.net/addgene:86845 ; RRID:Addgene_86845)
  • For your References section:

    Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres. Schmidt JC, Zaug AJ, Cech TR. Cell. 2016 Aug 25;166(5):1188-1197.e9. doi: 10.1016/j.cell.2016.07.033. Epub 2016 Aug 11. 10.1016/j.cell.2016.07.033 PubMed 27523609