HA-mEOS3.2-TRF2 HR Donor
(Plasmid
#86845)
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PurposeHR Donor for N-terminal HA-mEOS3.2-TRF2 fusion at endogenous TRF2 locus in human cells.
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Depositing Lab
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Depositing OrganizationUniversity of Colorado, Boulder
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86845 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 86845-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 86845-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepFastBac
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Vector typeMammalian Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHA-mEOS3.2-TRF2 at endogenous locus
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SpeciesH. sapiens (human)
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Entrez GeneTERF2 (a.k.a. TRBF2, TRF2)
- Promoter Endogenous TRF2 promoter
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Tag
/ Fusion Protein
- HA-mEOS3.2 (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer MBP forward
- 3′ sequencing primer SV40 poly A rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutation R98K was found in Gentamicin during Addgene's quality control process.
DNA (Catalog # 86845-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 86845-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-mEOS3.2-TRF2 HR Donor was a gift from Thomas Cech (Addgene plasmid # 86845 ; http://n2t.net/addgene:86845 ; RRID:Addgene_86845) -
For your References section:
Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres. Schmidt JC, Zaug AJ, Cech TR. Cell. 2016 Aug 25;166(5):1188-1197.e9. doi: 10.1016/j.cell.2016.07.033. Epub 2016 Aug 11. 10.1016/j.cell.2016.07.033 PubMed 27523609
