Selectable one-step PCR-mediated integration of a degron for rapid depletion of endogenous human proteins.
Sheridan RM, Bentley DL
Biotechniques. 2016 Feb 1;60(2):69-74. doi: 10.2144/000114378. eCollection 2016 Feb. PubMed Article
Sheridan RM, Bentley DL
Biotechniques. 2016 Feb 1;60(2):69-74. doi: 10.2144/000114378. eCollection 2016 Feb. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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86395 | pAc5 HA3-eDHFR-T2A-puro | for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection |
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86396 | pAc5 HA3-eDHFR-T2A-neo | for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection |
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