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PurposeDSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98895 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLenti CMV Neo
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Backbone manufacturerEric Campeau
- Backbone size w/o insert (bp) 6700
- Total vector size (bp) 8360
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNeomycin-T2A-ISce1-ISce1-eGFP
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SpeciesSynthetic
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Insert Size (bp)1660
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CGC AAA TGG GCG GTA GGC GTG (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLCN DSB Repair Reporter (DRR) was a gift from Jan Karlseder (Addgene plasmid # 98895 ; http://n2t.net/addgene:98895 ; RRID:Addgene_98895) -
For your References section:
Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN. Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J. Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023. 10.1038/nature24023 PubMed 28959974
Map uploaded by the depositor.
