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Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN.
Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J
Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
98895pLCN DSB Repair Reporter (DRR)DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.
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98896pCAGGS DRR mCherry Donor EF1a BFPHomologous recombination donor for pDRR. Expresses mCherry after repait by HR.
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98970pCAGGS Donor mClover-LMNADonor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter
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98971pUC CBA-SpCas9.EF1a-BFP.sgLMNACas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.
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98972pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.
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98973pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.
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98974pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.
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98975pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.
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98976pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.
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