pCW57.kc1-HSD17B10
(Plasmid
#164076)
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PurposeHuman codon optimized HSD17B10. Expression is controlled by doxycycline.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164076 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCW
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHSD17B10
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SpeciesH. sapiens (human)
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Insert Size (bp)786
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Entrez GeneHSD17B10 (a.k.a. 17b-HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, HSD10MD, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1)
Resource Information
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A portion of this plasmid was derived from a plasmid made bysynthesized from IDT
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCW57.kc1-HSD17B10 was a gift from David Sabatini (Addgene plasmid # 164076 ; http://n2t.net/addgene:164076 ; RRID:Addgene_164076) -
For your References section:
Genome-wide CRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction. Condon KJ, Orozco JM, Adelmann CH, Spinelli JB, van der Helm PW, Roberts JM, Kunchok T, Sabatini DM. Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2022120118. doi: 10.1073/pnas.2022120118. 10.1073/pnas.2022120118 PubMed 33483422
Map uploaded by the depositor.
