pWZ699
(Plasmid
#208117)
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Purpose(Empty Backbone) Payload yeast assembly vector with TK-mActb on backbone
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208117 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneF plasmid
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Backbone manufacturerN/A
- Backbone size (bp) 13000
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Modifications to backboneInserting yeast replication origin, auxotrophic marker LEU2, mouse Actb gene, and thymidine kinase transcription unit.
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Vector typeMammalian Expression, Bacterial Expression, Yeast Expression, Synthetic Biology ; Copy number inducible
- Promoter Human PGK1 for thymidine kinase
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)EPi300
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Growth instructionsAdding 0.2% arabinose for copy number induction.
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWZ699 was a gift from Jef Boeke (Addgene plasmid # 208117 ; http://n2t.net/addgene:208117 ; RRID:Addgene_208117) -
For your References section:
Mouse genome rewriting and tailoring of three important disease loci. Zhang W, Golynker I, Brosh R, Fajardo A, Zhu Y, Wudzinska AM, Ordonez R, Ribeiro-Dos-Santos AM, Carrau L, Damani-Yokota P, Yeung ST, Khairallah C, Vela Gartner A, Chalhoub N, Huang E, Ashe HJ, Khanna KM, Maurano MT, Kim SY, tenOever BR, Boeke JD. Nature. 2023 Nov 1. doi: 10.1038/s41586-023-06675-4. 10.1038/s41586-023-06675-4 PubMed 37914927
Map uploaded by the depositor.
