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(Plasmid #168460)


Item Catalog # Description Quantity Price (USD)
Plasmid 168460 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Maurano and Boeke labs, The Center for Synthetic Regulatory Genomics, NYU Langone Health
  • Vector type
    Synthetic Biology ; Yeast assembly vector for Big-IN payloads
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    TransforMax EPI300
  • Growth instructions
    Copy number induction is recommended for high yield preps. See Lucigen’s growth and copy number induction protocol for TransforMax EPI300 cells.
  • Copy number
    Low Copy


  • Gene/Insert name
  • Insert Size (bp)

Cloning Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLM1110_LEU2 was a gift from Jef Boeke (Addgene plasmid # 168460 ; ; RRID:Addgene_168460)
  • For your References section:

    A versatile platform for locus-scale genome rewriting and verification. Brosh R, Laurent JM, Ordonez R, Huang E, Hogan MS, Hitchcock AM, Mitchell LA, Pinglay S, Cadley JA, Luther RD, Truong DM, Boeke JD, Maurano MT. Proc Natl Acad Sci U S A. 2021 Mar 9;118(10). pii: 2023952118. doi: 10.1073/pnas.2023952118. 10.1073/pnas.2023952118 PubMed 33649239