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(Plasmid #184751)


Item Catalog # Description Quantity Price (USD)
Plasmid 184751 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pLM1110_LEU2 with a HSV-ΔTK counterselectable marker cassette
  • Backbone manufacturer
    The Center for Synthetic Regulatory Genetics at NYU Langone Health
  • Backbone size w/o insert (bp) 11564
  • Total vector size (bp) 14183
  • Vector type
    Mammalian Expression, Mouse Targeting, Cre/Lox, Synthetic Biology ; Recombinase-mediated cassette exchange (RMCE)
  • Selectable markers
    Blasticidin ; HSV1-ΔTK counterselectable backbone marker (driven by a human PGK1 promoter)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    TransforMax EPI300
  • Growth instructions
    Copy number induction is recommended for high yield preps. See Lucigen’s growth and copy number induction protocol for TransforMax EPI300 cells.
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
    Human EF1a promoter driven CDS containing eGFP, T2A peptide and a blasticidin resistance gene. Bovine Growth Hormone Terminator.
  • Species
    H. sapiens (human), B. taurus (bovine)
  • Insert Size (bp)
  • Promoter EF1a

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TGATCTCGCCCCGAGAACTG
  • 3′ sequencing primer TCTATACCCGGGATCCCCAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note plasmid contains an IS1 element. Depositor confirms this is not a concern for function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPL001-BBTK was a gift from Jef Boeke (Addgene plasmid # 184751 ; ; RRID:Addgene_184751)
  • For your References section:

    A versatile platform for locus-scale genome rewriting and verification. Brosh R, Laurent JM, Ordonez R, Huang E, Hogan MS, Hitchcock AM, Mitchell LA, Pinglay S, Cadley JA, Luther RD, Truong DM, Boeke JD, Maurano MT. Proc Natl Acad Sci U S A. 2021 Mar 9;118(10). pii: 2023952118. doi: 10.1073/pnas.2023952118. 10.1073/pnas.2023952118 PubMed 33649239