In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining.
Geisinger JM, Turan S, Hernandez S, Spector LP, Calos MP
Nucleic Acids Res. 2016 Jan 13. pii: gkv1542. PubMed Article
Geisinger JM, Turan S, Hernandez S, Spector LP, Calos MP
Nucleic Acids Res. 2016 Jan 13. pii: gkv1542. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 75380 | pX330-BsaIx2-DD-Cas9 | Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1 |
| 85974 | pKER-Clover | Expresses Clover from the EF1a promoter in mammalian cells |
| 85975 | pKER-mKOk | Expresses mKOk from the EF1a promoter in mammalian cells |
| 85976 | pKER-mCardinal | Expresses mCardinal from the EF1a promoter in mammalian cells |
| 85977 | pKER-mRuby2 | Expresses mRuby2 from the EF1a promoter in mammalian cells |
| 85978 | pX330-H11r1-2-DD-Cas9 | Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells |
| 86622 | pKER-mCerulean | Expresses mCerulean from the EF1a promoter in mammalian cells |