The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.
Ishimura R, El-Gowily AH, Noshiro D, Komatsu-Hirota S, Ono Y, Shindo M, Hatta T, Abe M, Uemura T, Lee-Okada HC, Mohamed TM, Yokomizo T, Ueno T, Sakimura K, Natsume T, Sorimachi H, Inada T, Waguri S, Noda NN, Komatsu M
Nat Commun. 2022 Dec 21;13(1):7857. doi: 10.1038/s41467-022-35501-0. PubMed Article
Ishimura R, El-Gowily AH, Noshiro D, Komatsu-Hirota S, Ono Y, Shindo M, Hatta T, Abe M, Uemura T, Lee-Okada HC, Mohamed TM, Yokomizo T, Ueno T, Sakimura K, Natsume T, Sorimachi H, Inada T, Waguri S, Noda NN, Komatsu M
Nat Commun. 2022 Dec 21;13(1):7857. doi: 10.1038/s41467-022-35501-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204551 | pHis-3xFLAG-CMV14-CYB5R3 | Expresses 3xFLAG-tagged CYB5R3 in mammalian cells |
| 204552 | pHis-3xFLAG-CMV14-CYB5R3 K214R | Expresses 3xFLAG-tagged CYB5R3 K214R in mammalian cells |
| 204553 | pIRESpuroN3xFLAG-UFSP2 | Expresses 3xFLAG-tagged UFSP2 in mammalian cells |
| 204554 | pIRESpuroN3xFLAG-UFSP2 C302A | Expresses 3xFLAG-tagged UFSP2 C302A in mammalian cells |
| 204555 | pIRESpuroN3xFLAG-His-CDK5RAP3 | Expresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cells |
| 204556 | pcDNA3(+)-UFBP1 F196A V198A-MYC | Expresses FLAG-tagged UFBP1 F196A V198A in mammalian cells |
| 204557 | pGEX6p-1-CYB5R3 deltaN26 | Expresses GST-tagged CYB5R3 deltaN26 in E.coli |
| 204558 | pGEX6p-1-UFBP1 | Expresses GST-tagged UFBP1 in E.coli |
| 204559 | pGEX6p-1-UFBP1 M1 | Expresses GST-tagged UFBP1 M1 in E.coli |
| 204560 | pGEX6p-1-UFBP1 M2 | Expresses GST-tagged UFBP1 M2 in E.coli |
| 204561 | pGEX6p-1-UFBP1 M3 | Expresses GST-tagged UFBP1 M3 in E.coli |
| 204562 | pGEX6p-1-UFBP1 M4 | Expresses GST-tagged UFBP1 M4 in E.coli |
| 204563 | pGEX6p-1-UFBP1 M5 | Expresses GST-tagged UFBP1 M5 in E.coli |
| 204564 | pGEX6p-1-UFBP1 F196A V198A | Expresses GST-tagged UFBP1 F196A V198A in E.coli |
| 204565 | pGEX6p-1-OSF-UFM1 | Expresses GST-Strep-FLAG-tagged UFM1 in E.coli |
| 204566 | pGEX6p-1-CDK5RAP3 | Expresses GST-CDK5RAP3 in E.coli |
| 204567 | pEGFP-N3-CYB5R3 | Expresses EGFP-tagged CYB5R3 in mammalian cells |
| 204568 | pEGFP-N3-CYB5R3 deltaN26 | Expresses EGFP-tagged CYB5R3 deltaN26 in mammalian cells |
| 204569 | pmCherry-EGFP-N3-CYB5R3 | Expresses mCherry-EGFP-tagged CYB5R3 in mammalian cells |
| 204570 | pmCherry-EGFP-N3-CYB5R3 K214R | Expresses mCherry-EGFP-tagged CYB5R3 K214R in mammalian cells |
| 204571 | phAG-MCL-CDK5RAP3 | Expresses hAG-tagged CDK5RAP3 in mammalian cells |
| 204572 | phAG-MCL-CDK5RAP3 W269A W294A W312A | Expresses hAG-tagged CDK5RAP3 W269A W294A W312A in mammalian cells |
| 204573 | pAsh-MCL-LC3B | Expresses Ash-tagged LC3B in mammalian cells |
| 204574 | pAsh-MCL-GABARAP | Expresses Ash-tagged GABARAP in mammalian cells |