px330-CD4
(Plasmid #136938)

• Purpose: NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH
• Depositing Lab: Jeremy Stark
• Publication: Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010421. doi: 10.1074/jbc.RA119.010421.

Backbone

• Vector backbone: px330
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: sgRNA targeting CD4
• gRNA/shRNA sequence: GGCGTATCTGTGTGAGGACT
• Species: H. sapiens (human)
• Entrez Gene: CD4 (a.k.a. CD4mut, IMD79, Leu-3, OKT4D, T4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bbs1 (destroyed during cloning)
• 3′ cloning site: Bbs1 (destroyed during cloning)
• 5′ sequencing primer: GACTATCATATGCTTACCGT

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry