HR180-LGR5-CreER
(Plasmid #129093)

• Purpose: KI CreER with a puromycin/mRuby selection cassette into human LGR5 locus
• Depositing Lab: Toshiro Sato
• Publication: Shimokawa et al Nature. 2017 May 11;545(7653):187-192. doi: 10.1038/nature22081. Epub 2017 Mar 29.

Backbone

• Vector backbone: HR180-PA
• Vector type: homologous recombination donor plasmid
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CreERT2 and LGR5 homology arms
• Species: H. sapiens (human)
• Entrez Gene: LGR5 (a.k.a. FEX, GPR49, GPR67, GRP49, HG38)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • HR180-LGR5-CreER.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Includes 5' and 3' LGR5 homologous arms.

mRuby is a part of the selection marker mRuby T2A puro, allowing researchers to visually check whether KI is successful.