Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.
Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14. PubMed Article
Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124166 | KA0637_pPBCAG-rtTAM2-IN | piggyBac-based expression of rtTA-M2 |
| 124167 | KA1775_pPBCAG-rtTAM2-IH | piggyBac-based expression of rtTA-M2 |
| 124168 | KA0717_pPB-hCMV*1-cHA-IRESVenus | piggyBac-based Dox-inducible expression |
| 124169 | KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2 | piggyBac-based Dox-inducible expression |
| 124170 | KA1065_pPB-hCMV*1-cHA-IRESH2BmCherry | piggyBac-based Dox-inducible expression |
| 124171 | KA0718_pPB-TREtight-cHA-IRESVenus | piggyBac-based Dox-inducible expression |
| 124172 | KA1123_pPB-TREtight-cHA-IRESH2BmKO2 | piggyBac-based Dox-inducible expression |
| 124173 | KA1130_pPB-TREtight-cHA-IRESH2BmCherry | piggyBac-based Dox-inducible expression |
| 124174 | KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2 | piggyBac-based Dox-inducible expression (weak) |
| 124175 | KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2 | piggyBac-based Dox-inducible expression (very weak) |
| 124176 | KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2 | piggyBac-based Dox-inducible expression (moderate) |
| 124177 | KA1042_pPB-hCMV*1-Esrrb-IV | piggyBac-based Dox-inducible expression of Esrrb |
| 124178 | KA2880_pPB-TREtight-FLAG-Esrrb-InO | piggyBac-based Dox-inducible expression of FLAG-Esrrb |
| 124179 | KA2944_pPB-TREtight-Esrrb-FLAG-InO | piggyBac-based Dox-inducible expression of Esrrb-FLAG |
| 124180 | KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP | piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer |
| 124181 | KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP | piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer |
| 124183 | KA1153_pPBCAG-MerCreMer-IN | piggyBac-based expression of the tamoxifen-inducible Cre |
| 124184 | KA1158_pPBCAG-MerCreMer-IZ | piggyBac-based expression of the tamoxifen-inducible Cre |
| 124185 | KA1390_pGL4-UBCmin-luc2 | Luciferase reporter with the UBC minimal promoter |
| 124186 | KA1395_pGL4-UBCmin-luc2/Klf4-B' | Luciferase reporter driven by the Klf4 enhancer |
| 124187 | KA1396_pGL4-UBCmin-luc2/Klf4-E' | Luciferase reporter driven by the Klf4 enhancer |
| 124188 | KA1401_pGL4-UBCmin-luc2/Klf4-F' | Luciferase reporter driven by the Klf4 enhancer |
| 124189 | KA1402_pGL4-UBCmin-luc2/Nanog-A' | Luciferase reporter driven by the Nanog enhancer |
| 124190 | KA1403_pGL4-UBCmin-luc2/Nanog-B' | Luciferase reporter driven by the Nanog enhancer |
| 124191 | KA1865_pGL4-UBCmin-luc2/Pou5f1_CR4 | Luciferase reporter driven by the Pou5f1 enhancer |
| 124192 | KA1866_pGL4-UBCmin-luc2/Pou5f1_CR3 | Luciferase reporter driven by the Pou5f1 enhancer |
| 124193 | KA1867_pGL4-UBCmin-luc2/Pou5f1_CR2 | Luciferase reporter driven by the Pou5f1 enhancer |
| 124194 | KA1868_pGL4-UBCmin-luc2/Utf1-A' | Luciferase reporter driven by the Utf1 enhancer |
| 124195 | KA1869_pGL4-UBCmin-luc2/Fgf4-A' | Luciferase reporter driven by the Fgf4 enhancer |
| 124196 | KA1870_pGL4-UBCmin-luc2/Esrrb-B' | Luciferase reporter driven by the Esrrb enhancer |
| 124197 | KA1874_pGL4-UBCmin-luc2/Sox2_SRR1 | Luciferase reporter driven by the Sox2 enhancer |
| 124198 | KA1875_pGL4-UBCmin-luc2/Sox2_SRR2 | Luciferase reporter driven by the Sox2 enhancer |
| 124199 | KA2626_pGL4-UBCmin-luc2/Klf2-D' | Luciferase reporter driven by the Klf2 enhancer |
| 124200 | KA2627_pGL4-UBCmin-luc2/Jam2-A' | Luciferase reporter driven by the Jam2 enhancer |
| 124201 | KA2628_pGL4-UBCmin-luc2/Tbx3-B' | Luciferase reporter driven by the Tbx3 enhancer |
| 124202 | KA2058_pX330A-G2P | Expression of SpCas9 and sgRNA |
| 124203 | KA2601_eSpCas9-G2P | Expression of eSpCas9(1.1) and sgRNA |
| 124204 | KA2706_eSpCas9-PGKPacdtkCh | Expression of eSpCas9(1.1) and sgRNA |
| 124205 | KA2963_eSpCas9-PGKHygdtkCh | Expression of eSpCas9(1.1) and sgRNA |