Cdk12_intron3_sg2_pX330
(Plasmid #127174)

• Purpose: sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone
• Depositing Lab: Phil Sharp
• Publication: Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi: 10.1038/s41586-018-0758-y. Epub 2018 Nov 28.

Backbone

• Vector backbone: pX330
• Backbone manufacturer: Feng Zhang Lab (Addgene Plasmid #42230)
• Backbone size w/o insert (bp): 8484
• Total vector size (bp): 8509
• Modifications to backbone: gRNA sequence provided cloned into pX330 using modified version of protocol published in https://www.nature.com/articles/nprot.2013.143
• Vector type: Mouse Targeting, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mouse Cdk12 Intron 3 sgRNA
• gRNA/shRNA sequence: TCGAGGCCAGCCTAGTCTAG
• Species: M. musculus (mouse)
• Promoter: U6 Promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: LKO.1 5' primer (gactatcatatgcttaccgt)

Resource Information

• Supplemental Documents:
  • Cdk12_intron3_sg2_pX330.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry