Addgene: Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing.

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Thomas Cech
Goldfarb et al

Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing.
Goldfarb KC, Cech TR
Genes Dev. 2017 Jan 1;31(1):59-71. doi: 10.1101/gad.286963.116. Epub 2017 Jan 23. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
87189	px330-MRPsg1	disruption of human RMRP gene via CRISPR-Cas9, guide 1
87190	px330-MRPsg2	disruption of human RMRP gene via CRISPR-Cas9, guide 2
87191	px330-MRPsg3	disruption of human RMRP gene via CRISPR-Cas9, guide 3
87192	px330-MRPsg4	disruption of human RMRP gene via CRISPR-Cas9, guide 4
87193	psglessClonePX	used for downstream cloning, contains CMV promoter with NheI and XhoI cloning sites flanking a partial insert that gets excised when adding an insert of choice
87194	p_3xFLAG-Rpp25	Expression of ectopic 3xFLAG-Rpp25 construct from CMV promoter
87199	p-715MRPprom-MRPcucuc	Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

Antibodies from Article