Skip to main content
Addgene
Open Menu

Wild-type-ThermoCas9-3xNLS
(Plasmid #254685)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254685 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pML-1B
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    See manuscript. Use Rosetta DE3 for expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Wildtype ThermoCas9
  • Species
    Geobacillus thermodenitrificans strain T12
  • GenBank ID
    CP020030
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
    • nucleoplasmin NLS (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Despoina Trasanidou, Christian Sudfeld, Eugenios Bouzetos, Yuerong Shu, Yu Zhao, John van der Oost

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Wild-type-ThermoCas9-3xNLS was a gift from Hong Li (Addgene plasmid # 254685 ; http://n2t.net/addgene:254685 ; RRID:Addgene_254685)

  • For your References section:

    Molecular basis for methylation-sensitive editing by Cas9. Roth MO, Shu Y, Zhao Y, Trasanidou D, Hoffman RD, Sudfeld C, Bouzetos E, Trasanidis N, Zawrotny M, Gelasco MK, Medina ML, Das A, Rai J, Goswami HN, Wang B, van der Oost J, Li H. Nature. 2026 Apr 15. doi: 10.1038/s41586-026-10384-z. 10.1038/s41586-026-10384-z PubMed 41986708
Related items: