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Efficient scarless genome editing in human pluripotent stem cells.
Ikeda K, Uchida N, Nishimura T, White J, Martin RM, Nakauchi H, Sebastiano V, Weinberg KI, Porteus MH
Nat Methods. 2018 Dec;15(12):1045-1047. doi: 10.1038/s41592-018-0212-y. Epub 2018 Nov 30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
123661pSLHDR01_mCherry_tCD19Construction of 1st donor for scarless genome editing
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124061pSLHDR02_EGFP_tCD8Construction of 1st donor for scarless genome editing
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124062pSLHDR03_mCherry_tCD19_unique guideConstruction of 1st donor for scarless genome editing with unique guide
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124209pSLHDR_R1gRNA/Cas9 expression for 2nd editing using pSLHDR01, 02
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124211pSLHDR_R4gRNA/Cas9 expression for 2nd editing using pSLHDR04
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