pCAG-mTOR-S2215Y
(Plasmid
#254274)
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PurposeExpresses mTOR-S2215Y (activating mutation)
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254274 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMTOR-S2215Y
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SpeciesH. sapiens (human)
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MutationChanged Serine 2215 to Tyrosine
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Entrez GeneMTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-mTOR-S2215Y was a gift from Angelique Bordey & Lena Nguyen (Addgene plasmid # 254274 ; http://n2t.net/addgene:254274 ; RRID:Addgene_254274) -
For your References section:
The mTOR pathway genes MTOR, Rheb, Depdc5, Pten, and Tsc1 have convergent and divergent impacts on cortical neuron development and function. Nguyen LH, Xu Y, Nair M, Bordey A. Elife. 2024 Feb 27;12:RP91010. doi: 10.7554/eLife.91010. 10.7554/eLife.91010 PubMed 38411613