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pSpCas9(BB)-2A-Puro (PX459) V2.0 Citations (516)

Plasmid Article: Genome engineering using the CRISPR-Cas9 system.
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
PubMed Journal

Articles Citing pSpCas9(BB)-2A-Puro (PX459) V2.0

Articles
A complex of ZO-1 and the BAR Domain Protein TOCA-1 Regulates Actin Assembly at the Tight Junction. Van Itallie CM, Tietgens AJ, Krystofiak E, Kachar B, Anderson JM. Mol Biol Cell. 2015 Jun 10. pii: mbc.E15-04-0232. PubMed
Generation of a conditional analog-sensitive kinase in human cells using CRISPR/Cas9-mediated genome engineering. Moyer TC, Holland AJ. Methods Cell Biol. 2015;129:19-36. doi: 10.1016/bs.mcb.2015.03.017. Epub 2015 May 27. PubMed
Mechanisms of Enhancer-mediated Hormonal Control of Vitamin D Receptor Gene Expression in Target Cells. Lee SM, Meyer MB, Benkusky NA, O'Brien CA, Pike JW. J Biol Chem. 2015 Dec 18;290(51):30573-86. doi: 10.1074/jbc.M115.693614. Epub 2015 Oct 25. PubMed
Improved methods of AAV-mediated gene targeting for human cell lines using ribosome-skipping 2A peptide. Karnan S, Ota A, Konishi Y, Wahiduzzaman M, Hosokawa Y, Konishi H. Nucleic Acids Res. 2015 Dec 10. pii: gkv1338. PubMed

Associated Plasmids

PAFAH1B1 and the lncRNA NONHSAT073641 maintain an angiogenic phenotype in human endothelial cells. Josipovic I, Fork C, Preussner J, Prior KK, Iloska D, Vasconez AE, Labocha S, Angioni C, Thomas D, Ferreiros N, Looso M, Pullamsetti SS, Geisslinger G, Steinhilber D, Brandes RP, Leisegang MS. Acta Physiol (Oxf). 2016 Apr 28. doi: 10.1111/apha.12700. PubMed

Associated Plasmids

Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9. Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS. PLoS One. 2016 Apr 29;11(4):e0154604. doi: 10.1371/journal.pone.0154604. eCollection 2016. PubMed

Associated Plasmids

Tumour resistance in induced pluripotent stem cells derived from naked mole-rats. Miyawaki S, Kawamura Y, Oiwa Y, Shimizu A, Hachiya T, Bono H, Koya I, Okada Y, Kimura T, Tsuchiya Y, Suzuki S, Onishi N, Kuzumaki N, Matsuzaki Y, Narita M, Ikeda E, Okanoya K, Seino K, Saya H, Okano H, Miura K. Nat Commun. 2016 May 10;7:11471. doi: 10.1038/ncomms11471. PubMed
Glutathione adducts induced by ischemia and deletion of glutaredoxin-1 stabilize HIF-1alpha and improve limb revascularization. Watanabe Y, Murdoch CE, Sano S, Ido Y, Bachschmid MM, Cohen RA, Matsui R. Proc Natl Acad Sci U S A. 2016 May 24;113(21):6011-6. doi: 10.1073/pnas.1524198113. Epub 2016 May 9. PubMed
Golden Gate Assembly of CRISPR gRNA expression array for simultaneously targeting multiple genes. Vad-Nielsen J, Lin L, Bolund L, Nielsen AL, Luo Y. Cell Mol Life Sci. 2016 May 13. PubMed

Associated Plasmids

Genome engineering in ophthalmology: Application of CRISPR/Cas to the treatment of eye disease. Hung SS, McCaughey T, Swann O, Pebay A, Hewitt AW. Prog Retin Eye Res. 2016 Jul;53:1-20. doi: 10.1016/j.preteyeres.2016.05.001. Epub 2016 May 13. PubMed
The BRPF2/BRD1-MOZ complex is involved in retinoic acid-induced differentiation of embryonic stem cells. Cho HI, Kim MS, Jang YK. Exp Cell Res. 2016 May 30. pii: S0014-4827(16)30140-9. doi: 10.1016/j.yexcr.2016.05.022. PubMed
Cell surface protease activation during RAS transformation: Critical role of the plasminogen receptor, S100A10. Madureira PA, Bharadwaj AG, Bydoun M, Garant K, O'Connell P, Lee P, Waisman DM. Oncotarget. 2016 Jul 26;7(30):47720-47737. doi: 10.18632/oncotarget.10279. PubMed
The Influenza A Virus Genotype Determines the Antiviral Function of NF-kappaB. Dam S, Kracht M, Pleschka S, Schmitz ML. J Virol. 2016 Aug 12;90(17):7980-90. doi: 10.1128/JVI.00946-16. Print 2016 Sep 1. PubMed
AAV-Mediated CRISPR/Cas Gene Editing of Retinal Cells In Vivo. Hung SS, Chrysostomou V, Li F, Lim JK, Wang JH, Powell JE, Tu L, Daniszewski M, Lo C, Wong RC, Crowston JG, Pebay A, King AE, Bui BV, Liu GS, Hewitt AW. Invest Ophthalmol Vis Sci. 2016 Jun 1;57(7):3470-6. doi: 10.1167/iovs.16-19316. PubMed

Associated Plasmids

A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Zhang R, Miner JJ, Gorman MJ, Rausch K, Ramage H, White JP, Zuiani A, Zhang P, Fernandez E, Zhang Q, Dowd KA, Pierson TC, Cherry S, Diamond MS. Nature. 2016 Jul 7;535(7610):164-8. doi: 10.1038/nature18625. Epub 2016 Jun 17. PubMed
TREM2 deficiency reduces the efficacy of immunotherapeutic amyloid clearance. Xiang X, Werner G, Bohrmann B, Liesz A, Mazaheri F, Capell A, Feederle R, Knuesel I, Kleinberger G, Haass C. EMBO Mol Med. 2016 Sep 1;8(9):992-1004. doi: 10.15252/emmm.201606370. PubMed
An Evolutionary Conserved Epigenetic Mark of Polycomb Response Elements Implemented by Trx/MLL/COMPASS. Rickels R, Hu D, Collings CK, Woodfin AR, Piunti A, Mohan M, Herz HM, Kvon E, Shilatifard A. Mol Cell. 2016 Jul 21;63(2):318-328. doi: 10.1016/j.molcel.2016.06.018. PubMed
Generation of Targeted Genomic Deletions Through CRISPR/Cas System in Zebrafish. Xiao A, Zhang B. Methods Mol Biol. 2016;1451:65-79. doi: 10.1007/978-1-4939-3771-4_5. PubMed
CRISPR/Cas9-Mediated Trp53 and Brca2 Knockout to Generate Improved Murine Models of Ovarian High-Grade Serous Carcinoma. Walton J, Blagih J, Ennis D, Leung E, Dowson S, Farquharson M, Tookman LA, Orange C, Athineos D, Mason S, Stevenson D, Blyth K, Strathdee D, Balkwill FR, Vousden K, Lockley M, McNeish IA. Cancer Res. 2016 Oct 15;76(20):6118-6129. Epub 2016 Aug 16. PubMed
Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Santos DP, Kiskinis E, Eggan K, Merkle FT. Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.6.1-5B.6.60. doi: 10.1002/cpsc.15. PubMed
Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific heterozygous mutation in CHMP2B. Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, Freude KK. Stem Cell Res. 2016 Jun 16;17(1):148-150. doi: 10.1016/j.scr.2016.06.004. PubMed
Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific homozygous mutation in CHMP2B. Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, Freude KK. Stem Cell Res. 2016 Jun 16;17(1):151-153. doi: 10.1016/j.scr.2016.06.005. PubMed
CRISPR/Cas9-mediated knockout of p22phox leads to loss of Nox1 and Nox4, but not Nox5 activity. Prior KK, Leisegang MS, Josipovic I, Lowe O, Shah AM, Weissmann N, Schroder K, Brandes RP. Redox Biol. 2016 Oct;9:287-295. doi: 10.1016/j.redox.2016.08.013. Epub 2016 Aug 24. PubMed
Mutagenesis and Genome Engineering of Epstein-Barr Virus in Cultured Human Cells by CRISPR/Cas9. Yuen KS, Chan CP, Kok KH, Jin DY. Methods Mol Biol. 2017;1498:23-31. PubMed
Activation of the ATR kinase by the RPA-binding protein ETAA1. Haahr P, Hoffmann S, Tollenaere MA, Ho T, Toledo LI, Mann M, Bekker-Jensen S, Raschle M, Mailand N. Nat Cell Biol. 2016 Nov;18(11):1196-1207. doi: 10.1038/ncb3422. Epub 2016 Oct 10. PubMed
Super-resolution imaging of fluorescently labeled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing. Cho WK, Jayanth N, Mullen S, Tan TH, Jung YJ, Cisse II. Sci Rep. 2016 Oct 26;6:35949. doi: 10.1038/srep35949. PubMed
Generation of a gene-corrected isogenic control hiPSC line derived from a familial Alzheimer's disease patient carrying a L150P mutation in presenilin 1. Poon A, Schmid B, Pires C, Nielsen TT, Hjermind LE, Nielsen JE, Holst B, Hyttel P, Freude KK. Stem Cell Res. 2016 Sep 24;17(3):466-469. doi: 10.1016/j.scr.2016.09.018. PubMed
Generation of an isogenic, gene-corrected iPSC line from a symptomatic 57-year-old female patient with frontotemporal dementia caused by a P301L mutation in the microtubule associated protein tau (MAPT) gene. Nimsanor N, Kitiyanant N, Poulsen U, Rasmussen MA, Clausen C, Mau-Holzmann UA, Nielsen JE, Nielsen TT, Hyttel P, Holst B, Schmid B. Stem Cell Res. 2016 Sep 28;17(3):556-559. doi: 10.1016/j.scr.2016.09.021. PubMed
Control of apico-basal epithelial polarity by the microtubule minus-end-binding protein CAMSAP3 and spectraplakin ACF7. Noordstra I, Liu Q, Nijenhuis W, Hua S, Jiang K, Baars M, Remmelzwaal S, Martin M, Kapitein LC, Akhmanova A. J Cell Sci. 2016 Nov 15;129(22):4278-4288. Epub 2016 Oct 17. PubMed
FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis. Skau CT, Fischer RS, Gurel P, Thiam HR, Tubbs A, Baird MA, Davidson MW, Piel M, Alushin GM, Nussenzweig A, Steeg PS, Waterman CM. Cell. 2016 Dec 1;167(6):1571-1585.e18. doi: 10.1016/j.cell.2016.10.023. Epub 2016 Nov 10. PubMed
A Novel Regulatory Mechanism of Type II Collagen Expression via a SOX9-dependent Enhancer in Intron 6. Yasuda H, Oh CD, Chen D, de Crombrugghe B, Kim JH. J Biol Chem. 2017 Jan 13;292(2):528-538. doi: 10.1074/jbc.M116.758425. Epub 2016 Nov 23. PubMed
Metabolic reprogramming and AMPKalpha1 pathway activation by caulerpin in colorectal cancer cells. Yu H, Zhang H, Dong M, Wu Z, Shen Z, Xie Y, Kong Z, Dai X, Xu B. Int J Oncol. 2017 Jan;50(1):161-172. doi: 10.3892/ijo.2016.3794. Epub 2016 Dec 6. PubMed
Generation of an isogenic, gene-corrected iPSC line from a symptomatic 59-year-old female patient with frontotemporal dementia caused by an R406W mutation in the microtubule associated protein tau (MAPT) gene. Nimsanor N, Poulsen U, Rasmussen MA, Clausen C, Mau-Holzmann UA, Nielsen JE, Nielsen TT, Hyttel P, Holst B, Schmid B. Stem Cell Res. 2016 Sep 28;17(3):576-579. doi: 10.1016/j.scr.2016.09.020. PubMed
Generation of an isogenic, gene-corrected iPSC line from a pre-symptomatic 28-year-old woman with an R406W mutation in the microtubule associated protein tau (MAPT) gene. Nimsanor N, Poulsen U, Rasmussen MA, Clausen C, Mau-Holzmann UA, Nielsen JE, Nielsen TT, Hyttel P, Holst B, Schmid B. Stem Cell Res. 2016 Sep 28;17(3):600-602. doi: 10.1016/j.scr.2016.09.024. PubMed
Visualizing the dynamic coupling of claudin strands to the actin cytoskeleton through ZO-1. Van Itallie CM, Tietgens AJ, Anderson JM. Mol Biol Cell. 2016 Dec 14. pii: mbc.E16-10-0698. PubMed
Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization. Moon S, Kim W, Kim S, Kim Y, Song Y, Bilousov O, Kim J, Lee T, Cha B, Kim M, Kim H, Katanaev VL, Jho EH. EMBO Rep. 2017 Jan;18(1):61-71. doi: 10.15252/embr.201642683. Epub 2016 Dec 15. PubMed
The Effector Cig57 Hijacks FCHO-Mediated Vesicular Trafficking to Facilitate Intracellular Replication of Coxiella burnetii. Latomanski EA, Newton P, Khoo CA, Newton HJ. PLoS Pathog. 2016 Dec 21;12(12):e1006101. doi: 10.1371/journal.ppat.1006101. eCollection 2016 Dec. PubMed
CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins. Van Nostrand EL, Gelboin-Burkhart C, Wang R, Pratt GA, Blue SM, Yeo GW. Methods. 2016 Dec 18. pii: S1046-2023(16)30481-9. doi: 10.1016/j.ymeth.2016.12.007. PubMed
Reduced IL-37 Production Increases Spontaneous Chemokine Expressions in Colon Epithelial Cells. Gunaltay S, Ghiboub M, Hultgren O, Hornquist EH. Dig Dis Sci. 2017 May;62(5):1204-1215. doi: 10.1007/s10620-016-4422-9. Epub 2017 Jan 2. PubMed
Blood pressure-associated polymorphism controls ARHGAP42 expression via serum response factor DNA binding. Bai X, Mangum KD, Dee RA, Stouffer GA, Lee CR, Oni-Orisan A, Patterson C, Schisler JC, Viera AJ, Taylor JM, Mack CP. J Clin Invest. 2017 Feb 1;127(2):670-680. doi: 10.1172/JCI88899. Epub 2017 Jan 23. PubMed
The evolutionary capacitor HSP90 buffers the regulatory effects of mammalian endogenous retroviruses. Hummel B, Hansen EC, Yoveva A, Aprile-Garcia F, Hussong R, Sawarkar R. Nat Struct Mol Biol. 2017 Mar;24(3):234-242. doi: 10.1038/nsmb.3368. Epub 2017 Jan 30. PubMed
Platelet activating factor-induced expression of p21 is correlated with histone acetylation. Damiani E, Puebla-Osorio N, Lege BM, Liu J, Neelapu SS, Ullrich SE. Sci Rep. 2017 Feb 3;7:41959. doi: 10.1038/srep41959. PubMed
Interaction Landscape of Inherited Polymorphisms with Somatic Events in Cancer. Carter H, Marty R, Hofree M, Gross AM, Jensen J, Fisch KM, Wu X, DeBoever C, Van Nostrand EL, Song Y, Wheeler E, Kreisberg JF, Lippman SM, Yeo GW, Gutkind JS, Ideker T. Cancer Discov. 2017 Apr;7(4):410-423. doi: 10.1158/2159-8290.CD-16-1045. Epub 2017 Feb 10. PubMed
A fast method for analyzing essential protein mutants in human cells. Dietsch F, Donzeau M, Cordonnier AM, Weiss E, Chatton B, Vigneron M. Biotechniques. 2017 Feb 1;62(2):80-82. doi: 10.2144/000114518. PubMed
Pervasive translational regulation of the cell signalling circuitry underlies mammalian development. Fujii K, Shi Z, Zhulyn O, Denans N, Barna M. Nat Commun. 2017 Feb 14;8:14443. doi: 10.1038/ncomms14443. PubMed
Systematic in vivo inactivation of chromatin regulating enzymes identifies Setd2 as a potent tumor suppressor in lung adenocarcinoma. Walter DM, Venancio OS, Buza EL, Tobias JW, Deshpande C, Gudiel AA, Kim-Kiselak C, Cicchini M, Yates TJ, Feldser DM. Cancer Res. 2017 Feb 15. pii: canres.2159.2016. doi: 10.1158/0008-5472.CAN-16-2159. PubMed

Associated Plasmids

Patient iPSC-Derived Neurons for Disease Modeling of Frontotemporal Dementia with Mutation in CHMP2B. Zhang Y, Schmid B, Nikolaisen NK, Rasmussen MA, Aldana BI, Agger M, Calloe K, Stummann TC, Larsen HM, Nielsen TT, Huang J, Xu F, Liu X, Bolund L, Meyer M, Bak LK, Waagepetersen HS, Luo Y, Nielsen JE, Holst B, Clausen C, Hyttel P, Freude KK. Stem Cell Reports. 2017 Mar 14;8(3):648-658. doi: 10.1016/j.stemcr.2017.01.012. Epub 2017 Feb 16. PubMed
CTCF participates in DNA damage response via poly(ADP-ribosyl)ation. Han D, Chen Q, Shi J, Zhang F, Yu X. Sci Rep. 2017 Mar 6;7:43530. doi: 10.1038/srep43530. PubMed
Sensing of Bacterial Cyclic Dinucleotides by the Oxidoreductase RECON Promotes NF-kappaB Activation and Shapes a Proinflammatory Antibacterial State. McFarland AP, Luo S, Ahmed-Qadri F, Zuck M, Thayer EF, Goo YA, Hybiske K, Tong L, Woodward JJ. Immunity. 2017 Mar 21;46(3):433-445. doi: 10.1016/j.immuni.2017.02.014. PubMed
DHX9 suppresses RNA processing defects originating from the Alu invasion of the human genome. Aktas T, Avsar Ilik I, Maticzka D, Bhardwaj V, Pessoa Rodrigues C, Mittler G, Manke T, Backofen R, Akhtar A. Nature. 2017 Apr 6;544(7648):115-119. doi: 10.1038/nature21715. Epub 2017 Mar 29. PubMed
Dissection of ubiquitinated protein degradation by basal autophagy. Takayama K, Matsuura A, Itakura E. FEBS Lett. 2017 May;591(9):1199-1211. doi: 10.1002/1873-3468.12641. Epub 2017 Apr 18. PubMed
Psip1/p52 regulates posterior Hoxa genes through activation of lncRNA Hottip. Pradeepa MM, McKenna F, Taylor GC, Bengani H, Grimes GR, Wood AJ, Bhatia S, Bickmore WA. PLoS Genet. 2017 Apr 6;13(4):e1006677. doi: 10.1371/journal.pgen.1006677. eCollection 2017 Apr. PubMed
Proteolysis of mature HIV-1 p6 Gag protein by the insulin-degrading enzyme (IDE) regulates virus replication in an Env-dependent manner. Hahn F, Schmalen A, Setz C, Friedrich M, Schlosser S, Kolle J, Spranger R, Rauch P, Fraedrich K, Reif T, Karius-Fischer J, Balasubramanyam A, Henklein P, Fossen T, Schubert U. PLoS One. 2017 Apr 7;12(4):e0174254. doi: 10.1371/journal.pone.0174254. eCollection 2017. PubMed
Generating stable cell lines with quantifiable protein production using CRISPR/Cas9-mediated knock-in. Lo CA, Greben AW, Chen BE. Biotechniques. 2017 Apr 1;62(4):165-174. doi: 10.2144/000114534. PubMed
Electric Cell-Substrate Impedance Sensing To Monitor Viral Growth and Study Cellular Responses to Infection with Alphaherpesviruses in Real Time. Pennington MR, Van de Walle GR. mSphere. 2017 Apr 5;2(2). pii: e00039-17. doi: 10.1128/mSphere.00039-17. eCollection 2017 Mar-Apr. PubMed
A new inhibitor of the beta-arrestin/AP2 endocytic complex reveals interplay between GPCR internalization and signalling. Beautrait A, Paradis JS, Zimmerman B, Giubilaro J, Nikolajev L, Armando S, Kobayashi H, Yamani L, Namkung Y, Heydenreich FM, Khoury E, Audet M, Roux PP, Veprintsev DB, Laporte SA, Bouvier M. Nat Commun. 2017 Apr 18;8:15054. doi: 10.1038/ncomms15054. PubMed
Frameshift indels introduced by genome editing can lead to in-frame exon skipping. Lalonde S, Stone OA, Lessard S, Lavertu A, Desjardins J, Beaudoin M, Rivas M, Stainier DYR, Lettre G. PLoS One. 2017 Jun 1;12(6):e0178700. doi: 10.1371/journal.pone.0178700. eCollection 2017. PubMed
Histone demethylase KDM5A regulates the ZMYND8-NuRD chromatin remodeler to promote DNA repair. Gong F, Clouaire T, Aguirrebengoa M, Legube G, Miller KM. J Cell Biol. 2017 Jul 3;216(7):1959-1974. doi: 10.1083/jcb.201611135. Epub 2017 Jun 1. PubMed
CRISPR/Cas9-Mediated Correction of the FANCD1 Gene in Primary Patient Cells. Skvarova Kramarzova K, Osborn MJ, Webber BR, DeFeo AP, McElroy AN, Kim CJ, Tolar J. Int J Mol Sci. 2017 Jun 14;18(6). pii: E1269. doi: 10.3390/ijms18061269. PubMed
Regulated Erlin-dependent release of the B12 transmembrane J-protein promotes ER membrane penetration of a non-enveloped virus. Inoue T, Tsai B. PLoS Pathog. 2017 Jun 14;13(6):e1006439. doi: 10.1371/journal.ppat.1006439. eCollection 2017 Jun. PubMed
Sexually dimorphic distribution of Prokr2 neurons revealed by the Prokr2-Cre mouse model. Mohsen Z, Sim H, Garcia-Galiano D, Han X, Bellefontaine N, Saunders TL, Elias CF. Brain Struct Funct. 2017 Jun 14. doi: 10.1007/s00429-017-1456-5. PubMed
Optimizing the DNA Donor Template for Homology-Directed Repair of Double-Strand Breaks. Song F, Stieger K. Mol Ther Nucleic Acids. 2017 Jun 16;7:53-60. doi: 10.1016/j.omtn.2017.02.006. Epub 2017 Feb 28. PubMed
Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells. Hindriksen S, Bramer AJ, Truong MA, Vromans MJM, Post JB, Verlaan-Klink I, Snippert HJ, Lens SMA, Hadders MA. PLoS One. 2017 Jun 22;12(6):e0179514. doi: 10.1371/journal.pone.0179514. eCollection 2017. PubMed
Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids. Noda T, Oji A, Ikawa M. Methods Mol Biol. 2017;1630:67-80. doi: 10.1007/978-1-4939-7128-2_6. PubMed
TSPAN12 Is a Norrin Co-receptor that Amplifies Frizzled4 Ligand Selectivity and Signaling. Lai MB, Zhang C, Shi J, Johnson V, Khandan L, McVey J, Klymkowsky MW, Chen Z, Junge HJ. Cell Rep. 2017 Jun 27;19(13):2809-2822. doi: 10.1016/j.celrep.2017.06.004. PubMed

Associated Plasmids

Generation of Genomic Deletions (of Rig-I GENE) in Goat Primary Cell Culture Using CRISPR/CAS9 Method. Malpotra S, Vats A, Kumar S, Gautam D, De S. Anim Biotechnol. 2018 Apr 3;29(2):142-152. doi: 10.1080/10495398.2017.1331915. Epub 2017 Jun 29. PubMed
Norrin-induced Frizzled4 endocytosis and endo-lysosomal trafficking control retinal angiogenesis and barrier function. Zhang C, Lai MB, Khandan L, Lee LA, Chen Z, Junge HJ. Nat Commun. 2017 Jul 4;8:16050. doi: 10.1038/ncomms16050. PubMed
Physiological and pathophysiological control of synaptic GluN2B-NMDA receptors by the C-terminal domain of amyloid precursor protein. Pousinha PA, Mouska X, Raymond EF, Gwizdek C, Dhib G, Poupon G, Zaragosi LE, Giudici C, Bethus I, Pacary E, Willem M, Marie H. Elife. 2017 Jul 6;6. doi: 10.7554/eLife.25659. PubMed

Associated Plasmids

The Protein Encoded by the CCDC170 Breast Cancer Gene Functions to Organize the Golgi-Microtubule Network. Jiang P, Li Y, Poleshko A, Medvedeva V, Baulina N, Zhang Y, Zhou Y, Slater CM, Pellegrin T, Wasserman J, Lindy M, Efimov A, Daly M, Katz RA, Chen X. EBioMedicine. 2017 Jun 27. pii: S2352-3964(17)30259-1. doi: 10.1016/j.ebiom.2017.06.024. PubMed
Inactivation of Pol theta and C-NHEJ eliminates off-target integration of exogenous DNA. Zelensky AN, Schimmel J, Kool H, Kanaar R, Tijsterman M. Nat Commun. 2017 Jul 7;8(1):66. doi: 10.1038/s41467-017-00124-3. PubMed
Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Flack JE, Mieszczanek J, Novcic N, Bienz M. Mol Cell. 2017 Jul 20;67(2):181-193.e5. doi: 10.1016/j.molcel.2017.06.009. Epub 2017 Jul 6. PubMed
In vivo CRISPR screening identifies Ptpn2 as a cancer immunotherapy target. Manguso RT, Pope HW, Zimmer MD, Brown FD, Yates KB, Miller BC, Collins NB, Bi K, LaFleur MW, Juneja VR, Weiss SA, Lo J, Fisher DE, Miao D, Van Allen E, Root DE, Sharpe AH, Doench JG, Haining WN. Nature. 2017 Jul 27;547(7664):413-418. doi: 10.1038/nature23270. Epub 2017 Jul 19. PubMed
Knockdown of EPHA1 Using CRISPR/CAS9 Suppresses Aggressive Properties of Ovarian Cancer Cells. Cui Y, Wu BO, Flamini V, Evans BAJ, Zhou D, Jiang WG. Anticancer Res. 2017 Aug;37(8):4415-4424. doi: 10.21873/anticanres.11836. PubMed
A Becn1 mutation mediates hyperactive autophagic sequestration of amyloid oligomers and improved cognition in Alzheimer's disease. Rocchi A, Yamamoto S, Ting T, Fan Y, Sadleir K, Wang Y, Zhang W, Huang S, Levine B, Vassar R, He C. PLoS Genet. 2017 Aug 14;13(8):e1006962. doi: 10.1371/journal.pgen.1006962. eCollection 2017 Aug. PubMed

Associated Plasmids

miR-322 stabilizes MEK1 expression to inhibit RAF/MEK/ERK pathway activation in cartilage. Bluhm B, Ehlen HWA, Holzer T, Georgieva VS, Heilig J, Pitzler L, Etich J, Bortecen T, Frie C, Probst K, Niehoff A, Belluoccio D, Van den Bergen J, Brachvogel B. Development. 2017 Oct 1;144(19):3562-3577. doi: 10.1242/dev.148429. Epub 2017 Aug 29. PubMed
A subset of conserved mammalian long non-coding RNAs are fossils of ancestral protein-coding genes. Hezroni H, Ben-Tov Perry R, Meir Z, Housman G, Lubelsky Y, Ulitsky I. Genome Biol. 2017 Aug 30;18(1):162. doi: 10.1186/s13059-017-1293-0. PubMed
The calcium-binding protein ALG-2 regulates protein secretion and trafficking via interactions with MISSL and MAP1B proteins. Takahara T, Inoue K, Arai Y, Kuwata K, Shibata H, Maki M. J Biol Chem. 2017 Oct 13;292(41):17057-17072. doi: 10.1074/jbc.M117.800201. Epub 2017 Sep 1. PubMed
Drusen in patient-derived hiPSC-RPE models of macular dystrophies. Galloway CA, Dalvi S, Hung SSC, MacDonald LA, Latchney LR, Wong RCB, Guymer RH, Mackey DA, Williams DS, Chung MM, Gamm DM, Pebay A, Hewitt AW, Singh R. Proc Natl Acad Sci U S A. 2017 Sep 26;114(39):E8214-E8223. doi: 10.1073/pnas.1710430114. Epub 2017 Sep 6. PubMed
Scc2/Nipbl hops between chromosomal cohesin rings after loading. Rhodes J, Mazza D, Nasmyth K, Uphoff S. Elife. 2017 Sep 15;6. pii: 30000. doi: 10.7554/eLife.30000. PubMed
Cohesin Can Remain Associated with Chromosomes during DNA Replication. Rhodes JDP, Haarhuis JHI, Grimm JB, Rowland BD, Lavis LD, Nasmyth KA. Cell Rep. 2017 Sep 19;20(12):2749-2755. doi: 10.1016/j.celrep.2017.08.092. PubMed
Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells. Fredriksson-Lidman K, Van Itallie CM, Tietgens AJ, Anderson JM. PLoS One. 2017 Sep 29;12(9):e0185448. doi: 10.1371/journal.pone.0185448. eCollection 2017. PubMed
Multiplex Enhancer Interference Reveals Collaborative Control of Gene Regulation by Estrogen Receptor alpha-Bound Enhancers. Carleton JB, Berrett KC, Gertz J. Cell Syst. 2017 Oct 25;5(4):333-344.e5. doi: 10.1016/j.cels.2017.08.011. Epub 2017 Sep 27. PubMed

Associated Plasmids

miR-155 induces ROS generation through downregulation of antioxidation-related genes in mesenchymal stem cells. Onodera Y, Teramura T, Takehara T, Obora K, Mori T, Fukuda K. Aging Cell. 2017 Dec;16(6):1369-1380. doi: 10.1111/acel.12680. Epub 2017 Oct 2. PubMed
RADX interacts with single-stranded DNA to promote replication fork stability. Schubert L, Ho T, Hoffmann S, Haahr P, Guerillon C, Mailand N. EMBO Rep. 2017 Nov;18(11):1991-2003. doi: 10.15252/embr.201744877. Epub 2017 Oct 11. PubMed
Enhanced Stem Cell Differentiation and Immunopurification of Genome Engineered Human Retinal Ganglion Cells. Sluch VM, Chamling X, Liu MM, Berlinicke CA, Cheng J, Mitchell KL, Welsbie DS, Zack DJ. Stem Cells Transl Med. 2017 Nov;6(11):1972-1986. doi: 10.1002/sctm.17-0059. Epub 2017 Oct 10. PubMed
Copb2 is essential for embryogenesis and hypomorphic mutations cause human microcephaly. DiStasio A, Driver A, Sund K, Donlin M, Muraleedharan RM, Pooya S, Kline-Fath B, Kaufman KM, Prows CA, Schorry E, Dasgupta B, Stottmann RW. Hum Mol Genet. 2017 Dec 15;26(24):4836-4848. doi: 10.1093/hmg/ddx362. PubMed
Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux. Vaites LP, Paulo JA, Huttlin EL, Harper JW. Mol Cell Biol. 2017 Dec 13;38(1). pii: MCB.00392-17. doi: 10.1128/MCB.00392-17. Print 2018 Jan 1. PubMed
Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca(2+)-ATPases and Key Regulators of Ca(2+) Clearance. Schmidt N, Kollewe A, Constantin CE, Henrich S, Ritzau-Jost A, Bildl W, Saalbach A, Hallermann S, Kulik A, Fakler B, Schulte U. Neuron. 2017 Nov 15;96(4):827-838.e9. doi: 10.1016/j.neuron.2017.09.038. Epub 2017 Oct 19. PubMed
Translational co-regulation of a ligand and inhibitor by a conserved RNA element. Zaucker A, Nagorska A, Kumari P, Hecker N, Wang Y, Huang S, Cooper L, Sivashanmugam L, VijayKumar S, Brosens J, Gorodkin J, Sampath K. Nucleic Acids Res. 2018 Jan 9;46(1):104-119. doi: 10.1093/nar/gkx938. PubMed
Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics. Akaike T, Ida T, Wei FY, Nishida M, Kumagai Y, Alam MM, Ihara H, Sawa T, Matsunaga T, Kasamatsu S, Nishimura A, Morita M, Tomizawa K, Nishimura A, Watanabe S, Inaba K, Shima H, Tanuma N, Jung M, Fujii S, Watanabe Y, Ohmuraya M, Nagy P, Feelisch M, Fukuto JM, Motohashi H. Nat Commun. 2017 Oct 27;8(1):1177. doi: 10.1038/s41467-017-01311-y. PubMed
Regulation of angiotensin II actions by enhancers and super-enhancers in vascular smooth muscle cells. Das S, Senapati P, Chen Z, Reddy MA, Ganguly R, Lanting L, Mandi V, Bansal A, Leung A, Zhang S, Jia Y, Wu X, Schones DE, Natarajan R. Nat Commun. 2017 Nov 13;8(1):1467. doi: 10.1038/s41467-017-01629-7. PubMed
Towards personalised allele-specific CRISPR gene editing to treat autosomal dominant disorders. Christie KA, Courtney DG, DeDionisio LA, Shern CC, De Majumdar S, Mairs LC, Nesbit MA, Moore CBT. Sci Rep. 2017 Nov 23;7(1):16174. doi: 10.1038/s41598-017-16279-4. PubMed
Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. Adikusuma F, Pfitzner C, Thomas PQ. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. PubMed

Associated Plasmids

MARCKS-related protein regulates cytoskeletal organization at cell-cell and cell-substrate contacts in epithelial cells. Van Itallie CM, Tietgens AJ, Aponte A, Gucek M, Cartagena-Rivera AX, Chadwick RS, Anderson JM. J Cell Sci. 2018 Feb 2;131(3). pii: jcs.210237. doi: 10.1242/jcs.210237. PubMed
Cytoplasmic Cl(-) couples membrane remodeling to epithelial morphogenesis. He M, Ye W, Wang WJ, Sison ES, Jan YN, Jan LY. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):E11161-E11169. doi: 10.1073/pnas.1714448115. Epub 2017 Dec 11. PubMed
RIPK3 promotes adenovirus type 5 activity. Weigert M, Binks A, Dowson S, Leung EYL, Athineos D, Yu X, Mullin M, Walton JB, Orange C, Ennis D, Blyth K, Tait SWG, McNeish IA. Cell Death Dis. 2017 Dec 13;8(12):3206. doi: 10.1038/s41419-017-0110-8. PubMed
AKT1 distinctively suppresses MyD88-depenedent and TRIF-dependent Toll-like receptor signaling in a kinase activity-independent manner. Zenke K, Muroi M, Tanamoto KI. Cell Signal. 2018 Mar;43:32-39. doi: 10.1016/j.cellsig.2017.12.002. Epub 2017 Dec 11. PubMed
AICAR suppresses TNF-alpha-induced complement factor B in RPE cells. Chung EJ, Efstathiou NE, Konstantinou EK, Maidana DE, Miller JW, Young LH, Vavvas DG. Sci Rep. 2017 Dec 15;7(1):17651. doi: 10.1038/s41598-017-17744-w. PubMed
Inhibition of intracellular lipolysis promotes human cancer cell adaptation to hypoxia. Zhang X, Saarinen AM, Hitosugi T, Wang Z, Wang L, Ho TH, Liu J. Elife. 2017 Dec 19;6. pii: 31132. doi: 10.7554/eLife.31132. PubMed
Distinct SoxB1 networks are required for naive and primed pluripotency. Corsinotti A, Wong FC, Tatar T, Szczerbinska I, Halbritter F, Colby D, Gogolok S, Pantier R, Liggat K, Mirfazeli ES, Hall-Ponsele E, Mullin NP, Wilson V, Chambers I. Elife. 2017 Dec 19;6. pii: 27746. doi: 10.7554/eLife.27746. PubMed
A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system. Tang N, Zhang Y, Pedrera M, Chang P, Baigent S, Moffat K, Shen Z, Nair V, Yao Y. Vaccine. 2018 Jan 29;36(5):716-722. doi: 10.1016/j.vaccine.2017.12.025. Epub 2018 Jan 2. PubMed
An engineered cell line lacking OGG1 and MUTYH glycosylases implicates the accumulation of genomic 8-oxoguanine as the basis for paraquat mutagenicity. Tajai P, Fedeles BI, Suriyo T, Navasumrit P, Kanitwithayanun J, Essigmann JM, Satayavivad J. Free Radic Biol Med. 2018 Feb 20;116:64-72. doi: 10.1016/j.freeradbiomed.2017.12.035. Epub 2017 Dec 28. PubMed
CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis. Smith MD, Harley ME, Kemp AJ, Wills J, Lee M, Arends M, von Kriegsheim A, Behrends C, Wilkinson S. Dev Cell. 2018 Jan 22;44(2):217-232.e11. doi: 10.1016/j.devcel.2017.11.024. Epub 2017 Dec 28. PubMed
Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection. Steyer B, Bu Q, Cory E, Jiang K, Duong S, Sinha D, Steltzer S, Gamm D, Chang Q, Saha K. Stem Cell Reports. 2018 Feb 13;10(2):642-654. doi: 10.1016/j.stemcr.2017.12.004. Epub 2018 Jan 4. PubMed
LINE-1 protein localization and functional dynamics during the cell cycle. Mita P, Wudzinska A, Sun X, Andrade J, Nayak S, Kahler DJ, Badri S, LaCava J, Ueberheide B, Yun CY, Fenyo D, Boeke JD. Elife. 2018 Jan 8;7. pii: 30058. doi: 10.7554/eLife.30058. PubMed
Heme oxygenase-1 affects generation and spontaneous cardiac differentiation of induced pluripotent stem cells. Stepniewski J, Pacholczak T, Skrzypczyk A, Ciesla M, Szade A, Szade K, Bidanel R, Langrzyk A, Grochowski R, Vandermeeren F, Kachamakova-Trojanowska N, Jez M, Drabik G, Nakanishi M, Jozkowicz A, Dulak J. IUBMB Life. 2018 Feb;70(2):129-142. doi: 10.1002/iub.1711. Epub 2018 Jan 9. PubMed
The WD40 domain of ATG16L1 is required for its non-canonical role in lipidation of LC3 at single membranes. Fletcher K, Ulferts R, Jacquin E, Veith T, Gammoh N, Arasteh JM, Mayer U, Carding SR, Wileman T, Beale R, Florey O. EMBO J. 2018 Feb 15;37(4). pii: embj.201797840. doi: 10.15252/embj.201797840. Epub 2018 Jan 9. PubMed
Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration. Kosmidou C, Efstathiou NE, Hoang MV, Notomi S, Konstantinou EK, Hirano M, Takahashi K, Maidana DE, Tsoka P, Young L, Gragoudas ES, Olsen TW, Morizane Y, Miller JW, Vavvas DG. Sci Rep. 2018 Jan 11;8(1):461. doi: 10.1038/s41598-017-17634-1. PubMed
Targeting mutant KRAS with CRISPR-Cas9 controls tumor growth. Kim W, Lee S, Kim HS, Song M, Cha YH, Kim YH, Shin J, Lee ES, Joo Y, Song JJ, Choi EJ, Choi JW, Lee J, Kang M, Yook JI, Lee MG, Kim YS, Paik S, Kim HH. Genome Res. 2018 Jan 11. pii: gr.223891.117. doi: 10.1101/gr.223891.117. PubMed
Role of miR-203 in estrogen receptor-mediated signaling in the rat uterus and endometrial carcinoma. Zierau O, Helle J, Schadyew S, Morgenroth Y, Bentler M, Hennig A, Chittur S, Tenniswood M, Kretzschmar G. J Cell Biochem. 2018 Jan 13. doi: 10.1002/jcb.26675. PubMed
Host expression of PD-L1 determines efficacy of PD-L1 pathway blockade-mediated tumor regression. Lin H, Wei S, Hurt EM, Green MD, Zhao L, Vatan L, Szeliga W, Herbst R, Harms PW, Fecher LA, Vats P, Chinnaiyan AM, Lao CD, Lawrence TS, Wicha M, Hamanishi J, Mandai M, Kryczek I, Zou W. J Clin Invest. 2018 Feb 1;128(2):805-815. doi: 10.1172/JCI96113. Epub 2018 Jan 16. PubMed
CRISPR/Cas9-Mediated Fluorescent Tagging of Endogenous Proteins in Human Pluripotent Stem Cells. Sharma A, Toepfer CN, Ward T, Wasson L, Agarwal R, Conner DA, Hu JH, Seidman CE. Curr Protoc Hum Genet. 2018 Jan 24;96:21.11.1-21.11.20. doi: 10.1002/cphg.52. PubMed
Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR/Cas9. Abbasi F, Miyata H, Ikawa M. Reprod Med Biol. 2017 Oct 14;17(1):3-10. doi: 10.1002/rmb2.12067. eCollection 2018 Jan. PubMed
Editing of the human TRIM5 gene to introduce mutations with the potential to inhibit HIV-1. Dufour C, Claudel A, Joubarne N, Merindol N, Maisonnet T, Masroori N, Plourde MB, Berthoux L. PLoS One. 2018 Jan 26;13(1):e0191709. doi: 10.1371/journal.pone.0191709. eCollection 2018. PubMed
ATR-Chk1 activation mitigates replication stress caused by mismatch repair-dependent processing of DNA damage. Gupta D, Lin B, Cowan A, Heinen CD. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):1523-1528. doi: 10.1073/pnas.1720355115. Epub 2018 Jan 29. PubMed
High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies. Youn JY, Dunham WH, Hong SJ, Knight JDR, Bashkurov M, Chen GI, Bagci H, Rathod B, MacLeod G, Eng SWM, Angers S, Morris Q, Fabian M, Cote JF, Gingras AC. Mol Cell. 2018 Feb 1;69(3):517-532.e11. doi: 10.1016/j.molcel.2017.12.020. Epub 2018 Jan 25. PubMed
miR-195 targets cyclin D3 and survivin to modulate the tumorigenesis of non-small cell lung cancer. Yu X, Zhang Y, Cavazos D, Ma X, Zhao Z, Du L, Pertsemlidis A. Cell Death Dis. 2018 Feb 7;9(2):193. doi: 10.1038/s41419-017-0219-9. PubMed
Reactivating TP53 signaling by the novel MDM2 inhibitor DS-3032b as a therapeutic option for high-risk neuroblastoma. Arnhold V, Schmelz K, Proba J, Winkler A, Wunschel J, Toedling J, Deubzer HE, Kunkele A, Eggert A, Schulte JH, Hundsdoerfer P. Oncotarget. 2017 Dec 18;9(2):2304-2319. doi: 10.18632/oncotarget.23409. eCollection 2018 Jan 5. PubMed
IRES-mediated translation of cofilin regulates axonal growth cone extension and turning. Choi JH, Wang W, Park D, Kim SH, Kim KT, Min KT. EMBO J. 2018 Mar 1;37(5). pii: embj.201695266. doi: 10.15252/embj.201695266. Epub 2018 Feb 12. PubMed
Comparative analysis of lipid-mediated CRISPR-Cas9 genome editing techniques. Ringer KP, Roth MG, Garey MS, Piorczynski TB, Suli A, Hansen JM, Alder JK. Cell Biol Int. 2018 Jul;42(7):849-858. doi: 10.1002/cbin.10952. Epub 2018 Mar 14. PubMed
Single-Molecule Dynamics and Localization of DNA Repair Proteins in Cells. Paul MW, Zelensky AN, Wyman C, Kanaar R. Methods Enzymol. 2018;600:375-406. doi: 10.1016/bs.mie.2017.11.015. Epub 2018 Feb 1. PubMed
Pharmacological targeting of MYC-regulated IRE1/XBP1 pathway suppresses MYC-driven breast cancer. Zhao N, Cao J, Xu L, Tang Q, Dobrolecki LE, Lv X, Talukdar M, Lu Y, Wang X, Hu DZ, Shi Q, Xiang Y, Wang Y, Liu X, Bu W, Jiang Y, Li M, Gong Y, Sun Z, Ying H, Yuan B, Lin X, Feng XH, Hartig SM, Li F, Shen H, Chen Y, Han L, Zeng Q, Patterson JB, Kaipparettu BA, Putluri N, Sicheri F, Rosen JM, Lewis MT, Chen X. J Clin Invest. 2018 Apr 2;128(4):1283-1299. doi: 10.1172/JCI95873. Epub 2018 Feb 26. PubMed
Calnexin is necessary for T cell transmigration into the central nervous system. Jung J, Eggleton P, Robinson A, Wang J, Gutowski N, Holley J, Newcombe J, Dudek E, Paul AM, Zochodne D, Kraus A, Power C, Agellon LB, Michalak M. JCI Insight. 2018 Mar 8;3(5). pii: 98410. doi: 10.1172/jci.insight.98410. PubMed
Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability. Johnson B, VanBlargan LA, Xu W, White JP, Shan C, Shi PY, Zhang R, Adhikari J, Gross ML, Leung DW, Diamond MS, Amarasinghe GK. Immunity. 2018 Mar 20;48(3):487-499.e5. doi: 10.1016/j.immuni.2018.01.014. Epub 2018 Mar 8. PubMed
The metabolic capacity of lipid droplet localized acyl-CoA synthetase 3 is not sufficient to support local triglyceride synthesis independent of the endoplasmic reticulum in A431 cells. Poppelreuther M, Sander S, Minden F, Dietz MS, Exner T, Du C, Zhang I, Ehehalt F, Knuppel L, Domschke S, Badenhop A, Staudacher S, Ehehalt R, Stremmel W, Thiele C, Heilemann M, Fullekrug J. Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Jun;1863(6):614-624. doi: 10.1016/j.bbalip.2018.03.003. Epub 2018 Mar 9. PubMed
Discovery and Characterization of ZUFSP/ZUP1, a Distinct Deubiquitinase Class Important for Genome Stability. Kwasna D, Abdul Rehman SA, Natarajan J, Matthews S, Madden R, De Cesare V, Weidlich S, Virdee S, Ahel I, Gibbs-Seymour I, Kulathu Y. Mol Cell. 2018 Apr 5;70(1):150-164.e6. doi: 10.1016/j.molcel.2018.02.023. Epub 2018 Mar 22. PubMed
Tumor suppressor functions of DAXX through histone H3.3/H3K9me3 pathway in pancreatic NETs. Ueda H, Akiyama Y, Shimada S, Mogushi K, Serizawa M, Matsumura S, Mitsunori Y, Aihara A, Ban D, Ochiai T, Kudo A, Tanabe M, Tanaka S. Endocr Relat Cancer. 2018 Jun;25(6):619-631. doi: 10.1530/ERC-17-0328. Epub 2018 Mar 29. PubMed
CRISPR/Cas9-mediated Genomic Editing of Cluap1/IFT38 Reveals a New Role in Actin Arrangement. Beyer T, Bolz S, Junger K, Horn N, Moniruzzaman M, Wissinger Y, Ueffing M, Boldt K. Mol Cell Proteomics. 2018 Jul;17(7):1285-1294. doi: 10.1074/mcp.RA117.000487. Epub 2018 Apr 3. PubMed
miR-195 potentiates the efficacy of microtubule-targeting agents in non-small cell lung cancer. Yu X, Zhang Y, Ma X, Pertsemlidis A. Cancer Lett. 2018 Jul 28;427:85-93. doi: 10.1016/j.canlet.2018.04.007. Epub 2018 Apr 13. PubMed
Activation of the NLRP3 Inflammasome Pathway by Uropathogenic Escherichia coli Is Virulence Factor-Dependent and Influences Colonization of Bladder Epithelial Cells. Demirel I, Persson A, Brauner A, Sarndahl E, Kruse R, Persson K. Front Cell Infect Microbiol. 2018 Mar 14;8:81. doi: 10.3389/fcimb.2018.00081. eCollection 2018. PubMed
Selection on the regulation of sympathetic nervous activity in humans and chimpanzees. Lee KS, Chatterjee P, Choi EY, Sung MK, Oh J, Won H, Park SM, Kim YJ, Yi SV, Choi JK. PLoS Genet. 2018 Apr 19;14(4):e1007311. doi: 10.1371/journal.pgen.1007311. eCollection 2018 Apr. PubMed
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair. Kato-Inui T, Takahashi G, Hsu S, Miyaoka Y. Nucleic Acids Res. 2018 May 18;46(9):4677-4688. doi: 10.1093/nar/gky264. PubMed
Enhanced Biosynthesis Performance of Heterologous Proteins in CHO-K1 Cells Using CRISPR-Cas9. Wang W, Zheng W, Hu F, He X, Wu D, Zhang W, Liu H, Ma X. ACS Synth Biol. 2018 May 18;7(5):1259-1268. doi: 10.1021/acssynbio.7b00375. Epub 2018 Apr 26. PubMed
Spatial and temporal organization of cadherin in punctate adherens junctions. Indra I, Choi J, Chen CS, Troyanovsky RB, Shapiro L, Honig B, Troyanovsky SM. Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4406-E4415. doi: 10.1073/pnas.1720826115. Epub 2018 Apr 24. PubMed
RhoA, Rac1, and Cdc42 differentially regulate alphaSMA and collagen I expression in mesenchymal stem cells. Ge J, Burnier L, Adamopoulou M, Kwa MQ, Schaks M, Rottner K, Brakebusch C. J Biol Chem. 2018 Jun 15;293(24):9358-9369. doi: 10.1074/jbc.RA117.001113. Epub 2018 Apr 26. PubMed
Mental Illnesses-Associated Fxr1 and Its Negative Regulator Gsk3beta Are Modulators of Anxiety and Glutamatergic Neurotransmission. Khlghatyan J, Evstratova A, Chamberland S, Marakhovskaia A, Bahremand A, Toth K, Beaulieu JM. Front Mol Neurosci. 2018 Apr 12;11:119. doi: 10.3389/fnmol.2018.00119. eCollection 2018. PubMed

Associated Plasmids

RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination. Ribeiro de Almeida C, Dhir S, Dhir A, Moghaddam AE, Sattentau Q, Meinhart A, Proudfoot NJ. Mol Cell. 2018 May 17;70(4):650-662.e8. doi: 10.1016/j.molcel.2018.04.001. Epub 2018 May 3. PubMed
Actin-dependent regulation of cilia length by the inverted formin FHDC1. Copeland SJ, McRae A, Guarguaglini G, Trinkle-Mulcahy L, Copeland JW. Mol Biol Cell. 2018 Jul 1;29(13):1611-1627. doi: 10.1091/mbc.E18-02-0088. Epub 2018 May 9. PubMed
qSR: a quantitative super-resolution analysis tool reveals the cell-cycle dependent organization of RNA Polymerase I in live human cells. Andrews JO, Conway W, Cho W-, Narayanan A, Spille J-, Jayanth N, Inoue T, Mullen S, Thaler J, Cisse II. Sci Rep. 2018 May 9;8(1):7424. doi: 10.1038/s41598-018-25454-0. PubMed
PRDX1 and MTH1 cooperate to prevent ROS-mediated inhibition of telomerase. Ahmed W, Lingner J. Genes Dev. 2018 May 1;32(9-10):658-669. doi: 10.1101/gad.313460.118. Epub 2018 May 17. PubMed
Hyaluronan negatively regulates vascular calcification involving BMP2 signaling. Kong Y, Liang Q, Chen Y, Yang P, Liu X, Li Y, Feng S, Wu J, Liu W, Tang J, Yu H, Ou JS, Lu L, Yan J. Lab Invest. 2018 Oct;98(10):1320-1332. doi: 10.1038/s41374-018-0076-x. Epub 2018 May 21. PubMed
High-throughput screening of prostate cancer risk loci by single nucleotide polymorphisms sequencing. Zhang P, Xia JH, Zhu J, Gao P, Tian YJ, Du M, Guo YC, Suleman S, Zhang Q, Kohli M, Tillmans LS, Thibodeau SN, French AJ, Cerhan JR, Wang LD, Wei GH, Wang L. Nat Commun. 2018 May 22;9(1):2022. doi: 10.1038/s41467-018-04451-x. PubMed
Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells. Lambrus BG, Moyer TC, Holland AJ. Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. PubMed
Aerobic Glycolysis Controls Myeloid-Derived Suppressor Cells and Tumor Immunity via a Specific CEBPB Isoform in Triple-Negative Breast Cancer. Li W, Tanikawa T, Kryczek I, Xia H, Li G, Wu K, Wei S, Zhao L, Vatan L, Wen B, Shu P, Sun D, Kleer C, Wicha M, Sabel M, Tao K, Wang G, Zou W. Cell Metab. 2018 Jul 3;28(1):87-103.e6. doi: 10.1016/j.cmet.2018.04.022. Epub 2018 May 24. PubMed
FBXL19 recruits CDK-Mediator to CpG islands of developmental genes priming them for activation during lineage commitment. Dimitrova E, Kondo T, Feldmann A, Nakayama M, Koseki Y, Konietzny R, Kessler BM, Koseki H, Klose RJ. Elife. 2018 May 29;7. pii: 37084. doi: 10.7554/eLife.37084. PubMed
Naturally Occurring Variants in LRP1 (Low-Density Lipoprotein Receptor-Related Protein 1) Affect HDL (High-Density Lipoprotein) Metabolism Through ABCA1 (ATP-Binding Cassette A1) and SR-B1 (Scavenger Receptor Class B Type 1) in Humans. Oldoni F, van Capelleveen JC, Dalila N, Wolters JC, Heeren J, Sinke RJ, Hui DY, Dallinga-Thie GM, Frikke-Schmidt R, Hovingh KG, van de Sluis B, Tybjaerg-Hansen A, Kuivenhoven JA. Arterioscler Thromb Vasc Biol. 2018 Jul;38(7):1440-1453. doi: 10.1161/ATVBAHA.117.310309. Epub 2018 May 31. PubMed
Development of a Reporter System to Explore MMEJ in the Context of Replacing Large Genomic Fragments. Yanik M, Ponnam SPG, Wimmer T, Trimborn L, Muller C, Gambert I, Ginsberg J, Janise A, Domicke J, Wende W, Lorenz B, Stieger K. Mol Ther Nucleic Acids. 2018 Jun 1;11:407-415. doi: 10.1016/j.omtn.2018.03.010. Epub 2018 Mar 22. PubMed
Silver nanoparticle-induced hormesis of astroglioma cells: A Mu-2-related death-inducing protein-orchestrated modus operandi. Choi JH, Min WK, Gopal J, Lee YM, Muthu M, Chun S, Oh JW. Int J Biol Macromol. 2018 Oct 1;117:1147-1156. doi: 10.1016/j.ijbiomac.2018.05.234. Epub 2018 Jun 2. PubMed
IRF1 supports DNA binding of STAT1 by promoting its phosphorylation. Zenke K, Muroi M, Tanamoto KI. Immunol Cell Biol. 2018 Nov;96(10):1095-1103. doi: 10.1111/imcb.12185. Epub 2018 Jun 28. PubMed
Quantitative in vivo whole genome motility screen reveals novel therapeutic targets to block cancer metastasis. Stoletov K, Willetts L, Paproski RJ, Bond DJ, Raha S, Jovel J, Adam B, Robertson AE, Wong F, Woolner E, Sosnowski DL, Bismar TA, Wong GK, Zijlstra A, Lewis JD. Nat Commun. 2018 Jun 14;9(1):2343. doi: 10.1038/s41467-018-04743-2. PubMed
Pathogenic variant in EPHB4 results in central conducting lymphatic anomaly. Li D, Wenger TL, Seiler C, March ME, Gutierrez-Uzquiza A, Kao C, Bhoj E, Tian L, Rosenbach M, Liu Y, Robinson N, Behr M, Chiavacci R, Hou C, Wang T, Bakay M, Pellegrino da Silva R, Perkins JA, Sleiman P, Levine MA, Hicks PJ, Itkin M, Dori Y, Hakonarson H. Hum Mol Genet. 2018 Sep 15;27(18):3233-3245. doi: 10.1093/hmg/ddy218. PubMed
NLRX1 resides in mitochondrial RNA granules and regulates mitochondrial RNA processing and bioenergetic adaptation. Singh K, Sripada L, Lipatova A, Roy M, Prajapati P, Gohel D, Bhatelia K, Chumakov PM, Singh R. Biochim Biophys Acta Mol Cell Res. 2018 Sep;1865(9):1260-1276. doi: 10.1016/j.bbamcr.2018.06.008. Epub 2018 Jun 20. PubMed
Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions. Murakami T, Kim J, Li Y, Green GE, Shikanov A, Ono A. Nat Commun. 2018 Jun 22;9(1):2436. doi: 10.1038/s41467-018-04846-w. PubMed
Lipin-1 regulates Bnip3-mediated mitophagy in glycolytic muscle. Alshudukhi AA, Zhu J, Huang D, Jama A, Smith JD, Wang QJ, Esser KA, Ren H. FASEB J. 2018 Jun 25:fj201800374. doi: 10.1096/fj.201800374. PubMed
A Novel CRISPR/Cas9-Based Cellular Model to Explore Adenylyl Cyclase and cAMP Signaling. Soto-Velasquez M, Hayes MP, Alpsoy A, Dykhuizen EC, Watts VJ. Mol Pharmacol. 2018 Sep;94(3):963-972. doi: 10.1124/mol.118.111849. Epub 2018 Jun 27. PubMed
Repurposing potential of 1st generation H1-specific antihistamines as anti-filovirus therapeutics. Schafer A, Cheng H, Xiong R, Soloveva V, Retterer C, Mo F, Bavari S, Thatcher G, Rong L. Antiviral Res. 2018 Sep;157:47-56. doi: 10.1016/j.antiviral.2018.07.003. Epub 2018 Jul 4. PubMed
gamma-Secretase promotes membrane insertion of the human papillomavirus L2 capsid protein during virus infection. Inoue T, Zhang P, Zhang W, Goodner-Bingham K, Dupzyk A, DiMaio D, Tsai B. J Cell Biol. 2018 Oct 1;217(10):3545-3559. doi: 10.1083/jcb.201804171. Epub 2018 Jul 13. PubMed
Starvation induces rapid degradation of selective autophagy receptors by endosomal microautophagy. Mejlvang J, Olsvik H, Svenning S, Bruun JA, Abudu YP, Larsen KB, Brech A, Hansen TE, Brenne H, Hansen T, Stenmark H, Johansen T. J Cell Biol. 2018 Oct 1;217(10):3640-3655. doi: 10.1083/jcb.201711002. Epub 2018 Jul 17. PubMed
Inhibition of Cyclin-Dependent Kinase 5: A Strategy to Improve Sorafenib Response in Hepatocellular Carcinoma Therapy. Ardelt MA, Frohlich T, Martini E, Muller M, Kanitz V, Atzberger C, Cantonati P, Messner M, Posselt L, Lehr T, Wojtyniak JG, Ulrich M, Arnold GJ, Konig L, Parazzoli D, Zahler S, Rothenfusser S, Mayr D, Gerbes A, Scita G, Vollmar AM, Pachmayr J. Hepatology. 2019 Jan;69(1):376-393. doi: 10.1002/hep.30190. Epub 2018 Dec 22. PubMed
DNA polymerase gamma (Polgamma) deficiency triggers a selective mTORC2 prosurvival autophagy response via mitochondria-mediated ROS signaling. Dhar SK, Bakthavatchalu V, Dhar B, Chen J, Tadahide I, Zhu H, Gao T, St Clair DK. Oncogene. 2018 Nov;37(48):6225-6242. doi: 10.1038/s41388-018-0404-z. Epub 2018 Jul 23. PubMed
CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells. Gupta N, Susa K, Yoda Y, Bonventre JV, Valerius MT, Morizane R. Curr Protoc Stem Cell Biol. 2018 May;45(1):e50. doi: 10.1002/cpsc.50. Epub 2018 Apr 26. PubMed
Tumor endothelial marker 8 promotes cancer progression and metastasis. Hoye AM, Tolstrup SD, Horton ER, Nicolau M, Frost H, Woo JH, Mauldin JP, Frankel AE, Cox TR, Erler JT. Oncotarget. 2018 Jul 10;9(53):30173-30188. doi: 10.18632/oncotarget.25734. eCollection 2018 Jul 10. PubMed
The actin filament bundling protein alpha-actinin-4 actually suppresses actin stress fibers by permitting actin turnover. Kemp JP Jr, Brieher WM. J Biol Chem. 2018 Sep 14;293(37):14520-14533. doi: 10.1074/jbc.RA118.004345. Epub 2018 Jul 26. PubMed
Probing the Function of Oncohistones Using Mutant Transgenes and Knock-In Mutations. Fang D, Wang H, Zhang Z. Methods Mol Biol. 2018;1832:339-356. doi: 10.1007/978-1-4939-8663-7_19. PubMed
Disruption by SaCas9 Endonuclease of HERV-Kenv, a Retroviral Gene with Oncogenic and Neuropathogenic Potential, Inhibits Molecules Involved in Cancer and Amyotrophic Lateral Sclerosis. Ibba G, Piu C, Uleri E, Serra C, Dolei A. Viruses. 2018 Aug 7;10(8). pii: v10080412. doi: 10.3390/v10080412. PubMed
Nuclear-resident RIG-I senses viral replication inducing antiviral immunity. Liu G, Lu Y, Thulasi Raman SN, Xu F, Wu Q, Li Z, Brownlie R, Liu Q, Zhou Y. Nat Commun. 2018 Aug 10;9(1):3199. doi: 10.1038/s41467-018-05745-w. PubMed
PARP2 mediates branched poly ADP-ribosylation in response to DNA damage. Chen Q, Kassab MA, Dantzer F, Yu X. Nat Commun. 2018 Aug 13;9(1):3233. doi: 10.1038/s41467-018-05588-5. PubMed
Distribution of Eg5 and TPX2 in mitosis: Insight from CRISPR tagged cells. Mann BJ, Wadsworth P. Cytoskeleton (Hoboken). 2018 Dec;75(12):508-521. doi: 10.1002/cm.21486. Epub 2018 Nov 15. PubMed
CRISPR/Cas9-Mediated Generation of Guangxi Bama Minipigs Harboring Three Mutations in alpha-Synuclein Causing Parkinson's Disease. Zhu XX, Zhong YZ, Ge YW, Lu KH, Lu SS. Sci Rep. 2018 Aug 20;8(1):12420. doi: 10.1038/s41598-018-30436-3. PubMed
RAP2 mediates mechanoresponses of the Hippo pathway. Meng Z, Qiu Y, Lin KC, Kumar A, Placone JK, Fang C, Wang KC, Lu S, Pan M, Hong AW, Moroishi T, Luo M, Plouffe SW, Diao Y, Ye Z, Park HW, Wang X, Yu FX, Chien S, Wang CY, Ren B, Engler AJ, Guan KL. Nature. 2018 Aug;560(7720):655-660. doi: 10.1038/s41586-018-0444-0. Epub 2018 Aug 22. PubMed
CALCOCO2/NDP52 and SQSTM1/p62 differentially regulate coxsackievirus B3 propagation. Mohamud Y, Qu J, Xue YC, Liu H, Deng H, Luo H. Cell Death Differ. 2018 Aug 28. pii: 10.1038/s41418-018-0185-5. doi: 10.1038/s41418-018-0185-5. PubMed
Inhibition of calpain-1 stabilizes TCF11/Nrf1 but does not affect its activation in response to proteasome inhibition. Nowak K, Taubert RM, Haberecht S, Venz S, Kruger E. Biosci Rep. 2018 Sep 19;38(5). pii: BSR20180393. doi: 10.1042/BSR20180393. Print 2018 Oct 31. PubMed
Facultative dosage compensation of developmental genes on autosomes in Drosophila and mouse embryonic stem cells. Valsecchi CIK, Basilicata MF, Semplicio G, Georgiev P, Gutierrez NM, Akhtar A. Nat Commun. 2018 Sep 7;9(1):3626. doi: 10.1038/s41467-018-05642-2. PubMed
Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration. Paul S, Dansithong W, Figueroa KP, Scoles DR, Pulst SM. Nat Commun. 2018 Sep 7;9(1):3648. doi: 10.1038/s41467-018-06041-3. PubMed
A CRISPR/Cas9 platform for MS2-labelling of single mRNA in live stem cells. Spille JH, Hecht M, Grube V, Cho WK, Lee C, Cisse II. Methods. 2019 Jan 15;153:35-45. doi: 10.1016/j.ymeth.2018.09.004. Epub 2018 Sep 12. PubMed
Sensitive and rapid quantification of exosomes by fusing luciferase to exosome marker proteins. Hikita T, Miyata M, Watanabe R, Oneyama C. Sci Rep. 2018 Sep 19;8(1):14035. doi: 10.1038/s41598-018-32535-7. PubMed
Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression. Parola C, Mason DM, Zingg A, Neumeier D, Reddy ST. Methods Mol Biol. 2018;1850:79-111. doi: 10.1007/978-1-4939-8730-6_7. PubMed
Loperamide, pimozide, and STF-62247 trigger autophagy-dependent cell death in glioblastoma cells. Zielke S, Meyer N, Mari M, Abou-El-Ardat K, Reggiori F, van Wijk SJL, Kogel D, Fulda S. Cell Death Dis. 2018 Sep 24;9(10):994. doi: 10.1038/s41419-018-1003-1. PubMed
The cargo receptor SURF4 promotes the efficient cellular secretion of PCSK9. Emmer BT, Hesketh GG, Kotnik E, Tang VT, Lascuna PJ, Xiang J, Gingras AC, Chen XW, Ginsburg D. Elife. 2018 Sep 25;7. pii: 38839. doi: 10.7554/eLife.38839. PubMed
Manifold roles of beta-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9. Luttrell LM, Wang J, Plouffe B, Smith JS, Yamani L, Kaur S, Jean-Charles PY, Gauthier C, Lee MH, Pani B, Kim J, Ahn S, Rajagopal S, Reiter E, Bouvier M, Shenoy SK, Laporte SA, Rockman HA, Lefkowitz RJ. Sci Signal. 2018 Sep 25;11(549). pii: 11/549/eaat7650. doi: 10.1126/scisignal.aat7650. PubMed
Inflammation-associated miR-155 activates differentiation of muscular satellite cells. Onodera Y, Teramura T, Takehara T, Itokazu M, Mori T, Fukuda K. PLoS One. 2018 Oct 1;13(10):e0204860. doi: 10.1371/journal.pone.0204860. eCollection 2018. PubMed
OTUB1 non-catalytically stabilizes the E2 ubiquitin-conjugating enzyme UBE2E1 by preventing its autoubiquitination. Pasupala N, Morrow ME, Que LT, Malynn BA, Ma A, Wolberger C. J Biol Chem. 2018 Nov 23;293(47):18285-18295. doi: 10.1074/jbc.RA118.004677. Epub 2018 Oct 3. PubMed

Associated Plasmids

Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis. Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp V. Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 10.1016/j.cels.2018.08.011. PubMed

Associated Plasmids

Human SEIPIN Binds Anionic Phospholipids. Yan R, Qian H, Lukmantara I, Gao M, Du X, Yan N, Yang H. Dev Cell. 2018 Oct 22;47(2):248-256.e4. doi: 10.1016/j.devcel.2018.09.010. Epub 2018 Oct 4. PubMed
CRISPR-mediated deletion of prostate cancer risk-associated CTCF loop anchors identifies repressive chromatin loops. Guo Y, Perez AA, Hazelett DJ, Coetzee GA, Rhie SK, Farnham PJ. Genome Biol. 2018 Oct 8;19(1):160. doi: 10.1186/s13059-018-1531-0. PubMed
A polymorphic residue that attenuates the antiviral potential of interferon lambda 4 in hominid lineages. Bamford CGG, Aranday-Cortes E, Filipe IC, Sukumar S, Mair D, Filipe ADS, Mendoza JL, Garcia KC, Fan S, Tishkoff SA, McLauchlan J. PLoS Pathog. 2018 Oct 11;14(10):e1007307. doi: 10.1371/journal.ppat.1007307. eCollection 2018 Oct. PubMed
Thyroid hormone signaling specifies cone subtypes in human retinal organoids. Eldred KC, Hadyniak SE, Hussey KA, Brenerman B, Zhang PW, Chamling X, Sluch VM, Welsbie DS, Hattar S, Taylor J, Wahlin K, Zack DJ, Johnston RJ Jr. Science. 2018 Oct 12;362(6411). pii: 362/6411/eaau6348. doi: 10.1126/science.aau6348. PubMed
High fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cells. Idoko-Akoh A, Taylor L, Sang HM, McGrew MJ. Sci Rep. 2018 Oct 11;8(1):15126. doi: 10.1038/s41598-018-33244-x. PubMed

Associated Plasmids

Probing aggrephagy using chemically-induced protein aggregates. Janssen AFJ, Katrukha EA, van Straaten W, Verlhac P, Reggiori F, Kapitein LC. Nat Commun. 2018 Oct 12;9(1):4245. doi: 10.1038/s41467-018-06674-4. PubMed

Associated Plasmids

Albatross/FBF1 contributes to both centriole duplication and centrosome separation. Inoko A, Yano T, Miyamoto T, Matsuura S, Kiyono T, Goshima N, Inagaki M, Hayashi Y. Genes Cells. 2018 Dec;23(12):1023-1042. doi: 10.1111/gtc.12648. Epub 2018 Nov 13. PubMed
Tracing the transitions from pluripotency to germ cell fate with CRISPR screening. Hackett JA, Huang Y, Gunesdogan U, Gretarsson KA, Kobayashi T, Surani MA. Nat Commun. 2018 Oct 16;9(1):4292. doi: 10.1038/s41467-018-06230-0. PubMed
A methyl-sensitive element induces bidirectional transcription in TATA-less CpG island-associated promoters. Mahpour A, Scruggs BS, Smiraglia D, Ouchi T, Gelman IH. PLoS One. 2018 Oct 17;13(10):e0205608. doi: 10.1371/journal.pone.0205608. eCollection 2018. PubMed
The epilepsy-associated protein TBC1D24 is required for normal development, survival and vesicle trafficking in mammalian neurons. Finelli MJ, Aprile D, Castroflorio E, Jeans A, Moschetta M, Chessum L, Degiacomi MT, Grasegger J, Lupien-Meilleur A, Bassett A, Rossignol E, Campeau PM, Bowl MR, Benfenati F, Fassio A, Oliver PL. Hum Mol Genet. 2019 Feb 15;28(4):584-597. doi: 10.1093/hmg/ddy370. PubMed
Up-regulated miR-133a orchestrates epithelial-mesenchymal transition of airway epithelial cells. Chen L, He X, Xie Y, Huang Y, Wolff DW, Abel PW, Tu Y. Sci Rep. 2018 Oct 19;8(1):15543. doi: 10.1038/s41598-018-33913-x. PubMed
betaTrCP is Required for HIV-1 Vpu Modulation of CD4, GaLV Env, and BST-2/Tetherin. Song YE, Cyburt D, Lucas TM, Gregory DA, Lyddon TD, Johnson MC. Viruses. 2018 Oct 19;10(10). pii: v10100573. doi: 10.3390/v10100573. PubMed
EHD2 is a mechanotransducer connecting caveolae dynamics with gene transcription. Torrino S, Shen WW, Blouin CM, Mani SK, Viaris de Lesegno C, Bost P, Grassart A, Koster D, Valades-Cruz CA, Chambon V, Johannes L, Pierobon P, Soumelis V, Coirault C, Vassilopoulos S, Lamaze C. J Cell Biol. 2018 Dec 3;217(12):4092-4105. doi: 10.1083/jcb.201801122. Epub 2018 Oct 22. PubMed
The Development and Use of Scalable Systems for Studying Aberrant Splicing in SF3B1-Mutant CLL. Murthy T, Paul KV, Minella AC, Pillai MM. Methods Mol Biol. 2019;1881:83-99. doi: 10.1007/978-1-4939-8876-1_7. PubMed
Analysis of the relationship between the KRAS G12V oncogene and the Hippo effector YAP1 in embryonal rhabdomyosarcoma. Mohamed AD, Shah N, Hettmer S, Vargesson N, Wackerhage H. Sci Rep. 2018 Oct 23;8(1):15674. doi: 10.1038/s41598-018-33852-7. PubMed
Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD. Chen J, Wang C, Kuang S. Methods Mol Biol. 2019;1889:25-41. doi: 10.1007/978-1-4939-8897-6_3. PubMed
Reciprocal inhibition of YAP/TAZ and NF-kappaB regulates osteoarthritic cartilage degradation. Deng Y, Lu J, Li W, Wu A, Zhang X, Tong W, Ho KK, Qin L, Song H, Mak KK. Nat Commun. 2018 Nov 1;9(1):4564. doi: 10.1038/s41467-018-07022-2. PubMed
p53-dependent autophagic degradation of TET2 modulates cancer therapeutic resistance. Zhang J, Tan P, Guo L, Gong J, Ma J, Li J, Lee M, Fang S, Jing J, Johnson G, Sun D, Cao WM, Dashwood R, Han L, Zhou Y, Dong WG, Huang Y. Oncogene. 2019 Mar;38(11):1905-1919. doi: 10.1038/s41388-018-0524-5. Epub 2018 Nov 2. PubMed
Genomic sequencing and editing revealed the GRM8 signaling pathway as potential therapeutic targets of squamous cell lung cancer. Zhang P, Kang B, Xie G, Li S, Gu Y, Shen Y, Zhao X, Ma Y, Li F, Si J, Wang J, Chen J, Yang H, Xu X, Yang Y. Cancer Lett. 2019 Feb 1;442:53-67. doi: 10.1016/j.canlet.2018.10.035. Epub 2018 Oct 27. PubMed
Critical Role of the UBL Domain in Stimulating the E3 Ubiquitin Ligase Activity of UHRF1 toward Chromatin. Foster BM, Stolz P, Mulholland CB, Montoya A, Kramer H, Bultmann S, Bartke T. Mol Cell. 2018 Nov 15;72(4):739-752.e9. doi: 10.1016/j.molcel.2018.09.028. Epub 2018 Nov 1. PubMed
Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Deng Y, Pakdel M, Blank B, Sundberg EL, Burd CG, von Blume J. Dev Cell. 2018 Nov 19;47(4):464-478.e8. doi: 10.1016/j.devcel.2018.10.012. Epub 2018 Nov 1. PubMed
A Screen Using iPSC-Derived Hepatocytes Reveals NAD(+) as a Potential Treatment for mtDNA Depletion Syndrome. Jing R, Corbett JL, Cai J, Beeson GC, Beeson CC, Chan SS, Dimmock DP, Lazcares L, Geurts AM, Lemasters JJ, Duncan SA. Cell Rep. 2018 Nov 6;25(6):1469-1484.e5. doi: 10.1016/j.celrep.2018.10.036. PubMed
Examining the role of the surfactant family member SFTA3 in interneuron specification. Chen CY, Anderson NC, Becker S, Schicht M, Stoddard C, Brauer L, Paulsen F, Grabel L. PLoS One. 2018 Nov 8;13(11):e0198703. doi: 10.1371/journal.pone.0198703. eCollection 2018. PubMed
Dynamic nanoscale morphology of the ER surveyed by STED microscopy. Schroeder LK, Barentine AES, Merta H, Schweighofer S, Zhang Y, Baddeley D, Bewersdorf J, Bahmanyar S. J Cell Biol. 2019 Jan 7;218(1):83-96. doi: 10.1083/jcb.201809107. Epub 2018 Nov 15. PubMed
Loss of the Chr16p11.2 ASD candidate gene QPRT leads to aberrant neuronal differentiation in the SH-SY5Y neuronal cell model. Haslinger D, Waltes R, Yousaf A, Lindlar S, Schneider I, Lim CK, Tsai MM, Garvalov BK, Acker-Palmer A, Krezdorn N, Rotter B, Acker T, Guillemin GJ, Fulda S, Freitag CM, Chiocchetti AG. Mol Autism. 2018 Nov 6;9:56. doi: 10.1186/s13229-018-0239-z. eCollection 2018. PubMed
Degenerate minigene library analysis enables identification of altered branch point utilization by mutant splicing factor 3B1 (SF3B1). Gupta AK, Murthy T, Paul KV, Ramirez O, Fisher JB, Rao S, Rosenberg AB, Seelig G, Minella AC, Pillai MM. Nucleic Acids Res. 2018 Nov 20. pii: 5193340. doi: 10.1093/nar/gky1161. PubMed

Associated Plasmids

hnRNP Q Regulates Internal Ribosome Entry Site-Mediated fmr1 Translation in Neurons. Choi JH, Kim SH, Jeong YH, Kim SW, Min KT, Kim KT. Mol Cell Biol. 2019 Feb 4;39(4). pii: MCB.00371-18. doi: 10.1128/MCB.00371-18. Print 2019 Feb 15. PubMed
Structural basis of RIP2 activation and signaling. Gong Q, Long Z, Zhong FL, Teo DET, Jin Y, Yin Z, Boo ZZ, Zhang Y, Zhang J, Yang R, Bhushan S, Reversade B, Li Z, Wu B. Nat Commun. 2018 Nov 26;9(1):4993. doi: 10.1038/s41467-018-07447-9. PubMed
The Tumor Suppressor SASH1 Interacts With the Signal Adaptor CRKL to Inhibit Epithelial-Mesenchymal Transition and Metastasis in Colorectal Cancer. Franke FC, Muller J, Abal M, Medina ED, Nitsche U, Weidmann H, Chardonnet S, Ninio E, Janssen KP. Cell Mol Gastroenterol Hepatol. 2018 Sep 11;7(1):33-53. doi: 10.1016/j.jcmgh.2018.08.007. eCollection 2019. PubMed
Lysophospholipases cooperate to mediate lipid homeostasis and lysophospholipid signaling. Wepy JA, Galligan JJ, Kingsley PJ, Xu S, Goodman MC, Tallman KA, Rouzer CA, Marnett LJ. J Lipid Res. 2019 Feb;60(2):360-374. doi: 10.1194/jlr.M087890. Epub 2018 Nov 27. PubMed
Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection. Sluch VM, Chamling X, Wenger C, Duan Y, Rice DS, Zack DJ. PLoS One. 2018 Nov 29;13(11):e0201683. doi: 10.1371/journal.pone.0201683. eCollection 2018. PubMed
Mitochondrial One-Carbon Pathway Supports Cytosolic Folate Integrity in Cancer Cells. Zheng Y, Lin TY, Lee G, Paddock MN, Momb J, Cheng Z, Li Q, Fei DL, Stein BD, Ramsamooj S, Zhang G, Blenis J, Cantley LC. Cell. 2018 Nov 29;175(6):1546-1560.e17. doi: 10.1016/j.cell.2018.09.041. PubMed
CD4+ T cells induce rejection of urothelial tumors after immune checkpoint blockade. Sato Y, Bolzenius JK, Eteleeb AM, Su X, Maher CA, Sehn JK, Arora VK. JCI Insight. 2018 Dec 6;3(23). pii: 121062. doi: 10.1172/jci.insight.121062. PubMed
RNA-seq Analysis of the SCN1A-KO Model based on CRISPR/Cas9 Genome Editing Technology. Shi X, He W, Guo S, Zhang B, Ren S, Liu K, Sun T, Cui J. Neuroscience. 2019 Feb 1;398:1-11. doi: 10.1016/j.neuroscience.2018.11.052. Epub 2018 Dec 8. PubMed
Heterochromatin delays CRISPR-Cas9 mutagenesis but does not influence the outcome of mutagenic DNA repair. Kallimasioti-Pazi EM, Thelakkad Chathoth K, Taylor GC, Meynert A, Ballinger T, Kelder MJE, Lalevee S, Sanli I, Feil R, Wood AJ. PLoS Biol. 2018 Dec 12;16(12):e2005595. doi: 10.1371/journal.pbio.2005595. eCollection 2018 Dec. PubMed
The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. Menzies SA, Volkmar N, van den Boomen DJ, Timms RT, Dickson AS, Nathan JA, Lehner PJ. Elife. 2018 Dec 13;7. pii: 40009. doi: 10.7554/eLife.40009. PubMed
Intracellular Iron Chelation by a Novel Compound, C7, Reactivates Epstein(-)Barr Virus (EBV) Lytic Cycle via the ERK-Autophagy Axis in EBV-Positive Epithelial Cancers. Yiu SPT, Hui KF, Choi CK, Kao RYT, Ma CW, Yang D, Chiang AKS. Cancers (Basel). 2018 Dec 11;10(12). pii: cancers10120505. doi: 10.3390/cancers10120505. PubMed
A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complex. Forrester A, De Leonibus C, Grumati P, Fasana E, Piemontese M, Staiano L, Fregno I, Raimondi A, Marazza A, Bruno G, Iavazzo M, Intartaglia D, Seczynska M, van Anken E, Conte I, De Matteis MA, Dikic I, Molinari M, Settembre C. EMBO J. 2019 Jan 15;38(2). pii: embj.201899847. doi: 10.15252/embj.201899847. Epub 2018 Dec 17. PubMed
Improved Delivery of CRISPR/Cas9 System Using Magnetic Nanoparticles into Porcine Fibroblast. Hryhorowicz M, Grzeskowiak B, Mazurkiewicz N, Sledzinski P, Lipinski D, Slomski R. Mol Biotechnol. 2019 Mar;61(3):173-180. doi: 10.1007/s12033-018-0145-9. PubMed
Hippo Pathway Regulation by Tyrosine Kinases. Reuven N, Shanzer M, Shaul Y. Methods Mol Biol. 2019;1893:215-236. doi: 10.1007/978-1-4939-8910-2_17. PubMed
Synthetic mammalian pattern formation driven by differential diffusivity of Nodal and Lefty. Sekine R, Shibata T, Ebisuya M. Nat Commun. 2018 Dec 21;9(1):5456. doi: 10.1038/s41467-018-07847-x. PubMed
CRISPR/Cas9-mediated knockout of the PDEF gene inhibits migration and invasion of human gastric cancer AGS cells. Zhang YQ, Pei JH, Shi SS, Guo XS, Cui GY, Li YF, Zhang HP, Hu WQ. Biomed Pharmacother. 2019 Mar;111:76-85. doi: 10.1016/j.biopha.2018.12.048. Epub 2018 Dec 19. PubMed
The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis. Volkmar N, Thezenas ML, Louie SM, Juszkiewicz S, Nomura DK, Hegde RS, Kessler BM, Christianson JC. J Cell Sci. 2019 Jan 16;132(2). pii: jcs.223453. doi: 10.1242/jcs.223453. PubMed
Disruption of sialic acid metabolism drives tumor growth by augmenting CD8(+) T cell apoptosis. Cornelissen LAM, Blanas A, van der Horst JC, Kruijssen L, Zaal A, O'Toole T, Wiercx L, van Kooyk Y, van Vliet SJ. Int J Cancer. 2019 May 1;144(9):2290-2302. doi: 10.1002/ijc.32084. Epub 2019 Jan 3. PubMed
TULP3 is required for localization of membrane-associated proteins ARL13B and INPP5E to primary cilia. Han S, Miyoshi K, Shikada S, Amano G, Wang Y, Yoshimura T, Katayama T. Biochem Biophys Res Commun. 2019 Jan 29;509(1):227-234. doi: 10.1016/j.bbrc.2018.12.109. Epub 2018 Dec 21. PubMed
The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation. Chen D, Liu W, Zimmerman J, Pastor WA, Kim R, Hosohama L, Ho J, Aslanyan M, Gell JJ, Jacobsen SE, Clark AT. Cell Rep. 2018 Dec 26;25(13):3591-3602.e5. doi: 10.1016/j.celrep.2018.12.011. PubMed
Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives chromatin association. Connelly KE, Weaver TM, Alpsoy A, Gu BX, Musselman CA, Dykhuizen EC. Nucleic Acids Res. 2018 Dec 29. pii: 5266947. doi: 10.1093/nar/gky1290. PubMed

Associated Plasmids

Dengue virus requires apoptosis linked gene-2-interacting protein X (ALIX) for viral propagation. Thepparit C, Khongwichit S, Ketsuwan K, Libsittikul S, Auewarakul P, Smith DR. Virus Res. 2019 Feb;261:65-71. doi: 10.1016/j.virusres.2018.12.015. Epub 2018 Dec 29. PubMed
CD46 Null Packaging Cell Line Improves Measles Lentiviral Vector Production and Gene Delivery to Hematopoietic Stem and Progenitor Cells. Ozog S, Chen CX, Simpson E, Garijo O, Timberlake ND, Minder P, Verhoeyen E, Torbett BE. Mol Ther Methods Clin Dev. 2018 Nov 24;13:27-39. doi: 10.1016/j.omtm.2018.11.006. eCollection 2019 Jun 14. PubMed
Rac-Mediated Macropinocytosis of Extracellular Protein Promotes Glucose Independence in Non-Small Cell Lung Cancer. Hodakoski C, Hopkins BD, Zhang G, Su T, Cheng Z, Morris R, Rhee KY, Goncalves MD, Cantley LC. Cancers (Basel). 2019 Jan 2;11(1). pii: cancers11010037. doi: 10.3390/cancers11010037. PubMed
The Gata2 repression during 3T3-L1 preadipocyte differentiation is dependent on a rapid decrease in histone acetylation in response to glucocorticoid receptor activation. Ishijima Y, Ohmori S, Uneme A, Aoki Y, Kobori M, Ohida T, Arai M, Hosaka M, Ohneda K. Mol Cell Endocrinol. 2019 Mar 1;483:39-49. doi: 10.1016/j.mce.2019.01.002. Epub 2019 Jan 4. PubMed
A cellular complex of BACE1 and gamma-secretase sequentially generates Abeta from its full-length precursor. Liu L, Ding L, Rovere M, Wolfe MS, Selkoe DJ. J Cell Biol. 2019 Feb 4;218(2):644-663. doi: 10.1083/jcb.201806205. Epub 2019 Jan 9. PubMed
BRCA2 deficiency instigates cGAS-mediated inflammatory signaling and confers sensitivity to tumor necrosis factor-alpha-mediated cytotoxicity. Heijink AM, Talens F, Jae LT, van Gijn SE, Fehrmann RSN, Brummelkamp TR, van Vugt MATM. Nat Commun. 2019 Jan 9;10(1):100. doi: 10.1038/s41467-018-07927-y. PubMed
The novel lncRNA lnc-NR2F1 is pro-neurogenic and mutated in human neurodevelopmental disorders. Ang CE, Ma Q, Wapinski OL, Fan S, Flynn RA, Lee QY, Coe B, Onoguchi M, Olmos VH, Do BT, Dukes-Rimsky L, Xu J, Tanabe K, Wang L, Elling U, Penninger JM, Zhao Y, Qu K, Eichler EE, Srivastava A, Wernig M, Chang HY. Elife. 2019 Jan 10;8. pii: 41770. doi: 10.7554/eLife.41770. PubMed
Chk1-mediated Cdc25A degradation as a critical mechanism for normal cell cycle progression. Goto H, Natsume T, Kanemaki MT, Kaito A, Wang S, Gabazza EC, Inagaki M, Mizoguchi A. J Cell Sci. 2019 Jan 25;132(2). pii: jcs.223123. doi: 10.1242/jcs.223123. PubMed
Human blood vessel organoids as a model of diabetic vasculopathy. Wimmer RA, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, Taubenschmid J, Hammerle M, Esk C, Bagley JA, Lindenhofer D, Chen G, Boehm M, Agu CA, Yang F, Fu B, Zuber J, Knoblich JA, Kerjaschki D, Penninger JM. Nature. 2019 Jan;565(7740):505-510. doi: 10.1038/s41586-018-0858-8. Epub 2019 Jan 16. PubMed
Skeletal muscle-specific Prmt1 deletion causes muscle atrophy via deregulation of the PRMT6-FOXO3 axis. Choi S, Jeong HJ, Kim H, Choi D, Cho SC, Seong JK, Koo SH, Kang JS. Autophagy. 2019 Jun;15(6):1069-1081. doi: 10.1080/15548627.2019.1569931. Epub 2019 Feb 5. PubMed
DNA polymerase theta (POLQ) is important for repair of DNA double-strand breaks caused by fork collapse. Wang Z, Song Y, Li S, Kurian S, Xiang R, Chiba T, Wu X. J Biol Chem. 2019 Mar 15;294(11):3909-3919. doi: 10.1074/jbc.RA118.005188. Epub 2019 Jan 17. PubMed
Desmoglein 2 mutation provokes skeletal muscle actin expression and accumulation at intercalated discs in murine hearts. Kant S, Freytag B, Herzog A, Reich A, Merkel R, Hoffmann B, Krusche CA, Leube RE. J Cell Sci. 2019 Feb 18;132(5). pii: jcs.199612. doi: 10.1242/jcs.199612. PubMed
The integral function of the endocytic recycling compartment is regulated by RFFL-mediated ubiquitylation of Rab11 effectors. Sakai R, Fukuda R, Unida S, Aki M, Ono Y, Endo A, Kusumi S, Koga D, Fukushima T, Komada M, Okiyoneda T. J Cell Sci. 2019 Feb 7;132(3). pii: jcs.228007. doi: 10.1242/jcs.228007. PubMed
Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line. Schmid B, Prehn KR, Nimsanor N, Garcia BIA, Poulsen U, Jorring I, Rasmussen MA, Clausen C, Mau-Holzmann UA, Ramakrishna S, Muddashetty R, Steeg R, Bruce K, Mackintosh P, Ebneth A, Holst B, Cabrera-Socorro A. Stem Cell Res. 2019 Jan;34:101349. doi: 10.1016/j.scr.2018.11.010. Epub 2019 Jan 4. PubMed
Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Agrotis A, Pengo N, Burden JJ, Ketteler R. Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925. PubMed

Associated Plasmids

Differential ACPA Binding to Nuclear Antigens Reveals a PAD-Independent Pathway and a Distinct Subset of Acetylation Cross-Reactive Autoantibodies in Rheumatoid Arthritis. Lloyd KA, Wigerblad G, Sahlstrom P, Garimella MG, Chemin K, Steen J, Titcombe PJ, Marklein B, Zhou D, Stalesen R, Ossipova E, Lundqvist C, Ekwall O, Ronnelid J, Mueller DL, Karlsson MCI, Kaplan MJ, Skriner K, Klareskog L, Wermeling F, Malmstrom V, Gronwall C. Front Immunol. 2019 Jan 4;9:3033. doi: 10.3389/fimmu.2018.03033. eCollection 2018. PubMed
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing. Tang N, Zhang Y, Pedrera M, Chang P, Baigent S, Moffat K, Shen Z, Nair V, Yao Y. J Vis Exp. 2019 Jan 7;(143). doi: 10.3791/58193. PubMed
Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies. Haidar M, Asselbergh B, Adriaenssens E, De Winter V, Timmermans JP, Auer-Grumbach M, Juneja M, Timmerman V. Autophagy. 2019 Jun;15(6):1051-1068. doi: 10.1080/15548627.2019.1569930. Epub 2019 Jan 31. PubMed
The circadian clock components BMAL1 and REV-ERBalpha regulate flavivirus replication. Zhuang X, Magri A, Hill M, Lai AG, Kumar A, Rambhatla SB, Donald CL, Lopez-Clavijo AF, Rudge S, Pinnick K, Chang WH, Wing PAC, Brown R, Qin X, Simmonds P, Baumert TF, Ray D, Loudon A, Balfe P, Wakelam M, Butterworth S, Kohl A, Jopling CL, Zitzmann N, McKeating JA. Nat Commun. 2019 Jan 22;10(1):377. doi: 10.1038/s41467-019-08299-7. PubMed
PKG1-modified TSC2 regulates mTORC1 activity to counter adverse cardiac stress. Ranek MJ, Kokkonen-Simon KM, Chen A, Dunkerly-Eyring BL, Vera MP, Oeing CU, Patel CH, Nakamura T, Zhu G, Bedja D, Sasaki M, Holewinski RJ, Van Eyk JE, Powell JD, Lee DI, Kass DA. Nature. 2019 Feb;566(7743):264-269. doi: 10.1038/s41586-019-0895-y. Epub 2019 Jan 30. PubMed
The AAA+ ATPase/ubiquitin ligase mysterin stabilizes cytoplasmic lipid droplets. Sugihara M, Morito D, Ainuki S, Hirano Y, Ogino K, Kitamura A, Hirata H, Nagata K. J Cell Biol. 2019 Mar 4;218(3):949-960. doi: 10.1083/jcb.201712120. Epub 2019 Jan 31. PubMed
Single-cell quantification of the concentrations and dissociation constants of endogenous proteins. Komatsubara AT, Goto Y, Kondo Y, Matsuda M, Aoki K. J Biol Chem. 2019 Apr 12;294(15):6062-6072. doi: 10.1074/jbc.RA119.007685. Epub 2019 Feb 9. PubMed

Associated Plasmids

Mitochondrial protein E2F3d, a distinctive E2F3 product, mediates hypoxia-induced mitophagy in cancer cells. Araki K, Kawauchi K, Sugimoto W, Tsuda D, Oda H, Yoshida R, Ohtani K. Commun Biol. 2019 Jan 3;2:3. doi: 10.1038/s42003-018-0246-9. eCollection 2019. PubMed
Demonstration of critical role of GRIN3A in nicotine dependence through both genetic association and molecular functional studies. Chen J, Liu Q, Fan R, Han H, Yang Z, Cui W, Song G, Li MD. Addict Biol. 2019 Feb 11. doi: 10.1111/adb.12718. PubMed
An alternative membrane topology permits lipid droplet localization of peroxisomal fatty acyl-CoA reductase 1. Exner T, Romero-Brey I, Yifrach E, Rivera-Monroy J, Schrul B, Zouboulis CC, Stremmel W, Honsho M, Bartenschlager R, Zalckvar E, Poppelreuther M, Fullekrug J. J Cell Sci. 2019 Mar 18;132(6). pii: jcs.223016. doi: 10.1242/jcs.223016. PubMed
Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast. Hayashi A, Tanaka K. G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 10.1534/g3.118.200976. PubMed

Associated Plasmids

A Y-chromosome shredding gene drive for controlling pest vertebrate populations. Prowse TA, Adikusuma F, Cassey P, Thomas P, Ross JV. Elife. 2019 Feb 15;8. pii: 41873. doi: 10.7554/eLife.41873. PubMed
Gene Editing of Mouse Embryonic and Epiblast Stem Cells. Sibbritt T, Osteil P, Fan X, Sun J, Salehin N, Knowles H, Shen J, Tam PPL. Methods Mol Biol. 2019;1940:77-95. doi: 10.1007/978-1-4939-9086-3_6. PubMed
Defining the Functional Role of NaV1.7 in Human Nociception. McDermott LA, Weir GA, Themistocleous AC, Segerdahl AR, Blesneac I, Baskozos G, Clark AJ, Millar V, Peck LJ, Ebner D, Tracey I, Serra J, Bennett DL. Neuron. 2019 Mar 6;101(5):905-919.e8. doi: 10.1016/j.neuron.2019.01.047. Epub 2019 Feb 19. PubMed
CRISPR/Cas9-Assisted Genome Editing in Murine Embryonic Stem Cells. Gruzdev A, Scott GJ, Hagler TB, Ray MK. Methods Mol Biol. 2019;1960:1-21. doi: 10.1007/978-1-4939-9167-9_1. PubMed
System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection. Garcia-Moreno M, Noerenberg M, Ni S, Jarvelin AI, Gonzalez-Almela E, Lenz CE, Bach-Pages M, Cox V, Avolio R, Davis T, Hester S, Sohier TJM, Li B, Heikel G, Michlewski G, Sanz MA, Carrasco L, Ricci EP, Pelechano V, Davis I, Fischer B, Mohammed S, Castello A. Mol Cell. 2019 Apr 4;74(1):196-211.e11. doi: 10.1016/j.molcel.2019.01.017. Epub 2019 Feb 21. PubMed
Depletion of the RNA binding protein HNRNPD impairs homologous recombination by inhibiting DNA-end resection and inducing R-loop accumulation. Alfano L, Caporaso A, Altieri A, Dell'Aquila M, Landi C, Bini L, Pentimalli F, Giordano A. Nucleic Acids Res. 2019 May 7;47(8):4068-4085. doi: 10.1093/nar/gkz076. PubMed
SH3BP4 Regulates Intestinal Stem Cells and Tumorigenesis by Modulating beta-Catenin Nuclear Localization. Antas P, Novellasdemunt L, Kucharska A, Massie I, Carvalho J, Oukrif D, Nye E, Novelli M, Li VSW. Cell Rep. 2019 Feb 26;26(9):2266-2273.e4. doi: 10.1016/j.celrep.2019.01.110. PubMed
The HERV-K accessory protein Np9 controls viability and migration of teratocarcinoma cells. Chan SM, Sapir T, Park SS, Rual JF, Contreras-Galindo R, Reiner O, Markovitz DM. PLoS One. 2019 Feb 28;14(2):e0212970. doi: 10.1371/journal.pone.0212970. eCollection 2019. PubMed
Selective Roles of Vertebrate PCF11 in Premature and Full-Length Transcript Termination. Kamieniarz-Gdula K, Gdula MR, Panser K, Nojima T, Monks J, Wisniewski JR, Riepsaame J, Brockdorff N, Pauli A, Proudfoot NJ. Mol Cell. 2019 Apr 4;74(1):158-172.e9. doi: 10.1016/j.molcel.2019.01.027. Epub 2019 Feb 25. PubMed
Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X. Colognori D, Sunwoo H, Kriz AJ, Wang CY, Lee JT. Mol Cell. 2019 Apr 4;74(1):101-117.e10. doi: 10.1016/j.molcel.2019.01.015. Epub 2019 Feb 28. PubMed
Phosphorylation of Syntaxin 17 by TBK1 Controls Autophagy Initiation. Kumar S, Gu Y, Abudu YP, Bruun JA, Jain A, Farzam F, Mudd M, Anonsen JH, Rusten TE, Kasof G, Ktistakis N, Lidke KA, Johansen T, Deretic V. Dev Cell. 2019 Apr 8;49(1):130-144.e6. doi: 10.1016/j.devcel.2019.01.027. Epub 2019 Feb 28. PubMed
A Stromal Lysolipid-Autotaxin Signaling Axis Promotes Pancreatic Tumor Progression. Auciello FR, Bulusu V, Oon C, Tait-Mulder J, Berry M, Bhattacharyya S, Tumanov S, Allen-Petersen BL, Link J, Kendsersky ND, Vringer E, Schug M, Novo D, Hwang RF, Evans RM, Nixon C, Dorrell C, Morton JP, Norman JC, Sears RC, Kamphorst JJ, Sherman MH. Cancer Discov. 2019 May;9(5):617-627. doi: 10.1158/2159-8290.CD-18-1212. Epub 2019 Mar 5. PubMed
Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins. Zhu Y, Gao A, Zhan Q, Wang Y, Feng H, Liu S, Gao G, Serganov A, Gao P. Mol Cell. 2019 Apr 18;74(2):296-309.e7. doi: 10.1016/j.molcel.2019.01.038. Epub 2019 Mar 5. PubMed
Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy. Vargas JNS, Wang C, Bunker E, Hao L, Maric D, Schiavo G, Randow F, Youle RJ. Mol Cell. 2019 Apr 18;74(2):347-362.e6. doi: 10.1016/j.molcel.2019.02.010. Epub 2019 Mar 7. PubMed

Associated Plasmids

A methylation-phosphorylation switch determines Plk1 kinase activity and function in DNA damage repair. Li W, Wang HY, Zhao X, Duan H, Cheng B, Liu Y, Zhao M, Shu W, Mei Y, Wen Z, Tang M, Guo L, Li G, Chen Q, Liu X, Du HN. Sci Adv. 2019 Mar 6;5(3):eaau7566. doi: 10.1126/sciadv.aau7566. eCollection 2019 Mar. PubMed
Establishment of CRISPR/Cas9-Mediated Knock-in System for Porcine Cells with High Efficiency. Zhang J, Zhu Z, Yue W, Li J, Chen Q, Yan Y, Lei A, Hua J. Appl Biochem Biotechnol. 2019 Mar 12. pii: 10.1007/s12010-019-02984-5. doi: 10.1007/s12010-019-02984-5. PubMed
Mitophagy regulates mitochondrial network signaling, oxidative stress, and apoptosis during myoblast differentiation. Baechler BL, Bloemberg D, Quadrilatero J. Autophagy. 2019 Sep;15(9):1606-1619. doi: 10.1080/15548627.2019.1591672. Epub 2019 Apr 7. PubMed
ELMOD2 regulates mitochondrial fusion in a mitofusin-dependent manner, downstream of ARL2. Schiavon CR, Turn RE, Newman LE, Kahn RA. Mol Biol Cell. 2019 May 1;30(10):1198-1213. doi: 10.1091/mbc.E18-12-0804. Epub 2019 Mar 13. PubMed
Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements. Thormann V, Glaser LV, Rothkegel MC, Borschiwer M, Bothe M, Fuchs A, Meijsing SH. Life Sci Alliance. 2019 Mar 13;2(2). pii: 2/2/e201800283. doi: 10.26508/lsa.201800283. Print 2019 Apr. PubMed
Inadequate BiP availability defines endoplasmic reticulum stress. Vitale M, Bakunts A, Orsi A, Lari F, Tade L, Danieli A, Rato C, Valetti C, Sitia R, Raimondi A, Christianson JC, van Anken E. Elife. 2019 Mar 14;8. pii: 41168. doi: 10.7554/eLife.41168. PubMed
PMEPA1/TMEPAI knockout impairs tumour growth and lung metastasis in MDA-MB-231 cells without changing monolayer culture cell growth. Abdelaziz M, Watanabe Y, Kato M. J Biochem. 2019 May 1;165(5):411-414. doi: 10.1093/jb/mvz022. PubMed
Lysosomal targeting of the ABC transporter TAPL is determined by membrane-localized charged residues. Graab P, Bock C, Weiss K, Hirth A, Koller N, Braner M, Jung J, Loehr F, Tampe R, Behrends C, Abele R. J Biol Chem. 2019 May 3;294(18):7308-7323. doi: 10.1074/jbc.RA118.007071. Epub 2019 Mar 15. PubMed
LION: a simple and rapid method to achieve CRISPR gene editing. Xiang X, Luo L, Nodzynski M, Li C, Han P, Dou H, Petersen TS, Liang X, Pan X, Qu K, Yang L, Dang Y, Liu X, Bolund L, Zhang X, Tong G, Xing Y, Luo Y, Lin L. Cell Mol Life Sci. 2019 Jul;76(13):2633-2645. doi: 10.1007/s00018-019-03064-x. Epub 2019 Mar 18. PubMed
Negative Regulation of p53-Induced Senescence by N-WASP Is Crucial for DMBA/TPA-Induced Skin Tumor Formation. Li H, Petersen S, Garcia Mariscal A, Brakebusch C. Cancer Res. 2019 May 1;79(9):2167-2181. doi: 10.1158/0008-5472.CAN-18-1253. Epub 2019 Mar 20. PubMed
Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders. Sauter EJ, Kutsche LK, Klapper SD, Busskamp V. Methods Mol Biol. 2019;1942:101-121. doi: 10.1007/978-1-4939-9080-1_9. PubMed

Associated Plasmids

A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis. Sun S, Li F, Liu Y, Qu H, Wong SW, Zeng L, Yu M, Feng H, Liu H, Han D. Arch Oral Biol. 2019 May;101:100-107. doi: 10.1016/j.archoralbio.2019.03.013. Epub 2019 Mar 18. PubMed
Defined conditions for propagation and manipulation of mouse embryonic stem cells. Mulas C, Kalkan T, von Meyenn F, Leitch HG, Nichols J, Smith A. Development. 2019 Mar 26;146(6). pii: 146/6/dev173146. doi: 10.1242/dev.173146. PubMed
Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression. Knapp DJHF, Michaels YS, Jamilly M, Ferry QRV, Barbosa H, Milne TA, Fulga TA. Nat Commun. 2019 Apr 2;10(1):1490. doi: 10.1038/s41467-019-09148-3. PubMed
Newly synthesized claudins but not occludin are added to the basal side of the tight junction. Van Itallie CM, Lidman KF, Tietgens AJ, Anderson JM. Mol Biol Cell. 2019 Jun 1;30(12):1406-1424. doi: 10.1091/mbc.E19-01-0008. Epub 2019 Apr 3. PubMed
Genetic compensation triggered by mutant mRNA degradation. El-Brolosy MA, Kontarakis Z, Rossi A, Kuenne C, Gunther S, Fukuda N, Kikhi K, Boezio GLM, Takacs CM, Lai SL, Fukuda R, Gerri C, Giraldez AJ, Stainier DYR. Nature. 2019 Apr;568(7751):193-197. doi: 10.1038/s41586-019-1064-z. Epub 2019 Apr 3. PubMed
Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory. Bertero A, Fields PA, Ramani V, Bonora G, Yardimci GG, Reinecke H, Pabon L, Noble WS, Shendure J, Murry CE. Nat Commun. 2019 Apr 4;10(1):1538. doi: 10.1038/s41467-019-09483-5. PubMed
ATG2 transports lipids to promote autophagosome biogenesis. Valverde DP, Yu S, Boggavarapu V, Kumar N, Lees JA, Walz T, Reinisch KM, Melia TJ. J Cell Biol. 2019 Jun 3;218(6):1787-1798. doi: 10.1083/jcb.201811139. Epub 2019 Apr 5. PubMed
Complex Formation between VEGFR2 and the beta2-Adrenoceptor. Kilpatrick LE, Alcobia DC, White CW, Peach CJ, Glenn JR, Zimmerman K, Kondrashov A, Pfleger KDG, Ohana RF, Robers MB, Wood KV, Sloan EK, Woolard J, Hill SJ. Cell Chem Biol. 2019 Jun 20;26(6):830-841.e9. doi: 10.1016/j.chembiol.2019.02.014. Epub 2019 Apr 4. PubMed
Small-Molecule Ferroptotic Agents with Potential to Selectively Target Cancer Stem Cells. Taylor WR, Fedorka SR, Gad I, Shah R, Alqahtani HD, Koranne R, Kuganesan N, Dlamini S, Rogers T, Al-Hamashi A, Kholodovych V, Barudi Y, Junk D, Rashid MS, Jackson MW, Tillekeratne LMV. Sci Rep. 2019 Apr 11;9(1):5926. doi: 10.1038/s41598-019-42251-5. PubMed
NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy. Princely Abudu Y, Pankiv S, Mathai BJ, Hakon Lystad A, Bindesboll C, Brenne HB, Yoke Wui Ng M, Thiede B, Yamamoto A, Mutugi Nthiga T, Lamark T, Esguerra CV, Johansen T, Simonsen A. Dev Cell. 2019 May 20;49(4):509-525.e12. doi: 10.1016/j.devcel.2019.03.013. Epub 2019 Apr 11. PubMed
Kruppel-like factor 5 (Klf5) regulates expression of mouse T1R1 amino acid receptor gene (Tas1r1) in C2C12 myoblast cells. Hirata Y, Toyono T, Kokabu S, Obikane Y, Kataoka S, Nakatomi M, Masaki C, Hosokawa R, Seta Y. Biomed Res. 2019;40(2):67-78. doi: 10.2220/biomedres.40.67. PubMed
BRCA1-associated R-loop affects transcription and differentiation in breast luminal epithelial cells. Chiang HC, Zhang X, Li J, Zhao X, Chen J, Wang HT, Jatoi I, Brenner A, Hu Y, Li R. Nucleic Acids Res. 2019 Jun 4;47(10):5086-5099. doi: 10.1093/nar/gkz262. PubMed
Dynamic enhancer partitioning instructs activation of a growth-related gene during exit from naive pluripotency. Greenberg M, Teissandier A, Walter M, Noordermeer D, Bourc'his D. Elife. 2019 Apr 16;8. pii: 44057. doi: 10.7554/eLife.44057. PubMed
Phosphoregulation of the oncogenic protein regulator of cytokinesis 1 (PRC1) by the atypical CDK16/CCNY complex. Hernandez-Ortega S, Sanchez-Botet A, Quandt E, Masip N, Gasa L, Verde G, Jimenez J, Levin RS, Rutaganira FU, Burlingame AL, Wolfgeher D, Ribeiro MPC, Kron SJ, Shokat KM, Clotet J. Exp Mol Med. 2019 Apr 16;51(4):44. doi: 10.1038/s12276-019-0242-2. PubMed
SIRT6 Is Responsible for More Efficient DNA Double-Strand Break Repair in Long-Lived Species. Tian X, Firsanov D, Zhang Z, Cheng Y, Luo L, Tombline G, Tan R, Simon M, Henderson S, Steffan J, Goldfarb A, Tam J, Zheng K, Cornwell A, Johnson A, Yang JN, Mao Z, Manta B, Dang W, Zhang Z, Vijg J, Wolfe A, Moody K, Kennedy BK, Bohmann D, Gladyshev VN, Seluanov A, Gorbunova V. Cell. 2019 Apr 18;177(3):622-638.e22. doi: 10.1016/j.cell.2019.03.043. PubMed
Hypoxia alters splicing of the cancer associated Fas gene. Peciuliene I, Vilys L, Jakubauskiene E, Zaliauskiene L, Kanopka A. Exp Cell Res. 2019 Jul 1;380(1):29-35. doi: 10.1016/j.yexcr.2019.04.015. Epub 2019 Apr 17. PubMed
Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation. Weiss-Sadan T, Itzhak G, Kaschani F, Yu Z, Mahameed M, Anaki A, Ben-Nun Y, Merquiol E, Tirosh B, Kessler B, Kaiser M, Blum G. Mol Cell Proteomics. 2019 Jul;18(7):1330-1344. doi: 10.1074/mcp.RA119.001392. Epub 2019 Apr 22. PubMed
Lamin B1 loss promotes lung cancer development and metastasis by epigenetic derepression of RET. Jia Y, Vong JS, Asafova A, Garvalov BK, Caputo L, Cordero J, Singh A, Boettger T, Gunther S, Fink L, Acker T, Barreto G, Seeger W, Braun T, Savai R, Dobreva G. J Exp Med. 2019 Jun 3;216(6):1377-1395. doi: 10.1084/jem.20181394. Epub 2019 Apr 23. PubMed
Pioneering function of Isl1 in the epigenetic control of cardiomyocyte cell fate. Gao R, Liang X, Cheedipudi S, Cordero J, Jiang X, Zhang Q, Caputo L, Gunther S, Kuenne C, Ren Y, Bhattacharya S, Yuan X, Barreto G, Chen Y, Braun T, Evans SM, Sun Y, Dobreva G. Cell Res. 2019 Jun;29(6):486-501. doi: 10.1038/s41422-019-0168-1. Epub 2019 Apr 25. PubMed
Synergy between Variant PRC1 Complexes Defines Polycomb-Mediated Gene Repression. Fursova NA, Blackledge NP, Nakayama M, Ito S, Koseki Y, Farcas AM, King HW, Koseki H, Klose RJ. Mol Cell. 2019 Jun 6;74(5):1020-1036.e8. doi: 10.1016/j.molcel.2019.03.024. Epub 2019 Apr 24. PubMed
Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities. Scelfo A, Fernandez-Perez D, Tamburri S, Zanotti M, Lavarone E, Soldi M, Bonaldi T, Ferrari KJ, Pasini D. Mol Cell. 2019 Jun 6;74(5):1037-1052.e7. doi: 10.1016/j.molcel.2019.04.002. Epub 2019 Apr 24. PubMed
Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency. Kalkan T, Bornelov S, Mulas C, Diamanti E, Lohoff T, Ralser M, Middelkamp S, Lombard P, Nichols J, Smith A. Cell Stem Cell. 2019 May 2;24(5):785-801.e7. doi: 10.1016/j.stem.2019.03.017. Epub 2019 Apr 25. PubMed
Guidelines for optimized gene knockout using CRISPR/Cas9. Campenhout CV, Cabochette P, Veillard AC, Laczik M, Zelisko-Schmidt A, Sabatel C, Dhainaut M, Vanhollebeke B, Gueydan C, Kruys V. Biotechniques. 2019 Jun;66(6):295-302. doi: 10.2144/btn-2018-0187. Epub 2019 May 1. PubMed
The quantitative relationship between isotopic and net contributions of lactate and glucose to the tricarboxylic acid (TCA) cycle. Ying M, Guo C, Hu X. J Biol Chem. 2019 Jun 14;294(24):9615-9630. doi: 10.1074/jbc.RA119.007841. Epub 2019 Apr 30. PubMed
Loss of Nuclear TDP-43 Is Associated with Decondensation of LINE Retrotransposons. Liu EY, Russ J, Cali CP, Phan JM, Amlie-Wolf A, Lee EB. Cell Rep. 2019 Apr 30;27(5):1409-1421.e6. doi: 10.1016/j.celrep.2019.04.003. PubMed
CD8(+) T cells regulate tumour ferroptosis during cancer immunotherapy. Wang W, Green M, Choi JE, Gijon M, Kennedy PD, Johnson JK, Liao P, Lang X, Kryczek I, Sell A, Xia H, Zhou J, Li G, Li J, Li W, Wei S, Vatan L, Zhang H, Szeliga W, Gu W, Liu R, Lawrence TS, Lamb C, Tanno Y, Cieslik M, Stone E, Georgiou G, Chan TA, Chinnaiyan A, Zou W. Nature. 2019 May;569(7755):270-274. doi: 10.1038/s41586-019-1170-y. Epub 2019 May 1. PubMed
Whole-genome sequencing reveals novel tandem-duplication hotspots and a prognostic mutational signature in gastric cancer. Xing R, Zhou Y, Yu J, Yu Y, Nie Y, Luo W, Yang C, Xiong T, Wu WKK, Li Z, Bing Y, Lin S, Zhang Y, Hu Y, Li L, Han L, Yang C, Huang S, Huang S, Zhou R, Li J, Wu K, Fan D, Tang G, Dou J, Zhu Z, Ji J, Fang X, Lu Y. Nat Commun. 2019 May 2;10(1):2037. doi: 10.1038/s41467-019-09644-6. PubMed
PATZ1 is required for efficient HIV-1 infection. Aziati ID, Yoshida T, Hamano A, Maeda K, Takeuchi H, Yamaoka S. Biochem Biophys Res Commun. 2019 Jun 25;514(2):538-544. doi: 10.1016/j.bbrc.2019.04.175. Epub 2019 May 3. PubMed
Targeted amplification of a sequence of interest in artificial chromosome in mammalian cells. Asoshina M, Myo G, Tada N, Tajino K, Shimizu N. Nucleic Acids Res. 2019 Jun 20;47(11):5998-6006. doi: 10.1093/nar/gkz343. PubMed
An acquired scaffolding function of the DNAJ-PKAc fusion contributes to oncogenic signaling in fibrolamellar carcinoma. Turnham RE, Smith FD, Kenerson HL, Omar MH, Golkowski M, Garcia I, Bauer R, Lau HT, Sullivan KM, Langeberg LK, Ong SE, Riehle KJ, Yeung RS, Scott JD. Elife. 2019 May 7;8. pii: 44187. doi: 10.7554/eLife.44187. PubMed
Generation of endogenous pH-sensitive EGF receptor and its application in high-throughput screening for proteins involved in clathrin-mediated endocytosis. Larsen MB, Perez Verdaguer M, Schmidt BF, Bruchez MP, Watkins SC, Sorkin A. Elife. 2019 May 8;8. pii: 46135. doi: 10.7554/eLife.46135. PubMed
Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development. Ishiuchi T, Ohishi H, Sato T, Kamimura S, Yorino M, Abe S, Suzuki A, Wakayama T, Suyama M, Sasaki H. Cell Rep. 2019 May 7;27(6):1742-1754.e6. doi: 10.1016/j.celrep.2019.04.028. PubMed
Quantitative Temporal Proteomic Analysis of Vaccinia Virus Infection Reveals Regulation of Histone Deacetylases by an Interferon Antagonist. Soday L, Lu Y, Albarnaz JD, Davies CTR, Antrobus R, Smith GL, Weekes MP. Cell Rep. 2019 May 7;27(6):1920-1933.e7. doi: 10.1016/j.celrep.2019.04.042. PubMed
Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology. Warner BK, Alder JK, Suli A. Methods Mol Biol. 2019;1965:235-250. doi: 10.1007/978-1-4939-9182-2_16. PubMed
CRISPR/Cas9-mediated gene knockout of ARID1A promotes primary progesterone resistance by downregulating progesterone receptor B in endometrial cancer cells. Wang H, Tang Z, Li T, Liu M, Li Y, Xing B. Oncol Res. 2019 May 9. doi: 10.3727/096504019X15561873320465. PubMed
Phosphorylation-dependent Regnase-1 release from endoplasmic reticulum is critical in IL-17 response. Tanaka H, Arima Y, Kamimura D, Tanaka Y, Takahashi N, Uehata T, Maeda K, Satoh T, Murakami M, Akira S. J Exp Med. 2019 Jun 3;216(6):1431-1449. doi: 10.1084/jem.20181078. Epub 2019 May 9. PubMed
MEK inhibitors activate Wnt signalling and induce stem cell plasticity in colorectal cancer. Zhan T, Ambrosi G, Wandmacher AM, Rauscher B, Betge J, Rindtorff N, Haussler RS, Hinsenkamp I, Bamberg L, Hessling B, Muller-Decker K, Erdmann G, Burgermeister E, Ebert MP, Boutros M. Nat Commun. 2019 May 16;10(1):2197. doi: 10.1038/s41467-019-09898-0. PubMed
GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor. Jewell JL, Fu V, Hong AW, Yu FX, Meng D, Melick CH, Wang H, Lam WM, Yuan HX, Taylor SS, Guan KL. Elife. 2019 May 21;8. pii: 43038. doi: 10.7554/eLife.43038. PubMed
Microhomologies are prevalent at Cas9-induced larger deletions. Owens DDG, Caulder A, Frontera V, Harman JR, Allan AJ, Bucakci A, Greder L, Codner GF, Hublitz P, McHugh PJ, Teboul L, de Bruijn MFTR. Nucleic Acids Res. 2019 May 25. pii: 5498629. doi: 10.1093/nar/gkz459. PubMed
p90 ribosomal S6 kinase (RSK) phosphorylates myosin phosphatase and thereby controls edge dynamics during cell migration. Samson SC, Elliott A, Mueller BD, Kim Y, Carney KR, Bergman JP, Blenis J, Mendoza MC. J Biol Chem. 2019 Jul 12;294(28):10846-10862. doi: 10.1074/jbc.RA119.007431. Epub 2019 May 28. PubMed
ERCC1/XPF Is Important for Repair of DNA Double-Strand Breaks Containing Secondary Structures. Li S, Lu H, Wang Z, Hu Q, Wang H, Xiang R, Chiba T, Wu X. iScience. 2019 Jun 28;16:63-78. doi: 10.1016/j.isci.2019.05.017. Epub 2019 May 16. PubMed
Species specific differences in use of ANP32 proteins by influenza A virus. Long JS, Idoko-Akoh A, Mistry B, Goldhill D, Staller E, Schreyer J, Ross C, Goodbourn S, Shelton H, Skinner MA, Sang H, McGrew MJ, Barclay W. Elife. 2019 Jun 4;8. pii: 45066. doi: 10.7554/eLife.45066. PubMed
Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing. Mishra A, Emamgholi F, Erlangga Z, Hartleben B, Unger K, Wolff K, Teichmann U, Kessel M, Woller N, Kuhnel F, Dow LE, Manns MP, Vogel A, Lowe SW, Saborowski A, Saborowski M. Carcinogenesis. 2019 Jun 6. pii: 5512083. doi: 10.1093/carcin/bgz108. PubMed
ASB6 Promotes the Stemness Properties and Sustains Metastatic Potential of Oral Squamous Cell Carcinoma Cells by Attenuating ER Stress. Hung KF, Liao PC, Chen CK, Chiu YT, Cheng DH, Kawasumi M, Kao SY, Lo JF. Int J Biol Sci. 2019 Apr 22;15(5):1080-1090. doi: 10.7150/ijbs.31484. eCollection 2019. PubMed
Hyaluronan synthesis supports glutamate transporter activity. Hayashi MK, Nishioka T, Shimizu H, Takahashi K, Kakegawa W, Mikami T, Hirayama Y, Koizumi S, Yoshida S, Yuzaki M, Tammi M, Sekino Y, Kaibuchi K, Shigemoto-Mogami Y, Yasui M, Sato K. J Neurochem. 2019 Aug;150(3):249-263. doi: 10.1111/jnc.14791. Epub 2019 Jul 9. PubMed
ZEB1 insufficiency causes corneal endothelial cell state transition and altered cellular processing. Frausto RF, Chung DD, Boere PM, Swamy VS, Duong HNV, Kao L, Azimov R, Zhang W, Carrigan L, Wong D, Morselli M, Zakharevich M, Hanser EM, Kassels AC, Kurtz I, Pellegrini M, Aldave AJ. PLoS One. 2019 Jun 13;14(6):e0218279. doi: 10.1371/journal.pone.0218279. eCollection 2019. PubMed
Modeling microcephaly with cerebral organoids reveals a WDR62-CEP170-KIF2A pathway promoting cilium disassembly in neural progenitors. Zhang W, Yang SL, Yang M, Herrlinger S, Shao Q, Collar JL, Fierro E, Shi Y, Liu A, Lu H, Herring BE, Guo ML, Buch S, Zhao Z, Xu J, Lu Z, Chen JF. Nat Commun. 2019 Jun 13;10(1):2612. doi: 10.1038/s41467-019-10497-2. PubMed
CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice. Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, Shimada K, Miyata H, Tobita T, Noda T, Castaneda JM, Kiyozumi D, Zhang Q, Larasati T, Young SAM, Kodani M, Huddleston CA, Robertson MJ, Coarfa C, Isotani A, Aitken RJ, Okabe M, Matzuk MM, Garcia TX, Ikawa M. Biol Reprod. 2019 Jun 14. pii: 5519226. doi: 10.1093/biolre/ioz103. PubMed
A novel histone H4 variant H4G regulates rDNA transcription in breast cancer. Long M, Sun X, Shi W, Yanru A, Leung STC, Ding D, Cheema MS, MacPherson N, Nelson CJ, Ausio J, Yan Y, Ishibashi T. Nucleic Acids Res. 2019 Jun 20. pii: 5521031. doi: 10.1093/nar/gkz547. PubMed
Functional Interrogation of Lynch Syndrome Associated MSH2 Missense Variants via CRISPR-Cas9 Gene Editing in Human Embryonic Stem Cells. Rath A, Mishra A, Ferreira VD, Hu C, Omerza G, Kelly K, Hesse A, Reddi HV, Grady JP, Heinen CD. Hum Mutat. 2019 Jun 25. doi: 10.1002/humu.23848. PubMed
HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis. Brandsma I, Sato K, van Rossum-Fikkert SE, van Vliet N, Sleddens E, Reuter M, Odijk H, van den Tempel N, Dekkers DHW, Bezstarosti K, Demmers JAA, Maas A, Lebbink J, Wyman C, Essers J, van Gent DC, Baarends WM, Knipscheer P, Kanaar R, Zelensky AN. Cell Rep. 2019 Jun 25;27(13):3790-3798.e7. doi: 10.1016/j.celrep.2019.05.096. PubMed
TRAF3 Is Required for NF-kappaB Pathway Activation Mediated by HTLV Tax Proteins. Fochi S, Bergamo E, Serena M, Mutascio S, Journo C, Mahieux R, Ciminale V, Bertazzoni U, Zipeto D, Romanelli MG. Front Microbiol. 2019 Jun 12;10:1302. doi: 10.3389/fmicb.2019.01302. eCollection 2019. PubMed
Enhancer-mediated enrichment of interacting JMJD3-DDX21 to ENPP2 locus prevents R-loop formation and promotes transcription. Argaud D, Boulanger MC, Chignon A, Mkannez G, Mathieu P. Nucleic Acids Res. 2019 Jun 28. pii: 5525060. doi: 10.1093/nar/gkz560. PubMed
Poly(ADP-ribose) polymerase-1 antagonizes DNA resection at double-strand breaks. Caron MC, Sharma AK, O'Sullivan J, Myler LR, Ferreira MT, Rodrigue A, Coulombe Y, Ethier C, Gagne JP, Langelier MF, Pascal JM, Finkelstein IJ, Hendzel MJ, Poirier GG, Masson JY. Nat Commun. 2019 Jul 4;10(1):2954. doi: 10.1038/s41467-019-10741-9. PubMed
Identification of the m(6)Am Methyltransferase PCIF1 Reveals the Location and Functions of m(6)Am in the Transcriptome. Boulias K, Toczydlowska-Socha D, Hawley BR, Liberman N, Takashima K, Zaccara S, Guez T, Vasseur JJ, Debart F, Aravind L, Jaffrey SR, Greer EL. Mol Cell. 2019 Jun 20. pii: S1097-2765(19)30439-3. doi: 10.1016/j.molcel.2019.06.006. PubMed
CATACOMB: An endogenous inducible gene that antagonizes H3K27 methylation activity of Polycomb repressive complex 2 via an H3K27M-like mechanism. Piunti A, Smith ER, Morgan MAJ, Ugarenko M, Khaltyan N, Helmin KA, Ryan CA, Murray DC, Rickels RA, Yilmaz BD, Rendleman EJ, Savas JN, Singer BD, Bulun SE, Shilatifard A. Sci Adv. 2019 Jul 3;5(7):eaax2887. doi: 10.1126/sciadv.aax2887. eCollection 2019 Jul. PubMed
Deletion of Tet proteins results in quantitative disparities during ESC differentiation partially attributable to alterations in gene expression. Reimer M Jr, Pulakanti K, Shi L, Abel A, Liang M, Malarkannan S, Rao S. BMC Dev Biol. 2019 Jul 8;19(1):16. doi: 10.1186/s12861-019-0196-6. PubMed
IL-1RA is part of the inflammasome-regulated immune response in bladder epithelial cells and influences colonization of uropathogenic E. coli. Lindblad A, Persson K, Demirel I. Cytokine. 2019 Nov;123:154772. doi: 10.1016/j.cyto.2019.154772. Epub 2019 Jul 9. PubMed
LC3-positive structures are prominent in autophagy-deficient cells. Runwal G, Stamatakou E, Siddiqi FH, Puri C, Zhu Y, Rubinsztein DC. Sci Rep. 2019 Jul 12;9(1):10147. doi: 10.1038/s41598-019-46657-z. PubMed
Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins. Agrotis A, von Chamier L, Oliver H, Kiso K, Singh T, Ketteler R. J Biol Chem. 2019 Jul 17. pii: AC119.009977. doi: 10.1074/jbc.AC119.009977. PubMed

Associated Plasmids

NQO1 targeting prodrug triggers innate sensing to overcome checkpoint blockade resistance. Li X, Liu Z, Zhang A, Han C, Shen A, Jiang L, Boothman DA, Qiao J, Wang Y, Huang X, Fu YX. Nat Commun. 2019 Jul 19;10(1):3251. doi: 10.1038/s41467-019-11238-1. PubMed
RNase L Induces Expression of A Novel Serine/Threonine Protein Kinase, DRAK1, to Promote Apoptosis. Manivannan P, Reddy V, Mukherjee S, Clark KN, Malathi K. Int J Mol Sci. 2019 Jul 19;20(14). pii: ijms20143535. doi: 10.3390/ijms20143535. PubMed
Depletion of phosphatidylinositol 4-phosphate at the Golgi translocates K-Ras to mitochondria. Miller TE, Henkels KM, Huddleston M, Salisbury R, Hussain SM, Sasaki AT, Cho KJ. J Cell Sci. 2019 Aug 22;132(16). pii: jcs.231886. doi: 10.1242/jcs.231886. PubMed
Wnt Signaling Modulates Routes of Retinoic Acid-Induced Differentiation of Embryonic Stem Cells. Wang S, Huang H, Xiang H, Gu B, Li W, Chen L, Zhang M. Stem Cells Dev. 2019 Oct 1;28(19):1334-1345. doi: 10.1089/scd.2019.0065. Epub 2019 Aug 27. PubMed
Identification of an emphysema-associated genetic variant near TGFB2 with regulatory effects in lung fibroblasts. Parker MM, Hao Y, Guo F, Pham B, Chase R, Platig J, Cho MH, Hersh CP, Thannickal VJ, Crapo J, Washko G, Randell SH, Silverman EK, San Jose Estepar R, Zhou X, Castaldi PJ. Elife. 2019 Jul 25;8. pii: 42720. doi: 10.7554/eLife.42720. PubMed
12-Lipoxygenase Regulates Cold Adaptation and Glucose Metabolism by Producing the Omega-3 Lipid 12-HEPE from Brown Fat. Leiria LO, Wang CH, Lynes MD, Yang K, Shamsi F, Sato M, Sugimoto S, Chen EY, Bussberg V, Narain NR, Sansbury BE, Darcy J, Huang TL, Kodani SD, Sakaguchi M, Rocha AL, Schulz TJ, Bartelt A, Hotamisligil GS, Hirshman MF, van Leyen K, Goodyear LJ, Bluher M, Cypess AM, Kiebish MA, Spite M, Tseng YH. Cell Metab. 2019 Oct 1;30(4):768-783.e7. doi: 10.1016/j.cmet.2019.07.001. Epub 2019 Jul 25. PubMed
Cell sensing and decision-making in confinement: The role of TRPM7 in a tug of war between hydraulic pressure and cross-sectional area. Zhao R, Afthinos A, Zhu T, Mistriotis P, Li Y, Serra SA, Zhang Y, Yankaskas CL, He S, Valverde MA, Sun SX, Konstantopoulos K. Sci Adv. 2019 Jul 24;5(7):eaaw7243. doi: 10.1126/sciadv.aaw7243. eCollection 2019 Jul. PubMed
KDM2 proteins constrain transcription from CpG island gene promoters independently of their histone demethylase activity. Turberfield AH, Kondo T, Nakayama M, Koseki Y, King HW, Koseki H, Klose RJ. Nucleic Acids Res. 2019 Sep 26;47(17):9005-9023. doi: 10.1093/nar/gkz607. PubMed
CRISPR/Cas9-Mediated Knockout and In Situ Inversion of the ORF57 Gene from All Copies of the Kaposi's Sarcoma-Associated Herpesvirus Genome in BCBL-1 Cells. BeltCappellino A, Majerciak V, Lobanov A, Lack J, Cam M, Zheng ZM. J Virol. 2019 Oct 15;93(21). pii: JVI.00628-19. doi: 10.1128/JVI.00628-19. Print 2019 Nov 1. PubMed
Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice. Fujihara Y, Noda T, Kobayashi K, Oji A, Kobayashi S, Matsumura T, Larasati T, Oura S, Kojima-Kita K, Yu Z, Matzuk MM, Ikawa M. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18498-18506. doi: 10.1073/pnas.1908736116. Epub 2019 Aug 27. PubMed
Addiction associated N40D mu-opioid receptor variant modulates synaptic function in human neurons. Halikere A, Popova D, Scarnati MS, Hamod A, Swerdel MR, Moore JC, Tischfield JA, Hart RP, Pang ZP. Mol Psychiatry. 2019 Sep 3. pii: 10.1038/s41380-019-0507-0. doi: 10.1038/s41380-019-0507-0. PubMed
ZCCHC8, the nuclear exosome targeting component, is mutated in familial pulmonary fibrosis and is required for telomerase RNA maturation. Gable DL, Gaysinskaya V, Atik CC, Talbot CC Jr, Kang B, Stanley SE, Pugh EW, Amat-Codina N, Schenk KM, Arcasoy MO, Brayton C, Florea L, Armanios M. Genes Dev. 2019 Oct 1;33(19-20):1381-1396. doi: 10.1101/gad.326785.119. Epub 2019 Sep 5. PubMed
Functional diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering. van der Schoot JMS, Fennemann FL, Valente M, Dolen Y, Hagemans IM, Becker AMD, Le Gall CM, van Dalen D, Cevirgel A, van Bruggen JAC, Engelfriet M, Caval T, Bentlage AEH, Fransen MF, Nederend M, Leusen JHW, Heck AJR, Vidarsson G, Figdor CG, Verdoes M, Scheeren FA. Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi: 10.1126/sciadv.aaw1822. eCollection 2019 Aug. PubMed

Associated Plasmids

Proteomics of Melanoma Response to Immunotherapy Reveals Mitochondrial Dependence. Harel M, Ortenberg R, Varanasi SK, Mangalhara KC, Mardamshina M, Markovits E, Baruch EN, Tripple V, Arama-Chayoth M, Greenberg E, Shenoy A, Ayasun R, Knafo N, Xu S, Anafi L, Yanovich-Arad G, Barnabas GD, Ashkenazi S, Besser MJ, Schachter J, Bosenberg M, Shadel GS, Barshack I, Kaech SM, Markel G, Geiger T. Cell. 2019 Sep 19;179(1):236-250.e18. doi: 10.1016/j.cell.2019.08.012. Epub 2019 Sep 5. PubMed
Comprehensive Interactome Analysis Reveals that STT3B Is Required for N-Glycosylation of Lassa Virus Glycoprotein. Zhu S, Wan W, Zhang Y, Shang W, Pan X, Zhang LK, Xiao G. J Virol. 2019 Nov 13;93(23). pii: JVI.01443-19. doi: 10.1128/JVI.01443-19. Print 2019 Dec 1. PubMed
The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex. Karakkat JV, Kaimala S, Sreedharan SP, Jayaprakash P, Adeghate EA, Ansari SA, Guccione E, Mensah-Brown EPK, Starling Emerald B. Nucleic Acids Res. 2019 Nov 4;47(19):10086-10103. doi: 10.1093/nar/gkz786. PubMed
Weak membrane interactions allow Rheb to activate mTORC1 signaling without major lysosome enrichment. Angarola B, Ferguson SM. Mol Biol Cell. 2019 Oct 15;30(22):2750-2760. doi: 10.1091/mbc.E19-03-0146. Epub 2019 Sep 18. PubMed

Associated Plasmids

Efficient ADCC killing of meningioma by avelumab and a high-affinity natural killer cell line, haNK. Giles AJ, Hao S, Padget M, Song H, Zhang W, Lynes J, Sanchez V, Liu Y, Jung J, Cao X, Fujii R, Jensen R, Gillespie D, Schlom J, Gilbert MR, Nduom EK, Yang C, Lee JH, Soon-Shiong P, Hodge JW, Park DM. JCI Insight. 2019 Oct 17;4(20). pii: 130688. doi: 10.1172/jci.insight.130688. PubMed
High-throughput genome-wide phenotypic screening via immunomagnetic cell sorting. Mair B, Aldridge PM, Atwal RS, Philpott D, Zhang M, Masud SN, Labib M, Tong AHY, Sargent EH, Angers S, Moffat J, Kelley SO. Nat Biomed Eng. 2019 Oct;3(10):796-805. doi: 10.1038/s41551-019-0454-8. Epub 2019 Sep 23. PubMed
DART-seq: an antibody-free method for global m(6)A detection. Meyer KD. Nat Methods. 2019 Sep 23. pii: 10.1038/s41592-019-0570-0. doi: 10.1038/s41592-019-0570-0. PubMed

Associated Plasmids

Synergistic effects of common schizophrenia risk variants. Schrode N, Ho SM, Yamamuro K, Dobbyn A, Huckins L, Matos MR, Cheng E, Deans PJM, Flaherty E, Barretto N, Topol A, Alganem K, Abadali S, Gregory J, Hoelzli E, Phatnani H, Singh V, Girish D, Aronow B, Mccullumsmith R, Hoffman GE, Stahl EA, Morishita H, Sklar P, Brennand KJ. Nat Genet. 2019 Oct;51(10):1475-1485. doi: 10.1038/s41588-019-0497-5. Epub 2019 Sep 23. PubMed
NF-kappaB Activation Promotes Alphavirus Replication in Mature Neurons. Yeh JX, Park E, Schultz KLW, Griffin DE. J Virol. 2019 Nov 26;93(24). pii: JVI.01071-19. doi: 10.1128/JVI.01071-19. Print 2019 Dec 15. PubMed
MUC16 in non-small cell lung cancer patients affected by familial lung cancer and indoor air pollution: clinical characteristics and cell behaviors. Chen Y, Huang Y, Kanwal M, Li G, Yang J, Niu H, Li Z, Ding X. Transl Lung Cancer Res. 2019 Aug;8(4):476-488. doi: 10.21037/tlcr.2019.07.10. PubMed
Double-strand breakage in the extrachromosomal double minutes triggers their aggregation in the nucleus, micronucleation, and morphological transformation. Oobatake Y, Shimizu N. Genes Chromosomes Cancer. 2020 Mar;59(3):133-143. doi: 10.1002/gcc.22810. Epub 2019 Oct 4. PubMed
Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in. Khan AO, White CW, Pike JA, Yule J, Slater A, Hill SJ, Poulter NS, Thomas SG, Morgan NV. Sci Rep. 2019 Oct 2;9(1):14219. doi: 10.1038/s41598-019-50733-9. PubMed
Keratin 14 is a novel interaction partner of keratinocyte differentiation regulator: receptor-interacting protein kinase 4. Sumer C, Boz Er AB, Dincer T. Turk J Biol. 2019 Aug 5;43(4):225-234. doi: 10.3906/biy-1904-37. eCollection 2019. PubMed
Evaluating the efficacy of small molecules for neural differentiation of common marmoset ESCs and iPSCs. Yoshimatsu S, Nakamura M, Nakajima M, Nemoto A, Sato T, Sasaki E, Shiozawa S, Okano H. Neurosci Res. 2019 Oct 3. pii: S0168-0102(19)30262-7. doi: 10.1016/j.neures.2019.09.005. PubMed

Associated Plasmids

Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. Laflamme C, McKeever PM, Kumar R, Schwartz J, Kolahdouzan M, Chen CX, You Z, Benaliouad F, Gileadi O, McBride HM, Durcan TM, Edwards AM, Healy LM, Robertson J, McPherson PS. Elife. 2019 Oct 15;8. pii: 48363. doi: 10.7554/eLife.48363. PubMed
Mutations in the sonic hedgehog pathway cause macrocephaly-associated conditions due to crosstalk to the PI3K/AKT/mTOR pathway. Klein SD, Nguyen DC, Bhakta V, Wong D, Chang VY, Davidson TB, Martinez-Agosto JA. Am J Med Genet A. 2019 Dec;179(12):2517-2531. doi: 10.1002/ajmg.a.61368. Epub 2019 Oct 22. PubMed
The protective effects of hydrogen sulfide on the myocardial ischemia via regulating Bmal1. Hu J, Xue Y, Tang K, Fan J, Du J, Li W, Chen S, Liu C, Ji W, Liang J, Zhuang J, Chen K. Biomed Pharmacother. 2019 Dec;120:109540. doi: 10.1016/j.biopha.2019.109540. Epub 2019 Oct 19. PubMed
The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation. Hildebrandt A, Bruggemann M, Ruckle C, Boerner S, Heidelberger JB, Busch A, Hanel H, Voigt A, Mockel MM, Ebersberger S, Scholz A, Dold A, Schmid T, Ebersberger I, Roignant JY, Zarnack K, Konig J, Beli P. Genome Biol. 2019 Oct 22;20(1):216. doi: 10.1186/s13059-019-1814-0. PubMed
Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations. Grajcarek J, Monlong J, Nishinaka-Arai Y, Nakamura M, Nagai M, Matsuo S, Lougheed D, Sakurai H, Saito MK, Bourque G, Woltjen K. Nat Commun. 2019 Oct 24;10(1):4856. doi: 10.1038/s41467-019-12829-8. PubMed
Two conserved glycine residues in mammalian and Dictyostelium Rictor are required for mTORC2 activity and integrity. Pergolizzi B, Panuzzo C, Ali MS, Lo Iacono M, Levra Levron C, Ponzone L, Prelli M, Cilloni D, Calautti E, Bozzaro S, Bracco E. J Cell Sci. 2019 Nov 14;132(22). pii: jcs.236505. doi: 10.1242/jcs.236505. PubMed
Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies. Krohn L, Ozturk TN, Vanderperre B, Ouled Amar Bencheikh B, Ruskey JA, Laurent SB, Spiegelman D, Postuma RB, Arnulf I, Hu MTM, Dauvilliers Y, Hogl B, Stefani A, Monaca CC, Plazzi G, Antelmi E, Ferini-Strambi L, Heidbreder A, Rudakou U, Cochen De Cock V, Young P, Wolf P, Oliva P, Zhang XK, Greenbaum L, Liong C, Gagnon JF, Desautels A, Hassin-Baer S, Montplaisir JY, Dupre N, Rouleau GA, Fon EA, Trempe JF, Lamoureux G, Alcalay RN, Gan-Or Z. Ann Neurol. 2020 Jan;87(1):139-153. doi: 10.1002/ana.25629. Epub 2019 Nov 18. PubMed
CD146 Regulates Growth Factor-Induced mTORC2 Activity Independent of the PI3K and mTORC1 Pathways. Xu W, Hua H, Chiu YH, Li G, Zhi H, Yu Z, Ren F, Luo Y, Cui W. Cell Rep. 2019 Oct 29;29(5):1311-1322.e5. doi: 10.1016/j.celrep.2019.09.047. PubMed
Targeted DNA methylation of neurodegenerative disease genes via homology directed repair. Cali CP, Park DS, Lee EB. Nucleic Acids Res. 2019 Dec 16;47(22):11609-11622. doi: 10.1093/nar/gkz979. PubMed
MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition. Busch JD, Cipullo M, Atanassov I, Bratic A, Silva Ramos E, Schondorf T, Li X, Pearce SF, Milenkovic D, Rorbach J, Larsson NG. Cell Rep. 2019 Nov 5;29(6):1728-1738.e9. doi: 10.1016/j.celrep.2019.09.080. PubMed
Distinct IL-1alpha-responsive enhancers promote acute and coordinated changes in chromatin topology in a hierarchical manner. Weiterer SS, Meier-Soelch J, Georgomanolis T, Mizi A, Beyerlein A, Weiser H, Brant L, Mayr-Buro C, Jurida L, Beuerlein K, Muller H, Weber A, Tenekeci U, Dittrich-Breiholz O, Bartkuhn M, Nist A, Stiewe T, van IJcken WF, Riedlinger T, Schmitz ML, Papantonis A, Kracht M. EMBO J. 2020 Jan 2;39(1):e101533. doi: 10.15252/embj.2019101533. Epub 2019 Nov 7. PubMed
LDAF1 and Seipin Form a Lipid Droplet Assembly Complex. Chung J, Wu X, Lambert TJ, Lai ZW, Walther TC, Farese RV Jr. Dev Cell. 2019 Dec 2;51(5):551-563.e7. doi: 10.1016/j.devcel.2019.10.006. Epub 2019 Nov 7. PubMed
Galectin-1-driven T cell exclusion in the tumor endothelium promotes immunotherapy resistance. Nambiar DK, Aguilera T, Cao H, Kwok S, Kong C, Bloomstein J, Wang Z, Rangan VS, Jiang D, von Eyben R, Liang R, Agarwal S, Colevas AD, Korman A, Allen CT, Uppaluri R, Koong AC, Giaccia A, Le QT. J Clin Invest. 2019 Dec 2;129(12):5553-5567. doi: 10.1172/JCI129025. PubMed
CBX2-dependent transcriptional landscape: implications for human sex development and its defects. Sproll P, Eid W, Biason-Lauber A. Sci Rep. 2019 Nov 12;9(1):16552. doi: 10.1038/s41598-019-53006-7. PubMed
Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing. Garcia B, Lee J, Edraki A, Hidalgo-Reyes Y, Erwood S, Mir A, Trost CN, Seroussi U, Stanley SY, Cohn RD, Claycomb JM, Sontheimer EJ, Maxwell KL, Davidson AR. Cell Rep. 2019 Nov 12;29(7):1739-1746.e5. doi: 10.1016/j.celrep.2019.10.017. PubMed

Associated Plasmids

Sex-dependent VEGF expression underlies variations in human pluripotent stem cell to endothelial progenitor differentiation. Randolph LN, Bao X, Oddo M, Lian XL. Sci Rep. 2019 Nov 13;9(1):16696. doi: 10.1038/s41598-019-53054-z. PubMed
Persistent epigenetic memory impedes rescue of the telomeric phenotype in human ICF iPSCs following DNMT3B correction. Toubiana S, Gagliardi M, Papa M, Manco R, Tzukerman M, Matarazzo MR, Selig S. Elife. 2019 Nov 20;8. pii: 47859. doi: 10.7554/eLife.47859. PubMed
CRISPR/Cas9-Mediated Knock-Out of Kras(G12D) Mutated Pancreatic Cancer Cell Lines. Lentsch E, Li L, Pfeffer S, Ekici AB, Taher L, Pilarsky C, Grutzmann R. Int J Mol Sci. 2019 Nov 14;20(22). pii: ijms20225706. doi: 10.3390/ijms20225706. PubMed
Modeling Niemann-Pick disease type C in a human haploid cell line allows for patient variant characterization and clinical interpretation. Erwood S, Brewer RA, Bily TMI, Maino E, Zhou L, Cohn RD, Ivakine EA. Genome Res. 2019 Dec;29(12):2010-2019. doi: 10.1101/gr.250720.119. Epub 2019 Nov 21. PubMed
A Conserved Noncoding Locus Regulates Random Monoallelic Xist Expression across a Topological Boundary. Galupa R, Nora EP, Worsley-Hunt R, Picard C, Gard C, van Bemmel JG, Servant N, Zhan Y, El Marjou F, Johanneau C, Diabangouaya P, Le Saux A, Lameiras S, Pipoli da Fonseca J, Loos F, Gribnau J, Baulande S, Ohler U, Giorgetti L, Heard E. Mol Cell. 2020 Jan 16;77(2):352-367.e8. doi: 10.1016/j.molcel.2019.10.030. Epub 2019 Nov 20. PubMed
Comparisons of dual isogenic human iPSC pairs identify functional alterations directly caused by an epilepsy associated SCN1A mutation. Xie Y, Ng NN, Safrina OS, Ramos CM, Ess KC, Schwartz PH, Smith MA, O'Dowd DK. Neurobiol Dis. 2020 Feb;134:104627. doi: 10.1016/j.nbd.2019.104627. Epub 2019 Nov 28. PubMed
Protective role of c-Jun NH2-terminal kinase-associated leucine zipper protein (JLP) in curcumin-induced cancer cell death. Boldbaatar J, Gunarta IK, Suzuki R, Erdenebaatar P, Davaakhuu G, Hohjoh H, Yoshioka K. Biochem Biophys Res Commun. 2020 Feb 12;522(3):697-703. doi: 10.1016/j.bbrc.2019.11.154. Epub 2019 Nov 28. PubMed
Bcl-2-associated athanogene 5 (BAG5) regulates Parkin-dependent mitophagy and cell death. De Snoo ML, Friesen EL, Zhang YT, Earnshaw R, Dorval G, Kapadia M, O'Hara DM, Agapova V, Chau H, Pellerito O, Tang MY, Wang X, Schmitt-Ulms G, Durcan TM, Fon EA, Kalia LV, Kalia SK. Cell Death Dis. 2019 Dec 2;10(12):907. doi: 10.1038/s41419-019-2132-x. PubMed
Tight junction protein occludin regulates progenitor Self-Renewal and survival in developing cortex. Bendriem RM, Singh S, Aleem AA, Antonetti DA, Ross ME. Elife. 2019 Dec 3;8. pii: 49376. doi: 10.7554/eLife.49376. PubMed
Expression of 9-O- and 7,9-O-Acetyl Modified Sialic Acid in Cells and Their Effects on Influenza Viruses. Barnard KN, Wasik BR, LaClair JR, Buchholz DW, Weichert WS, Alford-Lawrence BK, Aguilar HC, Parrish CR. mBio. 2019 Dec 3;10(6). pii: mBio.02490-19. doi: 10.1128/mBio.02490-19. PubMed
Characterization of the activity, aggregation, and toxicity of heterodimers of WT and ALS-associated mutant Sod1. Brasil AA, de Carvalho MDC, Gerhardt E, Queiroz DD, Pereira MD, Outeiro TF, Eleutherio ECA. Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25991-26000. doi: 10.1073/pnas.1902483116. Epub 2019 Dec 3. PubMed

Associated Plasmids

Transcriptional factor Nrf2 is essential for aggresome formation during proteasome inhibition. Qin S, Jiang C, Gao J. Biomed Rep. 2019 Dec;11(6):241-252. doi: 10.3892/br.2019.1247. Epub 2019 Oct 21. PubMed
The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis. Creed TM, Baldeosingh R, Eberly CL, Schlee CS, Kim M, Cutler JA, Pandey A, Civin CI, Fossett NG, Kingsbury TJ. Development. 2020 Jan 3;147(1). pii: dev.177022. doi: 10.1242/dev.177022. PubMed
Molecular Insights into miRNA-Driven Resistance to 5-Fluorouracil and Oxaliplatin Chemotherapy: miR-23b Modulates the Epithelial-Mesenchymal Transition of Colorectal Cancer Cells Gasiule S, Dreize N, Kaupinis A, Razanskas R, Ciupas L, Stankevicius V, Kapustina Z, Laurinavicius A, Valius M, Vilkaitis G. J Clin Med. 2019 Dec 2;8(12). pii: jcm8122115. doi: 10.3390/jcm8122115. PubMed
Simultaneous Deletion of Virulence Factors and Insertion of Antigens into the Infectious Laryngotracheitis Virus Using NHEJ-CRISPR/Cas9 and Cre-Lox System for Construction of a Stable Vaccine Vector. Atasoy MO, Rohaim MA, Munir M. Vaccines (Basel). 2019 Dec 5;7(4). pii: vaccines7040207. doi: 10.3390/vaccines7040207. PubMed
Detecting single ORAI1 proteins within the plasma membrane reveals higher-order channel complexes. Alansary D, Peckys DB, Niemeyer BA, de Jonge N. J Cell Sci. 2020 Jan 3;133(1). pii: jcs.240358. doi: 10.1242/jcs.240358. PubMed
Atlas of quantitative single-base-resolution N(6)-methyl-adenine methylomes. Koh CWQ, Goh YT, Goh WSS. Nat Commun. 2019 Dec 10;10(1):5636. doi: 10.1038/s41467-019-13561-z. PubMed
BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFbeta responses and altered cell mechanics. Hiepen C, Jatzlau J, Hildebrandt S, Kampfrath B, Goktas M, Murgai A, Cuellar Camacho JL, Haag R, Ruppert C, Sengle G, Cavalcanti-Adam EA, Blank KG, Knaus P. PLoS Biol. 2019 Dec 11;17(12):e3000557. doi: 10.1371/journal.pbio.3000557. eCollection 2019 Dec. PubMed
MAIT Cells Promote Tumor Initiation, Growth, and Metastases via Tumor MR1. Yan J, Allen S, McDonald E, Das I, Mak JYW, Liu L, Fairlie DP, Meehan BS, Chen Z, Corbett AJ, Varelias A, Smyth MJ, Teng MWL. Cancer Discov. 2020 Jan;10(1):124-141. doi: 10.1158/2159-8290.CD-19-0569. Epub 2019 Dec 11. PubMed
Accurate measurement of fast endocytic recycling kinetics in real time. Jonker CTH, Deo C, Zager PJ, Tkachuk AN, Weinstein AM, Rodriguez-Boulan E, Lavis LD, Schreiner R. J Cell Sci. 2020 Jan 22;133(2). pii: jcs.231225. doi: 10.1242/jcs.231225. PubMed
R534C mutation in hERG causes a trafficking defect in iPSC-derived cardiomyocytes from patients with type 2 long QT syndrome. Mesquita FCP, Arantes PC, Kasai-Brunswick TH, Araujo DS, Gubert F, Monnerat G, Silva Dos Santos D, Neiman G, Leitao IC, Barbosa RAQ, Coutinho JL, Vaz IM, Dos Santos MN, Borgonovo T, Cruz FES, Miriuka S, Medei EH, Campos de Carvalho AC, Carvalho AB. Sci Rep. 2019 Dec 16;9(1):19203. doi: 10.1038/s41598-019-55837-w. PubMed
Congenital pituitary hypoplasia model demonstrates hypothalamic OTX2 regulation of pituitary progenitor cells. Matsumoto R, Suga H, Aoi T, Bando H, Fukuoka H, Iguchi G, Narumi S, Hasegawa T, Muguruma K, Ogawa W, Takahashi Y. J Clin Invest. 2020 Feb 3;130(2):641-654. doi: 10.1172/JCI127378. PubMed
Genetically Engineered Lung Cancer Cells for Analyzing Epithelial-Mesenchymal Transition. Kielbus M, Czapinski J, Kalafut J, Wos J, Stepulak A, Rivero-Muller A. Cells. 2019 Dec 15;8(12). pii: cells8121644. doi: 10.3390/cells8121644. PubMed
Ablation of Bscl2/seipin in hepatocytes does not cause metabolic dysfunction in congenital generalised lipodystrophy. Mcilroy GD, Mitchell SE, Han W, Delibegovic M, Rochford JJ. Dis Model Mech. 2020 Jan 17;13(1). pii: dmm.042655. doi: 10.1242/dmm.042655. PubMed
The MITF-SOX10 regulated long non-coding RNA DIRC3 is a melanoma tumour suppressor. Coe EA, Tan JY, Shapiro M, Louphrasitthiphol P, Bassett AR, Marques AC, Goding CR, Vance KW. PLoS Genet. 2019 Dec 27;15(12):e1008501. doi: 10.1371/journal.pgen.1008501. eCollection 2019 Dec. PubMed
PRC1 Catalytic Activity Is Central to Polycomb System Function. Blackledge NP, Fursova NA, Kelley JR, Huseyin MK, Feldmann A, Klose RJ. Mol Cell. 2019 Dec 26. pii: S1097-2765(19)30892-5. doi: 10.1016/j.molcel.2019.12.001. PubMed
TCDD inhibited the osteogenic differentiation of human fetal palatal mesenchymal cells through AhR and BMP-2/TGF-beta/Smad signaling. Liu X, Li X, Tao Y, Li N, Ji M, Zhang X, Chen Y, He Z, Yu K, Yu Z. Toxicology. 2020 Feb 15;431:152353. doi: 10.1016/j.tox.2019.152353. Epub 2019 Dec 27. PubMed
The structural basis for cohesin-CTCF-anchored loops. Li Y, Haarhuis JHI, Sedeno Cacciatore A, Oldenkamp R, van Ruiten MS, Willems L, Teunissen H, Muir KW, de Wit E, Rowland BD, Panne D. Nature. 2020 Feb;578(7795):472-476. doi: 10.1038/s41586-019-1910-z. Epub 2020 Jan 6. PubMed
APOBEC3B reporter myeloma cell lines identify DNA damage response pathways leading to APOBEC3B expression. Yamazaki H, Shirakawa K, Matsumoto T, Kazuma Y, Matsui H, Horisawa Y, Stanford E, Sarca AD, Shirakawa R, Shindo K, Takaori-Kondo A. PLoS One. 2020 Jan 8;15(1):e0223463. doi: 10.1371/journal.pone.0223463. eCollection 2020. PubMed
ST3GAL3, ST3GAL4, and ST3GAL6 differ in their regulation of biological functions via the specificities for the alpha2,3-sialylation of target proteins. Qi F, Isaji T, Duan C, Yang J, Wang Y, Fukuda T, Gu J. FASEB J. 2020 Jan;34(1):881-897. doi: 10.1096/fj.201901793R. Epub 2019 Nov 27. PubMed
Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2. Tanaka M, Shiota M, Koyama M, Nakayama J, Yashiro M, Semba K, Goda N. Monoclon Antib Immunodiagn Immunother. 2020 Feb;39(1):23-26. doi: 10.1089/mab.2019.0043. Epub 2020 Jan 9. PubMed
p53 Integrates Temporal WDR5 Inputs during Neuroectoderm and Mesoderm Differentiation of Mouse Embryonic Stem Cells. Li Q, Mao F, Zhou B, Huang Y, Zou Z, denDekker AD, Xu J, Hou S, Liu J, Dou Y, Rao RC. Cell Rep. 2020 Jan 14;30(2):465-480.e6. doi: 10.1016/j.celrep.2019.12.039. PubMed
Irp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modification. Santos MCFD, Anderson CP, Neschen S, Zumbrennen-Bullough KB, Romney SJ, Kahle-Stephan M, Rathkolb B, Gailus-Durner V, Fuchs H, Wolf E, Rozman J, de Angelis MH, Cai WM, Rajan M, Hu J, Dedon PC, Leibold EA. Nat Commun. 2020 Jan 15;11(1):296. doi: 10.1038/s41467-019-14004-5. PubMed
Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, Shiba K, Inaba K, Ikawa M. PLoS Genet. 2020 Jan 21;16(1):e1008585. doi: 10.1371/journal.pgen.1008585. eCollection 2020 Jan. PubMed
NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis. Zhang B, Tang Z, Li L, Lu LY. Cell Death Differ. 2020 Jul;27(7):2176-2190. doi: 10.1038/s41418-020-0493-4. Epub 2020 Jan 21. PubMed
IL-4 controls activated neutrophil FcgammaR2b expression and migration into inflamed joints. Panda SK, Wigerblad G, Jiang L, Jimenez-Andrade Y, Iyer VS, Shen Y, Boddul SV, Guerreiro-Cacais AO, Raposo B, Kasza Z, Wermeling F. Proc Natl Acad Sci U S A. 2020 Jan 24. pii: 1914186117. doi: 10.1073/pnas.1914186117. PubMed

Associated Plasmids

hnRNPDL Phase Separation Is Regulated by Alternative Splicing and Disease-Causing Mutations Accelerate Its Aggregation. Batlle C, Yang P, Coughlin M, Messing J, Pesarrodona M, Szulc E, Salvatella X, Kim HJ, Taylor JP, Ventura S. Cell Rep. 2020 Jan 28;30(4):1117-1128.e5. doi: 10.1016/j.celrep.2019.12.080. PubMed
Furry protein suppresses nuclear localization of yes-associated protein (YAP) by activating NDR kinase and binding to YAP. Irie K, Nagai T, Mizuno K. J Biol Chem. 2020 Mar 6;295(10):3017-3028. doi: 10.1074/jbc.RA119.010783. Epub 2020 Jan 29. PubMed
Ursodeoxycholic acid protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction through acting on ALDH1L2. Yang Y, Liu S, Gao H, Wang P, Zhang Y, Zhang A, Jia Z, Huang S. Free Radic Biol Med. 2020 May 20;152:821-837. doi: 10.1016/j.freeradbiomed.2020.01.182. Epub 2020 Jan 28. PubMed
Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression. Mejhert N, Kuruvilla L, Gabriel KR, Elliott SD, Guie MA, Wang H, Lai ZW, Lane EA, Christiano R, Danial NN, Farese RV Jr, Walther TC. Mol Cell. 2020 Mar 19;77(6):1251-1264.e9. doi: 10.1016/j.molcel.2020.01.014. Epub 2020 Feb 4. PubMed
IGF1-mediated human embryonic stem cell self-renewal recapitulates the embryonic niche. Wamaitha SE, Grybel KJ, Alanis-Lobato G, Gerri C, Ogushi S, McCarthy A, Mahadevaiah SK, Healy L, Lea RA, Molina-Arcas M, Devito LG, Elder K, Snell P, Christie L, Downward J, Turner JMA, Niakan KK. Nat Commun. 2020 Feb 7;11(1):764. doi: 10.1038/s41467-020-14629-x. PubMed
Multiplexed Knockouts in the Model Diatom Phaeodactylum by Episomal Delivery of a Selectable Cas9. Moosburner MA, Gholami P, McCarthy JK, Tan M, Bielinski VA, Allen AE. Front Microbiol. 2020 Jan 28;11:5. doi: 10.3389/fmicb.2020.00005. eCollection 2020. PubMed
CRISPR-Mediated Protein Tagging with Nanoluciferase to Investigate Native Chemokine Receptor Function and Conformational Changes. White CW, Caspar B, Vanyai HK, Pfleger KDG, Hill SJ. Cell Chem Biol. 2020 May 21;27(5):499-510.e7. doi: 10.1016/j.chembiol.2020.01.010. Epub 2020 Feb 12. PubMed
Development of a Facile Approach for Generating Chemically Modified CRISPR/Cas9 RNA. Scott T, Soemardy C, Morris KV. Mol Ther Nucleic Acids. 2020 Mar 6;19:1176-1185. doi: 10.1016/j.omtn.2020.01.004. Epub 2020 Jan 16. PubMed
The phosphatidylcholine transfer protein StarD7 is important for myogenic differentiation in mouse myoblast C2C12 cells and human primary skeletal myoblasts. Horibata Y, Mitsuhashi S, Shimizu H, Maejima S, Sakamoto H, Aoyama C, Ando H, Sugimoto H. Sci Rep. 2020 Feb 18;10(1):2845. doi: 10.1038/s41598-020-59444-y. PubMed
The extracellular domain of angulin-1 and palmitoylation of its cytoplasmic region are required for angulin-1 assembly at tricellular contacts. Oda Y, Sugawara T, Fukata Y, Izumi Y, Otani T, Higashi T, Fukata M, Furuse M. J Biol Chem. 2020 Mar 27;295(13):4289-4302. doi: 10.1074/jbc.RA119.010491. Epub 2020 Feb 20. PubMed
Matrix stiffness regulates endosomal escape of uropathogenic E. coli. Moorthy S, Byfield FJ, Janmey PA, Klein EA. Cell Microbiol. 2020 May;22(5):e13196. doi: 10.1111/cmi.13196. Epub 2020 Mar 4. PubMed
The role of Mediator and Little Elongation Complex in transcription termination. Takahashi H, Ranjan A, Chen S, Suzuki H, Shibata M, Hirose T, Hirose H, Sasaki K, Abe R, Chen K, He Y, Zhang Y, Takigawa I, Tsukiyama T, Watanabe M, Fujii S, Iida M, Yamamoto J, Yamaguchi Y, Suzuki Y, Matsumoto M, Nakayama KI, Washburn MP, Saraf A, Florens L, Sato S, Tomomori-Sato C, Conaway RC, Conaway JW, Hatakeyama S. Nat Commun. 2020 Feb 26;11(1):1063. doi: 10.1038/s41467-020-14849-1. PubMed
The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex. Koo CZ, Harrison N, Noy PJ, Szyroka J, Matthews AL, Hsia HE, Muller SA, Tushaus J, Goulding J, Willis K, Apicella C, Cragoe B, Davis E, Keles M, Malinova A, McFarlane TA, Morrison PR, Nguyen HTH, Sykes MC, Ahmed H, Di Maio A, Seipold L, Saftig P, Cull E, Pliotas C, Rubinstein E, Poulter NS, Briddon SJ, Holliday ND, Lichtenthaler SF, Tomlinson MG. J Biol Chem. 2020 Feb 28. pii: RA120.012601. doi: 10.1074/jbc.RA120.012601. PubMed
Cerebral organoid and mouse models reveal a RAB39b-PI3K-mTOR pathway-dependent dysregulation of cortical development leading to macrocephaly/autism phenotypes. Zhang W, Ma L, Yang M, Shao Q, Xu J, Lu Z, Zhao Z, Chen R, Chai Y, Chen JF. Genes Dev. 2020 Apr 1;34(7-8):580-597. doi: 10.1101/gad.332494.119. Epub 2020 Feb 27. PubMed
Regulation of the Transferrin Receptor Recycling in Hepatitis C Virus-Replicating Cells. Haberger V, Elgner F, Roos J, Bender D, Hildt E. Front Cell Dev Biol. 2020 Feb 11;8:44. doi: 10.3389/fcell.2020.00044. eCollection 2020. PubMed
53BP1 loss rescues embryonic lethality but not genomic instability of BRCA1 total knockout mice. Chen J, Li P, Song L, Bai L, Huen MSY, Liu Y, Lu LY. Cell Death Differ. 2020 Sep;27(9):2552-2567. doi: 10.1038/s41418-020-0521-4. Epub 2020 Mar 5. PubMed
Protein S-glutathionylation stimulate adipogenesis by stabilizing C/EBPbeta in 3T3L1 cells. Watanabe Y, Watanabe K, Fujioka D, Nakamura K, Nakamura T, Uematsu M, Bachschmid MM, Matsui R, Kugiyama K. FASEB J. 2020 Apr;34(4):5827-5837. doi: 10.1096/fj.201902575R. Epub 2020 Mar 5. PubMed
Novel role for CRK adaptor proteins as essential components of SRC/FAK signaling for epithelial-mesenchymal transition and colorectal cancer aggressiveness. Franke FC, Slusarenko BO, Engleitner T, Johannes W, Laschinger M, Rad R, Nitsche U, Janssen KP. Int J Cancer. 2020 Sep 15;147(6):1715-1731. doi: 10.1002/ijc.32955. Epub 2020 Mar 16. PubMed
Lysosomal degradation products induce Coxiella burnetii virulence. Newton P, Thomas DR, Reed SCO, Lau N, Xu B, Ong SY, Pasricha S, Madhamshettiwar PB, Edgington-Mitchell LE, Simpson KJ, Roy CR, Newton HJ. Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6801-6810. doi: 10.1073/pnas.1921344117. Epub 2020 Mar 9. PubMed
ATP7B knockout disturbs copper and lipid metabolism in Caco-2 cells. Guttmann S, Nadzemova O, Grunewald I, Lenders M, Brand E, Zibert A, Schmidt HH. PLoS One. 2020 Mar 10;15(3):e0230025. doi: 10.1371/journal.pone.0230025. eCollection 2020. PubMed
Polyethylenimine based magnetic nanoparticles mediated non-viral CRISPR/Cas9 system for genome editing. Rohiwal SS, Dvorakova N, Klima J, Vaskovicova M, Senigl F, Slouf M, Pavlova E, Stepanek P, Babuka D, Benes H, Ellederova Z, Stieger K. Sci Rep. 2020 Mar 12;10(1):4619. doi: 10.1038/s41598-020-61465-6. PubMed
Reporter gene knock-in into Marc-145 cells using CRISPR/Cas9-mediated homologous recombination. Chang Y, Shao J, Gao Y, Liu W, Gao Z, Hu Y, Chang H. Biotechnol Lett. 2020 Aug;42(8):1317-1325. doi: 10.1007/s10529-020-02860-x. Epub 2020 Mar 17. PubMed
Global Regulation of the Histone Mark H3K36me2 Underlies Epithelial Plasticity and Metastatic Progression. Yuan S, Natesan R, Sanchez-Rivera FJ, Li J, Bhanu NV, Yamazoe T, Lin JH, Merrell AJ, Sela Y, Thomas SK, Jiang Y, Plesset JB, Miller EM, Shi J, Garcia BA, Lowe SW, Asangani IA, Stanger BZ. Cancer Discov. 2020 Jun;10(6):854-871. doi: 10.1158/2159-8290.CD-19-1299. Epub 2020 Mar 18. PubMed
CRISPR/Cas9-Mediated Biallelic Knockout of IRX3 Reduces the Production and Survival of Somatic Cell-Cloned Bama Minipigs. Zhu X, Wei Y, Zhan Q, Yan A, Feng J, Liu L, Tang D. Animals (Basel). 2020 Mar 17;10(3). pii: ani10030501. doi: 10.3390/ani10030501. PubMed
A cardiomyocyte show of force: A fluorescent alpha-actinin reporter line sheds light on human cardiomyocyte contractility versus substrate stiffness. Ribeiro MC, Slaats RH, Schwach V, Rivera-Arbelaez JM, Tertoolen LGJ, van Meer BJ, Molenaar R, Mummery CL, Claessens MMAE, Passier R. J Mol Cell Cardiol. 2020 Apr;141:54-64. doi: 10.1016/j.yjmcc.2020.03.008. Epub 2020 Mar 20. PubMed
DDX39B interacts with the pattern recognition receptor pathway to inhibit NF-kappaB and sensitize to alkylating chemotherapy. Szymura SJ, Bernal GM, Wu L, Zhang Z, Crawley CD, Voce DJ, Campbell PA, Ranoa DE, Weichselbaum RR, Yamini B. BMC Biol. 2020 Mar 24;18(1):32. doi: 10.1186/s12915-020-0764-z. PubMed
Integrated analysis of miRNA and mRNA expression profiles in p53-edited PFF cells. Qiao C, Liu W, Jiang H, He M, Yang Q, Xing Y. Cell Cycle. 2020 Apr;19(8):949-959. doi: 10.1080/15384101.2020.1742852. Epub 2020 Mar 26. PubMed
Reversible promoter methylation determines fluctuating expression of acute phase proteins. Zhang SC, Wang MY, Feng JR, Chang Y, Ji SR, Wu Y. Elife. 2020 Mar 30;9. pii: 51317. doi: 10.7554/eLife.51317. PubMed
Identification of drug modifiers for RYR1-related myopathy using a multi-species discovery pipeline. Volpatti JR, Endo Y, Knox J, Groom L, Brennan S, Noche R, Zuercher WJ, Roy P, Dirksen RT, Dowling JJ. Elife. 2020 Mar 30;9. pii: 52946. doi: 10.7554/eLife.52946. PubMed
Effect of CELSR3 on the Cell Cycle and Apoptosis of Hepatocellular Carcinoma Cells. Xie Z, Dang Y, Wu H, He R, Ma J, Peng Z, Rong M, Li Z, Yang J, Jiang Y, Chen G, Yang L. J Cancer. 2020 Feb 21;11(10):2830-2844. doi: 10.7150/jca.39328. eCollection 2020. PubMed
SNP rs17079281 decreases lung cancer risk through creating an YY1-binding site to suppress DCBLD1 expression. Wang Y, Ma R, Liu B, Kong J, Lin H, Yu X, Wang R, Li L, Gao M, Zhou B, Mohan M, Yu H, Hou Z, Shen H, Qian B. Oncogene. 2020 May;39(20):4092-4102. doi: 10.1038/s41388-020-1278-4. Epub 2020 Mar 30. PubMed
Knockout of IRF7 Highlights its Modulator Function of Host Response Against Avian Influenza Virus and the Involvement of MAPK and TOR Signaling Pathways in Chicken. Kim TH, Kern C, Zhou H. Genes (Basel). 2020 Apr 2;11(4). pii: genes11040385. doi: 10.3390/genes11040385. PubMed
Human Embryonic Stem Cell-Derived Wilson's Disease Model for Screening Drug Efficacy. Kim D, Kim SB, Ryu JL, Hong H, Chang JH, Yoo TJ, Jin X, Park HJ, Han C, Lee BH, Choi JH, Yoo HW, Kim JH, Woo DH. Cells. 2020 Apr 2;9(4). pii: cells9040872. doi: 10.3390/cells9040872. PubMed
ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons. Willems J, de Jong APH, Scheefhals N, Mertens E, Catsburg LAE, Poorthuis RB, de Winter F, Verhaagen J, Meye FJ, MacGillavry HD. PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665. PubMed

Associated Plasmids

Gene-based association analysis reveals involvement of LAMA5 and cell adhesion pathways in nicotine dependence in African- and European-American samples. Fan R, Cui W, Chen J, Ma Y, Yang Z, Payne TJ, Ma JZ, Li MD. Addict Biol. 2020 Apr 13:e12898. doi: 10.1111/adb.12898. PubMed
The WW domains dictate isoform-specific regulation of YAP1 stability and pancreatic cancer cell malignancy. Guo Q, Quan M, Dong J, Bai J, Wang J, Han R, Wang W, Cai Y, Lv YQ, Chen Q, Xu H, Lyu HD, Deng L, Zhou D, Xiao X, De Langhe S, Billadeau DD, Lou Z, Zhang JS. Theranostics. 2020 Mar 15;10(10):4422-4436. doi: 10.7150/thno.42795. eCollection 2020. PubMed
Prss55 but not Prss51 is required for male fertility in micedagger. Kobayashi K, Endo T, Matsumura T, Lu Y, Yu Z, Matzuk MM, Ikawa M. Biol Reprod. 2020 Aug 4;103(2):223-234. doi: 10.1093/biolre/ioaa041. PubMed
Tollip coordinates Parkin-dependent trafficking of mitochondrial-derived vesicles. Ryan TA, Phillips EO, Collier CL, Jb Robinson A, Routledge D, Wood RE, Assar EA, Tumbarello DA. EMBO J. 2020 Jun 2;39(11):e102539. doi: 10.15252/embj.2019102539. Epub 2020 Apr 20. PubMed
beta-catenin-mediated Wnt signal transduction proceeds through an endocytosis-independent mechanism. Rim EY, Kinney LK, Nusse R. Mol Biol Cell. 2020 Jun 15;31(13):1425-1436. doi: 10.1091/mbc.E20-02-0114. Epub 2020 Apr 22. PubMed
An optimized toolbox for the optogenetic control of intracellular transport. Nijenhuis W, van Grinsven MMP, Kapitein LC. J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149. PubMed
Transgenic Overexpression of the Disordered Prion Protein N1 Fragment in Mice Does Not Protect Against Neurodegenerative Diseases Due to Impaired ER Translocation. Mohammadi B, Linsenmeier L, Shafiq M, Puig B, Galliciotti G, Giudici C, Willem M, Eden T, Koch-Nolte F, Lin YH, Tatzelt J, Glatzel M, Altmeppen HC. Mol Neurobiol. 2020 Jun;57(6):2812-2829. doi: 10.1007/s12035-020-01917-2. Epub 2020 May 4. PubMed
Up-Regulation of Activating Transcription Factor 3 in Human Fibroblasts Inhibits Melanoma Cell Growth and Migration Through a Paracrine Pathway. Zu T, Wen J, Xu L, Li H, Mi J, Li H, Brakebusch C, Fisher DE, Wu X. Front Oncol. 2020 Apr 21;10:624. doi: 10.3389/fonc.2020.00624. eCollection 2020. PubMed
Characterization of agonist-dependent somatostatin receptor subtype 2 trafficking in neuroendocrine cells. Alshafie W, Pan YE, Kreienkamp HJ, Stroh T. Endocrine. 2020 Sep;69(3):655-669. doi: 10.1007/s12020-020-02329-x. Epub 2020 May 7. PubMed
Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion. Damiano-Guercio J, Kurzawa L, Mueller J, Dimchev G, Schaks M, Nemethova M, Pokrant T, Bruhmann S, Linkner J, Blanchoin L, Sixt M, Rottner K, Faix J. Elife. 2020 May 11;9. pii: 55351. doi: 10.7554/eLife.55351. PubMed
Multiplex precise base editing in cynomolgus monkeys. Zhang W, Aida T, Del Rosario RCH, Wilde JJ, Ding C, Zhang X, Baloch Z, Huang Y, Tang Y, Li D, Lu H, Zhou Y, Jiang M, Xu D, Fang Z, Zheng Z, Huang Q, Feng G, Yang S. Nat Commun. 2020 May 11;11(1):2325. doi: 10.1038/s41467-020-16173-0. PubMed
Novel role of ASH1L histone methyltransferase in anaplastic thyroid carcinoma. Xu B, Qin T, Yu J, Giordano TJ, Sartor MA, Koenig RJ. J Biol Chem. 2020 Jun 26;295(26):8834-8845. doi: 10.1074/jbc.RA120.013530. Epub 2020 May 12. PubMed
Characterization of the ZFX family of transcription factors that bind downstream of the start site of CpG island promoters. Ni W, Perez AA, Schreiner S, Nicolet CM, Farnham PJ. Nucleic Acids Res. 2020 Jun 19;48(11):5986-6000. doi: 10.1093/nar/gkaa384. PubMed
YAP-Mediated Recruitment of YY1 and EZH2 Represses Transcription of Key Cell-Cycle Regulators. Hoxha S, Shepard A, Troutman S, Diao H, Doherty JR, Janiszewska M, Witwicki RM, Pipkin ME, Ja WW, Kareta MS, Kissil JL. Cancer Res. 2020 Jun 15;80(12):2512-2522. doi: 10.1158/0008-5472.CAN-19-2415. Epub 2020 May 14. PubMed
HDAC1 inhibition ameliorates TDP-43-induced cell death in vitro and in vivo. Sanna S, Esposito S, Masala A, Sini P, Nieddu G, Galioto M, Fais M, Iaccarino C, Cestra G, Crosio C. Cell Death Dis. 2020 May 14;11(5):369. doi: 10.1038/s41419-020-2580-3. PubMed
Pathological role of excessive DNA as a trigger of keratinocyte proliferation in psoriasis. Luo Y, Hara T, Kawashima A, Ishido Y, Suzuki S, Ishii N, Kambara T, Suzuki K. Clin Exp Immunol. 2020 May 16. doi: 10.1111/cei.13455. PubMed
GABRG2 Deletion Linked to Genetic Epilepsy with Febrile Seizures Plus Affects the Expression of GABAA Receptor Subunits and Other Genes at Different Temperatures. Li X, Guo S, Liu K, Zhang C, Chang H, Yang W, Rong S, Hu Q, Cui J, Wang F, Sun T. Neuroscience. 2020 Jul 1;438:116-136. doi: 10.1016/j.neuroscience.2020.04.049. Epub 2020 May 8. PubMed
Fam20C regulates protein secretion by Cab45 phosphorylation. Hecht TK, Blank B, Steger M, Lopez V, Beck G, Ramazanov B, Mann M, Tagliabracci V, von Blume J. J Cell Biol. 2020 Jun 1;219(6). pii: 151783. doi: 10.1083/jcb.201910089. PubMed
Generation of Transgenic Cloned Buffalo Embryos Harboring the EGFP Gene in the Y Chromosome Using CRISPR/Cas9-Mediated Targeted Integration. Zhao X, Nie J, Tang Y, He W, Xiao K, Pang C, Liang X, Lu Y, Zhang M. Front Vet Sci. 2020 Apr 23;7:199. doi: 10.3389/fvets.2020.00199. eCollection 2020. PubMed
BIX-01294 enhanced chemotherapy effect in gastric cancer by inducing GSDME-mediated pyroptosis. Deng BB, Jiao BP, Liu YJ, Li YR, Wang GJ. Cell Biol Int. 2020 Sep;44(9):1890-1899. doi: 10.1002/cbin.11395. Epub 2020 Jun 8. PubMed
Consequences of Lmna Exon 4 Mutations in Myoblast Function. Gomez-Dominguez D, Epifano C, Miguel F, Castano AG, Vilaplana-Marti B, Martin A, Amarilla-Quintana S, Bertrand AT, Bonne G, Ramon-Azcon J, Rodriguez-Milla MA, Perez de Castro I. Cells. 2020 May 21;9(5). pii: cells9051286. doi: 10.3390/cells9051286. PubMed
Constructing Auxin-Inducible Degron Mutants Using an All-in-One Vector. Yesbolatova A, Saito Y, Kanemaki MT. Pharmaceuticals (Basel). 2020 May 23;13(5). pii: ph13050103. doi: 10.3390/ph13050103. PubMed

Associated Plasmids

Regulation of poly(a)-specific ribonuclease activity by reversible lysine acetylation. Dejene EA, Li Y, Showkatian Z, Ling H, Seto E. J Biol Chem. 2020 Jul 24;295(30):10255-10270. doi: 10.1074/jbc.RA120.012552. Epub 2020 May 26. PubMed
Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing. Weisheit I, Kroeger JA, Malik R, Klimmt J, Crusius D, Dannert A, Dichgans M, Paquet D. Cell Rep. 2020 May 26;31(8):107689. doi: 10.1016/j.celrep.2020.107689. PubMed
Noncoding Y RNAs regulate the levels, subcellular distribution and protein interactions of their Ro60 autoantigen partner. Leng Y, Sim S, Magidson V, Wolin SL. Nucleic Acids Res. 2020 Jul 9;48(12):6919-6930. doi: 10.1093/nar/gkaa414. PubMed
The accessory subunit of human DNA polymerase gamma is required for mitochondrial DNA maintenance and is able to stabilize the catalytic subunit. Do Y, Matsuda S, Inatomi T, Nakada K, Yasukawa T, Kang D. Mitochondrion. 2020 Jul;53:133-139. doi: 10.1016/j.mito.2020.05.008. Epub 2020 May 26. PubMed
A RIG-I-like receptor directs antiviral responses to a bunyavirus and is antagonized by virus-induced blockade of TRIM25-mediated ubiquitination. Min YQ, Ning YJ, Wang H, Deng F. J Biol Chem. 2020 Jul 10;295(28):9691-9711. doi: 10.1074/jbc.RA120.013973. Epub 2020 May 29. PubMed
Regulatory sharing between estrogen receptor alpha bound enhancers. Carleton JB, Ginley-Hidinger M, Berrett KC, Layer RM, Quinlan AR, Gertz J. Nucleic Acids Res. 2020 Jul 9;48(12):6597-6610. doi: 10.1093/nar/gkaa454. PubMed
The polycomb group protein PCGF6 mediates germline gene silencing by recruiting histone-modifying proteins to target gene promoters. Liu M, Zhu Y, Xing F, Liu S, Xia Y, Jiang Q, Qin J. J Biol Chem. 2020 Jul 10;295(28):9712-9724. doi: 10.1074/jbc.RA119.012121. Epub 2020 Jun 1. PubMed
Allele-Specific Prevention of Nonsense-Mediated Decay in Cystic Fibrosis Using Homology-Independent Genome Editing. Erwood S, Laselva O, Bily TMI, Brewer RA, Rutherford AH, Bear CE, Ivakine EA. Mol Ther Methods Clin Dev. 2020 May 12;17:1118-1128. doi: 10.1016/j.omtm.2020.05.002. eCollection 2020 Jun 12. PubMed
Inhibition of ROCK2 alleviates renal fibrosis and the metabolic disorders in the proximal tubular epithelial cells. You R, Zhou W, Li Y, Zhang Y, Huang S, Jia Z, Zhang A. Clin Sci (Lond). 2020 Jun 26;134(12):1357-1376. doi: 10.1042/CS20200030. PubMed
Efficient CRISPR/Cas9-mediated gene editing in Guangdong small-ear spotted pig cells using an optimized electrotransfection method. Wei YY, Zhan QM, Zhu XX, Yan AF, Feng J, Liu L, Li JH, Tang DS. Biotechnol Lett. 2020 Jun 3. pii: 10.1007/s10529-020-02930-0. doi: 10.1007/s10529-020-02930-0. PubMed
Hippo-YAP signaling controls lineage differentiation of mouse embryonic stem cells through modulating the formation of super-enhancers. Sun X, Ren Z, Cun Y, Zhao C, Huang X, Zhou J, Hu R, Su X, Ji L, Li P, Mak KLK, Gao F, Yang Y, Xu H, Ding J, Cao N, Li S, Zhang W, Lan P, Sun H, Wang J, Yuan P. Nucleic Acids Res. 2020 Jul 27;48(13):7182-7196. doi: 10.1093/nar/gkaa482. PubMed
Immunoglobulin Class Switch Recombination Is Initiated by Rare Cytosine Deamination Events at Switch Regions. Kim A, Han L, Yu K. Mol Cell Biol. 2020 Jul 29;40(16). pii: MCB.00125-20. doi: 10.1128/MCB.00125-20. Print 2020 Jul 29. PubMed
Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation. Maurissen TL, Woltjen K. Nat Commun. 2020 Jun 8;11(1):2876. doi: 10.1038/s41467-020-16643-5. PubMed
Modeling and Rescue of RP2 Retinitis Pigmentosa Using iPSC-Derived Retinal Organoids. Lane A, Jovanovic K, Shortall C, Ottaviani D, Panes AB, Schwarz N, Guarascio R, Hayes MJ, Palfi A, Chadderton N, Farrar GJ, Hardcastle AJ, Cheetham ME. Stem Cell Reports. 2020 Jul 14;15(1):67-79. doi: 10.1016/j.stemcr.2020.05.007. Epub 2020 Jun 11. PubMed
Kir2.1 interactome mapping uncovers PKP4 as a modulator of the Kir2.1-regulated inward rectifier potassium currents. Park SS, Ponce-Balbuena D, Kuick R, Guerrero-Serna G, Yoon J, Mellacheruvu D, Conlon KP, Basrur V, Nesvizhskii AI, Jalife J, Rual JF. Mol Cell Proteomics. 2020 Jun 15. pii: RA120.002071. doi: 10.1074/mcp.RA120.002071. PubMed
BAP1 is a haploinsufficient tumor suppressor linking chronic pancreatitis to pancreatic cancer in mice. Perkail S, Andricovich J, Kai Y, Tzatsos A. Nat Commun. 2020 Jun 15;11(1):3018. doi: 10.1038/s41467-020-16589-8. PubMed
Intragenic recruitment of NF-kappaB drives splicing modifications upon activation by the oncogene Tax of HTLV-1. Ameur LB, Marie P, Thenoz M, Giraud G, Combe E, Claude JB, Lemaire S, Fontrodona N, Polveche H, Bastien M, Gessain A, Wattel E, Bourgeois CF, Auboeuf D, Mortreux F. Nat Commun. 2020 Jun 16;11(1):3045. doi: 10.1038/s41467-020-16853-x. PubMed
PARP1 Is Required for ATM-Mediated p53 Activation and p53-Mediated Gene Expression after Ionizing Radiation. Gajewski S, Hartwig A. Chem Res Toxicol. 2020 Jul 20;33(7):1933-1940. doi: 10.1021/acs.chemrestox.0c00130. Epub 2020 Jul 7. PubMed
LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome. Bozal-Basterra L, Gonzalez-Santamarta M, Muratore V, Bermejo-Arteagabeitia A, Da Fonseca C, Barroso-Gomila O, Azkargorta M, Iloro I, Pampliega O, Andrade R, Martin-Martin N, Branon TC, Ting AY, Rodriguez JA, Carracedo A, Elortza F, Sutherland JD, Barrio R. Elife. 2020 Jun 18;9. pii: 55957. doi: 10.7554/eLife.55957. PubMed
Inactivation of REV7 enhances chemosensitivity and overcomes acquired chemoresistance in testicular germ cell tumors. Sakurai Y, Ichinoe M, Yoshida K, Nakazato Y, Saito S, Satoh M, Nakada N, Sanoyama I, Umezawa A, Numata Y, Shi-Xu J, Ichihara M, Takahashi M, Murakumo Y. Cancer Lett. 2020 Oct 1;489:100-110. doi: 10.1016/j.canlet.2020.06.001. Epub 2020 Jun 15. PubMed
BRPF3-HUWE1-mediated regulation of MYST2 is required for differentiation and cell-cycle progression in embryonic stem cells. Cho HI, Kim MS, Lee J, Yoo BC, Kim KH, Choe KM, Jang YK. Cell Death Differ. 2020 Jun 18. pii: 10.1038/s41418-020-0577-1. doi: 10.1038/s41418-020-0577-1. PubMed
Genome-wide analysis in the mouse embryo reveals the importance of DNA methylation for transcription integrity. Dahlet T, Argueso Lleida A, Al Adhami H, Dumas M, Bender A, Ngondo RP, Tanguy M, Vallet J, Auclair G, Bardet AF, Weber M. Nat Commun. 2020 Jun 19;11(1):3153. doi: 10.1038/s41467-020-16919-w. PubMed
CRISPR/Cas9-mediated genome-edited mice reveal 10 testis-enriched genes are dispensable for male fecundity. Park S, Shimada K, Fujihara Y, Xu Z, Shimada K, Larasati T, Pratiwi P, Matzuk RM, Devlin DJ, Yu Z, Garcia TX, Matzuk MM, Ikawa M. Biol Reprod. 2020 Aug 4;103(2):195-204. doi: 10.1093/biolre/ioaa084. PubMed
Proteome-wide Interrogation of Small GTPases Regulated by N(6)-Methyladenosine Modulators. Yang YY, Yu K, Li L, Huang M, Wang Y. Anal Chem. 2020 Jul 21;92(14):10145-10152. doi: 10.1021/acs.analchem.0c02203. Epub 2020 Jul 7. PubMed
Enforced Activation of Enhancer RNAs In Situ through the dCas9 Synergistic Activation Mediator System. Liao Z, Lee JH, Krakowiak J, Wang R, Li W. J Vis Exp. 2020 Jun 5;(160). doi: 10.3791/61460. PubMed
mTOR and S6K1 drive polycystic kidney by the control of Afadin-dependent oriented cell division. Bonucci M, Kuperwasser N, Barbe S, Koka V, de Villeneuve D, Zhang C, Srivastava N, Jia X, Stokes MP, Bienaime F, Verkarre V, Lopez JB, Jaulin F, Pontoglio M, Terzi F, Delaval B, Piel M, Pende M. Nat Commun. 2020 Jun 24;11(1):3200. doi: 10.1038/s41467-020-16978-z. PubMed
CRISPR/Cas9-based genome editing in mice uncovers 13 testis- or epididymis-enriched genes individually dispensable for male reproductiondagger. Sun J, Lu Y, Nozawa K, Xu Z, Morohoshi A, Castaneda JM, Noda T, Miyata H, Abbasi F, Shawki HH, Takahashi S, Devlin DJ, Yu Z, Matzuk RM, Garcia TX, Matzuk MM, Ikawa M. Biol Reprod. 2020 Aug 4;103(2):183-194. doi: 10.1093/biolre/ioaa083. PubMed
Recognition of Semaphorin Proteins by P. sordellii Lethal Toxin Reveals Principles of Receptor Specificity in Clostridial Toxins. Lee H, Beilhartz GL, Kucharska I, Raman S, Cui H, Lam MHY, Liang H, Rubinstein JL, Schramek D, Julien JP, Melnyk RA, Taipale M. Cell. 2020 Jul 23;182(2):345-356.e16. doi: 10.1016/j.cell.2020.06.005. Epub 2020 Jun 25. PubMed
Spermine synthase and MYC cooperate to maintain colorectal cancer cell survival by repressing Bim expression. Guo Y, Ye Q, Deng P, Cao Y, He D, Zhou Z, Wang C, Zaytseva YY, Schwartz CE, Lee EY, Mark Evers B, Morris AJ, Liu S, She QB. Nat Commun. 2020 Jun 26;11(1):3243. doi: 10.1038/s41467-020-17067-x. PubMed
Evaluation of the CRISPR/Cas9 Genetic Constructs in Efficient Disruption of Porcine Genes for Xenotransplantation Purposes Along with an Assessment of the Off-Target Mutation Formation. Ryczek N, Hryhorowicz M, Lipinski D, Zeyland J, Slomski R. Genes (Basel). 2020 Jun 26;11(6). pii: genes11060713. doi: 10.3390/genes11060713. PubMed
Increased expression and retention of the secretory chaperone proSAAS following cell stress. Shakya M, Yildirim T, Lindberg I. Cell Stress Chaperones. 2020 Jul 20. pii: 10.1007/s12192-020-01128-7. doi: 10.1007/s12192-020-01128-7. PubMed
Atrx Deletion in Neurons Leads to Sexually Dimorphic Dysregulation of miR-137 and Spatial Learning and Memory Deficits. Tamming RJ, Dumeaux V, Jiang Y, Shafiq S, Langlois L, Ellegood J, Qiu LR, Lerch JP, Berube NG. Cell Rep. 2020 Jun 30;31(13):107838. doi: 10.1016/j.celrep.2020.107838. PubMed
Loss of TET2 Affects Proliferation and Drug Sensitivity through Altered Dynamics of Cell-State Transitions. Morinishi L, Kochanowski K, Levine RL, Wu LF, Altschuler SJ. Cell Syst. 2020 Jul 22;11(1):86-94.e5. doi: 10.1016/j.cels.2020.06.003. Epub 2020 Jul 2. PubMed
Aberrantly expressed HORMAD1 disrupts nuclear localization of MCM8-MCM9 complex and compromises DNA mismatch repair in cancer cells. Liu K, Wang Y, Zhu Q, Li P, Chen J, Tang Z, Shen Y, Cheng X, Lu LY, Liu Y. Cell Death Dis. 2020 Jul 9;11(7):519. doi: 10.1038/s41419-020-2736-1. PubMed
A novel distal convoluted tubule-specific Cre-recombinase driven by the NaCl cotransporter gene. Cornelius RJ, Sharma A, Su XT, Guo JJ, McMahon JA, Ellison DH, McMahon AP, McCormick JA. Am J Physiol Renal Physiol. 2020 Sep 1;319(3):F423-F435. doi: 10.1152/ajprenal.00101.2020. Epub 2020 Jul 13. PubMed
Ribosome collisions trigger cis-acting feedback inhibition of translation initiation. Juszkiewicz S, Slodkowicz G, Lin Z, Freire-Pritchett P, Peak-Chew SY, Hegde RS. Elife. 2020 Jul 13;9. pii: 60038. doi: 10.7554/eLife.60038. PubMed
Herpes Simplex Virus Type 2 Counteracts Neurite Outgrowth Repulsion During Infection In A Nerve Growth Factor-Dependent Manner. Kropp KA, Lopez-Munoz AD, Ritter B, Martin R, Rastrojo A, Srivaratharajan S, Dohner K, Dhingra A, Czechowicz JS, Nagel CH, Sodeik B, Alcami A, Viejo-Borbolla A. J Virol. 2020 Jul 15. pii: JVI.01370-20. doi: 10.1128/JVI.01370-20. PubMed
Disruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype. Thornton N, Karamatic Crew V, Tilley L, Green CA, Tay CL, Griffiths RE, Singleton BK, Spring F, Walser P, Alattar AG, Jones B, Laundy R, Storry JR, Moller M, Wall L, Charlewood R, Westhoff CM, Lomas-Francis C, Yahalom V, Feick U, Seltsam A, Mayer B, Olsson ML, Anstee DJ. Nat Commun. 2020 Jul 16;11(1):3569. doi: 10.1038/s41467-020-17060-4. PubMed
CRISPR/Cas9-mediated MSTN disruption accelerates the growth of Chinese Bama pigs. Zhu XX, Zhan QM, Wei YY, Yan AF, Feng J, Liu L, Lu SS, Tang DS. Reprod Domest Anim. 2020 Jul 17. doi: 10.1111/rda.13775. PubMed
Distinct and stage-specific contributions of TET1 and TET2 to stepwise cytosine oxidation in the transition from naive to primed pluripotency. Mulholland CB, Traube FR, Ugur E, Parsa E, Eckl EM, Schonung M, Modic M, Bartoschek MD, Stolz P, Ryan J, Carell T, Leonhardt H, Bultmann S. Sci Rep. 2020 Jul 21;10(1):12066. doi: 10.1038/s41598-020-68600-3. PubMed
CGG Repeat Expansion, and Elevated Fmr1 Transcription and Mitochondrial Copy Number in a New Fragile X PM Mouse Embryonic Stem Cell Model. Gazy I, Miller CJ, Kim GY, Usdin K. Front Cell Dev Biol. 2020 Jun 30;8:482. doi: 10.3389/fcell.2020.00482. eCollection 2020. PubMed

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