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Tollip coordinates Parkin-dependent trafficking of mitochondrial-derived vesicles.
Ryan TA, Phillips EO, Collier CL, Jb Robinson A, Routledge D, Wood RE, Assar EA, Tumbarello DA
EMBO J. 2020 Jun 2;39(11):e102539. doi: 10.15252/embj.2019102539. Epub 2020 Apr 20.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
178050GFP-TollipGFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.
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178051GFP-Tollip M240A,F241AGFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.
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178054pIRESneo2 mycBioID-Tollipmyc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
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178055pIRESneo2 mycBioID-Tollip M240A,F241Amyc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
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196546pLentiCRISPRv1 sgTollipLentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.
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Antibodies from Article