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Stably Integrating an Inducible CRISPR-Cas9 to Protect Against Viral Infections in Vitro.
Madireddy I, Pierson Smela M
MicroPubl Biol. 2022 Jun 16;2022. doi: 10.17912/micropub.biology.000590. eCollection 2022.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
179913pX459-sg-mGreenLantern/EGFPAll in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.
179914pX459-sg-mGreenLantern332All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.
179915pX459-sg-CMV51All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.
179916pX459-sg-CMV152All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.
179917pX459-sg-CMV162All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.
179918pX459-sg-tdTomato90/816Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.
179919pX459-sg-tdTomato166/892Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.
188482rtTA-sg-mGreenLantern/EGFPgRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.
188483rtTA-sg-mGreenLantern332gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.
188484rtTA-sg-CMV51gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.
188485rtTA-sg-CMV152gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.
188486rtTA-sg-CMV162gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.
188487rtTA-sg-tdTomato90/816gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.
188488rtTA-sg-tdTomato166/892gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

Antibodies from Article