pEN396-pCAGGS-TIR1-V5-2A-HygroR
(Plasmid
#195102)
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PurposeVector to introduce osTir1 in cells. Hygromycin selection (2A-fusion). Auxin-inducible degron system.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 195102 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEN396 (addgene 92142)
- Total vector size (bp) 9438
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTIR1
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SpeciesOryza sativa
- Promoter pCAGGS
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGCATGTTAGCAGACTTCCTCT
- 3′ sequencing primer AACCATGTTCATGCCTTCTTCTTTT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.07.29.454218v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEN396-pCAGGS-TIR1-V5-2A-HygroR was a gift from Job Dekker (Addgene plasmid # 195102 ; http://n2t.net/addgene:195102 ; RRID:Addgene_195102) -
For your References section:
A cohesin traffic pattern genetically linked to gene regulation. Valton AL, Venev SV, Mair B, Khokhar ES, Tong AHY, Usaj M, Chan K, Pai AA, Moffat J, Dekker J. Nat Struct Mol Biol. 2022 Dec 8. doi: 10.1038/s41594-022-00890-9. 10.1038/s41594-022-00890-9 PubMed 36482254
Map uploaded by the depositor.
