Addgene: A cohesin traffic pattern genetically linked to gene regulation.

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Valton et al

A cohesin traffic pattern genetically linked to gene regulation.
Valton AL, Venev SV, Mair B, Khokhar ES, Tong AHY, Usaj M, Chan K, Pai AA, Moffat J, Dekker J
Nat Struct Mol Biol. 2022 Dec 8. doi: 10.1038/s41594-022-00890-9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
195101	221-pCAGGS-HA-AID-CTCFcDNA-AID-eGFP-2A-blasticidin	Vector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system.
195102	pEN396-pCAGGS-TIR1-V5-2A-HygroR	Vector to introduce osTir1 in cells. Hygromycin selection (2A-fusion). Auxin-inducible degron system.
195103	pX459-sgRNA1-CTCF-prom	Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).
195104	pX459-sgRNA2-CTCF-prom	Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).
195105	pX459-sgRNA3-CTCF-3p-UTR	Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).
195106	pX459-sgRNA4-CTCF-3p-UTR	Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).
195107	221-RPB1-AID-eGFP-2A-blasticidin	Targeting vector to introduce AID-eGFP tags in C-terminal of the human RPB1 gene. Blasticidin selection (2A-fusion). Auxin-inducible degron system.
195108	221-AID-RPB1	Targeting vector to introduce AID tag in N-terminal of the human RPB1 gene. Auxin-inducible degron system.
195109	pX459-sgRNA2-2-RPB1-C-term	Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).
195110	pX459-sgRNA1-RPB1-N-term	Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).
195111	pX459-sgRNA2-RPB1-N-term	Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).
195112	pX459-sgRNA3-RPB1-N-term	Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Antibodies from Article