pBA-mKeima-PIM
(Plasmid
#174650)
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PurposeLow level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174650 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneB-actin
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namexXFKBP homo-mKeima-2x FKBP homo-2x FKBP
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SpeciesSynthetic
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Insert Size (bp)2687
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MutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second homo-FKBP domain
- Promoter Chicken beta-actin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal plasmid ID: GK21 bioRxiv 2021.04.21
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBA-mKeima-PIM was a gift from Lukas Kapitein (Addgene plasmid # 174650 ; http://n2t.net/addgene:174650 ; RRID:Addgene_174650) -
For your References section:
Direct observation of aggregate-triggered selective autophagy in human cells. Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC. J Cell Sci. 2021 Oct 1;134(19). pii: 271972. doi: 10.1242/jcs.258824. Epub 2021 Oct 6. 10.1242/jcs.258824 PubMed 34447998
