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Direct observation of aggregate-triggered selective autophagy in human cells.
Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC
J Cell Sci. 2021 Oct 1;134(19). pii: 271972. doi: 10.1242/jcs.258824. Epub 2021 Oct 6. PubMed Article

Plasmids from Article

ID Plasmid Purpose
174650pBA-mKeima-PIMLow level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.
174651pLVX-IRES-hygro-EGFP-DFCP1Vector for overexpression of EGFP-tagged DFCP1 with IRES for hygromycin selection.
174652pLVX-IRES-hygro-EGFP-Stx17Vector for overexpression of EGFP-tagged Stx17 with IRES for hygromycin selection.

Antibodies from Article