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Golden Gate Assembly of CRISPR gRNA expression array for simultaneously targeting multiple genes.
Vad-Nielsen J, Lin L, Bolund L, Nielsen AL, Luo Y
Cell Mol Life Sci. 2016 May 13.
PubMed

Plasmids from Article

ID Plasmid Purpose  
80784pMA-SpCas9-g1BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array
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80785pMA-SpCas9-g2BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array
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80786pMA-SpCas9-g3BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array
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80787pMA-SpCas9-g4BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array
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80788pMA-SpCas9-g5BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array
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80789pMA-SpCas9-g6BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array
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80790pMA-SpCas9-g7BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array
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80791pMA-SpCas9-g8BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array
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80792pMA-SpCas9-g9BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array
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80793pMA-SpCas9-g10BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array
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80794pMA-MsgRNA-EGFPFor insertion of gRNA array containing 11-30 gRNA modules
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