lenti-HA-mBec1
(Plasmid
#99505)
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Purpose3rd generation lentiviral expression of wild-type Becn1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99505 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCDH-CMV-MCS-EF1-copGFP-T2A-Puro
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Backbone manufacturerSystem Biosciences
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Vector typeLentiviral
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Selectable markersPuromycin ; copGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBeclin 1
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Alt nameBecn1
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SpeciesM. musculus (mouse)
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Entrez GeneBecn1 (a.k.a. Atg6)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer LNCX (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lenti-HA-mBec1 was a gift from Congcong He (Addgene plasmid # 99505 ; http://n2t.net/addgene:99505 ; RRID:Addgene_99505) -
For your References section:
A Becn1 mutation mediates hyperactive autophagic sequestration of amyloid oligomers and improved cognition in Alzheimer's disease. Rocchi A, Yamamoto S, Ting T, Fan Y, Sadleir K, Wang Y, Zhang W, Huang S, Levine B, Vassar R, He C. PLoS Genet. 2017 Aug 14;13(8):e1006962. doi: 10.1371/journal.pgen.1006962. eCollection 2017 Aug. PGENETICS-D-17-00681 [pii] PubMed 28806762
