Vimentin-pLJM5
(Plasmid
#189868)
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PurposeExpresses human Vimentin in mammalian cells
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Depositing Lab
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Depositing OrganizationHebrew University
Located in Israel -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 189868 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 189868-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 189868-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepLJM5
- Backbone size w/o insert (bp) 8009
- Total vector size (bp) 10172
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVimentin
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SpeciesH. sapiens (human)
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GenBank IDNM_003380.5
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Entrez GeneVIM
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Tag
/ Fusion Protein
- m-Cherry
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 189868-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 189868-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Vimentin-pLJM5 was a gift from Yoav Shaul (Addgene plasmid # 189868 ; http://n2t.net/addgene:189868 ; RRID:Addgene_189868) -
For your References section:
DPYSL2 interacts with JAK1 to mediate breast cancer cell migration. Abu Rmaileh A, Solaimuthu B, Khatib A, Lavi S, Tanna M, Hayashi A, Ben Yosef M, Lichtenstein M, Pillar N, Shaul YD. J Cell Biol. 2022 Jul 4;221(7). pii: 213220. doi: 10.1083/jcb.202106078. Epub 2022 May 16. 10.1083/jcb.202106078 PubMed 35575798
Map uploaded by the depositor.
