pBMN-puro-P2A-FRB-Fis1
(Plasmid #135297)

• Purpose: stably express FBR-Fis1 in mammalian cells with puromycin selection
• Depositing Lab: Richard Youle
• Publication: Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6. doi: 10.1016/j.molcel.2019.02.010. Epub 2019 Mar 7.

Backbone

• Vector backbone: pBMN-Z-LacZ
• Backbone size w/o insert (bp): 4938
• Total vector size (bp): 6156
• Modifications to backbone: LacZ is removed during cloning and puromycin selection marker is introduced
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: FRB-Fis1
• Alt name: CGI-135
• Alt name: TTC11
• Species: H. sapiens (human)
• Insert Size (bp): 183
• Mutation: Fis1 tail (aa 93-152)
• GenBank ID: 51024 NM_016068.3
• Entrez Gene: FIS1 (a.k.a. CGI-135, TTC11)
• Tag / Fusion Protein:
  • FRB (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTCGGCTTCACCGTCACC
• 3′ sequencing primer: AGGTGGGGTCTTTCATTCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry