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Addgene

pGW1-mCherry-EGFP-PIM
(Plasmid #111759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111759 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GW1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
  • Species
    Synthetic
  • Insert Size (bp)
    3456
  • Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second homo-FKBP domain
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGW1-mCherry-EGFP-PIM was a gift from Lukas Kapitein (Addgene plasmid # 111759 ; http://n2t.net/addgene:111759 ; RRID:Addgene_111759)

  • For your References section:

    Probing aggrephagy using chemically-induced protein aggregates. Janssen AFJ, Katrukha EA, van Straaten W, Verlhac P, Reggiori F, Kapitein LC. Nat Commun. 2018 Oct 12;9(1):4245. doi: 10.1038/s41467-018-06674-4. 10.1038/s41467-018-06674-4 PubMed 30315152
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