Purposeretrovirus construct for stable expressing mt-mKeima
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72342||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5632
- Total vector size (bp) 6517
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Speciesstony coral Montipora
Insert Size (bp)894
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer TGACCTGGGAAGCCTTGGCT
- 3′ sequencing primer TTGCCAAAAGACGGCAATAT (Common Sequencing Primers)
Please also cite the original paper describing the use of mt-mkeima by Dr. Atsushi Miyawaki's lab at RIKEN, Japan.
Hiroyuki Katayama et al, 2011. A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chemistry and Bioogy 18(8): 1042-1052
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCHAC-mt-mKeima was a gift from Richard Youle (Addgene plasmid # 72342)
For your References section:The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Lazarou M, Sliter DA, Kane LA, Sarraf SA, Wang C, Burman JL, Sideris DP, Fogel AI, Youle RJ. Nature. 2015 Aug 20;524(7565):309-14. doi: 10.1038/nature14893. Epub 2015 Aug 12. 10.1038/nature14893 PubMed 26266977
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.