lentiCas9-Blast Citations (633)

Originally described in: Improved vectors and genome-wide libraries for CRISPR screening.
Sanjana NE, Shalem O, Zhang F Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PubMed Journal

Articles Citing lentiCas9-Blast

Articles
Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PubMed Associated Plasmids lentiCRISPR v2 lentiCas9-Blast lentiGuide-Puro lentiCas9n(D10A)-Blast
Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. PubMed Associated Plasmids pXPR_011 pLX_311-Cas9
TRAF1 Coordinates Polyubiquitin Signaling to Enhance Epstein-Barr Virus LMP1-Mediated Growth and Survival Pathway Activation. Greenfeld H, Takasaki K, Walsh MJ, Ersing I, Bernhardt K, Ma Y, Fu B, Ashbaugh CW, Cabo J, Mollo SB, Zhou H, Li S, Gewurz BE. PLoS Pathog. 2015 May 21;11(5):e1004890. doi: 10.1371/journal.ppat.1004890. eCollection 2015 May. PubMed
Cell death during crisis is mediated by mitotic telomere deprotection. Hayashi MT, Cesare AJ, Rivera T, Karlseder J. Nature. 2015 Jun 25;522(7557):492-6. doi: 10.1038/nature14513. PubMed
BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE. Nature. 2015 Sep 16. doi: 10.1038/nature15521. PubMed Associated Plasmids lentiCas9-Venus lentiGuide-Crimson
Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease. Dahlman JE, Abudayyeh OO, Joung J, Gootenberg JS, Zhang F, Konermann S. Nat Biotechnol. 2015 Oct 5;33(11):1159-1161. doi: 10.1038/nbt.3390. PubMed
DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z. PubMed Associated Plasmids pDECKO_GFP pDECKO_Malat1_A pDECKO_Malat1_B pDECKO_Malat1_C pDECKO_Malat1_D More ...
The NF-kappaB subunit c-Rel regulates Bach2 tumour suppressor expression in B-cell lymphoma. Hunter JE, Butterworth JA, Zhao B, Sellier H, Campbell KJ, Thomas HD, Bacon CM, Cockell SJ, Gewurz BE, Perkins ND. Oncogene. 2015 Nov 2. doi: 10.1038/onc.2015.399. PubMed
Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers. Zhang X, Choi PS, Francis JM, Imielinski M, Watanabe H, Cherniack AD, Meyerson M. Nat Genet. 2016 Feb;48(2):176-82. doi: 10.1038/ng.3470. Epub 2015 Dec 14. PubMed
Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Dang Y, Jia G, Choi J, Ma H, Anaya E, Ye C, Shankar P, Wu H. Genome Biol. 2015 Dec 15;16:280. doi: 10.1186/s13059-015-0846-3. PubMed
Enhanced Store-Operated Calcium Entry Leads to Striatal Synaptic Loss in a Huntington's Disease Mouse Model. Wu J, Ryskamp DA, Liang X, Egorova P, Zakharova O, Hung G, Bezprozvanny I. J Neurosci. 2016 Jan 6;36(1):125-41. doi: 10.1523/JNEUROSCI.1038-15.2016. PubMed
CRISPR-Cas9 Can Inhibit HIV-1 Replication but NHEJ Repair Facilitates Virus Escape. Wang G, Zhao N, Berkhout B, Das AT. Mol Ther. 2016 Mar;24(3):522-6. doi: 10.1038/mt.2016.24. Epub 2016 Jan 22. PubMed
Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo. Yin H, Song CQ, Dorkin JR, Zhu LJ, Li Y, Wu Q, Park A, Yang J, Suresh S, Bizhanova A, Gupta A, Bolukbasi MF, Walsh S, Bogorad RL, Gao G, Weng Z, Dong Y, Koteliansky V, Wolfe SA, Langer R, Xue W, Anderson DG. Nat Biotechnol. 2016 Feb 1. doi: 10.1038/nbt.3471. PubMed Associated Plasmids J7AAV-HDR Fah.2 J8AAV-HDR Ctnnb1.1
A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs. Karlas A, Berre S, Couderc T, Varjak M, Braun P, Meyer M, Gangneux N, Karo-Astover L, Weege F, Raftery M, Schonrich G, Klemm U, Wurzlbauer A, Bracher F, Merits A, Meyer TF, Lecuit M. Nat Commun. 2016 May 12;7:11320. doi: 10.1038/ncomms11320. PubMed
Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. McDade JR, Waxmonsky NC, Swanson LE, Fan M. Curr Protoc Mol Biol. 2016 Jul 1;115:31.5.1-31.5.13. doi: 10.1002/cpmb.8. PubMed
A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Zhang R, Miner JJ, Gorman MJ, Rausch K, Ramage H, White JP, Zuiani A, Zhang P, Fernandez E, Zhang Q, Dowd KA, Pierson TC, Cherry S, Diamond MS. Nature. 2016 Jul 7;535(7610):164-8. doi: 10.1038/nature18625. Epub 2016 Jun 17. PubMed
A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis. Lambrus BG, Daggubati V, Uetake Y, Scott PM, Clutario KM, Sluder G, Holland AJ. J Cell Biol. 2016 Jul 18;214(2):143-53. doi: 10.1083/jcb.201604054. PubMed
Open conformers of HLA-F are high-affinity ligands of the activating NK-cell receptor KIR3DS1. Garcia-Beltran WF, Holzemer A, Martrus G, Chung AW, Pacheco Y, Simoneau CR, Rucevic M, Lamothe-Molina PA, Pertel T, Kim TE, Dugan H, Alter G, Dechanet-Merville J, Jost S, Carrington M, Altfeld M. Nat Immunol. 2016 Sep;17(9):1067-74. doi: 10.1038/ni.3513. Epub 2016 Jul 25. PubMed
Engagement of SLAMF3 enhances CD4+ T-cell sensitivity to IL-2 and favors regulatory T-cell polarization in systemic lupus erythematosus. Comte D, Karampetsou MP, Kis-Toth K, Yoshida N, Bradley SJ, Mizui M, Kono M, Solomon JR, Kyttaris VC, Tsokos GC. Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):9321-6. doi: 10.1073/pnas.1605081113. Epub 2016 Aug 1. PubMed
Negative Feedback Regulation of HIV-1 by Gene Editing Strategy. Kaminski R, Chen Y, Salkind J, Bella R, Young WB, Ferrante P, Karn J, Malcolm T, Hu W, Khalili K. Sci Rep. 2016 Aug 16;6:31527. doi: 10.1038/srep31527. PubMed
Affinity Purification of the Hepatitis C Virus Replicase Identifies Valosin-Containing Protein, a Member of the ATPases Associated with Diverse Cellular Activities Family, as an Active Virus Replication Modulator. Yi Z, Fang C, Zou J, Xu J, Song W, Du X, Pan T, Lu H, Yuan Z. J Virol. 2016 Oct 14;90(21):9953-9966. Print 2016 Nov 1. PubMed
Noncoding somatic and inherited single-nucleotide variants converge to promote ESR1 expression in breast cancer. Bailey SD, Desai K, Kron KJ, Mazrooei P, Sinnott-Armstrong NA, Treloar AE, Dowar M, Thu KL, Cescon DW, Silvester J, Yang SY, Wu X, Pezo RC, Haibe-Kains B, Mak TW, Bedard PL, Pugh TJ, Sallari RC, Lupien M. Nat Genet. 2016 Oct;48(10):1260-6. doi: 10.1038/ng.3650. Epub 2016 Aug 29. PubMed
Frizzled proteins are colonic epithelial receptors for C. difficile toxin B. Tao L, Zhang J, Meraner P, Tovaglieri A, Wu X, Gerhard R, Zhang X, Stallcup WB, Miao J, He X, Hurdle JG, Breault DT, Brass AL, Dong M. Nature. 2016 Oct 20;538(7625):350-355. doi: 10.1038/nature19799. Epub 2016 Sep 28. PubMed
Functional receptor molecules CD300lf and CD300ld within the CD300 family enable murine noroviruses to infect cells. Haga K, Fujimoto A, Takai-Todaka R, Miki M, Doan YH, Murakami K, Yokoyama M, Murata K, Nakanishi A, Katayama K. Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):E6248-E6255. Epub 2016 Sep 28. PubMed
Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Goyal A, Myacheva K, Gross M, Klingenberg M, Duran Arque B, Diederichs S. Nucleic Acids Res. 2016 Sep 30. pii: gkw883. PubMed
The sigma-1 receptor mediates the beneficial effects of pridopidine in a mouse model of Huntington disease. Ryskamp D, Wu J, Geva M, Kusko R, Grossman I, Hayden M, Bezprozvanny I. Neurobiol Dis. 2017 Jan;97(Pt A):46-59. doi: 10.1016/j.nbd.2016.10.006. Epub 2016 Nov 3. PubMed
DPP8 and DPP9 inhibition induces pro-caspase-1-dependent monocyte and macrophage pyroptosis. Okondo MC, Johnson DC, Sridharan R, Go EB, Chui AJ, Wang MS, Poplawski SE, Wu W, Liu Y, Lai JH, Sanford DG, Arciprete MO, Golub TR, Bachovchin WW, Bachovchin DA. Nat Chem Biol. 2017 Jan;13(1):46-53. doi: 10.1038/nchembio.2229. Epub 2016 Nov 7. PubMed
STAT1 is essential for the inhibition of hepatitis C virus replication by interferon-lambda but not by interferon-alpha. Yamauchi S, Takeuchi K, Chihara K, Honjoh C, Kato Y, Yoshiki H, Hotta H, Sada K. Sci Rep. 2016 Dec 8;6:38336. doi: 10.1038/srep38336. PubMed
A Combinatorial CRISPR-Cas9 Attack on HIV-1 DNA Extinguishes All Infectious Provirus in Infected T Cell Cultures. Wang G, Zhao N, Berkhout B, Das AT. Cell Rep. 2016 Dec 13;17(11):2819-2826. doi: 10.1016/j.celrep.2016.11.057. PubMed
In vivo high-throughput profiling of CRISPR-Cpf1 activity. Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, Koh HC, Nam JW, Kim H. Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104. PubMed Associated Plasmids Lenti-AsCpf1-Blast Lenti-LbCpf1-Blast Lenti_gRNA-Puro
Pooled CRISPR screening with single-cell transcriptome readout. Datlinger P, Rendeiro AF, Schmidl C, Krausgruber T, Traxler P, Klughammer J, Schuster LC, Kuchler A, Alpar D, Bock C. Nat Methods. 2017 Jan 18. doi: 10.1038/nmeth.4177. PubMed Associated Plasmids CROPseq-Guide-Puro
YAP-IL-6ST autoregulatory loop activated on APC loss controls colonic tumorigenesis. Taniguchi K, Moroishi T, de Jong PR, Krawczyk M, Grebbin BM, Luo H, Xu RH, Golob-Schwarzl N, Schweiger C, Wang K, Di Caro G, Feng Y, Fearon ER, Raz E, Kenner L, Farin HF, Guan KL, Haybaeck J, Datz C, Zhang K, Karin M. Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1643-1648. doi: 10.1073/pnas.1620290114. Epub 2017 Jan 27. PubMed
Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Goyal A, Myacheva K, Gross M, Klingenberg M, Duran Arque B, Diederichs S. Nucleic Acids Res. 2017 Feb 17;45(3):e12. doi: 10.1093/nar/gkw883. PubMed
The CRISPR/Cas9 system efficiently reverts the tumorigenic ability of BCR/ABL in vitro and in a xenograft model of chronic myeloid leukemia. Garcia-Tunon I, Hernandez-Sanchez M, Ordonez JL, Alonso-Perez V, Alamo-Quijada M, Benito R, Guerrero C, Hernandez-Rivas JM, Sanchez-Martin M. Oncotarget. 2017 Apr 18;8(16):26027-26040. doi: 10.18632/oncotarget.15215. PubMed
Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Canver MC, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern EN, Needleman AJ, Galacteros F, Brugnara C, Kutlar A, McKenzie C, Reid M, Chen DD, Das PP, A Cole M, Zeng J, Kurita R, Nakamura Y, Yuan GC, Lettre G, Bauer DE, Orkin SH. Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793. PubMed Associated Plasmids lenti-Cas9-VQR-Blast lenti-Cas9-VQR-GFP_activity_reporter
Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer. Ohlund D, Handly-Santana A, Biffi G, Elyada E, Almeida AS, Ponz-Sarvise M, Corbo V, Oni TE, Hearn SA, Lee EJ, Chio II, Hwang CI, Tiriac H, Baker LA, Engle DD, Feig C, Kultti A, Egeblad M, Fearon DT, Crawford JM, Clevers H, Park Y, Tuveson DA. J Exp Med. 2017 Mar 6;214(3):579-596. doi: 10.1084/jem.20162024. Epub 2017 Feb 23. PubMed
TIRR regulates 53BP1 by masking its histone methyl-lysine binding function. Drane P, Brault ME, Cui G, Meghani K, Chaubey S, Detappe A, Parnandi N, He Y, Zheng XF, Botuyan MV, Kalousi A, Yewdell WT, Munch C, Harper JW, Chaudhuri J, Soutoglou E, Mer G, Chowdhury D. Nature. 2017 Mar 9;543(7644):211-216. doi: 10.1038/nature21358. Epub 2017 Feb 27. PubMed
Glucose Deprivation Induces ATF4-Mediated Apoptosis through TRAIL Death Receptors. Iurlaro R, Puschel F, Leon-Annicchiarico CL, O'Connor H, Martin SJ, Palou-Gramon D, Lucendo E, Munoz-Pinedo C. Mol Cell Biol. 2017 May 2;37(10). pii: e00479-16. doi: 10.1128/MCB.00479-16. Print 2017 May 15. PubMed
Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. PubMed Associated Plasmids lenti sgRNA(MS2)_puro optimized backbone lentiSAMv2 lentiMPH v2
USP9X regulates centrosome duplication and promotes breast carcinogenesis. Li X, Song N, Liu L, Liu X, Ding X, Song X, Yang S, Shan L, Zhou X, Su D, Wang Y, Zhang Q, Cao C, Ma S, Yu N, Yang F, Wang Y, Yao Z, Shang Y, Shi L. Nat Commun. 2017 Mar 31;8:14866. doi: 10.1038/ncomms14866. PubMed
Isolated polycystic liver disease genes define effectors of polycystin-1 function. Besse W, Dong K, Choi J, Punia S, Fedeles SV, Choi M, Gallagher AR, Huang EB, Gulati A, Knight J, Mane S, Tahvanainen E, Tahvanainen P, Sanna-Cherchi S, Lifton RP, Watnick T, Pei YP, Torres VE, Somlo S. J Clin Invest. 2017 May 1;127(5):1772-1785. doi: 10.1172/JCI90129. Epub 2017 Apr 4. PubMed
Pyroptosis and Apoptosis Pathways Engage in Bidirectional Crosstalk in Monocytes and Macrophages. Taabazuing CY, Okondo MC, Bachovchin DA. Cell Chem Biol. 2017 Apr 20;24(4):507-514.e4. doi: 10.1016/j.chembiol.2017.03.009. Epub 2017 Apr 6. PubMed
Contact sensitizers trigger human CD1-autoreactive T-cell responses. Betts RJ, Perkovic A, Mahapatra S, Del Bufalo A, Camara K, Howell AR, Martinozzi Teissier S, De Libero G, Mori L. Eur J Immunol. 2017 Jul;47(7):1171-1180. doi: 10.1002/eji.201746939. Epub 2017 May 23. PubMed
Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation. Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA, Ting AY. Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463. PubMed Associated Plasmids pLX_TRC208-SYNJ2BP-V5 pcDNA4 HisMax-V5-GFP-RRBP1 lentiGuide-RRBP1-1 lentiGuide-RRBP1-2 pLX304-Flag-APEX2-NES More ...
High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries. Strezoska Z, Perkett MR, Chou ET, Maksimova E, Anderson EM, McClelland S, Kelley ML, Vermeulen A, Smith AVB. J Biotechnol. 2017 Jun 10;251:189-200. doi: 10.1016/j.jbiotec.2017.04.017. Epub 2017 Apr 23. PubMed
Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos. Hodonsky CJ, Jain D, Schick UM, Morrison JV, Brown L, McHugh CP, Schurmann C, Chen DD, Liu YM, Auer PL, Laurie CA, Taylor KD, Browning BL, Li Y, Papanicolaou G, Rotter JI, Kurita R, Nakamura Y, Browning SR, Loos RJF, North KE, Laurie CC, Thornton TA, Pankratz N, Bauer DE, Sofer T, Reiner AP. PLoS Genet. 2017 Apr 28;13(4):e1006760. doi: 10.1371/journal.pgen.1006760. eCollection 2017 Apr. PubMed
CRISPR/Cas9 Screens Reveal Epstein-Barr Virus-Transformed B Cell Host Dependency Factors. Ma Y, Walsh MJ, Bernhardt K, Ashbaugh CW, Trudeau SJ, Ashbaugh IY, Jiang S, Jiang C, Zhao B, Root DE, Doench JG, Gewurz BE. Cell Host Microbe. 2017 May 10;21(5):580-591.e7. doi: 10.1016/j.chom.2017.04.005. PubMed
Hit and go CAS9 delivered through a lentiviral based self-limiting circuit. Petris G, Casini A, Montagna C, Lorenzin F, Prandi D, Romanel A, Zasso J, Conti L, Demichelis F, Cereseto A. Nat Commun. 2017 May 22;8:15334. doi: 10.1038/ncomms15334. PubMed
The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1-RUNX1T1. Yonezawa T, Takahashi H, Shikata S, Liu X, Tamura M, Asada S, Fukushima T, Fukuyama T, Tanaka Y, Sawasaki T, Kitamura T, Goyama S. J Biol Chem. 2017 Jul 28;292(30):12528-12541. doi: 10.1074/jbc.M117.785675. Epub 2017 May 23. PubMed
Peptide-specific engagement of the activating NK cell receptor KIR2DS1. Chapel A, Garcia-Beltran WF, Holzemer A, Ziegler M, Lunemann S, Martrus G, Altfeld M. Sci Rep. 2017 May 25;7(1):2414. doi: 10.1038/s41598-017-02449-x. PubMed
Aldolase B Overexpression is Associated with Poor Prognosis and Promotes Tumor Progression by Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma. Li Q, Li Y, Xu J, Wang S, Xu Y, Li X, Cai S. Cell Physiol Biochem. 2017;42(1):397-406. doi: 10.1159/000477484. Epub 2017 May 25. PubMed
Seed-effect modeling improves the consistency of genome-wide loss-of-function screens and identifies synthetic lethal vulnerabilities in cancer cells. Jaiswal A, Peddinti G, Akimov Y, Wennerberg K, Kuznetsov S, Tang J, Aittokallio T. Genome Med. 2017 Jun 1;9(1):51. doi: 10.1186/s13073-017-0440-2. PubMed
CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Mou H, Smith JL, Peng L, Yin H, Moore J, Zhang XO, Song CQ, Sheel A, Wu Q, Ozata DM, Li Y, Anderson DG, Emerson CP, Sontheimer EJ, Moore MJ, Weng Z, Xue W. Genome Biol. 2017 Jun 14;18(1):108. doi: 10.1186/s13059-017-1237-8. PubMed
Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts. Deb Roy A, Yin T, Choudhary S, Rodionov V, Pilbeam CC, Wu YI. Nat Commun. 2017 Jun 21;8:15831. doi: 10.1038/ncomms15831. PubMed
Integrative analysis of genomic alterations in triple-negative breast cancer in association with homologous recombination deficiency. Kawazu M, Kojima S, Ueno T, Totoki Y, Nakamura H, Kunita A, Qu W, Yoshimura J, Soda M, Yasuda T, Hama N, Saito-Adachi M, Sato K, Kohsaka S, Sai E, Ikemura M, Yamamoto S, Ogawa T, Fukayama M, Tada K, Seto Y, Morishita S, Hazama S, Shibata T, Yamashita Y, Mano H. PLoS Genet. 2017 Jun 21;13(6):e1006853. doi: 10.1371/journal.pgen.1006853. eCollection 2017 Jun. PubMed
Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus. Miles LA, Burga LN, Gardner EE, Bostina M, Poirier JT, Rudin CM. J Clin Invest. 2017 Jun 26. pii: 93472. doi: 10.1172/JCI93472. PubMed
Sam68 Regulates the Alternative Splicing of Survivin DEx3. Gaytan-Cervantes J, Gonzalez-Torres C, Maldonado V, Zampedri C, Ceballos-Cancino G, Melendez-Zajgla J. J Biol Chem. 2017 Jun 27. pii: jbc.M117.800318. doi: 10.1074/jbc.M117.800318. PubMed
mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer. Zabala-Letona A, Arruabarrena-Aristorena A, Martin-Martin N, Fernandez-Ruiz S, Sutherland JD, Clasquin M, Tomas-Cortazar J, Jimenez J, Torres I, Quang P, Ximenez-Embun P, Bago R, Ugalde-Olano A, Loizaga-Iriarte A, Lacasa-Viscasillas I, Unda M, Torrano V, Cabrera D, van Liempd SM, Cendon Y, Castro E, Murray S, Revandkar A, Alimonti A, Zhang Y, Barnett A, Lein G, Pirman D, Cortazar AR, Arreal L, Prudkin L, Astobiza I, Valcarcel-Jimenez L, Zuniga-Garcia P, Fernandez-Dominguez I, Piva M, Caro-Maldonado A, Sanchez-Mosquera P, Castillo-Martin M, Serra V, Beraza N, Gentilella A, Thomas G, Azkargorta M, Elortza F, Farras R, Olmos D, Efeyan A, Anguita J, Munoz J, Falcon-Perez JM, Barrio R, Macarulla T, Mato JM, Martinez-Chantar ML, Cordon-Cardo C, Aransay AM, Marks K, Baselga J, Tabernero J, Nuciforo P, Manning BD, Marjon K, Carracedo A. Nature. 2017 Jul 6;547(7661):109-113. doi: 10.1038/nature22964. Epub 2017 Jun 28. PubMed
CRISPR/Cas9-Mediated Scanning for Regulatory Elements Required for HPRT1 Expression via Thousands of Large, Programmed Genomic Deletions. Gasperini M, Findlay GM, McKenna A, Milbank JH, Lee C, Zhang MD, Cusanovich DA, Shendure J. Am J Hum Genet. 2017 Aug 3;101(2):192-205. doi: 10.1016/j.ajhg.2017.06.010. Epub 2017 Jul 14. PubMed
An erythroid-specific ATP2B4 enhancer mediates red blood cell hydration and malaria susceptibility. Lessard S, Gatof ES, Beaudoin M, Schupp PG, Sher F, Ali A, Prehar S, Kurita R, Nakamura Y, Baena E, Ledoux J, Oceandy D, Bauer DE, Lettre G. J Clin Invest. 2017 Aug 1;127(8):3065-3074. doi: 10.1172/JCI94378. Epub 2017 Jul 17. PubMed
A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout. Erard N, Knott SRV, Hannon GJ. Mol Cell. 2017 Jul 20;67(2):348-354.e4. doi: 10.1016/j.molcel.2017.06.030. PubMed
Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation. Konitsiotis AD, Rossmannek L, Stanoev A, Schmick M, Bastiaens PIH. Nat Commun. 2017 Jul 24;8(1):114. doi: 10.1038/s41467-017-00116-3. PubMed
Mitotic progression following DNA damage enables pattern recognition within micronuclei. Harding SM, Benci JL, Irianto J, Discher DE, Minn AJ, Greenberg RA. Nature. 2017 Aug 24;548(7668):466-470. doi: 10.1038/nature23470. Epub 2017 Jul 31. PubMed
A genome-wide CRISPR screen reconciles the role of N-linked glycosylation in galectin-3 transport to the cell surface. Stewart SE, Menzies SA, Popa SJ, Savinykh N, Petrunkina Harrison A, Lehner PJ, Moreau K. J Cell Sci. 2017 Oct 1;130(19):3234-3247. doi: 10.1242/jcs.206425. Epub 2017 Aug 3. PubMed
Decomposing Oncogenic Transcriptional Signatures to Generate Maps of Divergent Cellular States. Kim JW, Abudayyeh OO, Yeerna H, Yeang CH, Stewart M, Jenkins RW, Kitajima S, Konieczkowski DJ, Medetgul-Ernar K, Cavazos T, Mah C, Ting S, Van Allen EM, Cohen O, Mcdermott J, Damato E, Aguirre AJ, Liang J, Liberzon A, Alexe G, Doench J, Ghandi M, Vazquez F, Weir BA, Tsherniak A, Subramanian A, Meneses-Cime K, Park J, Clemons P, Garraway LA, Thomas D, Boehm JS, Barbie DA, Hahn WC, Mesirov JP, Tamayo P. Cell Syst. 2017 Aug 23;5(2):105-118.e9. doi: 10.1016/j.cels.2017.08.002. PubMed
DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes. Samwer M, Schneider MWG, Hoefler R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW. Cell. 2017 Aug 24;170(5):956-972.e23. doi: 10.1016/j.cell.2017.07.038. PubMed Associated Plasmids EGFP-BAF EGFP-P2A_BAF EGFP-P2A_BAF_G47E EGFP-P2A_BAF_L58R EGFP-BAF_L58R More ...
Creating a RAW264.7 CRISPR-Cas9 Genome Wide Library. Napier BA, Monack DM. Bio Protoc. 2017 May 20;7(10). doi: 10.21769/BioProtoc.2320. PubMed
Hyper-O-GlcNAcylation induces cisplatin resistance via regulation of p53 and c-Myc in human lung carcinoma. Luanpitpong S, Angsutararux P, Samart P, Chanthra N, Chanvorachote P, Issaragrisil S. Sci Rep. 2017 Sep 6;7(1):10607. doi: 10.1038/s41598-017-10886-x. PubMed
Combinatorial CRISPR-Cas9 and RNA Interference Attack on HIV-1 DNA and RNA Can Lead to Cross-Resistance. Zhao N, Wang G, Das AT, Berkhout B. Antimicrob Agents Chemother. 2017 Nov 22;61(12). pii: AAC.01486-17. doi: 10.1128/AAC.01486-17. Print 2017 Dec. PubMed
NCS-Rapgef2, the Protein Product of the Neuronal Rapgef2 Gene, Is a Specific Activator of D1 Dopamine Receptor-Dependent ERK Phosphorylation in Mouse Brain. Jiang SZ, Xu W, Emery AC, Gerfen CR, Eiden MV, Eiden LE. eNeuro. 2017 Sep 25;4(5). pii: eN-NWR-0248-17. doi: 10.1523/ENEURO.0248-17.2017. eCollection 2017 Sep-Oct. PubMed
Genome-Wide CRISPR Screen for Essential Cell Growth Mediators in Mutant KRAS Colorectal Cancers. Yau EH, Kummetha IR, Lichinchi G, Tang R, Zhang Y, Rana TM. Cancer Res. 2017 Nov 15;77(22):6330-6339. doi: 10.1158/0008-5472.CAN-17-2043. Epub 2017 Sep 27. PubMed
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A novel CHCHD10 mutation implicates a Mia40-dependent mitochondrial import deficit in ALS. Lehmer C, Schludi MH, Ransom L, Greiling J, Junghanel M, Exner N, Riemenschneider H, van der Zee J, Van Broeckhoven C, Weydt P, Heneka MT, Edbauer D. EMBO Mol Med. 2018 Jun;10(6). pii: emmm.201708558. doi: 10.15252/emmm.201708558. PubMed
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Targeting the HTLV-I-Regulated BATF3/IRF4 Transcriptional Network in Adult T Cell Leukemia/Lymphoma. Nakagawa M, Shaffer AL 3rd, Ceribelli M, Zhang M, Wright G, Huang DW, Xiao W, Powell J, Petrus MN, Yang Y, Phelan JD, Kohlhammer H, Dubois SP, Yoo HM, Bachy E, Webster DE, Yang Y, Xu W, Yu X, Zhao H, Bryant BR, Shimono J, Ishio T, Maeda M, Green PL, Waldmann TA, Staudt LM. Cancer Cell. 2018 Aug 13;34(2):286-297.e10. doi: 10.1016/j.ccell.2018.06.014. Epub 2018 Jul 26. PubMed
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A Single Point Mutation in the Rhinovirus 2B Protein Reduces the Requirement for Phosphatidylinositol 4-Kinase Class III Beta in Viral Replication. Roulin PS, Murer LP, Greber UF. J Virol. 2018 Nov 12;92(23). pii: JVI.01462-18. doi: 10.1128/JVI.01462-18. Print 2018 Dec 1. PubMed
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Reactive oxygen species mediate cancer stem-like cells and determine bortezomib sensitivity via Mcl-1 and Zeb-1 in mantle cell lymphoma. Luanpitpong S, Poohadsuan J, Samart P, Kiratipaiboon C, Rojanasakul Y, Issaragrisil S. Biochim Biophys Acta Mol Basis Dis. 2018 Nov;1864(11):3739-3753. doi: 10.1016/j.bbadis.2018.09.010. Epub 2018 Sep 11. PubMed
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Changes in mRNA abundance drive shuttling of RNA binding proteins, linking cytoplasmic RNA degradation to transcription. Gilbertson S, Federspiel JD, Hartenian E, Cristea IM, Glaunsinger B. Elife. 2018 Oct 3;7. pii: 37663. doi: 10.7554/eLife.37663. PubMed Associated Plasmids GFP-muSOX Thy1.1-muSOX Thy1.1-GFP Thy1.1-muSOX D219A
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Targeting E. coli invasion of the blood-brain barrier for investigating the pathogenesis and therapeutic development of E. coli meningitis. Zhu N, Liu W, Prakash A, Zhang C, Kim KS. Cell Microbiol. 2020 May 24:e13231. doi: 10.1111/cmi.13231. PubMed
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Cell Type-Specific In Vitro Gene Expression Profiling of Stem Cell-Derived Neural Models. Gregory JA, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, Barretto N, Schrode N, Akbalik G, Kang K, Cheng E, Bowles K, Lotz S, Goderie S, Karch CM, Temple S, Goate A, Brennand KJ, Phatnani H. Cells. 2020 Jun 5;9(6). pii: cells9061406. doi: 10.3390/cells9061406. PubMed Associated Plasmids P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo More ...
Massively parallel Cas13 screens reveal principles for guide RNA design. Wessels HH, Mendez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana NE. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16. PubMed Associated Plasmids pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE More ...
The Tumour Suppressor TMEM127 Is a Nedd4-Family E3 Ligase Adaptor Required by Salmonella SteD to Ubiquitinate and Degrade MHC Class II Molecules. Alix E, Godlee C, Cerny O, Blundell S, Tocci R, Matthews S, Liu M, Pruneda JN, Swatek KN, Komander D, Sleap T, Holden DW. Cell Host Microbe. 2020 Jul 8;28(1):54-68.e7. doi: 10.1016/j.chom.2020.04.024. Epub 2020 Jun 10. PubMed
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Sequential CRISPR-Based Screens Identify LITAF and CDIP1 as the Bacillus cereus Hemolysin BL Toxin Host Receptors. Liu J, Zuo Z, Sastalla I, Liu C, Jang JY, Sekine Y, Li Y, Pirooznia M, Leppla SH, Finkel T, Liu S. Cell Host Microbe. 2020 Sep 9;28(3):402-410.e5. doi: 10.1016/j.chom.2020.05.012. Epub 2020 Jun 15. PubMed
LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome. Bozal-Basterra L, Gonzalez-Santamarta M, Muratore V, Bermejo-Arteagabeitia A, Da Fonseca C, Barroso-Gomila O, Azkargorta M, Iloro I, Pampliega O, Andrade R, Martin-Martin N, Branon TC, Ting AY, Rodriguez JA, Carracedo A, Elortza F, Sutherland JD, Barrio R. Elife. 2020 Jun 18;9. pii: 55957. doi: 10.7554/eLife.55957. PubMed
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Identification of BLNK and BTK as mediators of rituximab-induced programmed cell death by CRISPR screens in GCB-subtype diffuse large B-cell lymphoma. Thomsen EA, Rovsing AB, Anderson MV, Due H, Huang J, Luo Y, Dybkaer K, Mikkelsen JG. Mol Oncol. 2020 Jun 25. doi: 10.1002/1878-0261.12753. PubMed
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A novel TRPM7/O-GlcNAc axis mediates tumour cell motility and metastasis by stabilising c-Myc and caveolin-1 in lung carcinoma. Luanpitpong S, Rodboon N, Samart P, Vinayanuwattikun C, Klamkhlai S, Chanvorachote P, Rojanasakul Y, Issaragrisil S. Br J Cancer. 2020 Jul 20. pii: 10.1038/s41416-020-0991-7. doi: 10.1038/s41416-020-0991-7. PubMed
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ABHD11 Is Critical for Embryonic Stem Cell Expansion, Differentiation and Lipid Metabolic Homeostasis. Liu G, Ruan Y, Zhang J, Wang X, Wu W, He P, Wang J, Xiong J, Cheng Y, Liu L, Yang Y, Tian Y, Jian R. Front Cell Dev Biol. 2020 Jul 7;8:570. doi: 10.3389/fcell.2020.00570. eCollection 2020. PubMed
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Loss of HPV type 16 E7 restores cGAS-STING responses in human papilloma virus-positive oropharyngeal squamous cell carcinomas cells. Bortnik V, Wu M, Julcher B, Salinas A, Nikolic I, Simpson KJ, McMillan NA, Idris A. J Microbiol Immunol Infect. 2020 Jul 30. pii: S1684-1182(20)30167-5. doi: 10.1016/j.jmii.2020.07.010. PubMed
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Truncated HDAC9 identified by integrated genome-wide screen as the key modulator for paclitaxel resistance in triple-negative breast cancer. Lian B, Pei YC, Jiang YZ, Xue MZ, Li DQ, Li XG, Zheng YZ, Liu XY, Qiao F, Sun WL, Ling H, He M, Yao L, Hu X, Shao ZM. Theranostics. 2020 Sep 2;10(24):11092-11109. doi: 10.7150/thno.44997. eCollection 2020. PubMed
Identification of the transcription factor Miz1 as an essential regulator of diphthamide biosynthesis using a CRISPR-mediated genome-wide screen. Liu J, Zuo Z, Zou M, Finkel T, Liu S. PLoS Genet. 2020 Oct 15;16(10):e1009068. doi: 10.1371/journal.pgen.1009068. eCollection 2020 Oct. PubMed
Triggering Receptor Expressed on Myeloid Cell 2 R47H Exacerbates Immune Response in Alzheimer's Disease Brain. Korvatska O, Kiianitsa K, Ratushny A, Matsushita M, Beeman N, Chien WM, Satoh JI, Dorschner MO, Keene CD, Bammler TK, Bird TD, Raskind WH. Front Immunol. 2020 Sep 25;11:559342. doi: 10.3389/fimmu.2020.559342. eCollection 2020. PubMed
Chromatin Looping Shapes KLF5-Dependent Transcriptional Programs in Human Epithelial Cancers. Liu Y, Guo B, Aguilera-Jimenez E, Chu VS, Zhou J, Wu Z, Francis JM, Yang X, Choi PS, Bailey SD, Zhang X. Cancer Res. 2020 Dec 15;80(24):5464-5477. doi: 10.1158/0008-5472.CAN-20-1287. Epub 2020 Oct 28. PubMed
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Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Wei J, Alfajaro MM, DeWeirdt PC, Hanna RE, Lu-Culligan WJ, Cai WL, Strine MS, Zhang SM, Graziano VR, Schmitz CO, Chen JS, Mankowski MC, Filler RB, Ravindra NG, Gasque V, de Miguel FJ, Patil A, Chen H, Oguntuyo KY, Abriola L, Surovtseva YV, Orchard RC, Lee B, Lindenbach BD, Politi K, van Dijk D, Kadoch C, Simon MD, Yan Q, Doench JG, Wilen CB. Cell. 2021 Jan 7;184(1):76-91.e13. doi: 10.1016/j.cell.2020.10.028. Epub 2020 Oct 20. PubMed Associated Plasmids Green monkey (Chlorocebus sabaeus) sgRNA library
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Engineered mini H1 promoters with dedicated RNA Polymerase II or III activity. Gao Z, van der Velden YU, Fan M, van der Linden CA, Vink M, Herrera-Carrillo E, Berkhout B. J Biol Chem. 2020 Nov 5. pii: S0021-9258(20)00012-5. doi: 10.1074/jbc.RA120.015386. PubMed Associated Plasmids H1.99.luc H1.99.M.luc H1.224.luc H1.224.M.luc H1.M10.luc More ...
TFEB Transcriptional Responses Reveal Negative Feedback by BHLHE40 and BHLHE41. Carey KL, Paulus GLC, Wang L, Balce DR, Luo JW, Bergman P, Ferder IC, Kong L, Renaud N, Singh S, Kost-Alimova M, Nyfeler B, Lassen KG, Virgin HW, Xavier RJ. Cell Rep. 2020 Nov 10;33(6):108371. doi: 10.1016/j.celrep.2020.108371. PubMed
Genome-Wide CRISPR-Cas9 Screen Reveals the Importance of the Heparan Sulfate Pathway and the Conserved Oligomeric Golgi Complex for Synthetic Double-Stranded RNA Uptake and Sindbis Virus Infection. Petitjean O, Girardi E, Ngondo RP, Lupashin V, Pfeffer S. mSphere. 2020 Nov 11;5(6). pii: 5/6/e00914-20. doi: 10.1128/mSphere.00914-20. PubMed
Multi-omics integration identifies a selective vulnerability of colorectal cancer subtypes to YM155. Zhan T, Faehling V, Rauscher B, Betge J, Ebert MP, Boutros M. Int J Cancer. 2021 Apr 15;148(8):1948-1963. doi: 10.1002/ijc.33393. Epub 2020 Dec 4. PubMed
TRF2-mediated telomere protection is dispensable in pluripotent stem cells. Markiewicz-Potoczny M, Lobanova A, Loeb AM, Kirak O, Olbrich T, Ruiz S, Lazzerini Denchi E. Nature. 2021 Jan;589(7840):110-115. doi: 10.1038/s41586-020-2959-4. Epub 2020 Nov 25. PubMed
A Novel Nonsense GLI3 Variant Is Associated With Polydactyly and Syndactyly in a Family by Blocking the Sonic Hedgehog Signaling Pathway. Xiang Y, Li X, Zhan Z, Feng J, Cai H, Li Y, Fu Q, Xu Y, Jiang H, Zhang X. Front Genet. 2020 Nov 10;11:542004. doi: 10.3389/fgene.2020.542004. eCollection 2020. PubMed
Genetic Screens Identify Host Factors for SARS-CoV-2 and Common Cold Coronaviruses. Wang R, Simoneau CR, Kulsuptrakul J, Bouhaddou M, Travisano KA, Hayashi JM, Carlson-Stevermer J, Zengel JR, Richards CM, Fozouni P, Oki J, Rodriguez L, Joehnk B, Walcott K, Holden K, Sil A, Carette JE, Krogan NJ, Ott M, Puschnik AS. Cell. 2021 Jan 7;184(1):106-119.e14. doi: 10.1016/j.cell.2020.12.004. Epub 2020 Dec 9. PubMed
TMEM41B Is a Pan-flavivirus Host Factor. Hoffmann HH, Schneider WM, Rozen-Gagnon K, Miles LA, Schuster F, Razooky B, Jacobson E, Wu X, Yi S, Rudin CM, MacDonald MR, McMullan LK, Poirier JT, Rice CM. Cell. 2021 Jan 7;184(1):133-148.e20. doi: 10.1016/j.cell.2020.12.005. Epub 2020 Dec 9. PubMed
Hepatic Stellate Cells in Hepatocellular Carcinoma Promote Tumor Growth Via Growth Differentiation Factor 15 Production. Myojin Y, Hikita H, Sugiyama M, Sasaki Y, Fukumoto K, Sakane S, Makino Y, Takemura N, Yamada R, Shigekawa M, Kodama T, Sakamori R, Kobayashi S, Tatsumi T, Suemizu H, Eguchi H, Kokudo N, Mizokami M, Takehara T. Gastroenterology. 2021 Apr;160(5):1741-1754.e16. doi: 10.1053/j.gastro.2020.12.015. Epub 2020 Dec 17. PubMed
Massively parallel functional testing of MSH2 missense variants conferring Lynch syndrome risk. Jia X, Burugula BB, Chen V, Lemons RM, Jayakody S, Maksutova M, Kitzman JO. Am J Hum Genet. 2021 Jan 7;108(1):163-175. doi: 10.1016/j.ajhg.2020.12.003. Epub 2020 Dec 23. PubMed
TMEM70 forms oligomeric scaffolds within mitochondrial cristae promoting in situ assembly of mammalian ATP synthase proton channel. Bahri H, Buratto J, Rojo M, Dompierre JP, Salin B, Blancard C, Cuvellier S, Rose M, Ben Ammar Elgaaied A, Tetaud E, di Rago JP, Devin A, Duvezin-Caubet S. Biochim Biophys Acta Mol Cell Res. 2021 Apr;1868(4):118942. doi: 10.1016/j.bbamcr.2020.118942. Epub 2020 Dec 23. PubMed
Genome-wide CRISPR screen identifies regulators of MAPK and MTOR pathways mediating sorafenib resistance in acute myeloid leukemia. Damnernsawad A, Bottomly D, Kurtz SE, Eide CA, McWeeney SK, Tyner JW, Nechiporuk T. Haematologica. 2020 Dec 30;Online ahead of print. doi: 10.3324/haematol.2020.257964. PubMed
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Functional Genomics Identify Distinct and Overlapping Genes Mediating Resistance to Different Classes of Heterobifunctional Degraders of Oncoproteins. Shirasaki R, Matthews GM, Gandolfi S, de Matos Simoes R, Buckley DL, Raja Vora J, Sievers QL, Bruggenthies JB, Dashevsky O, Poarch H, Tang H, Bariteau MA, Sheffer M, Hu Y, Downey-Kopyscinski SL, Hengeveld PJ, Glassner BJ, Dhimolea E, Ott CJ, Zhang T, Kwiatkowski NP, Laubach JP, Schlossman RL, Richardson PG, Culhane AC, Groen RWJ, Fischer ES, Vazquez F, Tsherniak A, Hahn WC, Levy J, Auclair D, Licht JD, Keats JJ, Boise LH, Ebert BL, Bradner JE, Gray NS, Mitsiades CS. Cell Rep. 2021 Jan 5;34(1):108532. doi: 10.1016/j.celrep.2020.108532. PubMed
Multiplex gene targeting in the mouse embryo using a Cas9-Cpf1 hybrid guide RNA. Oh SH, Lee HJ, Ahn MK, Jeon MY, Yoon JS, Jung YJ, Kim GN, Baek IJ, Kim I, Kim KM, Sung YH. Biochem Biophys Res Commun. 2021 Feb 5;539:48-55. doi: 10.1016/j.bbrc.2020.12.072. Epub 2021 Jan 6. PubMed
Direct Intracellular Visualization of Ebola Virus-Receptor Interaction by In Situ Proximity Ligation. Mittler E, Alkutkar T, Jangra RK, Chandran K. mBio. 2021 Jan 12;12(1). pii: mBio.03100-20. doi: 10.1128/mBio.03100-20. PubMed
Targeting Notch and EGFR signaling in human mucoepidermoid carcinoma. Ni W, Chen Z, Zhou X, Yang R, Yu M, Lu J, Kaye FJ, Wu L. Signal Transduct Target Ther. 2021 Jan 21;6(1):27. doi: 10.1038/s41392-020-00388-0. PubMed
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A host receptor enables type 1 pilus-mediated pathogenesis of Escherichia coli pyelonephritis. McLellan LK, McAllaster MR, Kim AS, Tothova L, Olson PD, Pinkner JS, Daugherty AL, Hreha TN, Janetka JW, Fremont DH, Hultgren SJ, Virgin HW, Hunstad DA. PLoS Pathog. 2021 Jan 29;17(1):e1009314. doi: 10.1371/journal.ppat.1009314. eCollection 2021 Jan. PubMed
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Targeted brachyury degradation disrupts a highly specific autoregulatory program controlling chordoma cell identity. Sheppard HE, Dall'Agnese A, Park WD, Shamim MH, Dubrulle J, Johnson HL, Stossi F, Cogswell P, Sommer J, Levy J, Sharifnia T, Wawer MJ, Nabet B, Gray NS, Clemons PA, Schreiber SL, Workman P, Young RA, Lin CY. Cell Rep Med. 2021 Jan 19;2(1):100188. doi: 10.1016/j.xcrm.2020.100188. eCollection 2021 Jan 19. PubMed Associated Plasmids Phage-PGK-Flag-HA-FKBP-T(WT) Phage-PGK-Flag-HA-FKBP-T(G177D)
The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. Phoomak C, Cui W, Hayman TJ, Yu SH, Zhao P, Wells L, Steet R, Contessa JN. Sci Adv. 2021 Jan 15;7(3). pii: 7/3/eabc6364. doi: 10.1126/sciadv.abc6364. Print 2021 Jan. PubMed
N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells. Song N, Chen L, Ren X, Waterfield NR, Yang J, Yang G. PLoS Pathog. 2021 Feb 4;17(2):e1009244. doi: 10.1371/journal.ppat.1009244. eCollection 2021 Feb. PubMed
Enhancing CRISPR deletion via pharmacological delay of DNA-PKcs. Bosch-Guiteras N, Uroda T, Guillen-Ramirez HA, Riedo R, Gazdhar A, Esposito R, Pulido-Quetglas C, Zimmer Y, Medova M, Johnson R. Genome Res. 2021 Mar;31(3):461-471. doi: 10.1101/gr.265736.120. Epub 2021 Feb 11. PubMed
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Efficient Immune Cell Genome Engineering with Enhanced CRISPR Editing Tools. Chan W, Gottschalk RA, Yao Y, Pomerantz JL, Germain RN. Immunohorizons. 2021 Feb 23;5(2):117-132. doi: 10.4049/immunohorizons.2000082. PubMed Associated Plasmids pCW-Cas9-2A-EGFP LentiGuide-TagBFP-2A-BSD LentiGuide-TagRFP-2A-BSD LentiCRISPRv2R LES2A-CRE More ...
Identification of gene targets of mutant C/EBPalpha reveals a critical role for MSI2 in CEBPA-mutated AML. Heyes E, Schmidt L, Manhart G, Eder T, Proietti L, Grebien F. Leukemia. 2021 Sep;35(9):2526-2538. doi: 10.1038/s41375-021-01169-6. Epub 2021 Feb 23. PubMed
Susceptibility-Associated Genetic Variation in NEDD9 Contributes to Prostate Cancer Initiation and Progression. Han D, Owiredu JN, Healy BM, Li M, Labaf M, Steinfeld JS, Patalano S, Gao S, Liu M, Macoska JA, Zarringhalam K, Siegfried KR, Yuan X, Rebbeck TR, Cai C. Cancer Res. 2021 Jul 15;81(14):3766-3776. doi: 10.1158/0008-5472.CAN-20-3042. Epub 2021 Feb 25. PubMed
Dissecting the role of TP53 alterations in del(11q) chronic lymphocytic leukemia. Quijada-Alamo M, Perez-Carretero C, Hernandez-Sanchez M, Rodriguez-Vicente AE, Herrero AB, Hernandez-Sanchez JM, Martin-Izquierdo M, Santos-Minguez S, Del Rey M, Gonzalez T, Rubio-Martinez A, Garcia de Coca A, Davila-Valls J, Hernandez-Rivas JA, Parker H, Strefford JC, Benito R, Ordonez JL, Hernandez-Rivas JM. Clin Transl Med. 2021 Feb;11(2):e304. doi: 10.1002/ctm2.304. PubMed
Transcription factor competition at the gamma-globin promoters controls hemoglobin switching. Liu N, Xu S, Yao Q, Zhu Q, Kai Y, Hsu JY, Sakon P, Pinello L, Yuan GC, Bauer DE, Orkin SH. Nat Genet. 2021 Apr;53(4):511-520. doi: 10.1038/s41588-021-00798-y. Epub 2021 Mar 1. PubMed
Complex-dependent histone acetyltransferase activity of KAT8 determines its role in transcription and cellular homeostasis. Radzisheuskaya A, Shliaha PV, Grinev VV, Shlyueva D, Damhofer H, Koche R, Gorshkov V, Kovalchuk S, Zhan Y, Rodriguez KL, Johnstone AL, Keogh MC, Hendrickson RC, Jensen ON, Helin K. Mol Cell. 2021 Apr 15;81(8):1749-1765.e8. doi: 10.1016/j.molcel.2021.02.012. Epub 2021 Mar 2. PubMed
Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane. Maruoka M, Zhang P, Mori H, Imanishi E, Packwood DM, Harada H, Kosako H, Suzuki J. Mol Cell. 2021 Apr 1;81(7):1397-1410.e9. doi: 10.1016/j.molcel.2021.02.025. Epub 2021 Mar 15. PubMed
O-GlcNAcylation homeostasis controlled by calcium influx channels regulates multiple myeloma dissemination. Samart P, Luanpitpong S, Rojanasakul Y, Issaragrisil S. J Exp Clin Cancer Res. 2021 Mar 16;40(1):100. doi: 10.1186/s13046-021-01876-z. PubMed
A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection. Kulsuptrakul J, Wang R, Meyers NL, Ott M, Puschnik AS. Cell Rep. 2021 Mar 16;34(11):108859. doi: 10.1016/j.celrep.2021.108859. PubMed
A hierarchical regulatory network analysis of the vitamin D induced transcriptome reveals novel regulators and complete VDR dependency in monocytes. Warwick T, Schulz MH, Gunther S, Gilsbach R, Neme A, Carlberg C, Brandes RP, Seuter S. Sci Rep. 2021 Mar 22;11(1):6518. doi: 10.1038/s41598-021-86032-5. PubMed
Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway. Fujino T, Goyama S, Sugiura Y, Inoue D, Asada S, Yamasaki S, Matsumoto A, Yamaguchi K, Isobe Y, Tsuchiya A, Shikata S, Sato N, Morinaga H, Fukuyama T, Tanaka Y, Fukushima T, Takeda R, Yamamoto K, Honda H, Nishimura EK, Furukawa Y, Shibata T, Abdel-Wahab O, Suematsu M, Kitamura T. Nat Commun. 2021 Mar 23;12(1):1826. doi: 10.1038/s41467-021-22053-y. PubMed
RRBP1 rewires cisplatin resistance in oral squamous cell carcinoma by regulating Hippo pathway. Shriwas O, Arya R, Mohanty S, Mohapatra P, Kumar S, Rath R, Kaushik SR, Pahwa F, Murmu KC, Majumdar SKD, Muduly DK, Dixit A, Prasad P, Nanda RK, Dash R. Br J Cancer. 2021 Jun;124(12):2004-2016. doi: 10.1038/s41416-021-01336-7. Epub 2021 Mar 24. PubMed
The mitochondrial Ca(2+) uptake regulator, MICU1, is involved in cold stress-induced ferroptosis. Nakamura T, Ogawa M, Kojima K, Takayanagi S, Ishihara S, Hattori K, Naguro I, Ichijo H. EMBO Rep. 2021 May 5;22(5):e51532. doi: 10.15252/embr.202051532. Epub 2021 Apr 6. PubMed
Development of p53 knockout U87MG cell line for unbiased drug delivery testing system using CRISPR-Cas9 and transcriptomic analysis. Kwon AT, Mohri K, Takizawa S, Arakawa T, Takahashi M, Kaczkowski B, Furuno M, Suzuki H, Tagami S, Mukai H, Arner E. J Biotechnol. 2021 May 20;332:72-82. doi: 10.1016/j.jbiotec.2021.03.017. Epub 2021 Apr 6. PubMed
Minor intron retention drives clonal hematopoietic disorders and diverse cancer predisposition. Inoue D, Polaski JT, Taylor J, Castel P, Chen S, Kobayashi S, Hogg SJ, Hayashi Y, Pineda JMB, El Marabti E, Erickson C, Knorr K, Fukumoto M, Yamazaki H, Tanaka A, Fukui C, Lu SX, Durham BH, Liu B, Wang E, Mehta S, Zakheim D, Garippa R, Penson A, Chew GL, McCormick F, Bradley RK, Abdel-Wahab O. Nat Genet. 2021 May;53(5):707-718. doi: 10.1038/s41588-021-00828-9. Epub 2021 Apr 12. PubMed
Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis. Su D, Wang W, Hou Y, Wang L, Yi X, Cao C, Wang Y, Gao H, Wang Y, Yang C, Liu B, Chen X, Wu X, Wu J, Yan D, Wei S, Han L, Liu S, Wang Q, Shi L, Shan L. Nucleic Acids Res. 2021 May 7;49(8):4421-4440. doi: 10.1093/nar/gkab209. PubMed
Inhibition of the FACT Complex Targets Aberrant Hedgehog Signaling and Overcomes Resistance to Smoothened Antagonists. Mo J, Liu F, Sun X, Huang H, Tan K, Zhao X, Li R, Jiang W, Sui Y, Chen X, Shen K, Zhang L, Ma J, Zhao K, Tang Y. Cancer Res. 2021 Jun 1;81(11):3105-3120. doi: 10.1158/0008-5472.CAN-20-3186. Epub 2021 Apr 14. PubMed
CRL4(AMBRA1) is a master regulator of D-type cyclins. Simoneschi D, Rona G, Zhou N, Jeong YT, Jiang S, Milletti G, Arbini AA, O'Sullivan A, Wang AA, Nithikasem S, Keegan S, Siu Y, Cianfanelli V, Maiani E, Nazio F, Cecconi F, Boccalatte F, Fenyo D, Jones DR, Busino L, Pagano M. Nature. 2021 Apr;592(7856):789-793. doi: 10.1038/s41586-021-03445-y. Epub 2021 Apr 14. PubMed Associated Plasmids pBABE-MCS(extended)-Puro (empty vector) pcDNA3.1-2xFLAG-2xSTREP (empty vector) pcDNA3.1-AMBRA1-3xFLAG px459-AMBRA1 #3 px458-AMBRA1 #3 More ...
The dystonia gene THAP1 controls DNA double-strand break repair choice. Shinoda K, Zong D, Callen E, Wu W, Dumitrache LC, Belinky F, Chari R, Wong N, Ishikawa M, Stanlie A, Multhaupt-Buell T, Sharma N, Ozelius L, Ehrlich M, McKinnon PJ, Nussenzweig A. Mol Cell. 2021 Jun 17;81(12):2611-2624.e10. doi: 10.1016/j.molcel.2021.03.034. Epub 2021 Apr 14. PubMed
ZNF410 represses fetal globin by singular control of CHD4. Vinjamur DS, Yao Q, Cole MA, McGuckin C, Ren C, Zeng J, Hossain M, Luk K, Wolfe SA, Pinello L, Bauer DE. Nat Genet. 2021 May;53(5):719-728. doi: 10.1038/s41588-021-00843-w. Epub 2021 Apr 15. PubMed
Identification of X-chromosomal genes that drive sex differences in embryonic stem cells through a hierarchical CRISPR screening approach. Genolet O, Monaco AA, Dunkel I, Boettcher M, Schulz EG. Genome Biol. 2021 Apr 16;22(1):110. doi: 10.1186/s13059-021-02321-2. PubMed
NSDHL promotes triple-negative breast cancer metastasis through the TGFbeta signaling pathway and cholesterol biosynthesis. Chen M, Zhao Y, Yang X, Zhao Y, Liu Q, Liu Y, Hou Y, Sun H, Jin W. Breast Cancer Res Treat. 2021 Jun;187(2):349-362. doi: 10.1007/s10549-021-06213-8. Epub 2021 Apr 16. PubMed
STING enhances cell death through regulation of reactive oxygen species and DNA damage. Hayman TJ, Baro M, MacNeil T, Phoomak C, Aung TN, Cui W, Leach K, Iyer R, Challa S, Sandoval-Schaefer T, Burtness BA, Rimm DL, Contessa JN. Nat Commun. 2021 Apr 19;12(1):2327. doi: 10.1038/s41467-021-22572-8. PubMed
Profiling the genetic determinants of chromatin accessibility with scalable single-cell CRISPR screens. Liscovitch-Brauer N, Montalbano A, Deng J, Mendez-Mancilla A, Wessels HH, Moss NG, Kung CY, Sookdeo A, Guo X, Geller E, Jaini S, Smibert P, Sanjana NE. Nat Biotechnol. 2021 Apr 29. pii: 10.1038/s41587-021-00902-x. doi: 10.1038/s41587-021-00902-x. PubMed Associated Plasmids pTXB1-TnY
The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains. Zhemkov V, Ditlev JA, Lee WR, Wilson M, Liou J, Rosen MK, Bezprozvanny I. Elife. 2021 May 11;10. pii: 65192. doi: 10.7554/eLife.65192. PubMed
ArfGAP1 inhibits mTORC1 lysosomal localization and activation. Meng D, Yang Q, Melick CH, Park BC, Hsieh TS, Curukovic A, Jeong MH, Zhang J, James NG, Jewell JL. EMBO J. 2021 Jun 15;40(12):e106412. doi: 10.15252/embj.2020106412. Epub 2021 May 14. PubMed
Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway. Ganga AK, Kennedy MC, Oguchi ME, Gray S, Oliver KE, Knight TA, De La Cruz EM, Homma Y, Fukuda M, Breslow DK. Curr Biol. 2021 Jul 12;31(13):2895-2905.e7. doi: 10.1016/j.cub.2021.04.075. Epub 2021 May 13. PubMed
A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture. Zhao Y, Jiang S, Zhang J, Guan XL, Sun BG, Sun L. Virulence. 2021 Dec;12(1):1362-1376. doi: 10.1080/21505594.2021.1926649. PubMed
PKD2 deficiency suppresses amino acid biosynthesis in ADPKD by impairing the PERK-TBL2-eIF2a-ATF4 pathway. Zhou X, Xiong H, Lu Y, Geng M, Lu Y, Zhang L, Zhu X. Biochem Biophys Res Commun. 2021 Jul 5;561:73-79. doi: 10.1016/j.bbrc.2021.05.012. Epub 2021 May 18. PubMed
Oncogenic KRAS engages an RSK1/NF1 pathway to inhibit wild-type RAS signaling in pancreatic cancer. Cheng DK, Oni TE, Thalappillil JS, Park Y, Ting HC, Alagesan B, Prasad NV, Addison K, Rivera KD, Pappin DJ, Van Aelst L, Tuveson DA. Proc Natl Acad Sci U S A. 2021 May 25;118(21). pii: 2016904118. doi: 10.1073/pnas.2016904118. PubMed
MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells. Muller I, Moroni AS, Shlyueva D, Sahadevan S, Schoof EM, Radzisheuskaya A, Hojfeldt JW, Tatar T, Koche RP, Huang C, Helin K. Nat Commun. 2021 May 24;12(1):3034. doi: 10.1038/s41467-021-23308-4. PubMed Associated Plasmids pRepair-MPP8_mAID-P2A-BFP pL-U6-sgRNA-SFFV-Puro-P2A-EGFP pPB-MPP8_Flag2-Blast pPB-MPP8-112–858_Flag2-Blast pPB-MPP8-1–729_Flag2-Blast More ...
Dysregulation of PI3K and Hippo signaling pathways synergistically induces chronic pancreatitis via CTGF upregulation. Tamura T, Kodama T, Sato K, Murai K, Yoshioka T, Shigekawa M, Yamada R, Hikita H, Sakamori R, Akita H, Eguchi H, Johnson RL, Yokoi H, Mukoyama M, Tatsumi T, Takehara T. J Clin Invest. 2021 Jul 1;131(13). pii: 143414. doi: 10.1172/JCI143414. PubMed
LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components. Fan Y, Koberlin MS, Ratnayeke N, Liu C, Deshpande M, Gerhardt J, Meyer T. J Cell Biol. 2021 Aug 2;220(8). pii: 212186. doi: 10.1083/jcb.202009147. Epub 2021 May 26. PubMed
TMEM41B is a host factor required for the replication of diverse coronaviruses including SARS-CoV-2. Trimarco JD, Heaton BE, Chaparian RR, Burke KN, Binder RA, Gray GC, Smith CM, Menachery VD, Heaton NS. PLoS Pathog. 2021 May 27;17(5):e1009599. doi: 10.1371/journal.ppat.1009599. eCollection 2021 May. PubMed
Pooled CRISPR screening in pancreatic cancer cells implicates co-repressor complexes as a cause of multiple drug resistance via regulation of epithelial-to-mesenchymal transition. Ramaker RC, Hardigan AA, Gordon ER, Wright CA, Myers RM, Cooper SJ. BMC Cancer. 2021 May 29;21(1):632. doi: 10.1186/s12885-021-08388-1. PubMed
Ultra-high-throughput single-cell RNA sequencing and perturbation screening with combinatorial fluidic indexing. Datlinger P, Rendeiro AF, Boenke T, Senekowitsch M, Krausgruber T, Barreca D, Bock C. Nat Methods. 2021 Jun;18(6):635-642. doi: 10.1038/s41592-021-01153-z. Epub 2021 May 31. PubMed
A BMPR2/YY1 Signaling Axis Is Required for Human Cytomegalovirus Latency in Undifferentiated Myeloid Cells. Poole E, Carlan da Silva MC, Huang C, Perera M, Jackson S, Groves IJ, Wills M, Rana A, Sinclair J. mBio. 2021 Jun 29;12(3):e0022721. doi: 10.1128/mBio.00227-21. Epub 2021 Jun 1. PubMed
A cell-free assay implicates a role of sphingomyelin and cholesterol in STING phosphorylation. Takahashi K, Niki T, Ogawa E, Fumika K, Nishioka Y, Sawa M, Arai H, Mukai K, Taguchi T. Sci Rep. 2021 Jun 7;11(1):11996. doi: 10.1038/s41598-021-91562-z. PubMed
HGK promotes metastatic dissemination in prostate cancer. Garcia-Garcia S, Rodrigo-Faus M, Fonseca N, Manzano S, Gyorffy B, Ocana A, Bragado P, Porras A, Gutierrez-Uzquiza A. Sci Rep. 2021 Jun 10;11(1):12287. doi: 10.1038/s41598-021-91292-2. PubMed
Inactivation of Latent HIV-1 Proviral DNA Using Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Treatment and the Assessment of Off-Target Effects. Xu Y, Peng X, Zheng Y, Jin C, Lu X, Han D, Fu H, Chen C, Wu N. Front Microbiol. 2021 May 26;12:629153. doi: 10.3389/fmicb.2021.629153. eCollection 2021. PubMed
Genome-scale CRISPR-Cas9 screen of Wnt/beta-catenin signaling identifies therapeutic targets for colorectal cancer. Wan C, Mahara S, Sun C, Doan A, Chua HK, Xu D, Bian J, Li Y, Zhu D, Sooraj D, Cierpicki T, Grembecka J, Firestein R. Sci Adv. 2021 May 19;7(21). pii: 7/21/eabf2567. doi: 10.1126/sciadv.abf2567. Print 2021 May. PubMed
Dependency of human and murine LKB1-inactivated lung cancer on aberrant CRTC-CREB activation. Zhou X, Li JW, Chen Z, Ni W, Li X, Yang R, Shen H, Liu J, DeMayo FJ, Lu J, Kaye FJ, Wu L. Elife. 2021 Jun 18;10. pii: 66095. doi: 10.7554/eLife.66095. PubMed
Pharmacologic modulation of RNA splicing enhances anti-tumor immunity. Lu SX, De Neef E, Thomas JD, Sabio E, Rousseau B, Gigoux M, Knorr DA, Greenbaum B, Elhanati Y, Hogg SJ, Chow A, Ghosh A, Xie A, Zamarin D, Cui D, Erickson C, Singer M, Cho H, Wang E, Lu B, Durham BH, Shah H, Chowell D, Gabel AM, Shen Y, Liu J, Jin J, Rhodes MC, Taylor RE, Molina H, Wolchok JD, Merghoub T, Diaz LA Jr, Abdel-Wahab O, Bradley RK. Cell. 2021 Jul 22;184(15):4032-4047.e31. doi: 10.1016/j.cell.2021.05.038. Epub 2021 Jun 24. PubMed
A first-in-class Polymerase Theta Inhibitor selectively targets Homologous-Recombination-Deficient Tumors. Zhou J, Gelot C, Pantelidou C, Li A, Yucel H, Davis RE, Farkkila A, Kochupurakkal B, Syed A, Shapiro GI, Tainer JA, Blagg BSJ, Ceccaldi R, D'Andrea AD. Nat Cancer. 2021 Jun;2(6):598-610. doi: 10.1038/s43018-021-00203-x. Epub 2021 Jun 17. PubMed
BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation. Conway E, Rossi F, Fernandez-Perez D, Ponzo E, Ferrari KJ, Zanotti M, Manganaro D, Rodighiero S, Tamburri S, Pasini D. Mol Cell. 2021 Sep 2;81(17):3526-3541.e8. doi: 10.1016/j.molcel.2021.06.020. Epub 2021 Jun 28. PubMed
The Protein Landscape of Chronic Lymphocytic Leukemia (CLL). Meier-Abt F, Lu J, Cannizzaro E, Pohly MF, Kummer S, Pfammatter S, Kunz L, Collins BC, Nadeu F, Lee KS, Xue P, Gwerder M, Roiss M, Hullein J, Scheinost S, Dietrich S, Campo E, Huber W, Aebersold R, Zenz T. Blood. 2021 Jun 29. pii: S0006-4971(21)01293-3. doi: 10.1182/blood.2020009741. PubMed
Central Nervous System-Infecting Pathogens Escherichia coli and Cryptococcus neoformans Exploit the Host Pdlim2 for Intracellular Traversal and Exocytosis in the Blood-Brain Barrier. Li Z, Bruno VM, Kim KS. Infect Immun. 2021 Sep 16;89(10):e0012821. doi: 10.1128/IAI.00128-21. Epub 2021 Jul 6. PubMed
HNRNPL Circularizes ARHGAP35 to Produce an Oncogenic Protein. Li Y, Chen B, Zhao J, Li Q, Chen S, Guo T, Li Y, Lai H, Chen Z, Meng Z, Guo W, He X, Huang S. Adv Sci (Weinh). 2021 May 1;8(13):2001701. doi: 10.1002/advs.202001701. eCollection 2021 Jul. PubMed
HLA class-I-peptide stability mediates CD8(+) T cell immunodominance hierarchies and facilitates HLA-associated immune control of HIV. Kaseke C, Park RJ, Singh NK, Koundakjian D, Bashirova A, Garcia Beltran WF, Takou Mbah OC, Ma J, Senjobe F, Urbach JM, Nathan A, Rossin EJ, Tano-Menka R, Khatri A, Piechocka-Trocha A, Waring MT, Birnbaum ME, Baker BM, Carrington M, Walker BD, Gaiha GD. Cell Rep. 2021 Jul 13;36(2):109378. doi: 10.1016/j.celrep.2021.109378. PubMed
Human cell transformation by combined lineage conversion and oncogene expression. Sahu B, Pihlajamaa P, Zhang K, Palin K, Ahonen S, Cervera A, Ristimaki A, Aaltonen LA, Hautaniemi S, Taipale J. Oncogene. 2021 Sep;40(36):5533-5547. doi: 10.1038/s41388-021-01940-0. Epub 2021 Jul 23. PubMed
Cancer LncRNA Census 2 (CLC2): an enhanced resource reveals clinical features of cancer lncRNAs. Vancura A, Lanzos A, Bosch-Guiteras N, Esteban MT, Gutierrez AH, Haefliger S, Johnson R. NAR Cancer. 2021 Apr 14;3(2):zcab013. doi: 10.1093/narcan/zcab013. eCollection 2021 Jun. PubMed
Metabolically controlled histone H4K5 acylation/acetylation ratio drives BRD4 genomic distribution. Gao M, Wang J, Rousseaux S, Tan M, Pan L, Peng L, Wang S, Xu W, Ren J, Liu Y, Spinck M, Barral S, Wang T, Chuffart F, Bourova-Flin E, Puthier D, Curtet S, Bargier L, Cheng Z, Neumann H, Li J, Zhao Y, Mi JQ, Khochbin S. Cell Rep. 2021 Jul 27;36(4):109460. doi: 10.1016/j.celrep.2021.109460. PubMed
MYC suppresses STING-dependent innate immunity by transcriptionally upregulating DNMT1 in triple-negative breast cancer. Wu SY, Xiao Y, Wei JL, Xu XE, Jin X, Hu X, Li DQ, Jiang YZ, Shao ZM. J Immunother Cancer. 2021 Jul;9(7). pii: jitc-2021-002528. doi: 10.1136/jitc-2021-002528. PubMed
PALI1 facilitates DNA and nucleosome binding by PRC2 and triggers an allosteric activation of catalysis. Zhang Q, Agius SC, Flanigan SF, Uckelmann M, Levina V, Owen BM, Davidovich C. Nat Commun. 2021 Jul 28;12(1):4592. doi: 10.1038/s41467-021-24866-3. PubMed Associated Plasmids PALI1_PIR_pFB1 ATOH1_pUC18 LacZ_pHIVEGFP PALI1_pHIVEGFP
Endophilin A2 regulates B-cell endocytosis and is required for germinal center and humoral responses. Malinova D, Wasim L, Newman R, Martinez-Riano A, Engels N, Tolar P. EMBO Rep. 2021 Sep 6;22(9):e51328. doi: 10.15252/embr.202051328. Epub 2021 Jul 29. PubMed
LKB1 inactivation modulates chromatin accessibility to drive metastatic progression. Pierce SE, Granja JM, Corces MR, Brady JJ, Tsai MK, Pierce AB, Tang R, Chu P, Feldser DM, Chang HY, Bassik MC, Greenleaf WJ, Winslow MM. Nat Cell Biol. 2021 Aug;23(8):915-924. doi: 10.1038/s41556-021-00728-4. Epub 2021 Aug 2. PubMed
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Intrinsic activation of beta-catenin signaling by CRISPR/Cas9-mediated exon skipping contributes to immune evasion in hepatocellular carcinoma. Akasu M, Shimada S, Kabashima A, Akiyama Y, Shimokawa M, Akahoshi K, Kudo A, Yamaoka S, Tanabe M, Tanaka S. Sci Rep. 2021 Aug 24;11(1):16732. doi: 10.1038/s41598-021-96167-0. PubMed
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SMARCA4/2 loss inhibits chemotherapy-induced apoptosis by restricting IP3R3-mediated Ca(2+) flux to mitochondria. Xue Y, Morris JL, Yang K, Fu Z, Zhu X, Johnson F, Meehan B, Witkowski L, Yasmeen A, Golenar T, Coatham M, Morin G, Monast A, Pilon V, Fiset PO, Jung S, Gonzalez AV, Camilleri-Broet S, Fu L, Postovit LM, Spicer J, Gotlieb WH, Guiot MC, Rak J, Park M, Lockwood W, Foulkes WD, Prudent J, Huang S. Nat Commun. 2021 Sep 13;12(1):5404. doi: 10.1038/s41467-021-25260-9. PubMed
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PLCG1 is required for AML1-ETO leukemia stem cell self-renewal. Schnoeder TM, Schwarzer A, Jayavelu AK, Hsu CJ, Kirkpatrick J, Dohner K, Perner F, Eifert T, Huber N, Arreba-Tutusaus P, Dolnik A, Assi SA, Nafria M, Jiang L, Dai YT, Chen Z, Chen SJ, Kellaway SG, Ptasinska A, Ng ES, Stanley EG, Elefanty AG, Buschbeck M, Bierhoff H, Brodt S, Matziolis G, Fischer KD, Hochhaus A, Chen CW, Heidenreich O, Mann M, Lane SW, Bullinger L, Ori A, von Eyss B, Bonifer C, Heidel FH. Blood. 2022 Feb 17;139(7):1080-1097. doi: 10.1182/blood.2021012778. PubMed
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The chemotherapeutic CX-5461 primarily targets TOP2B and exhibits selective activity in high-risk neuroblastoma. Pan M, Wright WC, Chapple RH, Zubair A, Sandhu M, Batchelder JE, Huddle BC, Low J, Blankenship KB, Wang Y, Gordon B, Archer P, Brady SW, Natarajan S, Posgai MJ, Schuetz J, Miller D, Kalathur R, Chen S, Connelly JP, Babu MM, Dyer MA, Pruett-Miller SM, Freeman BB 3rd, Chen T, Godley LA, Blanchard SC, Stewart E, Easton J, Geeleher P. Nat Commun. 2021 Nov 9;12(1):6468. doi: 10.1038/s41467-021-26640-x. PubMed
Self-guarding of MORC3 enables virulence factor-triggered immunity. Gaidt MM, Morrow A, Fairgrieve MR, Karr JP, Yosef N, Vance RE. Nature. 2021 Dec;600(7887):138-142. doi: 10.1038/s41586-021-04054-5. Epub 2021 Nov 10. PubMed
The specificity of the malarial VAR2CSA protein for chondroitin sulfate depends on 4-O-sulfation and ligand accessibility. Spliid CB, Toledo AG, Sanderson P, Mao Y, Gatto F, Gustavsson T, Choudhary S, Saldanha AL, Vogelsang RP, Gogenur I, Theander TG, Leach FE 3rd, Amster IJ, Esko JD, Salanti A, Clausen TM. J Biol Chem. 2021 Dec;297(6):101391. doi: 10.1016/j.jbc.2021.101391. Epub 2021 Nov 8. PubMed
LIN28B alters ribosomal dynamics to promote metastasis in MYCN-driven malignancy. Missios P, da Rocha EL, Pearson DS, Philipp J, Aleman MM, Pirouz M, Farache D, Franses JW, Kubaczka C, Tsanov KM, Jha DK, Pepe-Mooney B, Powers JT, Gregory RI, Lee AS, Dominguez D, Ting DT, Daley GQ. J Clin Invest. 2021 Nov 15;131(22). pii: 145142. doi: 10.1172/JCI145142. PubMed
Genome-scale CRISPR-Cas9 knockout screening in hepatocellular carcinoma with lenvatinib resistance. Lu Y, Shen H, Huang W, He S, Chen J, Zhang D, Shen Y, Sun Y. Cell Death Discov. 2021 Nov 18;7(1):359. doi: 10.1038/s41420-021-00747-y. PubMed
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FAM72A antagonizes UNG2 to promote mutagenic repair during antibody maturation. Feng Y, Li C, Stewart JA, Barbulescu P, Seija Desivo N, Alvarez-Quilon A, Pezo RC, Perera MLW, Chan K, Tong AHY, Mohamad-Ramshan R, Berru M, Nakib D, Li G, Kardar GA, Carlyle JR, Moffat J, Durocher D, Di Noia JM, Bhagwat AS, Martin A. Nature. 2021 Dec;600(7888):324-328. doi: 10.1038/s41586-021-04144-4. Epub 2021 Nov 24. PubMed
The SETDB1-TRIM28 Complex Suppresses Antitumor Immunity. Lin J, Guo D, Liu H, Zhou W, Wang C, Muller I, Kossenkov AV, Drapkin R, Bitler BG, Helin K, Zhang R. Cancer Immunol Res. 2021 Dec;9(12):1413-1424. doi: 10.1158/2326-6066.CIR-21-0754. PubMed
KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs. Yan F, Li J, Milosevic J, Petroni R, Liu S, Shi Z, Yuan S, Reynaga JM, Qi Y, Rico J, Yu S, Liu Y, Rokudai S, Palmisiano N, Meyer SE, Sung PJ, Wan L, Lan F, Garcia BA, Stanger BZ, Sykes DB, Blanco MA. Cancer Discov. 2022 Mar 1;12(3):792-811. doi: 10.1158/2159-8290.CD-20-1459. PubMed
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Lysoptosis is an evolutionarily conserved cell death pathway moderated by intracellular serpins. Luke CJ, Markovina S, Good M, Wight IE, Thomas BJ, Linneman JM, Lanik WE, Koroleva O, Coffman MR, Miedel MT, Gong Q, Andress A, Campos Guerrero M, Wang S, Chen L, Beatty WL, Hausmann KN, White FV, Fitzpatrick JAJ, Orvedahl A, Pak SC, Silverman GA. Commun Biol. 2022 Jan 12;5(1):47. doi: 10.1038/s42003-021-02953-x. PubMed
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A Two-tiered functional screen identifies herpesviral transcriptional modifiers and their essential domains. Morgens DW, Nandakumar D, Didychuk AL, Yang KJ, Glaunsinger BA. PLoS Pathog. 2022 Jan 18;18(1):e1010236. doi: 10.1371/journal.ppat.1010236. eCollection 2022 Jan. PubMed Associated Plasmids KSHV Tiling Library
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CRISPR and biochemical screens identify MAZ as a cofactor in CTCF-mediated insulation at Hox clusters. Ortabozkoyun H, Huang PY, Cho H, Narendra V, LeRoy G, Gonzalez-Buendia E, Skok JA, Tsirigos A, Mazzoni EO, Reinberg D. Nat Genet. 2022 Feb;54(2):202-212. doi: 10.1038/s41588-021-01008-5. Epub 2022 Feb 10. PubMed
Base editing sensor libraries for high-throughput engineering and functional analysis of cancer-associated single nucleotide variants. Sanchez-Rivera FJ, Diaz BJ, Kastenhuber ER, Schmidt H, Katti A, Kennedy M, Tem V, Ho YJ, Leibold J, Paffenholz SV, Barriga FM, Chu K, Goswami S, Wuest AN, Simon JM, Tsanov KM, Chakravarty D, Zhang H, Leslie CS, Lowe SW, Dow LE. Nat Biotechnol. 2022 Feb 14. pii: 10.1038/s41587-021-01172-3. doi: 10.1038/s41587-021-01172-3. PubMed Associated Plasmids Mouse Base Editing Sensor Library Human Base Editing Sensor Library ALL-PAM sensor Library
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Coagulation factors directly cleave SARS-CoV-2 spike and enhance viral entry. Kastenhuber ER, Mercadante M, Nilsson-Payant B, Johnson JL, Jaimes JA, Muecksch F, Weisblum Y, Bram Y, Chandar V, Whittaker GR, tenOever BR, Schwartz RE, Cantley L. Elife. 2022 Mar 23;11. pii: 77444. doi: 10.7554/eLife.77444. PubMed Associated Plasmids pEGPN pEGPN-ACE2 pEGPN-TMPRSS2
Bcl-xL Enforces a Slow-Cycling State Necessary for Survival in the Nutrient-Deprived Microenvironment of Pancreatic Cancer. Sela Y, Li J, Maheswaran S, Norgard R, Yuan S, Hubbi M, Doepner M, Xu JP, Ho ES, Mesaros C, Sheehan C, Croley G, Muir A, Blair IA, Shalem O, Dang CV, Stanger BZ. Cancer Res. 2022 May 16;82(10):1890-1908. doi: 10.1158/0008-5472.CAN-22-0431. PubMed
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Candida albicans stimulates the formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection. Phan QT, Solis NV, Cravener MV, Swidergall M, Lin J, Huang MY, Liu H, Singh S, Ibrahim AS, Mazzone M, Mitchell AP, Filler SG. bioRxiv. 2023 Feb 23:2023.02.23.529756. doi: 10.1101/2023.02.23.529756. Preprint. PubMed
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In Vitro Generation of Murine Dendritic Cells from Hoxb8-Immortalized Hematopoietic Progenitors. Hacker H. Methods Mol Biol. 2023;2618:93-107. doi: 10.1007/978-1-0716-2938-3_7. PubMed
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The protective role of conjunctival goblet cell mucin sialylation. Matsuzawa M, Ando T, Fukase S, Kimura M, Kume Y, Ide T, Izawa K, Kaitani A, Hara M, Nakamura E, Kamei A, Matsuda A, Nakano N, Maeda K, Tada N, Ogawa H, Okumura K, Murakami A, Ebihara N, Kitaura J. Nat Commun. 2023 Mar 17;14(1):1417. doi: 10.1038/s41467-023-37101-y. PubMed
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Long noncoding RNA Malat1 inhibits Tead3-Nfatc1-mediated osteoclastogenesis to suppress osteoporosis and bone metastasis. Zhao Y, Teng H, Deng Y, Sheldon M, Martinez C, Zhang J, Tian A, Sun Y, Nakagawa S, Yao F, Wang H, Ma L. Res Sq. 2023 Mar 20:rs.3.rs-2405644. doi: 10.21203/rs.3.rs-2405644/v1. Preprint. PubMed
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Cytotoxicity of CD19-CAR-NK92 cells is primarily mediated via perforin/granzyme pathway. Althaus J, Nilius-Eliliwi V, Maghnouj A, Doring S, Schroers R, Hudecek M, Hahn SA, Mika T. Cancer Immunol Immunother. 2023 Aug;72(8):2573-2583. doi: 10.1007/s00262-023-03443-1. Epub 2023 Apr 13. PubMed
An In Vivo CRISPR Screen Identifies That SNRPC Promotes Triple-Negative Breast Cancer Progression. Lu XX, Yang WX, Pei YC, Luo H, Li XG, Wang YJ, Zhang GL, Ling H, Shao ZM, Hu X. Cancer Res. 2023 Jun 15;83(12):2000-2015. doi: 10.1158/0008-5472.CAN-22-0536. PubMed
Mannose metabolism inhibition sensitizes acute myeloid leukaemia cells to therapy by driving ferroptotic cell death. Woodley K, Dillingh LS, Giotopoulos G, Madrigal P, Rattigan KM, Philippe C, Dembitz V, Magee AMS, Asby R, van de Lagemaat LN, Mapperley C, James SC, Prehn JHM, Tzelepis K, Rouault-Pierre K, Vassiliou GS, Kranc KR, Helgason GV, Huntly BJP, Gallipoli P. Nat Commun. 2023 Apr 14;14(1):2132. doi: 10.1038/s41467-023-37652-0. PubMed
A multi-omics integrative analysis based on CRISPR screens re-defines the pluripotency regulatory network in ESCs. Ruan Y, Wang J, Yu M, Wang F, Wang J, Xu Y, Liu L, Cheng Y, Yang R, Zhang C, Yang Y, Wang J, Wu W, Huang Y, Tian Y, Chen G, Zhang J, Jian R. Commun Biol. 2023 Apr 14;6(1):410. doi: 10.1038/s42003-023-04700-w. PubMed
TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth. Basu A, Champagne RN, Patel NG, Nicholson ED, Weiss RJ. J Biol Chem. 2023 Jun;299(6):104713. doi: 10.1016/j.jbc.2023.104713. Epub 2023 Apr 13. PubMed
The viral packaging motor potentiates Kaposi's sarcoma-associated herpesvirus gene expression late in infection. McCollum CO, Didychuk AL, Liu D, Murray-Nerger LA, Cristea IM, Glaunsinger BA. PLoS Pathog. 2023 Apr 17;19(4):e1011163. doi: 10.1371/journal.ppat.1011163. eCollection 2023 Apr. PubMed Associated Plasmids pCDNA4.TO_ORF18_TurboID_3xHA pCDNA4.TO_ORF30_TurboID_3xHA pCDNA4.TO_ORF31_TurboID_3xHA pCDNA4.TO_ORF66_TurboID_3xHA pCDNA4.TO_3xHA_TurboID_ORF34 More ...
Epigenetic Control of Translation Checkpoint and Tumor Progression via RUVBL1-EEF1A1 Axis. Li M, Yang L, Chan AKN, Pokharel SP, Liu Q, Mattson N, Xu X, Chang WH, Miyashita K, Singh P, Zhang L, Li M, Wu J, Wang J, Chen B, Chan LN, Lee J, Zhang XH, Rosen ST, Muschen M, Qi J, Chen J, Hiom K, Bishop AJR, Chen CW. Adv Sci (Weinh). 2023 Jun;10(17):e2206584. doi: 10.1002/advs.202206584. Epub 2023 Apr 19. PubMed
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TRAF3 suppression encourages B cell recruitment and prolongs survival of microbiome-intact mice with ovarian cancer. Zorea J, Motro Y, Mazor RD, Carmi YK, Shulman Z, Mahajna J, Moran-Gilad J, Elkabets M. J Exp Clin Cancer Res. 2023 May 1;42(1):107. doi: 10.1186/s13046-023-02680-7. PubMed
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Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies. Guo L, Mao Q, He J, Liu X, Piao X, Luo L, Hao X, Yu H, Song Q, Xiao B, Fan D, Gao Z, Jia Y. Cell Res. 2023 Jul;33(7):497-515. doi: 10.1038/s41422-023-00798-z. Epub 2023 May 4. PubMed
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Parallel Genome-Wide CRISPR Screens to Identify State-Dependent Self-Renewal Regulators of Mouse Embryonic Stem Cells. Wang J, Zhang C, Huang Y, Ruan Y, Hu Y, Wang J, Wang F, Yu M, Xu Y, Liu L, Cheng Y, Yang R, Dong Y, Wang J, Yang Y, Xiong J, Tian Y, Gao Q, Zhang J, Jian R. Stem Cells Dev. 2023 Aug;32(15-16):450-464. doi: 10.1089/scd.2023.0053. Epub 2023 Jun 20. PubMed
Deep learning models to predict the editing efficiencies and outcomes of diverse base editors. Kim N, Choi S, Kim S, Song M, Seo JH, Min S, Park J, Cho SR, Kim HH. Nat Biotechnol. 2023 May 15. doi: 10.1038/s41587-023-01792-x. PubMed
Deep sampling of gRNA in the human genome and deep-learning-informed prediction of gRNA activities. Zhang H, Yan J, Lu Z, Zhou Y, Zhang Q, Cui T, Li Y, Chen H, Ma L. Cell Discov. 2023 May 16;9(1):48. doi: 10.1038/s41421-023-00549-9. PubMed
A histone deacetylase 3 and mitochondrial complex I axis regulates toxic formaldehyde production. Wit N, Gogola E, West JA, Vornbaumen T, Seear RV, Bailey PSJ, Burgos-Barragan G, Wang M, Krawczyk P, Huberts DHEW, Gergely F, Matheson NJ, Kaser A, Nathan JA, Patel KJ. Sci Adv. 2023 May 19;9(20):eadg2235. doi: 10.1126/sciadv.adg2235. Epub 2023 May 17. PubMed
Prolactin receptor signaling induces acquisition of chemoresistance and reduces clonogenicity in acute myeloid leukemia. Cuesta-Casanovas L, Delgado-Martinez J, Cornet-Masana JM, Carbo JM, Banus-Mulet A, Guijarro F, Esteve J, Risueno RM. Cancer Cell Int. 2023 May 19;23(1):97. doi: 10.1186/s12935-023-02944-4. PubMed
Studying TIGIT activity against tumors through the generation of knockout mice. Rishiq A, Bsoul R, Pick O, Mandelboim O. Oncoimmunology. 2023 May 29;12(1):2217735. doi: 10.1080/2162402X.2023.2217735. eCollection 2023. PubMed
Investigation of enzalutamide, docetaxel, and cabazitaxel resistance in the castration resistant prostate cancer cell line C4 using genome-wide CRISPR/Cas9 screening. Haldrup J, Weiss S, Schmidt L, Sorensen KD. Sci Rep. 2023 Jun 3;13(1):9043. doi: 10.1038/s41598-023-35950-7. PubMed
Host-specific sensing of coronaviruses and picornaviruses by the CARD8 inflammasome. Tsu BV, Agarwal R, Gokhale NS, Kulsuptrakul J, Ryan AP, Fay EJ, Castro LK, Beierschmitt C, Yap C, Turcotte EA, Delgado-Rodriguez SE, Vance RE, Hyde JL, Savan R, Mitchell PS, Daugherty MD. PLoS Biol. 2023 Jun 8;21(6):e3002144. doi: 10.1371/journal.pbio.3002144. eCollection 2023 Jun. PubMed
Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids. Huang B, Zeng Z, Li H, Li Z, Chen X, Guo J, Zhang CC, Schreiber ME, Vonk AC, Xiang T, Patel T, Li Y, Parvez RK, Der B, Chen JH, Liu Z, Thornton ME, Grubbs BH, Diao Y, Dou Y, Gnedeva K, Lindstrom NO, Ying Q, Pastor-Soler NM, Fei T, Hallows KR, McMahon AP, Li Z. bioRxiv. 2023 May 25:2023.05.25.542343. doi: 10.1101/2023.05.25.542343. Preprint. PubMed
Loss of SFXN1 mitigates lipotoxicity and predicts poor outcome in non-viral hepatocellular carcinoma. Yagi K, Shimada S, Akiyama Y, Hatano M, Asano D, Ishikawa Y, Ueda H, Watanabe S, Akahoshi K, Ono H, Tanabe M, Tanaka S. Sci Rep. 2023 Jun 9;13(1):9449. doi: 10.1038/s41598-023-36660-w. PubMed
SMYD5 is a novel epigenetic gatekeeper of the mild hypothermia response. Rafnsdottir S, Jang K, Halldorsdottir ST, Tomasdottir A, Vinod M, Moller K, Reynisdottir T, Atladottir LH, Allison KE, He J, Zhang L, Northington FJ, Chavez-Valdez R, Anderson KJ, Bjornsson HT. bioRxiv. 2023 Jun 9:2023.05.11.540170. doi: 10.1101/2023.05.11.540170. Preprint. PubMed
STAT3-Mediated Promoter-Enhancer Interaction Up-Regulates Inhibitor of DNA Binding 1 (ID1) to Promote Colon Cancer Progression. Lin Z, Liu Y, Xu T, Su T, Yang Y, Liang R, Gu S, Li J, Song X, Liang B, Leng Z, Li Y, Meng L, Luo Y, Chang X, Huang D, Xie L. Int J Mol Sci. 2023 Jun 12;24(12):10041. doi: 10.3390/ijms241210041. PubMed
Impact of cell cycle on repair of ruptured nuclear envelope and sensitivity to nuclear envelope stress in glioblastoma. Kamikawa Y, Wu Z, Nakazawa N, Ito T, Saito A, Imaizumi K. Cell Death Discov. 2023 Jul 8;9(1):233. doi: 10.1038/s41420-023-01534-7. PubMed
An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate. Hansen SL, Larsen HL, Pikkupeura LM, Maciag G, Guiu J, Muller I, Clement DL, Mueller C, Johansen JV, Helin K, Lerdrup M, Jensen KB. Sci Adv. 2023 Jul 14;9(28):eadg4055. doi: 10.1126/sciadv.adg4055. Epub 2023 Jul 12. PubMed
Context-defined cancer co-dependency mapping identifies a functional interplay between PRC2 and MLL-MEN1 complex in lymphoma. Chen X, Li Y, Zhu F, Xu X, Estrella B, Pazos MA 2nd, McGuire JT, Karagiannis D, Sahu V, Mustafokulov M, Scuoppo C, Sanchez-Rivera FJ, Soto-Feliciano YM, Pasqualucci L, Ciccia A, Amengual JE, Lu C. Nat Commun. 2023 Jul 17;14(1):4259. doi: 10.1038/s41467-023-39990-5. PubMed
APOBEC3 activity promotes the survival and evolution of drug-tolerant persister cells during acquired resistance to EGFR inhibitors in lung cancer. Garcia NMG, Becerra JN, McKinney BJ, DiMarco AV, Wu F, Fitzgibbon M, Alvarez JV. bioRxiv. 2023 Jul 3:2023.07.02.547443. doi: 10.1101/2023.07.02.547443. Preprint. PubMed
Coding and non-coding elements comprise a regulatory network controlling transcription in Kaposi's sarcoma-associated herpesvirus. Morgens DW, Gulyas L, Souza AS, Glaunsinger BA. bioRxiv. 2023 Jul 8:2023.07.08.548212. doi: 10.1101/2023.07.08.548212. Preprint. PubMed
SF3B1 mutation and ATM deletion codrive leukemogenesis via centromeric R-loop dysregulation. Cusan M, Shen H, Zhang B, Liao A, Yang L, Jin M, Fernandez M, Iyer P, Wu Y, Hart K, Gutierrez C, Nik S, Pruett-Miller SM, Stark J, Obeng EA, Bowman TV, Wu CJ, Lin RJ, Wang L. J Clin Invest. 2023 Sep 1;133(17):e163325. doi: 10.1172/JCI163325. PubMed
Crosstalk between RNA m(6)A and DNA methylation regulates transposable element chromatin activation and cell fate in human pluripotent stem cells. Sun T, Xu Y, Xiang Y, Ou J, Soderblom EJ, Diao Y. Nat Genet. 2023 Aug;55(8):1324-1335. doi: 10.1038/s41588-023-01452-5. Epub 2023 Jul 20. PubMed
NLRP11 is a pattern recognition receptor for bacterial lipopolysaccharide in the cytosol of human macrophages. Rojas-Lopez M, Gil-Marques ML, Kharbanda V, Zajac AS, Miller KA, Wood TE, Hachey AC, Egger KT, Goldberg MB. Sci Immunol. 2023 Jul 21;8(85):eabo4767. doi: 10.1126/sciimmunol.abo4767. Epub 2023 Jul 21. PubMed
Genome-scale CRISPR screen identifies TRIM2 and SLC35A1 associated with porcine epidemic diarrhoea virus infection. Wang J, Liu H, Yang Y, Tan Y, Sun L, Guo Z, Zeng X, Wang Z, Li S, Yin L, Yin D, Shen X, Dai Y, Liu X, Ruan J, Li X, Zhao S, Peng G, Pan X, Wang C, Xie S. Int J Biol Macromol. 2023 Jul 25;250:125962. doi: 10.1016/j.ijbiomac.2023.125962. PubMed
NSD2 maintains lineage plasticity and castration-resistance in neuroendocrine prostate cancer. Li JJ, Vasciaveo A, Karagiannis D, Sun Z, Chen X, Socciarelli F, Frankenstein Z, Zou M, Pannellini T, Chen Y, Gardner K, Robinson BD, de Bono J, Abate-Shen C, Rubin MA, Loda M, Sawyers CL, Califano A, Lu C, Shen MM. bioRxiv. 2023 Jul 19:2023.07.18.549585. doi: 10.1101/2023.07.18.549585. Preprint. PubMed
Pooled tagging and hydrophobic targeting of endogenous proteins for unbiased mapping of unfolded protein responses. Sansbury SE, Serebrenik YV, Lapidot T, Burslem GM, Shalem O. bioRxiv. 2023 Jul 14:2023.07.13.548611. doi: 10.1101/2023.07.13.548611. Preprint. PubMed
Systematic characterization of therapeutic vulnerabilities in Multiple Myeloma with Amp1q reveals increased sensitivity to the combination of MCL1 and PI3K inhibitors. Sklavenitis-Pistofidis R, Lightbody ED, Reidy M, Tsuji J, Aranha MP, Heilpern-Mallory D, Huynh D, Chong SJF, Hackett L, Haradhvala NJ, Wu T, Su NK, Berrios B, Alberge JB, Dutta A, Davids MS, Papaioannou M, Getz G, Ghobrial IM, Manier S. bioRxiv [Preprint]. 2023 Aug 3:2023.08.01.551480. doi: 10.1101/2023.08.01.551480. PubMed
The oncogenic human B-cell lymphoma MYD88 L265P mutation genocopies activation by phosphorylation at the Toll/interleukin-1 receptor (TIR) domain. Minderman M, Lantermans H, van der Zwaan C, Hoogendijk AJ, van den Biggelaar M, Kersten MJ, Spaargaren M, Pals ST. Blood Cancer J. 2023 Aug 18;13(1):125. doi: 10.1038/s41408-023-00896-6. PubMed
OTUB1 inhibits breast cancer by non-canonically stabilizing CCN6. Zhao Y, Ruan J, Li Z, Su X, Chen K, Lin Y, Cai Y, Wang P, Liu B, Schluter D, Liang G, Wang X. Clin Transl Med. 2023 Aug;13(8):e1385. doi: 10.1002/ctm2.1385. PubMed
NKG2A gene variant predicts outcome of immunotherapy in AML and modulates the repertoire and function of NK cells. Hussein BA, Kristenson L, Pesce S, Wohr A, Tian Y, Hallner A, Brune M, Hellstrand K, Tang KW, Bernson E, Thoren FB. J Immunother Cancer. 2023 Aug;11(8):e007202. doi: 10.1136/jitc-2023-007202. PubMed
Betacoronaviruses SARS-CoV-2 and HCoV-OC43 infections in IGROV-1 cell line require aryl hydrocarbon receptor. Yousefi M, Lee WS, Chan WOY, He W, Mah MG, Yong CL, Deerain JM, Wang L, Arcinas C, Yan B, Tan D, Sia WR, Gamage AM, Yang J, Hsu AC, Li S, Linster M, Yang X, Ghosh S, Anderson DE, Smith GJD, Tan CW, Wang LF, Ooi YS. Emerg Microbes Infect. 2023 Dec;12(2):2256416. doi: 10.1080/22221751.2023.2256416. Epub 2023 Sep 6. PubMed
Modulation of the proteostasis network promotes tumor resistance to oncogenic KRAS inhibitors. Lv X, Lu X, Cao J, Luo Q, Ding Y, Peng F, Pataer A, Lu D, Han D, Malmberg E, Chan DW, Wang X, Savage SR, Mao S, Yu J, Peng F, Yan L, Meng H, Maneix L, Han Y, Chen Y, Yao W, Chang EC, Catic A, Lin X, Miles G, Huang P, Sun Z, Burt B, Wang H, Wang J, Yao QC, Zhang B, Roth JA, O'Malley BW, Ellis MJ, Rimawi MF, Ying H, Chen X. Science. 2023 Sep 8;381(6662):eabn4180. doi: 10.1126/science.abn4180. Epub 2023 Sep 8. PubMed
Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunity. Cui Y, Miao Y, Cao L, Guo L, Cui Y, Yan C, Zeng Z, Xu M, Han T. Nat Commun. 2023 Sep 15;14(1):5740. doi: 10.1038/s41467-023-41101-3. PubMed
Aberrant gene activation in synovial sarcoma relies on SSX specificity and increased PRC1.1 stability. Benabdallah NS, Dalal V, Scott RW, Marcous F, Sotiriou A, Kommoss FKF, Pejkovska A, Gaspar L, Wagner L, Sanchez-Rivera FJ, Ta M, Thornton S, Nielsen TO, Underhill TM, Banito A. Nat Struct Mol Biol. 2023 Nov;30(11):1640-1652. doi: 10.1038/s41594-023-01096-3. Epub 2023 Sep 21. PubMed
CRISPR-Cas9 knockout screening identifies KIAA1429 as an essential gene in Ewing sarcoma. Tan K, Lu W, Chen F, Shi H, Ma Y, Chen Z, Wu W, Lv Z, Mo J. J Exp Clin Cancer Res. 2023 Sep 28;42(1):250. doi: 10.1186/s13046-023-02828-5. PubMed
Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression. Kramer NJ, Prakash G, Isaac RS, Choquet K, Soto I, Petrova B, Merens HE, Kanarek N, Churchman LS. Nat Cell Biol. 2023 Nov;25(11):1575-1589. doi: 10.1038/s41556-023-01244-3. Epub 2023 Sep 28. PubMed
TET2 lesions enhance the aggressiveness of CEBPA-mutant acute myeloid leukemia by rebalancing GATA2 expression. Heyes E, Wilhelmson AS, Wenzel A, Manhart G, Eder T, Schuster MB, Rzepa E, Pundhir S, D'Altri T, Frank AK, Gentil C, Woessmann J, Schoof EM, Meggendorfer M, Schwaller J, Haferlach T, Grebien F, Porse BT. Nat Commun. 2023 Oct 4;14(1):6185. doi: 10.1038/s41467-023-41927-x. PubMed
Distinct and targetable role of calcium-sensing receptor in leukaemia. Pereira RS, Kumar R, Cais A, Paulini L, Kahler A, Bravo J, Minciacchi VR, Krack T, Kowarz E, Zanetti C, Godavarthy PS, Hoeller F, Llavona P, Stark T, Tascher G, Nowak D, Meduri E, Huntly BJP, Munch C, Pampaloni F, Marschalek R, Krause DS. Nat Commun. 2023 Oct 6;14(1):6242. doi: 10.1038/s41467-023-41770-0. PubMed
Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status. Poohadsuan J, O'Doherty GA, Owattanapanich W, Kungwankiattichai S, Rojanasakul Y, Issaragrisil S, Luanpitpong S. Cell Commun Signal. 2023 Oct 12;21(1):283. doi: 10.1186/s12964-023-01317-8. PubMed
Landscape of enhancer disruption and functional screen in melanoma cells. Wang Z, Luo M, Liang Q, Zhao K, Hu Y, Wang W, Feng X, Hu B, Teng J, You T, Li R, Bao Z, Pan W, Yang T, Zhang C, Li T, Dong X, Yi X, Liu B, Zhao L, Li M, Chen K, Song W, Yang J, Li MJ. Genome Biol. 2023 Oct 30;24(1):248. doi: 10.1186/s13059-023-03087-5. PubMed
MIG6 loss confers resistance to ALK/ROS1 inhibitors in NSCLC through EGFR activation by low-dose EGF. Kondo N, Utsumi T, Shimizu Y, Takemoto A, Oh-Hara T, Uchibori K, Subat-Motoshi S, Ninomiya H, Takeuchi K, Nishio M, Miyazaki Y, Katayama R. JCI Insight. 2023 Dec 22;8(24):e173688. doi: 10.1172/jci.insight.173688. PubMed
Identification of host essential factors for recombinant AAV transduction of the polarized human airway epithelium. Hao S, Zhang X, Ning K, Feng Z, Park SY, Aksu Kuz C, McFarlin S, Richart D, Cheng F, Zhang EY, Zhang-Chen A, Yan Z, Qiu J. J Virol. 2023 Dec 21;97(12):e0133023. doi: 10.1128/jvi.01330-23. Epub 2023 Nov 15. PubMed
Dual role of CASP8AP2/FLASH in regulating epithelial-to-mesenchymal transition plasticity (EMP). Catalanotto M, Vaz JM, Abshire C, Youngblood R, Chu M, Levine H, Jolly MK, Dragoi AM. Transl Oncol. 2024 Jan;39:101837. doi: 10.1016/j.tranon.2023.101837. Epub 2023 Nov 18. PubMed
BioE3 identifies specific substrates of ubiquitin E3 ligases. Barroso-Gomila O, Merino-Cacho L, Muratore V, Perez C, Taibi V, Maspero E, Azkargorta M, Iloro I, Trulsson F, Vertegaal ACO, Mayor U, Elortza F, Polo S, Barrio R, Sutherland JD. Nat Commun. 2023 Nov 23;14(1):7656. doi: 10.1038/s41467-023-43326-8. PubMed Associated Plasmids TRIPZ-bioGEF-SUMO1nc-PURO TRIPZ-bioGEF-SUMO2nc-PURO TRIPZ-bioGEF-UBnc-PURO TRIPZ-bioGEF-UBwt-PURO Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST More ...
Immunoproteasome function maintains oncogenic gene expression in KMT2A-complex driven leukemia. Tubio-Santamaria N, Jayavelu AK, Schnoeder TM, Eifert T, Hsu CJ, Perner F, Zhang Q, Wenge DV, Hansen FM, Kirkpatrick JM, Jyotsana N, Lane SW, von Eyss B, Deshpande AJ, Kuhn MWM, Schwaller J, Cammann C, Seifert U, Ebstein F, Kruger E, Hochhaus A, Heuser M, Ori A, Mann M, Armstrong SA, Heidel FH. Mol Cancer. 2023 Dec 4;22(1):196. doi: 10.1186/s12943-023-01907-7. PubMed
Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells. Maimaitili M, Chen M, Febbraro F, Ucuncu E, Kelly R, Niclis JC, Christiansen JR, Mermet-Joret N, Niculescu D, Lauritsen J, Iannielli A, Klaestrup IH, Jensen UB, Qvist P, Nabavi S, Broccoli V, Nykjaer A, Romero-Ramos M, Denham M. Nat Commun. 2023 Dec 5;14(1):7871. doi: 10.1038/s41467-023-43471-0. PubMed Associated Plasmids pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP pLV-4gRNA-GBX2-RFP phH1-GBX2-gRNA phU6-CDX4-gRNA pmU6-CDX1-gRNA More ...
Leukemia-intrinsic determinants of CAR-T response revealed by iterative in vivo genome-wide CRISPR screening. Ramos A, Koch CE, Liu-Lupo Y, Hellinger RD, Kyung T, Abbott KL, Frose J, Goulet D, Gordon KS, Eidell KP, Leclerc P, Whittaker CA, Larson RC, Muscato AJ, Yates KB, Dubrot J, Doench JG, Regev A, Vander Heiden MG, Maus MV, Manguso RT, Birnbaum ME, Hemann MT. Nat Commun. 2023 Dec 5;14(1):8048. doi: 10.1038/s41467-023-43790-2. PubMed
The MYC-Regulated RNA-Binding Proteins hnRNPC and LARP1 Are Drivers of Multiple Myeloma Cell Growth and Disease Progression and Negatively Predict Patient Survival. Seibert M, Koschade SE, Stolp V, Haupl B, Wempe F, Serve H, Kurrle N, Schnutgen F, von Metzler I. Cancers (Basel). 2023 Nov 21;15(23):5508. doi: 10.3390/cancers15235508. PubMed
Hyperactive Natural Killer cells in Rag2 knockout mice inhibit the development of acute myeloid leukemia. Sugimoto E, Li J, Hayashi Y, Iida K, Asada S, Fukushima T, Tamura M, Shikata S, Zhang W, Yamamoto K, Kawabata KC, Kawase T, Saito T, Yoshida T, Yamazaki S, Kaito Y, Imai Y, Denda T, Ota Y, Fukuyama T, Tanaka Y, Enomoto Y, Kitamura T, Goyama S. Commun Biol. 2023 Dec 21;6(1):1294. doi: 10.1038/s42003-023-05606-3. PubMed
Chromosome 8p engineering reveals increased metastatic potential targetable by patient-specific synthetic lethality in liver cancer. Huth T, Dreher EC, Lemke S, Fritzsche S, Sugiyanto RN, Castven D, Ibberson D, Sticht C, Eiteneuer E, Jauch A, Pusch S, Albrecht T, Goeppert B, Candia J, Wang XW, Ji J, Marquardt JU, Nahnsen S, Schirmacher P, Roessler S. Sci Adv. 2023 Dec 22;9(51):eadh1442. doi: 10.1126/sciadv.adh1442. Epub 2023 Dec 22. PubMed

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