pHPHS800
(Plasmid
#228240)
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PurposeBxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination
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Depositing Lab
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Depositing OrganizationFred Hutchinson Cancer Center
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 228240-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 228240-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneYTK089
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9
- Promoter none
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Tag
/ Fusion Protein
- Hygromycin phosphotransferase
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ggccagcttggcacttg
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.25.605204 for bioRxiv preprint.
DNA (Catalog # 228240-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 228240-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHPHS800 was a gift from Arvind Rasi Subramaniam (Addgene plasmid # 228240 ; http://n2t.net/addgene:228240 ; RRID:Addgene_228240) -
For your References section:
Decoding post-transcriptional regulatory networks by RNA-linked CRISPR screening in human cells. Nugent PJ, Park H, Wladyka CL, Yelland JN, Sinha S, Chen KY, Bynum C, Quarterman G, Lee SC, Hsieh AC, Subramaniam AR. Nat Methods. 2025 Jun;22(6):1237-1246. doi: 10.1038/s41592-025-02702-6. Epub 2025 May 29. 10.1038/s41592-025-02702-6 PubMed 40442371
