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CRL4(AMBRA1) is a master regulator of D-type cyclins.

Simoneschi D, Rona G, Zhou N, Jeong YT, Jiang S, Milletti G, Arbini AA, O'Sullivan A, Wang AA, Nithikasem S, Keegan S, Siu Y, Cianfanelli V, Maiani E, Nazio F, Cecconi F, Boccalatte F, Fenyo D, Jones DR, Busino L, Pagano M
Nature. 2021 Apr;592(7856):789-793. doi: 10.1038/s41586-021-03445-y. Epub 2021 Apr 14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
172603pBABE-MCS(extended)-Puro (empty vector)Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.
172604pcDNA3.1-2xFLAG-2xSTREP (empty vector)Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells
172605pcDNA3.1-AMBRA1-3xFLAGExpresses 3xFLAG-tagged AMBRA1 in mammalian cells
172606px459-AMBRA1 #3Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-Puro
172607px458-AMBRA1 #3Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP
172608px458-AMBRA1 (optimized for N-terminal knock-in)Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.
172609pUC57-2xFLAG-mAID-AMBRA1 HDR donor templateDonor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene
172610pTRIPZ-TIR1-HALentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells
172611pMSCV-FLAG-2xSTREP-CDKN1B-PuroRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells
172612pcDNA3.1-HA-CDK4Expresses HA-tagged CDK4 in mammalian cells
172613pcDNA3.1-HA-CDK2Expresses HA-tagged CDK2 in mammalian cells
172614pcDNA3.1-2xFLAG-2xSTREP-CDK6Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells
172615pcDNA3.1-2xFLAG-2xSTREP-CDK4Expresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cells
172616pcDNA3.1-2xFLAG-2xSTREP-CDK2Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells
172617pMSCV-FLAG-2xSTREP-CCND3(P284L)-PuroRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(P284L) and a puromycin resistance marker in mammalian cells
172618pMSCV-FLAG-2xSTREP-CCND3(T283A)-PuroRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells
172619pMSCV-FLAG-2xSTREP-CCND3-PuroRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells
172620pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherryLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells
172621pcDNA3.1-HA-CCND3Expresses HA-tagged cyclin D3 in mammalian cells
172622pBABE-2xFLAG-2xSTREP-CCND3-PuroRetroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells
172623pcDNA3.1-2xFLAG-2xSTREP-CCND3Expresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cells
172624pMSCV-FLAG-2xSTREP-CCND2(P281L)-PuroRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(P281L) and a puromycin resistance marker in mammalian cells
172625pMSCV-FLAG-2xSTREP-CCND2(T280A)-PuroRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(T280A) and a puromycin resistance marker in mammalian cells
172626pMSCV-FLAG-2xSTREP-CCND2-PuroRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells
172627pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherryLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells
172628pBABE-2xFLAG-2xSTREP-CCND2-PuroRetroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells
172629pcDNA3.1-2xFLAG-2xSTREP-CCND2Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells
172630px458-CCND1Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP
172631pcDNA3.1-CCND1(T286A)Expresses untagged cyclin D1(T286A) in mammalian cells
172632pcDNA3.1-CCND1Expresses untagged cyclin D1 in mammalian cells
172633pBABE-2xFLAG-2xSTREP-CCND1(T286A)-PuroRetroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells
172634pBABE-2xFLAG-2xSTREP-CCND1-PuroRetroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells
172635pLVX-2xFLAG-2xSTREP-CCND1(P287S)-IRES-mCherryLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287S) and mCherry in mammalian cells
172636pLVX-2xFLAG-2xSTREP-CCND1(P287L)-IRES-mCherryLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287L) and mCherry in mammalian cells
172637pLVX-2xFLAG-2xSTREP-CCND1(P287A)-IRES-mCherryLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287A) and mCherry in mammalian cells
172638pLVX-2xFLAG-2xSTREP-CCND1(T286I)-IRES-mCherryLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286I) and mCherry in mammalian cells
172639pLVX-2xFLAG-2xSTREP-CCND1(T286A)-IRES-mCherryLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and mCherry in mammalian cells
172640pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherryLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cells
172641pMSCV-FLAG-2xSTREP-CCND1(P287A)-PuroRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(P287A) and a puromycin resistance marker in mammalian cells
172642pMSCV-FLAG-2xSTREP-CCND1(T286A)-PuroRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells
172643pMSCV-FLAG-2xSTREP-CCND1-PuroRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells
172644pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287S)Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287S) in mammalian cells
172645pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286I)Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286I) in mammalian cells
172646pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287L)Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287L) in mammalian cells
172647pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells
172648pcDNA3.1-2xFLAG-2xSTREP-CCND1Expresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cells
172649pcDNA3.1-HA-CCND1Expresses HA-tagged cyclin D1 in mammalian cells

Antibodies from Article