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Engineered mini H1 promoters with dedicated RNA Polymerase II or III activity.
Gao Z, van der Velden YU, Fan M, van der Linden CA, Vink M, Herrera-Carrillo E, Berkhout B
J Biol Chem. 2020 Nov 5. pii: S0021-9258(20)00012-5. doi: 10.1074/jbc.RA120.015386.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
193663H1.99.lucExpresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99)
193664H1.99.M.lucExpresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99) with the TATA box mutated
193704H1.224.lucExpresses a truncated PolII H1 promoter (H1.244)
193705H1.224.M.lucExpresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated
193706H1.M10.lucExpressed a H1-core mutant by introducing multiple 7SK elements (PSE, spacer, and TATA box)
193707H1.M11.lucExpressed a H1-core mutant by introducing multiple 7SK elements (octamer, PSE, spacer, and TATA box)

Antibodies from Article