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Purpose
This lentiviral sgRNA library tiles the entire ~154 kb of the BAC16 Kaposi’s Sarcoma-associated Herpes Virus genome. A guide every ~8 bp allows pooled screening of the viral genome.
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Vector Backbone
pMCB320 - does not express Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 180272 | KSHV Tiling gRNA pooled library† | 1 | $ 540 | Add to Cart | |
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with plasmids expressing Cas9 such as lentiCas9-Blast (Addgene #52962) or otherwise used with cell lines already expressing Cas9.
Library Details
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SpeciesKaposi’s Sarcoma-associated Herpes Virus
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gRNAs24,000
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Controls1,000 non-targeting guides; 273 human safe-targeting guides
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume40 µL
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Concentration25 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KSHV Tiling Library was a gift from Britt Glaunsinger (Addgene #180272) -
For your References section:
A Two-tiered functional screen identifies herpesviral transcriptional modifiers and their essential domains. Morgens DW, Nandakumar D, Didychuk AL, Yang KJ, Glaunsinger BA. PLoS Pathog. 2022 Jan 18;18(1):e1010236. doi: 10.1371/journal.ppat.1010236. PubMed 35041709